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DNA Barcode ITS Effectively Distinguishes the Medicinal Plant Boerhavia diffusa from Its Adulterants 被引量:3
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作者 Dhivya Selvaraj Dhivya Shanmughanandhan +3 位作者 rajeev kumar sarma Jijo C. Joseph Ramachandran V. Srinivasan Sathishkumar Ramalingam 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2012年第6期364-367,共4页
Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb i... Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug's efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that 1TS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products. 展开更多
关键词 ADULTERANT Boerhavia diffusa ITS DNA barcoding Punarnava
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