Several mechanisms are developed by the microorganisms to tolerate few high concentrations of heavy metals. One of these mechanisms dependent upon anabolic and catabolic energy of microorganisms is the bioaccumulation...Several mechanisms are developed by the microorganisms to tolerate few high concentrations of heavy metals. One of these mechanisms dependent upon anabolic and catabolic energy of microorganisms is the bioaccumulation of heavy metals. In present work, approximately four varieties of bacteria have been isolated from the ash dyke sample of four thermal power plants of Chhattisgarh, i.e., Bharat Aluminium Company (BALCO), Chhattisgarh State Electricity Board (CSEB), Korba, Thermal Power Cooperation (NTPC), Bilaspur and KSK Akaltara, Chattisgarh. Out of one hundred fifty isolates, three were capable to grow in varying concentration of heavy metals. The strains were tested for their tolerance against six different types of heavy metals dominant in the ash samples viz. Pb, Hg, Ni, Co, Cu, Mn. Their maximum resistance existed up to 0.6mM/ml of the above mentioned different metals under lab standard conditions. Three isolates are found suitable for the multiple metal resistance ability viz SM2, SM3, and SM12. These are categorized as Bacillus cereus (SM2, SM3), and Bacillus subtilis (SM12) after performing 16S rDNA sequencing.展开更多
The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated...The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated not only microbial diversity but also drug resistant microbes largely habitat in ICU.Pseudomonas aeruginosa found as a part of normal intestinal flora and a significant pathogen responsible for wide range of ICU acquired infection in critically ill patients.Nosocomial infection associated with this organism including gastrointestinal infection,urinary tract infections and blood stream infection.Infection caused by this organism are difficult to treat because of the presence of its innate resistance to many antibiotics(b-lactam and penem group of antibiotics),and its ability to acquire further resistance mechanism to multiple class of antibiotics,including Beta-lactams,aminoglycosides and fluoroquinolones.In the molecular evolution microbes adopted several mechanism to maintain genomic plasticity.The tool microbe use for its survival is mainly biofilm formation,quorum sensing,and horizontal gene transfer and enzyme promiscuity.Such genomic plasticity provide an ideal habitat to grow and survive in hearse environment mainly antibiotics pressure.This review focus on infection caused by Pseudomonas aeruginosa,its mechanisms of resistance and available treatment options.The present study provides a systemic review on major source of Pseudomonas aeruginosa in ICU.Further,study also emphasizes virulence gene/s associated with Pseudomonas aeruginosa genome for extended drug resistance.Study gives detailed overview of antibiotic drug resistance mechanism.展开更多
文摘Several mechanisms are developed by the microorganisms to tolerate few high concentrations of heavy metals. One of these mechanisms dependent upon anabolic and catabolic energy of microorganisms is the bioaccumulation of heavy metals. In present work, approximately four varieties of bacteria have been isolated from the ash dyke sample of four thermal power plants of Chhattisgarh, i.e., Bharat Aluminium Company (BALCO), Chhattisgarh State Electricity Board (CSEB), Korba, Thermal Power Cooperation (NTPC), Bilaspur and KSK Akaltara, Chattisgarh. Out of one hundred fifty isolates, three were capable to grow in varying concentration of heavy metals. The strains were tested for their tolerance against six different types of heavy metals dominant in the ash samples viz. Pb, Hg, Ni, Co, Cu, Mn. Their maximum resistance existed up to 0.6mM/ml of the above mentioned different metals under lab standard conditions. Three isolates are found suitable for the multiple metal resistance ability viz SM2, SM3, and SM12. These are categorized as Bacillus cereus (SM2, SM3), and Bacillus subtilis (SM12) after performing 16S rDNA sequencing.
文摘The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated not only microbial diversity but also drug resistant microbes largely habitat in ICU.Pseudomonas aeruginosa found as a part of normal intestinal flora and a significant pathogen responsible for wide range of ICU acquired infection in critically ill patients.Nosocomial infection associated with this organism including gastrointestinal infection,urinary tract infections and blood stream infection.Infection caused by this organism are difficult to treat because of the presence of its innate resistance to many antibiotics(b-lactam and penem group of antibiotics),and its ability to acquire further resistance mechanism to multiple class of antibiotics,including Beta-lactams,aminoglycosides and fluoroquinolones.In the molecular evolution microbes adopted several mechanism to maintain genomic plasticity.The tool microbe use for its survival is mainly biofilm formation,quorum sensing,and horizontal gene transfer and enzyme promiscuity.Such genomic plasticity provide an ideal habitat to grow and survive in hearse environment mainly antibiotics pressure.This review focus on infection caused by Pseudomonas aeruginosa,its mechanisms of resistance and available treatment options.The present study provides a systemic review on major source of Pseudomonas aeruginosa in ICU.Further,study also emphasizes virulence gene/s associated with Pseudomonas aeruginosa genome for extended drug resistance.Study gives detailed overview of antibiotic drug resistance mechanism.
