Objective:Esophageal carcinoma is the eighth most common malignant tumor and ranked the sixth cause of death in the worldwide careinoma.To find out effective serum markers for early diagnosis and targeted treatment is...Objective:Esophageal carcinoma is the eighth most common malignant tumor and ranked the sixth cause of death in the worldwide careinoma.To find out effective serum markers for early diagnosis and targeted treatment is important to improve esophageal carcinoma survival rate.The aim of this study was to screen differ-entially expressed serum protein profiling in esophageal squamous cell careinoma(ESCC)patients with differ-ent Chinese medicine syndrome and establish ESCC syndrome diagnosis model,to explore the signifcance of ESCC serum differential protein in traditional Chinese medicine(TCM)syndrome diagnosis and provide exper-imental and theoretical basis for the diagnosis,clinical treatment of ESCC.Methods:Serum specimens of 80 ES-CC patients with different syndrome and 20 healthy people were selected according to distribution of their sex and age.Magnetic beads based weak cation exchange combined w ith matrix-assist ed laser desorption/ionization time-of-flight mass spectrometry prot eomic technology and Autoflex Analysis and ClinProTools software were applied to screen differentially expressed serum protein profiling.Then establishing each syndrome group diag-nosis model and verifying with new samples to evaluate the diagnosis value of each syndrome group diagnosis model.Results:18 significantly differential expression protein peaks were screened between the whole ESCC patients sera and the healthy control group.Compared with normal control group,among which,ten were signif-cant down-regulation with m/z values of4210,4267,4054,4248,2106,4091,2991,5337,3952,822,and eight were significant up-regulation with m/z values of 2900,2883,1467,1617,2862,2600,895,811.There were 16,12,11,15 differentially expressed protein peaks in phlegm and qi stagnat ion syndrome(Group P),fluid insufficiency and heat agglomeration syndrome(Group F),blood stasis syndrome(Group B),qi and yang deficiency syndrome(Group Q),respectively.Protein with m/z of4210,4091,4054 and 5337 presen-ted obviously low expression in all syndrome groups,while protein with m/z of2883,1617 presented obvious-y high expression.Protein with m/z of 4644 is an obvious and significative biomarker in the comparison of dif-ferent syndrome of ESCC.Establishing ESCC each syndrome serum different ial protein profiling diagnosis mod-el showed that the specificity and veracity in four syndrome groups were all≥80.00%.Conclusion:Our study achieved differentially expressed protein profiling in ESCC patients with different TCM syndromes,established relatively high sensitivity and specificity of different syndrome of ESCC serum differential protein profiling diag-nosis model,which implied the screened syndrome related serum differential expression protein in TCM syn-drome differentiation and diagnosis had great clinical meaning,could be the assistant diagnosis biomarker of ESCC.展开更多
Objective:To explore the molecular mechanism of the prescription for the syndrome of cold-damp-ness obstructing the lung in the treatment of corona virus disease 2019(COVID-19).Methods:The medicinals for the treatment...Objective:To explore the molecular mechanism of the prescription for the syndrome of cold-damp-ness obstructing the lung in the treatment of corona virus disease 2019(COVID-19).Methods:The medicinals for the treatment of the syndrome of cold-dampness obstructing the lung,such as Cangzhu(Rhizoma Atractylo-dis),Chenpi(Pericarpium Gitri Reticulatae),Houpo(Cortex Magnoliae Officinalis),Huoxiang(Herba Agastachis),Caoguo(Fructus Tsaoko),Mahuang(Herba Ephedrae),Qianghuo(Rhizoma et Radix Notoptery-gi),Shengjiang(Rhizoma Zingiberis Recens),Binlang(Semen Arecae)in the Diagnosis and Treatment Pro-gram of COVID-19(Trial Version 6)were taken as research subjects,and the combination of these nine me-dicinals can be called Hanshi Zufei Fang(寒湿阻肺方,HSZFF).The active components and targets of each single Chinese materia medica was screened and obtained through the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database.The target information related to COVID-19 was retrieved through the Gene-Cards disease-related target database.The medicinal prediction targets were mapped to the disease target to ob-tain the intersection targets.The DAVID database was applied to perform gene ontology(GO)enrichment anal-ysis and kyoto encyclopedia of