Background:Puerarin(Pue)has been reported to be a natural active ingredient with multiple antifibrotic properties.This work aimed at exploring the function of Pue in oral submucousfibrosis(OSF)treatment.Methods:Human or...Background:Puerarin(Pue)has been reported to be a natural active ingredient with multiple antifibrotic properties.This work aimed at exploring the function of Pue in oral submucousfibrosis(OSF)treatment.Methods:Human oral mucosafibroblasts(hOMF)were induced with transforming growth factor beta1(TGF-β1)and intervened with Pue.Expressions offibrosis-related markers were analyzed by Western blot and IF staining.Cell viability was characterized by the CCK-8 assay.Expressions of miR-30 family members were quantified by qRT-PCR.The correlation betweenfibroblast activation protein(FAP)and miR-30 family expression was evaluated by the Pearson correlation coefficient.Bioinformatics prediction and dual-luciferase reporter assay were employed to verify the regulation between FAP and miR-30b-5p.The specific mechanism of Pue on OSF was explored through the promotion or inhibition of miR-30b-5p.Results:After induction by TGF-β1,hOMF showed upregulated Collagen I,Collagen III,and FAP expressions,while miR-30 family expression was downregulated with miR-30b-5p being the most significant.Pue intervention inhibited the excessive proliferation of TGF-β1-induced hOMF,downregulated FAP,collagen type 3(COL3A1),collagen type 1(COL1A1),matrix metalloproteinase 1(MMP1),and matrix metalloproteinase 3(MMP3)expressions,and restored miR-30 family expression.Bioinformatics prediction and dual-luciferase reporter assay revealed that miR-30b-5p selectively inhibited FAP expression.Mechanistically,miR-30b-5p mimic suppressed the excessive proliferation of TGF-β1-induced hOMF and declinedfibrosis levels.Pue intervention significantly reversed the promotion of TGF-β1-induced OSF by miR-30b-5p inhibition.Conclusion:Pue mediated miR-30b-5p targeting FAP against OSF,which provided a theoretical basis for the pathogenesis research and Pue application in OSF.展开更多
目的:编制新生儿重症监护室(neonatal intensive care unit,NICU)早产儿医用粘胶相关性皮肤损伤(medical adhesive related skin injury,MARSI)风险评估量表。方法:通过查阅相关文献并结合专家访谈,运用德尔菲法对20名专家进行函询,经过...目的:编制新生儿重症监护室(neonatal intensive care unit,NICU)早产儿医用粘胶相关性皮肤损伤(medical adhesive related skin injury,MARSI)风险评估量表。方法:通过查阅相关文献并结合专家访谈,运用德尔菲法对20名专家进行函询,经过2轮函询后确定量表维度和条目,研制NICU早产儿MARSI风险评估量表。结果:函询中专家积极性高,2轮问卷有效回收率均为100%,权威系数分别为0.845,0.885,均>0.7,可信度高。2轮专家函询一级及二级指标的肯德尔和谐系数为0.417~0.579(P<0.01),函询结果可靠。经过2轮专家函询,本研究确立了4个一级指标(一般生理状况、皮肤状况、粘胶剂、治疗因素)、10个二级指标(胎龄、出生体重、出生日龄、干燥程度、潮湿程度、水肿程度、粘胶剂类型、粘贴时间、营养摄取方式、蓝光治疗时间)。结论:通过德尔菲法构建了一个规范、系统的NICU早产儿MARSI风险评估量表,适用于NCU早产儿MARSI风险的评估。展开更多
As a key attribute of soil quality, soil organic matter(SOM) and its different fractions play an important role in regulating soil nutrient cycling and soil properties.This study evaluated the soil carbon(C) and nitro...As a key attribute of soil quality, soil organic matter(SOM) and its different fractions play an important role in regulating soil nutrient cycling and soil properties.This study evaluated the soil carbon(C) and nitrogen(N) concentrations in different SOM fractions(light– and heavy fractions,microbial biomass) under different vegetation types and analyzed their influencing factors in continuous permafrost regions along the Qinghai-Tibet Highway in the North of Kunlun Mountains, China.Soil samples were collected in pits under four vegetation types — Alpine swamp meadow(ASM), Alpine meadow(AM), Alpine steppe(AS) and Alpine desert(AD) — at the depth of 0-50 cm.The vegetation coverage was the highest at ASM and AM, followed byAS and AD.The results indicated that the concentrations of light fraction carbon(LFC) and nitrogen(LFN), and microbial biomass carbon(MBC)and nitrogen(MBN) decreased as follows: ASM > AM >AS > AD, with the relatively stronger decrease of LFC,whereas the heavy fraction carbon(HFC) and nitrogen(HFN) concentrations were lower in AS soils than in the AD soils.The relatively higher proportions of LFC/SOC and MBC/SOC in the 0-10 cm depth under the ASM soils are mainly resulted from its higher substrate input and soil moisture content.Correlation analysis demonstrated that aboveground biomass, soil moisture content, soil organic carbon(SOC) and total nitrogen(TN) positively correlated to LFC, LFN, HFC, HFN, MBC and MBN, while p H negatively correlated to LFC, LFN, HFC, HFN, MBC and MBN.There was no relationship between active layer thickness and SOM fractions, except for the LFC.Results suggested that vegetation cover, soil moisture content, and SOC and TN concentrations were significantly correlated with the amount and availability of SOM fractions, while permafrost had less impact on SOM fractions in permafrost regions of the central Qinghai–Tibet Plateau.展开更多
Afforestation is conducive to soil carbon(C) sequestration in semi-arid regions. However, little is known about the effects of afforestation on sequestrations of total and labile soil organic carbon(SOC) fractions in ...Afforestation is conducive to soil carbon(C) sequestration in semi-arid regions. However, little is known about the effects of afforestation on sequestrations of total and labile soil organic carbon(SOC) fractions in semi-arid sandy lands. In the present study, we examined the effects of Caragana microphylla Lam. plantations with different ages(12-and 25-year-old) on sequestrations of total SOC as well as labile SOC fractions such as light fraction organic carbon(LFOC) and microbial biomass carbon(MBC). The analyzed samples were taken from soil depths of 0–5 and 5–15 cm under two shrub-related scenarios: under shrubs and between shrubs with moving sand dunes as control sites in the Horqin Sandy Land of northern China. The results showed that the concentrations and storages of total SOC at soil depths of 0–5 and 5–15 cm were higher in 12-and 25-year-old C. microphylla plantations than in moving sand dunes(i.e., control sites), with the highest value observed under shrubs in 25-year-old C. microphylla plantations. Furthermore, the concentrations and storages of LFOC and MBC showed similar patterns with those of total SOC at the same soil depth. The 12-year-old C. microphylla plantations had higher percentages of LFOC concentration to SOC concentration and MBC concentration to SOC concentration than the 25-year-old C. microphylla plantations and moving sand dunes at both soil depths. A significant positive correlation existed among SOC, LFOC, and MBC, implying that restoring the total and labile SOC fractions is possible by afforestation with C. microphylla shrubs in the Horqin Sandy Land. At soil depth of 0–15 cm, the accumulation rate of total SOC under shrubs was higher in young C. microphylla plantations(18.53 g C/(m^2·a); 0–12 years) than in old C. microphylla plantations(16.24 g C/(m^2·a); 12–25 years), and the accumulation rates of LFOC and MBC under shrubs and between shrubs were also higher in young C. microphylla plantations than in old C. microphylla plantations. It can be concluded that the establishment of C. microphylla in the Horqin Sandy Land may be a good mitigation strategy for SOC sequestration in the surface soils.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.81874496,82374530)the Clinical Medical Technology Innovation Guide Project of Hunan Province(No.2020SK53206)+3 种基金the Natural Science Foundation of Hunan Province(No.2021JJ70062)the Changsha Natural Science Foundation Project(No.kq2014019)the Health Special Fund Research Project of Hunan Province(No.B2020-07)the Clinical Pharmaceutical Research Fund of Hunan Medical Association(No.B202012).
