Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbo...Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.展开更多
Aim The purpose of this study was to conduct quantitative research on bone height and bone mineral density of palatal implant sites for implantation, and to provide reference sites for safe and stable palatal implants...Aim The purpose of this study was to conduct quantitative research on bone height and bone mineral density of palatal implant sites for implantation, and to provide reference sites for safe and stable palatal implants. Methodology Three-dimensional reformatting images were reconstructed by cone beam computed tomography (CBCT) in 34 patients, aged 18 to 35 years, using EZ Implant software. Bone height was measured at 20 sites of interest on the palate. Bone mineral density was measured at the 10 sites with the highest implantation rate, classified using K-mean cluster analysis based on bone height and bone mineral density. Results According to the cluster analysis, 10 sites were classified into three clusters. Significant differences in bone height and bone mineral density were detected between these three clusters (P<0.05). The greatest bone height was obtained in cluster 2, followed by cluster 1 and cluster 3. The highest bone mineral density was found in cluster 3, followed by cluster 1 and cluster 2. Conclusion CBCT plays an important role in pre-surgical treatment planning. CBCT is helpful in identifying safe and stable implantation sites for palatal anchorage.展开更多
Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules b...Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules by wrapping morphogenesis protein bones-2(BMP-2)using chitosan nanoparticles,and compositing collagen materials.Methods:Twenty four SpragueDawley rats were randomly divided into four groups with six rats in each group,that is Group A(control group),Group B(only treated with collagen),Group C(rhBMP-2+collagen treated group) and Group D(rhBMP-2/cs+collagen treated group).The composite materials for each group were implanted in the bilateral peroneal muscle pouches in rats.The peroneal muscles were only separated without implanting any materials in control group.Rats were sacrificed 2 weeks and 4 weeks post treatment and samples were cut off for general observation,Micro CT scans and histological observation.Results:General observation showed no new bone formation in Groups A and B mice,while new bones were formed in Groups C and D mice.Two weeks after treatment Micro CT scans showed that The bone volume fraction(BVF),trabecular thickness(Tb. Th),bone mineral density(BMD) in Group C mice were all higher than that in Group D(P<0.05). At the fourth week,the BVK,Tb.Th and BMD were significantly higher than that at the second week(P<0.01).Conclusions:The slow-release effect of rhBMP-2/cs sustained-release capsules can significantly promote ectopic osteogenesis.Its bone formation effect is better than that of rhBMP-2 burst-release group.展开更多
Objective:To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2(rhBMP-2) and basic fibroblast growth factor(bFGF).Methods:24 KM male mice wer...Objective:To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2(rhBMP-2) and basic fibroblast growth factor(bFGF).Methods:24 KM male mice were randomly divided into 6 groups with 4 mice in each group,namely,Group A(control group),Group B(only treated with collagen),Group C(treated with2 ng bFGF+collagen).Group D(treated with 4μg rhBMP-2+collagen),Group E(treated with 4 μg rhBMP-2+2 ng bFGF+collagen) and Group F(treated with 4 μg rhBMP-2+4 ng bFGF+collagem.The composites were implanted into the intermuscular septum of hind legs mice:whereas in control group,intermuscular septum of mice was separated and no implantation was performed.General observation,detection of concentration of calcium content,micro computed tomography(Micro-CT).three-dimensional reconstruction scan.measurement of bone mineral density(BMD).bone volume fraction(BVF) and trabecular thickness(Tb.Th).as well as histological observation with HE staining and ALP and CD34 immumohistochemical staining were performed.Results:Ectopic osteogenesis was found in Groups D.F and F mice.The difference in concentration of calcium contents was statistically significant between Groups D and E(P<0.05),but insignificant between Groups E and F(P>0.05).Micro-CT and three-dimensional reconstruction revealed continuous newborn bone substance in external surface of ectopic bone formation,and the center of bone formation did not show obvious substantial filling by bone substance.The differences in BMD,BVF and Tb.Th were statistically significant between Groups D and E or F(P<0.01 or <0.05).HE staining showed that in Groups D.E and F.newborn bone substance was mainly located at the edge of ectopic bone formation,and the bone formation in Groups E and F was better than that in Group D.ALP and CD34 immumohistochemical staining revealed the positive expression mainly at the edge of ectopic bone formation,and area of positive expression in Groups E and F was larger than that in Groups D.Conclusions:rhBMP-2 possesses the capacity to induce ectopic osteogenesis independently,but bFGF does not have this ability;the combined application of rhBMP-2 and bFGF can enhance the synergetic effect on inducing ectopic osteogenesis.展开更多
基金Supported by the Research and Development Project of Scientific and Technological Industry of Guangdong Province(2011B080701053)
文摘Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.
