This study was to investigate the protective effect of paeoniflorin(PF)on hydrogen peroxide-induced injury.Firstly,“SMILES”of PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Ta...This study was to investigate the protective effect of paeoniflorin(PF)on hydrogen peroxide-induced injury.Firstly,“SMILES”of PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets.Injury-related molecules were obtained from GeenCards database,and the predicted targets of PF for injury treatment were selected by Wayne diagram.For mechanism analysis,the protein-protein interactions were constructed by String,and the KEGG analysis was conducted in Webgestalt.Then,cell viability and cytotoxicity assay were established by CCK8 assay.Also,the experimental cells were allocated to control,model(200μmol·L^(−1) H_(2)O_(2)),SB20358010μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+SB20358010μmol·L^(−1)),PF 50μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 50μmol·L^(−1)),and PF 100μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 100μmol·L^(−1))groups.We measured the intracellular ROS,Hoechst 33258 staining,cell apoptosis,the levels of Bcl-xl,Bcl-2,Caspase-3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the phosphorylation expression of p38MAPK.There are 96 potential targets that may be associated with PF for injury treatment.Then,we chose the“Inflammatory mediator regulation of TRP channels”pathway for the experimental verification from the first 10 KEGG pathway.In experimental verification,H_(2)O_(2) decreased the cell viability moderately(P<0.05),and 100μmol·L^(−1) PF increased the cell viability significantly(P<0.05).Depending on the difference of intracellular ROS fluorescence intensity,PF inhibited H_(2)O_(2)-induced reactive oxygen species production in Schwann cells.In Hoechst 33258 staining,PF reversed the condensed chromatin and apoptotic nuclei following H_(2)O_(2) treatment.Moreover,Flow cytometry results showed that PF could substantially inhibit H_(2)O_(2) induced apoptosis(P<0.05).Pretreatment with PF obviously reduced the levels of Caspase3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the phosphorylation expression of p38MAPK after H_(2)O_(2) treatment(P<0.05),increased the levels of Bcl-2,and Bcl-xl(P<0.05).PF inhibited Schwann cell injury and apoptosis induced by hydrogen peroxide,which mechanism was linked to the inhibition of phosphorylation of p38MAPK.展开更多
Objective： To investigate the effects of health education based on integrative therapy of Chinese and Western medicine for type 2 diabetes mellitus（T2DM） from the aspects of knowledge, attitude and practice（KAP）,...Objective： To investigate the effects of health education based on integrative therapy of Chinese and Western medicine for type 2 diabetes mellitus（T2DM） from the aspects of knowledge, attitude and practice（KAP）, health-related quality of life（HRQo L）, body mass index（BMI） and glucose control. Methods： Patients were individually randomized into intervention group（receiving integrative education, n=120） and control group（receiving usual education, n=120）. The primary outcome was the changes in glycosylated hemoglobin A1c（HbA1c） levels after 3, 6, 9 and 12 months from baseline. Hierarchical linear models（HLMs） were used to assess within-group changes in outcomes over time and between-group differences in patterns of change. Secondary outcomes were KAP scores, HRQo L scores and BMI after 6 and 12 months, paired-sample t test was used to assess within-group changes in outcomes in 6 and 12 months, independent-sample t test was used to assess between-group differences in patterns of change. Results： HbA1c decreased statistically from baseline to 3 months, from 3 to 6 months, from 6 to 9 months and from 9 to 12 months in the intervention group（all P〈0.01）; and decreased significantly from baseline to 3 months, and from 3 to 6 months in the control group（P〈0.01）. There were significant between-group differences from baseline to 3 months（P=0.044）, from 6 to 9 months（P〈0.01） and from 9 to 12 months（P〈0.01）. Significant improvements in the intervention group along with significant between-group differences were found in KAP and HRQo L scores respectively（all P〈0.05）. The number in the intervention group of normal weight increased from 56 at baseline to 81（6 months）, 94（12 months）, the number in the control group were 63（baseline）, 69（6 months）, 70（12 months）, the χ~2 of hierarchical analysis of BMI were 6.93（P=0.075）, 10.31（P=0.016）, 15.53（P〈0.01）, respectively. Conclusion： Health education based on integrative therapy of Chinese and Western medicine is beneficial to the control of T2DM and should be recommended for T2DM.展开更多
基金the National Natural Science Foundation of China(No.81874404).
