Objective To provide useful information for selecting the most appropriate peripheral nerve injury model for different research purposes in nerve injury and repair studies,and to compare nerve regeneration capacity an...Objective To provide useful information for selecting the most appropriate peripheral nerve injury model for different research purposes in nerve injury and repair studies,and to compare nerve regeneration capacity and characteristics between them.Methods Sixty adult SD rats were randomly divided into two groups and underwent crush injury alone(group A,n=30)or transection injury followed by surgical repair(group B,n=30)of the right hind paw.Each group was subjected to the CatWalk test,gastrocnemius muscle evaluation,pain threshold measurement,electrophysiological examination,retrograde neuronal labeling,and quantification of nerve regeneration before and 7,14,21,and 28 days after injury.Results Gait analysis showed that the recovery speed in group A was significantly faster than that in group B at 14 days.At 21 days,the compound muscle action potential of the gastrocnemius muscle in group A was significantly higher than that in group B,and the number of labeled motor neurons in group B was lower than that in group A.The number of new myelin sheaths and the g-ratio were higher in group A than in group B.There was a 7-day time difference in the regeneration rate between the two injury groups.Conclusion The regeneration of nerve fibers was rapid after crush nerve injury,whereas the transection injury was relatively slow,which provides some ideas for the selection of clinical research models.展开更多
Objective Hypoglossal nerve-facial nerve‘side’-to-side neurorrhaphy is a new method for the treatment of potential incomplete facial paralysis after acoustic neuroma.However,there are differences in postoperative ou...Objective Hypoglossal nerve-facial nerve‘side’-to-side neurorrhaphy is a new method for the treatment of potential incomplete facial paralysis after acoustic neuroma.However,there are differences in postoperative outcomes among patients.This study analysed preoperative factors that may influence the treatment outcomes of neurorrhaphy.Methods We performed a retrospective study of 53 patients who were treated by neurorrhaphy for facial paralysis after acoustic neuroma resection.After a one-year follow-up period,the patients were divided into two groups according to facial functional outcome:better recovery or ordinary recovery.We analysed the following factors:gender,age,tumour size,and characteristics,tumour adhesion to the facial nerve,the duration of facial paralysis(DFP)and F wave appearance prior to neurorrhaphy(F wave).Results Univariate analysis showed significant differences between the two groups in DFP(P=0.0002),tumour adhesion to the facial nerve(P=0.0079)and F waves(P=0.0048).Logistic regression analysis of these factors also showed statistical significance with P values of 0.042 for the DFP,0.043 for F waves,and 0.031 for tumour adhesion to the facial nerve.Conclusions Tumour adhesion to the facial nerve,F waves appearance and DFP prior to neurorrhaphy are the predominant factors that influence treatment outcomes.展开更多
基金supported by the National Natural Science Foundation of China:Investigation on central cortical functional compensation and re-organization after hypoglossal-facial neurorrhaphy for facial nerve injury using repetitive transcranial magnetic stimulation combined with biofeedback peripheral electrical stimulation[Grant No.82171364]the Beijing Municipal Health Commission:Study on repair mechanism of nervous system injury[Grant No.PXM2020_026280_000002].
文摘Objective To provide useful information for selecting the most appropriate peripheral nerve injury model for different research purposes in nerve injury and repair studies,and to compare nerve regeneration capacity and characteristics between them.Methods Sixty adult SD rats were randomly divided into two groups and underwent crush injury alone(group A,n=30)or transection injury followed by surgical repair(group B,n=30)of the right hind paw.Each group was subjected to the CatWalk test,gastrocnemius muscle evaluation,pain threshold measurement,electrophysiological examination,retrograde neuronal labeling,and quantification of nerve regeneration before and 7,14,21,and 28 days after injury.Results Gait analysis showed that the recovery speed in group A was significantly faster than that in group B at 14 days.At 21 days,the compound muscle action potential of the gastrocnemius muscle in group A was significantly higher than that in group B,and the number of labeled motor neurons in group B was lower than that in group A.The number of new myelin sheaths and the g-ratio were higher in group A than in group B.There was a 7-day time difference in the regeneration rate between the two injury groups.Conclusion The regeneration of nerve fibers was rapid after crush nerve injury,whereas the transection injury was relatively slow,which provides some ideas for the selection of clinical research models.
基金supported by grants from the National Natural Science Foundation of China[No.81471239].
文摘Objective Hypoglossal nerve-facial nerve‘side’-to-side neurorrhaphy is a new method for the treatment of potential incomplete facial paralysis after acoustic neuroma.However,there are differences in postoperative outcomes among patients.This study analysed preoperative factors that may influence the treatment outcomes of neurorrhaphy.Methods We performed a retrospective study of 53 patients who were treated by neurorrhaphy for facial paralysis after acoustic neuroma resection.After a one-year follow-up period,the patients were divided into two groups according to facial functional outcome:better recovery or ordinary recovery.We analysed the following factors:gender,age,tumour size,and characteristics,tumour adhesion to the facial nerve,the duration of facial paralysis(DFP)and F wave appearance prior to neurorrhaphy(F wave).Results Univariate analysis showed significant differences between the two groups in DFP(P=0.0002),tumour adhesion to the facial nerve(P=0.0079)and F waves(P=0.0048).Logistic regression analysis of these factors also showed statistical significance with P values of 0.042 for the DFP,0.043 for F waves,and 0.031 for tumour adhesion to the facial nerve.Conclusions Tumour adhesion to the facial nerve,F waves appearance and DFP prior to neurorrhaphy are the predominant factors that influence treatment outcomes.