基金supported by Scientific Research Foundation for Returned Scholars of the Ministry of Education of China (No. 245027)the Science and Technology Planning Program of Guangdong Province (No. 2006B35801004)
文摘Aim The purpose of this study was to conduct quantitative research on bone height and bone mineral density of palatal implant sites for implantation, and to provide reference sites for safe and stable palatal implants. Methodology Three-dimensional reformatting images were reconstructed by cone beam computed tomography (CBCT) in 34 patients, aged 18 to 35 years, using EZ Implant software. Bone height was measured at 20 sites of interest on the palate. Bone mineral density was measured at the 10 sites with the highest implantation rate, classified using K-mean cluster analysis based on bone height and bone mineral density. Results According to the cluster analysis, 10 sites were classified into three clusters. Significant differences in bone height and bone mineral density were detected between these three clusters (P<0.05). The greatest bone height was obtained in cluster 2, followed by cluster 1 and cluster 3. The highest bone mineral density was found in cluster 3, followed by cluster 1 and cluster 2. Conclusion CBCT plays an important role in pre-surgical treatment planning. CBCT is helpful in identifying safe and stable implantation sites for palatal anchorage.
基金supported by Guangdong Province Science and Technology Foundation,Guangdong,China(No:2011B080701053)
文摘Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules by wrapping morphogenesis protein bones-2(BMP-2)using chitosan nanoparticles,and compositing collagen materials.Methods:Twenty four SpragueDawley rats were randomly divided into four groups with six rats in each group,that is Group A(control group),Group B(only treated with collagen),Group C(rhBMP-2+collagen treated group) and Group D(rhBMP-2/cs+collagen treated group).The composite materials for each group were implanted in the bilateral peroneal muscle pouches in rats.The peroneal muscles were only separated without implanting any materials in control group.Rats were sacrificed 2 weeks and 4 weeks post treatment and samples were cut off for general observation,Micro CT scans and histological observation.Results:General observation showed no new bone formation in Groups A and B mice,while new bones were formed in Groups C and D mice.Two weeks after treatment Micro CT scans showed that The bone volume fraction(BVF),trabecular thickness(Tb. Th),bone mineral density(BMD) in Group C mice were all higher than that in Group D(P<0.05). At the fourth week,the BVK,Tb.Th and BMD were significantly higher than that at the second week(P<0.01).Conclusions:The slow-release effect of rhBMP-2/cs sustained-release capsules can significantly promote ectopic osteogenesis.Its bone formation effect is better than that of rhBMP-2 burst-release group.
基金supported by Guangdong Provincial Technology and Development Foundation:(No:2012B0617000911 & No:2011B080701053)
文摘Objective:To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2(rhBMP-2) and basic fibroblast growth factor(bFGF).Methods:24 KM male mice were randomly divided into 6 groups with 4 mice in each group,namely,Group A(control group),Group B(only treated with collagen),Group C(treated with2 ng bFGF+collagen).Group D(treated with 4μg rhBMP-2+collagen),Group E(treated with 4 μg rhBMP-2+2 ng bFGF+collagen) and Group F(treated with 4 μg rhBMP-2+4 ng bFGF+collagem.The composites were implanted into the intermuscular septum of hind legs mice:whereas in control group,intermuscular septum of mice was separated and no implantation was performed.General observation,detection of concentration of calcium content,micro computed tomography(Micro-CT).three-dimensional reconstruction scan.measurement of bone mineral density(BMD).bone volume fraction(BVF) and trabecular thickness(Tb.Th).as well as histological observation with HE staining and ALP and CD34 immumohistochemical staining were performed.Results:Ectopic osteogenesis was found in Groups D.F and F mice.The difference in concentration of calcium contents was statistically significant between Groups D and E(P<0.05),but insignificant between Groups E and F(P>0.05).Micro-CT and three-dimensional reconstruction revealed continuous newborn bone substance in external surface of ectopic bone formation,and the center of bone formation did not show obvious substantial filling by bone substance.The differences in BMD,BVF and Tb.Th were statistically significant between Groups D and E or F(P<0.01 or <0.05).HE staining showed that in Groups D.E and F.newborn bone substance was mainly located at the edge of ectopic bone formation,and the bone formation in Groups E and F was better than that in Group D.ALP and CD34 immumohistochemical staining revealed the positive expression mainly at the edge of ectopic bone formation,and area of positive expression in Groups E and F was larger than that in Groups D.Conclusions:rhBMP-2 possesses the capacity to induce ectopic osteogenesis independently,but bFGF does not have this ability;the combined application of rhBMP-2 and bFGF can enhance the synergetic effect on inducing ectopic osteogenesis.