genes and genomes(KEGG)pathway analysis on the targets;GraphPad Prism 5.0 software was applied to plot the biological process(BP)of GO enrichment analysis,cellular component(CC),molecular function(MF)histograms;OmicShare online software was applied to make KEGG advanced bubble chart;Cytoscape software was applied to visualize the interaction with the targets and Chinese materia medica-components-targets results.Results:Totally 56 key active components of 9 Chinese materia medica for cold-dampness obstructing lung syndrome were screened,and 55 targets were obtained.The results of GO and KEGG enrichment analysis showed that the compound prescription mainly regulated the body's immune re-sponse and reduced inflammation by regulating such signaling pathways of inflammatory response and immune regulation as TNF signaling pathway,HIF-1 signaling pathway,Toll-like receptor signaling pathway,infuenza A signaling pathway,T cell receptor signaling pathway.Conclusion:HSZFF can eliminate infl ammation and inhibit virus by regulating immune inflammatory factors closely related to the occurrence and development of diseases through multi-component and multi-target.展开更多
目前,泛癌图谱计划已实现从单个肿瘤类型的分子描述到跨越不同肿瘤类型的分子分析,在癌症易感性变异、致癌通路共现与互斥、生物调控网络紊乱和病毒感染对癌症的影响等方面均有不同程度的进展。泛癌计划的研究模式和研究内容是对癌症基...目前,泛癌图谱计划已实现从单个肿瘤类型的分子描述到跨越不同肿瘤类型的分子分析,在癌症易感性变异、致癌通路共现与互斥、生物调控网络紊乱和病毒感染对癌症的影响等方面均有不同程度的进展。泛癌计划的研究模式和研究内容是对癌症基因组图谱(the cancer genome atlas,TCGA)数据的整合和升华,与中医整体观念有一定程度的契合,尤其是与中医先天禀赋、五行理论、六淫外袭等理论有相通之处。但TCGA数据多来自原发肿瘤,而临床患者通常死于复发转移,故需进一步探讨癌前病变、首要病变、转移性肿瘤与药物治疗敏感性或耐药性的关系。展开更多
目的:基于网络药理学与分子对接技术分析当归芍药散治疗卵巢癌(ovarian cancer)的药理机制。方法:结合文献和中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP...目的:基于网络药理学与分子对接技术分析当归芍药散治疗卵巢癌(ovarian cancer)的药理机制。方法:结合文献和中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)对当归芍药散成分和作用靶点进行检索,将药物靶点和Genecards数据库所载卵巢癌靶标进行对比,结果输入STRING数据库,并在Cytoscape3.8.0软件中制作网络图。采用Rstudio软件进行基因本体(gene ontology,GO)功能富集分析、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。利用PyMOL软件对排名前5位的活性成分和核心靶标进行分子对接可视化分析。结果:筛选共得药物活性成分70个,涉及VEGFA、RELA、PTGS2、MMP9、TP53、JUN、PGR等98个作用靶标;GO功能富集分析、KEGG信号通路富集结果显示:当归芍药散治疗卵巢癌主要通过药物反应、RNA聚合酶Ⅱ启动子转录的正调控、雌二醇反应等调节磷酸酰肌醇3-激酶/蛋白激酶B(phosphatidylinositol 3-kinase-Akt signal transduction pathway/protein kinase B,PI3K/Akt)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、血管内皮生长因子(vascular endothelial growth factor,VEGF)等信号通路;分子对接结果表明黄芩素、鞣花酸与靶标蛋白有较好的结合活性。结论:当归芍药散治疗卵巢癌可能与VEGFA、TP53、PTGS2、PGR等作用靶标及调节PI3K-Akt、MAPK、VEGF等信号通路有关,黄芩素、鞣花酸可能是其发挥作用的主要成分。展开更多
To promote the development of Traditional Chinese Medicine (TCM), it is necessary to innovate the traditional prescription. It is feasible to use one or several components to substitute TCM, which can be regarded as a...To promote the development of Traditional Chinese Medicine (TCM), it is necessary to innovate the traditional prescription. It is feasible to use one or several components to substitute TCM, which can be regarded as a process of discarding the dregs and preserving the essential components. In this way, traditional prescription can be converted into various combinations of pharmacological ingredients deriving from several TCMs. Furthermore, some of pharmacological ingredients should be modified to increase their efficacy. It is practical to select the main structural unit with specific substituents having strong pharmacological activity. After the innovation mentioned above, the prescription will evolve into a variety of modified components having distinct pharmacological activity, and this is the novel integration of active ingredients.展开更多
基金We thank for the fun-ding support from the Excellent project of Na-tional Returned Scholars Funds[(2007)170]National Natural Science Foundation of China(30873220,30973698,81550014)。
文摘Objective:Esophageal carcinoma is the eighth most common malignant tumor and ranked the sixth cause of death in the worldwide careinoma.To find out effective serum markers for early diagnosis and targeted treatment is important to improve esophageal carcinoma survival rate.The aim of this study was to screen differ-entially expressed serum protein profiling in esophageal squamous cell careinoma(ESCC)patients with differ-ent Chinese medicine syndrome and establish ESCC syndrome diagnosis model,to explore the signifcance of ESCC serum differential protein in traditional Chinese medicine(TCM)syndrome diagnosis and provide exper-imental and theoretical basis for the diagnosis,clinical treatment of ESCC.Methods:Serum specimens of 80 ES-CC patients with different syndrome and 20 healthy people were selected according