文摘Background:Puerarin(Pue)has been reported to be a natural active ingredient with multiple antifibrotic properties.This work aimed at exploring the function of Pue in oral submucousfibrosis(OSF)treatment.Methods:Human oral mucosafibroblasts(hOMF)were induced with transforming growth factor beta1(TGF-β1)and intervened with Pue.Expressions offibrosis-related markers were analyzed by Western blot and IF staining.Cell viability was characterized by the CCK-8 assay.Expressions of miR-30 family members were quantified by qRT-PCR.The correlation betweenfibroblast activation protein(FAP)and miR-30 family expression was evaluated by the Pearson correlation coefficient.Bioinformatics prediction and dual-luciferase reporter assay were employed to verify the regulation between FAP and miR-30b-5p.The specific mechanism of Pue on OSF was explored through the promotion or inhibition of miR-30b-5p.Results:After induction by TGF-β1,hOMF showed upregulated Collagen I,Collagen III,and FAP expressions,while miR-30 family expression was downregulated with miR-30b-5p being the most significant.Pue intervention inhibited the excessive proliferation of TGF-β1-induced hOMF,downregulated FAP,collagen type 3(COL3A1),collagen type 1(COL1A1),matrix metalloproteinase 1(MMP1),and matrix metalloproteinase 3(MMP3)expressions,and restored miR-30 family expression.Bioinformatics prediction and dual-luciferase reporter assay revealed that miR-30b-5p selectively inhibited FAP expression.Mechanistically,miR-30b-5p mimic suppressed the excessive proliferation of TGF-β1-induced hOMF and declinedfibrosis levels.Pue intervention significantly reversed the promotion of TGF-β1-induced OSF by miR-30b-5p inhibition.Conclusion:Pue mediated miR-30b-5p targeting FAP against OSF,which provided a theoretical basis for the pathogenesis research and Pue application in OSF.
文摘目的:编制新生儿重症监护室(neonatal intensive care unit,NICU)早产儿医用粘胶相关性皮肤损伤(medical adhesive related skin injury,MARSI)风险评估量表。方法:通过查阅相关文献并结合专家访谈,运用德尔菲法对20名专家进行函询,经过2轮函询后确定量表维度和条目,研制NICU早产儿MARSI风险评估量表。结果:函询中专家积极性高,2轮问卷有效回收率均为100%,权威系数分别为0.845,0.885,均>0.7,可信度高。2轮专家函询一级及二级指标的肯德尔和谐系数为0.417~0.579(P<0.01),函询结果可靠。经过2轮专家函询,本研究确立了4个一级指标(一般生理状况、皮肤状况、粘胶剂、治疗因素)、10个二级指标(胎龄、出生体重、出生日龄、干燥程度、潮湿程度、水肿程度、粘胶剂类型、粘贴时间、营养摄取方式、蓝光治疗时间)。结论:通过德尔菲法构建了一个规范、系统的NICU早产儿MARSI风险评估量表,适用于NCU早产儿MARSI风险的评估。
基金financially supported by the National Major Scientific Project of China"Cryospheric Change and Impacts Research"program"Research of permafrost hydrothermal process and its response to climate change"(Grant No.2013CBA01803)supported in part by Science Fund for Creative Research Groups of the National Natural Science Foundation of China(Grant No.41121001)+2 种基金the National Natural Science Foundation of China(Grant No.41101055)the West Light Foundation of the Chinese Academy of Sciencesthe Foundation for Excellent Youth Scholars of Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences
文摘As a key attribute of soil quality, soil organic matter(SOM) and its different fractions play an important role in regulating soil nutrient cycling and soil properties.This study evaluated the soil carbon(C) and nitrogen(N) concentrations in different SOM fractions(light– and heavy fractions,microbial biomass) under different vegetation types and analyzed their influencing factors in continuous permafrost regions along the Qinghai-Tibet Highway in the North of Kunlun Mountains, China.Soil samples were collected in pits under four vegetation types — Alpine swamp meadow(ASM), Alpine meadow(AM), Alpine steppe(AS) and Alpine desert(AD) — at the depth of 0-50 cm.The vegetation coverage was the highest at ASM and AM, followed byAS and AD.The results