This study explored the antibiofilm efficacy of 3,3’-diindolylmethane(DIM)on Staphylococcus aureus and its disinfection on common food-contact surfaces.The minimum biofilm inhibitory concentration(MBIC)of DIM on S.au...This study explored the antibiofilm efficacy of 3,3’-diindolylmethane(DIM)on Staphylococcus aureus and its disinfection on common food-contact surfaces.The minimum biofilm inhibitory concentration(MBIC)of DIM on S.aureus was 62.5μmol/L,while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay.DIM in the concentration range of 31.2-62.5μmol/L demonstrated a dose-dependent antibiofilm activity to S.aureus,as confirmed by light microscopic(LM),confocal laser scanning microscopic(CLSM),and scanning electron microscopic(SEM)analyses.At DIM of62.5μmol/L,the biomass of S.aureus biofilm was significantly reduced by 97%and its average thickness by 58%(P<0.05).DIM of 62.5μmol/L inhibited the bacterial initial adhesion and proliferation,as well as cell motility;the release of extracellular DNA(eDNA)and extracellular polysaccharide(EPS)were reduced by 75%and 69%,respectively.DIM exhibited a strong inhibition to S.aureus biofilm formation on common food-contact surfaces,including 304 stainless steel,glass,and polyvinyl chloride(PVC)but not disperse the mature biofilm.Overall,our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S.aureus on common food-contact surfaces utilized during food processing.展开更多
Eliminating colloidal toxicity and enabling its intrinsic fluorescence in aggressive environmental conditions are the key challenges for commercializing hydrophobic cadmium based quantum dots(QD).Polyarylene ether nit...Eliminating colloidal toxicity and enabling its intrinsic fluorescence in aggressive environmental conditions are the key challenges for commercializing hydrophobic cadmium based quantum dots(QD).Polyarylene ether nitriles(PEN)are an example of super-engineering thermoplastics that possess a unique combination of thermal stability,intrinsic fluorescence,biocompatibility and distinct emulsion self-assembly feature.Herein,the co-self-assembly of amphiphilic PEN with hydrophobic CdSe@ZnS QD,confined in the three-dimensional(3D)oil-in-water emulsion droplets,has been explored tofabricate fluorescent microparticles(FMP).It was found that these FMP demonstrated good biocompatibility(cell viability above 90%),while exhibiting a fluorescence emission in aqueous solution that was retained(intensity retention ratio above 80%)within the whole pH range of 1-14,as well as,after being subjected to autoclaving at 120℃for 1 h.Interestingly,it was discovered that introduction of calcium ions in the emulsion self-assembly contributed to in-situ generation of phase changing nanoplates inside the FMP,which led to the photo-thermal modulated solid state fluorescence from drop-casted FMP film.Thanks to their versatile fluorescence,these FMP colloids were exploited as fluorescent probes for macrophages imaging,while micro-patterns with reversible changing of emission color were induced via thermal treatment and direct laser lithography.展开更多
基金supported by the National Key Research and Development Projects(2019YFE0103800)Sichuan Science and Technology Program(2019YFH0113,2021YFH0060,2021YFH0072,2021ZHFP0045,2021YFN0092)the Fundamental Research Funds for the Central Universities(2018CDLZ-07,2018CDPZH-9,2019CDPZH-23,2020CDLZ-17)。
文摘This study explored the antibiofilm efficacy of 3,3’-diindolylmethane(DIM)on Staphylococcus aureus and its disinfection on common food-contact surfaces.The minimum biofilm inhibitory concentration(MBIC)of DIM on S.aureus was 62.5μmol/L,while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay.DIM in the concentration range of 31.2-62.5μmol/L demonstrated a dose-dependent antibiofilm activity to S.aureus,as confirmed by light microscopic(LM),confocal laser scanning microscopic(CLSM),and scanning electron microscopic(SEM)analyses.At DIM of62.5μmol/L,the biomass of S.aureus biofilm was significantly reduced by 97%and its average thickness by 58%(P<0.05).DIM of 62.5μmol/L inhibited the bacterial initial adhesion and proliferation,as well as cell motility;the release of extracellular DNA(eDNA)and extracellular polysaccharide(EPS)were reduced by 75%and 69%,respectively.DIM exhibited a strong inhibition to S.aureus biofilm formation on common food-contact surfaces,including 304 stainless steel,glass,and polyvinyl chloride(PVC)but not disperse the mature biofilm.Overall,our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S.aureus on common food-contact surfaces utilized during food processing.
基金the National Natural Science Foundation of China(No.51403029)the Fundamental Research Funds for the Central Universities(No.ZYGX2019J026)+1 种基金Sichuan Science and Technology Program(No.2020YFG0100)International Science and Technology Cooperation Project from Chengdu municipal government(No.2019-GH02-00037-HZ).K.J.thanks Prof.Yanbo Li from the IoF of UESTC for his timely assistance in fluorescence lifetime measurement.
文摘Eliminating colloidal toxicity and enabling its intrinsic fluorescence in aggressive environmental conditions are the key challenges for commercializing hydrophobic cadmium based quantum dots(QD).Polyarylene ether nitriles(PEN)are an example of super-engineering thermoplastics that possess a unique combination of thermal stability,intrinsic fluorescence,biocompatibility and distinct emulsion self-assembly feature.Herein,the co-self-assembly of amphiphilic PEN with hydrophobic CdSe@ZnS QD,confined in the three-dimensional(3D)oil-in-water emulsion droplets,has been explored tofabricate fluorescent microparticles(FMP).It was found that these FMP demonstrated good biocompatibility(cell viability above 90%),while exhibiting a fluorescence emission in aqueous solution that was retained(intensity retention ratio above 80%)within the whole pH range of 1-14,as well as,after being subjected to autoclaving at 120℃for 1 h.Interestingly,it was discovered that introduction of calcium ions in the emulsion self-assembly contributed to in-situ generation of phase changing nanoplates inside the FMP,which led to the photo-thermal modulated solid state fluorescence from drop-casted FMP film.Thanks to their versatile fluorescence,these FMP colloids were exploited as fluorescent probes for macrophages imaging,while micro-patterns with reversible changing of emission color were induced via thermal treatment and direct laser lithography.