to distribution of their sex and age.Magnetic beads based weak cation exchange combined w ith matrix-assist ed laser desorption/ionization time-of-flight mass spectrometry prot eomic technology and Autoflex Analysis and ClinProTools software were applied to screen differentially expressed serum protein profiling.Then establishing each syndrome group diag-nosis model and verifying with new samples to evaluate the diagnosis value of each syndrome group diagnosis model.Results:18 significantly differential expression protein peaks were screened between the whole ESCC patients sera and the healthy control group.Compared with normal control group,among which,ten were signif-cant down-regulation with m/z values of4210,4267,4054,4248,2106,4091,2991,5337,3952,822,and eight were significant up-regulation with m/z values of 2900,2883,1467,1617,2862,2600,895,811.There were 16,12,11,15 differentially expressed protein peaks in phlegm and qi stagnat ion syndrome(Group P),fluid insufficiency and heat agglomeration syndrome(Group F),blood stasis syndrome(Group B),qi and yang deficiency syndrome(Group Q),respectively.Protein with m/z of4210,4091,4054 and 5337 presen-ted obviously low expression in all syndrome groups,while protein with m/z of2883,1617 presented obvious-y high expression.Protein with m/z of 4644 is an obvious and significative biomarker in the comparison of dif-ferent syndrome of ESCC.Establishing ESCC each syndrome serum different ial protein profiling diagnosis mod-el showed that the specificity and veracity in four syndrome groups were all≥80.00%.Conclusion:Our study achieved differentially expressed protein profiling in ESCC patients with different TCM syndromes,established relatively high sensitivity and specificity of different syndrome of ESCC serum differential protein profiling diag-nosis model,which implied the screened syndrome related serum differential expression protein in TCM syn-drome differentiation and diagnosis had great clinical meaning,could be the assistant diagnosis biomarker of ESCC.
基金We thank for the funding support from the National Natural Science Foundation of China(81873285)。
文摘Objective:To explore the molecular mechanism of the prescription for the syndrome of cold-damp-ness obstructing the lung in the treatment of corona virus disease 2019(COVID-19).Methods:The medicinals for the treatment of the syndrome of cold-dampness obstructing the lung,such as Cangzhu(Rhizoma Atractylo-dis),Chenpi(Pericarpium Gitri Reticulatae),Houpo(Cortex Magnoliae Officinalis),Huoxiang(Herba Agastachis),Caoguo(Fructus Tsaoko),Mahuang(Herba Ephedrae),Qianghuo(Rhizoma et Radix Notoptery-gi),Shengjiang(Rhizoma Zingiberis Recens),Binlang(Semen Arecae)in the Diagnosis and Treatment Pro-gram of COVID-19(Trial Version 6)were taken as research subjects,and the combination of these nine me-dicinals can be called Hanshi Zufei Fang(寒湿阻肺方,HSZFF).The active components and targets of each single Chinese materia medica was screened and obtained through the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database.The target information related to COVID-19 was retrieved through the Gene-Cards disease-related target database.The medicinal prediction targets were mapped to the disease target to ob-tain the intersection targets.The DAVID database was applied to perform gene ontology(GO)enrichment anal-ysis and kyoto encyclopedia of genes and genomes(KEGG)pathway analysis on the targets;GraphPad Prism 5.0 software was applied to plot the biological process(BP)of GO enrichment analysis,cellular component(CC),molecular function(MF)histograms;OmicShare online software was applied to make KEGG advanced bubble chart;Cytoscape software was applied to visualize the interaction with the targets and Chinese materia medica-components-targets results.Results:Totally 56 key active components of 9 Chinese materia medica for cold-dampness obstructing lung syndrome were screened,and 55 targets were obtained.The results of GO and KEGG enrichment analysis showed that the compound prescription mainly regulated the body's immune re-sponse and reduced inflammation by regulating such signaling pathways of inflammatory response and immune regulation as TNF signaling pathway,HIF-1 signaling pathway,Toll-like receptor signaling pathway,infuenza A signaling pathway,T cell receptor signaling pathway.Conclusion:HSZFF can eliminate infl ammation and inhibit virus by regulating immune inflammatory factors closely related to the occurrence and development of diseases through multi-component and multi-target.
