A provenance trial of Michelia chapensis Dandy in the south of China was carried out in three sites to assess yield and stability analyses. The trials were conducted as randomized, complete block designs with four rep...A provenance trial of Michelia chapensis Dandy in the south of China was carried out in three sites to assess yield and stability analyses. The trials were conducted as randomized, complete block designs with four replications at each site. Additive main effects and multiplicative interaction（AMMI） and genotype main effect and genotype 9 environment interaction（GGE） were employed in the evaluation of provenances; AMMI analyses showed that significant genotype 9 environment interaction effects（P ／ 0.05） existed between provenances, capturing 15.11 % of the total sum of squares. The AMMI stability value revealed that provenance 2 was stable, but had low yield. GGE-biplot models showed that provenances 7, 8, 10, and 11 were more stable and highyielding. The GGE results also confirmed that among the three sites, site Jiu Qu Shui was the most ideal environment for representation and discrimination.展开更多
Chinese fir（Cunninghamia lanceolata（Lamb.）Hook） is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism（SRAP） primer...Chinese fir（Cunninghamia lanceolata（Lamb.）Hook） is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism（SRAP） primer screening assay with a total of 594 primer combinations,using 22 forward and 27 reverse primers on four representative Chinese fir genotypes. The obtained results indicated that Chinese fir genomic DNA has a notable amplification bias on the employed forward or reverse primer nucleotides（30selection bases）. Out of the tested primer sets, 35 primer combinations with clearly distinguished bands, stable amplification, and rich polymorphism were selected and identified as optimal primer sets. These optimal primer pairs gave a total of 379 scorable bands,including 265 polymorphic bands, with an average of 10.8bands and 7.6 polymorphic bands per primer combination.The produced band number for each optimal primer set ranged from 7 to 14 with a percentage of polymorphic bands spanning from 33.3 to 100.0 %. These primer combinations could facilitate the next SRAP analysis assays in Chinese fir.展开更多
基金supported by Guangdong Forestry Science and Technology Creative Special(2015KJCX003)
文摘A provenance trial of Michelia chapensis Dandy in the south of China was carried out in three sites to assess yield and stability analyses. The trials were conducted as randomized, complete block designs with four replications at each site. Additive main effects and multiplicative interaction（AMMI） and genotype main effect and genotype 9 environment interaction（GGE） were employed in the evaluation of provenances; AMMI analyses showed that significant genotype 9 environment interaction effects（P ／ 0.05） existed between provenances, capturing 15.11 % of the total sum of squares. The AMMI stability value revealed that provenance 2 was stable, but had low yield. GGE-biplot models showed that provenances 7, 8, 10, and 11 were more stable and highyielding. The GGE results also confirmed that among the three sites, site Jiu Qu Shui was the most ideal environment for representation and discrimination.
基金supported by the National Natural Sciences Foundation of China (No. 31200506)the Special Fund for Forest Scientific Research in the Public Welfare (No. 201404127)
文摘Chinese fir（Cunninghamia lanceolata（Lamb.）Hook） is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism（SRAP） primer screening assay with a total of 594 primer combinations,using 22 forward and 27 reverse primers on four representative Chinese fir genotypes. The obtained results indicated that Chinese fir genomic DNA has a notable amplification bias on the employed forward or reverse primer nucleotides（30selection bases）. Out of the tested primer sets, 35 primer combinations with clearly distinguished bands, stable amplification, and rich polymorphism were selected and identified as optimal primer sets. These optimal primer pairs gave a total of 379 scorable bands,including 265 polymorphic bands, with an average of 10.8bands and 7.6 polymorphic bands per primer combination.The produced band number for each optimal primer set ranged from 7 to 14 with a percentage of polymorphic bands spanning from 33.3 to 100.0 %. These primer combinations could facilitate the next SRAP analysis assays in Chinese fir.
