Unspecific peroxygenases(UPOs, EC 1.11.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without cofactors. However, the enhancement of ...Unspecific peroxygenases(UPOs, EC 1.11.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without cofactors. However, the enhancement of the H_(2)O_(2) tolerance of UPOs is always challenging. In this study, the A161C mutant of r Dca UPO,which originates from Daldinia caldariorum, was found to be highly H_(2)O_(2)-resistant. Compared with the wild type, the mutant r Dca UPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H_(2)O_(2). The crystal structure analysis at 1.47 A showed that r Dca UPOA161C exhibited 10 α-helixes(cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two conserved domains near heme formed an active site with the catalytic PCP and EHD regions(Glu86, His87, Asp88 residues). The H_(2)O_(2) tolerance of r Dca UPO-A161C was preliminarily explored by comparing its structure with the wild type. Notably, r Dca UPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.展开更多
The noncontact blade tip timing(BTT)measurement has been an attractive technology for blade health monitoring(BHM).However,the severe undersampled BTT signal causes a significant challenge for blade vibration paramete...The noncontact blade tip timing(BTT)measurement has been an attractive technology for blade health monitoring(BHM).However,the severe undersampled BTT signal causes a significant challenge for blade vibration parameter identification and fault feature extraction.This study proposes a novel method based on the minimum variance distortionless response(MVDR)of the direction of arrival(DoA)estimation for blade natural frequency estimation from the non-uniformly undersampled BTT signals.First,based on the similarity between the general data acquisition model for BTT and the antenna array model in DoA estimation,the circumferentially arranged probes on the casing can be regarded as a non-uniform linear array.Thus,BTT signal reconstruction is converted into the DoA estimation problem of the non-uniform linear array signal.Second,MVDR is employed to address the severe undersampling issue and recover the BTT undersampled signal.In particular,spatial smoothing is innovatively utilized to enhance the estimation of covariance matrix of the BTT signal to avoid ill-condition or singularity,while improving efficiency and robustness.Lastly,numerical simulation and experimental testing are employed to verify the validity of the proposed method.Monte Carlo simulation results suggest that the proposed method behaves better than conventional methods,especially under a lower signal-to-noise ratio condition.Experimental results indicate that the proposed method can effectively overcome the severe undersampling problem of BTT signal induced by physical limitations,and has a strong potential in the field of BHM.展开更多
基金supported by the National Natural Science Foundation of China (No.32001633)the Key Program of Natural Science Foundation of China (No.31930084)Guangzhou Science and technology planning project (No.202102020370)。
文摘Unspecific peroxygenases(UPOs, EC 1.11.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without cofactors. However, the enhancement of the H_(2)O_(2) tolerance of UPOs is always challenging. In this study, the A161C mutant of r Dca UPO,which originates from Daldinia caldariorum, was found to be highly H_(2)O_(2)-resistant. Compared with the wild type, the mutant r Dca UPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H_(2)O_(2). The crystal structure analysis at 1.47 A showed that r Dca UPOA161C exhibited 10 α-helixes(cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two conserved domains near heme formed an active site with the catalytic PCP and EHD regions(Glu86, His87, Asp88 residues). The H_(2)O_(2) tolerance of r Dca UPO-A161C was preliminarily explored by comparing its structure with the wild type. Notably, r Dca UPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.
基金the National Natural Science Foundation of China(Grant Nos.52105117 and 51875433)the Funds for Distinguished Young Talent of Shaanxi Province,China(Grant No.2019JC-04).
文摘The noncontact blade tip timing(BTT)measurement has been an attractive technology for blade health monitoring(BHM).However,the severe undersampled BTT signal causes a significant challenge for blade vibration parameter identification and fault feature extraction.This study proposes a novel method based on the minimum variance distortionless response(MVDR)of the direction of arrival(DoA)estimation for blade natural frequency estimation from the non-uniformly undersampled BTT signals.First,based on the similarity between the general data acquisition model for BTT and the antenna array model in DoA estimation,the circumferentially arranged probes on the casing can be regarded as a non-uniform linear array.Thus,BTT signal reconstruction is converted into the DoA estimation problem of the non-uniform linear array signal.Second,MVDR is employed to address the severe undersampling issue and recover the BTT undersampled signal.In particular,spatial smoothing is innovatively utilized to enhance the estimation of covariance matrix of the BTT signal to avoid ill-condition or singularity,while improving efficiency and robustness.Lastly,numerical simulation and experimental testing are employed to verify the validity of the proposed method.Monte Carlo simulation results suggest that the proposed method behaves better than conventional methods,especially under a lower signal-to-noise ratio condition.Experimental results indicate that the proposed method can effectively overcome the severe undersampling problem of BTT signal induced by physical limitations,and has a strong potential in the field of BHM.