indicated that the concentrations of light fraction carbon(LFC) and nitrogen(LFN), and microbial biomass carbon(MBC)and nitrogen(MBN) decreased as follows: ASM > AM >AS > AD, with the relatively stronger decrease of LFC,whereas the heavy fraction carbon(HFC) and nitrogen(HFN) concentrations were lower in AS soils than in the AD soils.The relatively higher proportions of LFC/SOC and MBC/SOC in the 0-10 cm depth under the ASM soils are mainly resulted from its higher substrate input and soil moisture content.Correlation analysis demonstrated that aboveground biomass, soil moisture content, soil organic carbon(SOC) and total nitrogen(TN) positively correlated to LFC, LFN, HFC, HFN, MBC and MBN, while p H negatively correlated to LFC, LFN, HFC, HFN, MBC and MBN.There was no relationship between active layer thickness and SOM fractions, except for the LFC.Results suggested that vegetation cover, soil moisture content, and SOC and TN concentrations were significantly correlated with the amount and availability of SOM fractions, while permafrost had less impact on SOM fractions in permafrost regions of the central Qinghai–Tibet Plateau.
基金funded by the National Natural Science Foundation of China (31640012, 41271007, 31660232)the One Hundred Person Project of the Chinese Academy of Sciences (Y551821)+1 种基金the Opening Foundation of the State Key Laboratory Breeding Base of DesertificationAeolian Sand Disaster Combating, Gansu Desert Control Research Institute (GSDC201505)
文摘Afforestation is conducive to soil carbon(C) sequestration in semi-arid regions. However, little is known about the effects of afforestation on sequestrations of total and labile soil organic carbon(SOC) fractions in semi-arid sandy lands. In the present study, we examined the effects of Caragana microphylla Lam. plantations with different ages(12-and 25-year-old) on sequestrations of total SOC as well as labile SOC fractions such as light fraction organic carbon(LFOC) and microbial biomass carbon(MBC). The analyzed samples were taken from soil depths of 0–5 and 5–15 cm under two shrub-related scenarios: under shrubs and between shrubs with moving sand dunes as control sites in the Horqin Sandy Land of northern China. The results showed that the concentrations and storages of total SOC at soil depths of 0–5 and 5–15 cm were higher in 12-and 25-year-old C. microphylla plantations than in moving sand dunes(i.e., control sites), with the highest value observed under shrubs in 25-year-old C. microphylla plantations. Furthermore, the concentrations and storages of LFOC and MBC showed similar patterns with those of total SOC at the same soil depth. The 12-year-old C. microphylla plantations had higher percentages of LFOC concentration to SOC concentration and MBC concentration to SOC concentration than the 25-year-old C. microphylla plantations and moving sand dunes at both soil depths. A significant positive correlation existed among SOC, LFOC, and MBC, implying that restoring the total and labile SOC fractions is possible by afforestation with C. microphylla shrubs in the Horqin Sandy Land. At soil depth of 0–15 cm, the accumulation rate of total SOC under shrubs was higher in young C. microphylla plantations(18.53 g C/(m^2·a); 0–12 years) than in old C. microphylla plantations(16.24 g C/(m^2·a); 12–25 years), and the accumulation rates of LFOC and MBC under shrubs and between shrubs were also higher in young C. microphylla plantations than in old C. microphylla plantations. It can be concluded that the establishment of C. microphylla in the Horqin Sandy Land may be a good mitigation strategy for SOC sequestration in the surface soils.