文摘目前,泛癌图谱计划已实现从单个肿瘤类型的分子描述到跨越不同肿瘤类型的分子分析,在癌症易感性变异、致癌通路共现与互斥、生物调控网络紊乱和病毒感染对癌症的影响等方面均有不同程度的进展。泛癌计划的研究模式和研究内容是对癌症基因组图谱(the cancer genome atlas,TCGA)数据的整合和升华,与中医整体观念有一定程度的契合,尤其是与中医先天禀赋、五行理论、六淫外袭等理论有相通之处。但TCGA数据多来自原发肿瘤,而临床患者通常死于复发转移,故需进一步探讨癌前病变、首要病变、转移性肿瘤与药物治疗敏感性或耐药性的关系。
文摘目的:基于网络药理学与分子对接技术分析当归芍药散治疗卵巢癌(ovarian cancer)的药理机制。方法:结合文献和中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)对当归芍药散成分和作用靶点进行检索,将药物靶点和Genecards数据库所载卵巢癌靶标进行对比,结果输入STRING数据库,并在Cytoscape3.8.0软件中制作网络图。采用Rstudio软件进行基因本体(gene ontology,GO)功能富集分析、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。利用PyMOL软件对排名前5位的活性成分和核心靶标进行分子对接可视化分析。结果:筛选共得药物活性成分70个,涉及VEGFA、RELA、PTGS2、MMP9、TP53、JUN、PGR等98个作用靶标;GO功能富集分析、KEGG信号通路富集结果显示:当归芍药散治疗卵巢癌主要通过药物反应、RNA聚合酶Ⅱ启动子转录的正调控、雌二醇反应等调节磷酸酰肌醇3-激酶/蛋白激酶B(phosphatidylinositol 3-kinase-Akt signal transduction pathway/protein kinase B,PI3K/Akt)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、血管内皮生长因子(vascular endothelial growth factor,VEGF)等信号通路;分子对接结果表明黄芩素、鞣花酸与靶标蛋白有较好的结合活性。结论:当归芍药散治疗卵巢癌可能与VEGFA、TP53、PTGS2、PGR等作用靶标及调节PI3K-Akt、MAPK、VEGF等信号通路有关,黄芩素、鞣花酸可能是其发挥作用的主要成分。
基金Supported by Natural Science Foundation-funded Project:study of velvet antler polypeptides differentiating mesenchymal stem cells toward neurons in vitro(No.81470171)Natural Science Foundation-funded Project:Suxiaopingchuan prescription can therapy model mice of airway remodeling in asthma and reduce the inflammatory secretion of sensitized nci-h292 cells by inhibiting the TGFβ1-SMAD3-SMURF2 signaling pathway(81803915)
文摘To promote the development of Traditional Chinese Medicine (TCM), it is necessary to innovate the traditional prescription. It is feasible to use one or several components to substitute TCM, which can be regarded as a process of discarding the dregs and preserving the essential components. In this way, traditional prescription can be converted into various combinations of pharmacological ingredients deriving from several TCMs. Furthermore, some of pharmacological ingredients should be modified to increase their efficacy. It is practical to select the main structural unit with specific substituents having strong pharmacological activity. After the innovation mentioned above, the prescription will evolve into a variety of modified components having distinct pharmacological activity, and this is the novel integration of active ingredients.
