AIM: To describe and compare corneal sensation and morphological changes of sub-basal corneal nerves by in vivo laser scanning confocal microscopy(LSCM) in herpes simplex virus(HSV) keratitis/uveitis and contralateral...AIM: To describe and compare corneal sensation and morphological changes of sub-basal corneal nerves by in vivo laser scanning confocal microscopy(LSCM) in herpes simplex virus(HSV) keratitis/uveitis and contralateral, clinically unaffected eyes. METHODS: A prospective clinical study included 30 HSV eyes and 30 contralateral eyes of 30 patients, diagnosed with unilateral HSV keratitis/uveitis. Both eyes underwent a complete ophthalmological examination, Cochet-Bonnet aesthesiometry and LSCM of the central cornea, using the Heidelberg Retina Tomograph III Rostock Cornea Module. After 6 mo, the same examination of the HSV affected and contralateral, clinically unaffected eyes was performed.RESULTS: HSV eyes, as compared to contralateral eyes, demonstrated a significant decrease in mean corneal sensation(3.1±1.6 vs 5.3±0.8 cm), total nerve fibres number(5.7±4.4 vs 15.1±5.4), nerve branches(3.4±3.0 vs 8.4±4.7), main nerve trunks(2.3±1.6 vs 5.8±2.2), and nerve fibres density(7.5±5.6 vs 18.1±5.3 mm/mm2, P<0.05). There was no significant difference between keratitis and uveitis eyes in mean corneal sensation and nerve fibres parameters. After 6 mo, corneal sensation and sub-basal nerve fibres parameters were increased significantly, but did not reach the parameters of contralateral, clinically unaffected eyes.CONCLUSION: Corneal aesthesiometry and LSCM in HSV affected eyes reveals a significant decrease of corneal sensation and sub-basal nerve fibres which recovers at6 mo but does not reach the normal level.展开更多
AIM: To examine the MMP-2(-1306 C/T) gene polymorphism and the phenotype characterized by soft and hard drusen of early age-related macular degeneration(AMD) and geographic atrophy of late AMD form.METHODS: The study ...AIM: To examine the MMP-2(-1306 C/T) gene polymorphism and the phenotype characterized by soft and hard drusen of early age-related macular degeneration(AMD) and geographic atrophy of late AMD form.METHODS: The study enrolled 850 investigations(290 AMD patients with soft and hard drusen, 34 with geographic atrophy and a random sample of the population n=526). Early AMD was classified according to the International Classification and Grading System. For geographic atrophy diagnosis the Age-Related Eye Disease Study classification was used. The potential association with single nucleotide polymorphisms on MMP-2 Rs243865 was evaluated for all patients, adjusted for age and sex. The genotyping test of MMP-2 Rs243865(C-->T) was conducted using the real-time polymerase chain reaction method.RESULTS: MMP-2(-1306 C/T) C/C genotype was more frequently detected in AMD patients with hard drusen than the soft drusen or control group(66.43% vs 53.74%, vs 54.94%, P=0.047). Logistic regression analysis showed that the MMP-2(-1306) C/C genotype increased the likelihood to develop hard drusen in AMD patients(OR=1.7, 95% CI: 1.06-2.74; P=0.028). No association between MMP-2(-1306 C/T) gene polymorphism in patients with atrophic AMD and control group was found(54.94%, 37.64%, 7.41% vs 50%, 38.24%, 11.76%; P=0.6).CONCLUSION: The MMP-2 Rs24386(C-->T) polymorphism is found to be associated with the development of hard drusen in patients with AMD.展开更多
文摘AIM: To describe and compare corneal sensation and morphological changes of sub-basal corneal nerves by in vivo laser scanning confocal microscopy(LSCM) in herpes simplex virus(HSV) keratitis/uveitis and contralateral, clinically unaffected eyes. METHODS: A prospective clinical study included 30 HSV eyes and 30 contralateral eyes of 30 patients, diagnosed with unilateral HSV keratitis/uveitis. Both eyes underwent a complete ophthalmological examination, Cochet-Bonnet aesthesiometry and LSCM of the central cornea, using the Heidelberg Retina Tomograph III Rostock Cornea Module. After 6 mo, the same examination of the HSV affected and contralateral, clinically unaffected eyes was performed.RESULTS: HSV eyes, as compared to contralateral eyes, demonstrated a significant decrease in mean corneal sensation(3.1±1.6 vs 5.3±0.8 cm), total nerve fibres number(5.7±4.4 vs 15.1±5.4), nerve branches(3.4±3.0 vs 8.4±4.7), main nerve trunks(2.3±1.6 vs 5.8±2.2), and nerve fibres density(7.5±5.6 vs 18.1±5.3 mm/mm2, P<0.05). There was no significant difference between keratitis and uveitis eyes in mean corneal sensation and nerve fibres parameters. After 6 mo, corneal sensation and sub-basal nerve fibres parameters were increased significantly, but did not reach the parameters of contralateral, clinically unaffected eyes.CONCLUSION: Corneal aesthesiometry and LSCM in HSV affected eyes reveals a significant decrease of corneal sensation and sub-basal nerve fibres which recovers at6 mo but does not reach the normal level.
基金Supported by Lithuanian Science Council(No.MIP-10330)
文摘AIM: To examine the MMP-2(-1306 C/T) gene polymorphism and the phenotype characterized by soft and hard drusen of early age-related macular degeneration(AMD) and geographic atrophy of late AMD form.METHODS: The study enrolled 850 investigations(290 AMD patients with soft and hard drusen, 34 with geographic atrophy and a random sample of the population n=526). Early AMD was classified according to the International Classification and Grading System. For geographic atrophy diagnosis the Age-Related Eye Disease Study classification was used. The potential association with single nucleotide polymorphisms on MMP-2 Rs243865 was evaluated for all patients, adjusted for age and sex. The genotyping test of MMP-2 Rs243865(C-->T) was conducted using the real-time polymerase chain reaction method.RESULTS: MMP-2(-1306 C/T) C/C genotype was more frequently detected in AMD patients with hard drusen than the soft drusen or control group(66.43% vs 53.74%, vs 54.94%, P=0.047). Logistic regression analysis showed that the MMP-2(-1306) C/C genotype increased the likelihood to develop hard drusen in AMD patients(OR=1.7, 95% CI: 1.06-2.74; P=0.028). No association between MMP-2(-1306 C/T) gene polymorphism in patients with atrophic AMD and control group was found(54.94%, 37.64%, 7.41% vs 50%, 38.24%, 11.76%; P=0.6).CONCLUSION: The MMP-2 Rs24386(C-->T) polymorphism is found to be associated with the development of hard drusen in patients with AMD.