文摘This study was to investigate the protective effect of paeoniflorin(PF)on hydrogen peroxide-induced injury.Firstly,“SMILES”of PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets.Injury-related molecules were obtained from GeenCards database,and the predicted targets of PF for injury treatment were selected by Wayne diagram.For mechanism analysis,the protein-protein interactions were constructed by String,and the KEGG analysis was conducted in Webgestalt.Then,cell viability and cytotoxicity assay were established by CCK8 assay.Also,the experimental cells were allocated to control,model(200μmol·L^(−1) H_(2)O_(2)),SB20358010μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+SB20358010μmol·L^(−1)),PF 50μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 50μmol·L^(−1)),and PF 100μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 100μmol·L^(−1))groups.We measured the intracellular ROS,Hoechst 33258 staining,cell apoptosis,the levels of Bcl-xl,Bcl-2,Caspase-3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the phosphorylation expression of p38MAPK.There are 96 potential targets that may be associated with PF for injury treatment.Then,we chose the“Inflammatory mediator regulation of TRP channels”pathway for the experimental verification from the first 10 KEGG pathway.In experimental verification,H_(2)O_(2) decreased the cell viability moderately(P<0.05),and 100μmol·L^(−1) PF increased the cell viability significantly(P<0.05).Depending on the difference of intracellular ROS fluorescence intensity,PF inhibited H_(2)O_(2)-induced reactive oxygen species production in Schwann cells.In Hoechst 33258 staining,PF reversed the condensed chromatin and apoptotic nuclei following H_(2)O_(2) treatment.Moreover,Flow cytometry results showed that PF could substantially inhibit H_(2)O_(2) induced apoptosis(P<0.05).Pretreatment with PF obviously reduced the levels of Caspase3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the phosphorylation expression of p38MAPK after H_(2)O_(2) treatment(P<0.05),increased the levels of Bcl-2,and Bcl-xl(P<0.05).PF inhibited Schwann cell injury and apoptosis induced by hydrogen peroxide,which mechanism was linked to the inhibition of phosphorylation of p38MAPK.
基金Supported by the National Natural Science Foundation of China(No.30873256)
文摘Objective： To investigate the effects of health education based on integrative therapy of Chinese and Western medicine for type 2 diabetes mellitus（T2DM） from the aspects of knowledge, attitude and practice（KAP）, health-related quality of life（HRQo L）, body mass index（BMI） and glucose control. Methods： Patients were individually randomized into intervention group（receiving integrative education, n=120） and control group（receiving usual education, n=120）. The primary outcome was the changes in glycosylated hemoglobin A1c（HbA1c） levels after 3, 6, 9 and 12 months from baseline. Hierarchical linear models（HLMs） were used to assess within-group changes in outcomes over time and between-group differences in patterns of change. Secondary outcomes were KAP scores, HRQo L scores and BMI after 6 and 12 months, paired-sample t test was used to assess within-group changes in outcomes in 6 and 12 months, independent-sample t test was used to assess between-group differences in patterns of change. Results： HbA1c decreased statistically from baseline to 3 months, from 3 to 6 months, from 6 to 9 months and from 9 to 12 months in the intervention group（all P〈0.01）; and decreased significantly from baseline to 3 months, and from 3 to 6 months in the control group（P〈0.01）. There were significant between-group differences from baseline to 3 months（P=0.044）, from 6 to 9 months（P〈0.01） and from 9 to 12 months（P〈0.01）. Significant improvements in the intervention group along with significant between-group differences were found in KAP and HRQo L scores respectively（all P〈0.05）. The number in the intervention group of normal weight increased from 56 at baseline to 81（6 months）, 94（12 months）, the number in the control group were 63（baseline）, 69（6 months）, 70（12 months）, the χ~2 of hierarchical analysis of BMI were 6.93（P=0.075）, 10.31（P=0.016）, 15.53（P〈0.01）, respectively. Conclusion： Health education based on integrative therapy of Chinese and Western medicine is beneficial to the control of T2DM and should be recommended for T2DM.
