Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and noris...Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and norisoprenoids in pine board might be introduced into Qingke Baijiu.Thus,the terpenoids and norisoprenoids in Qingke Baijiu were investigated by headspace solid phase microextraction(HS-SPME)combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry(GC×GC-TOFMS).The results showed thatγ-terpinene(0.70-530.72μg/L),α-phellandrene(0.34-256.66μg/L),longicyclene(0-38.55μg/L),α-pinene(1.21-35.54μg/L)and limonene(0.93-23.69μg/L)were the top 5 terpenoids/norisoprenoids in Qingke Baijiu.Pulegone was detected and reported for the first time in Baijiu,and the concentrations in Qingke Baijiu were 0.78μg/L(fresh)and 3.90μg/L(7 years old).According to the principal component analysis(PCA)plot,young,aged,and retail Qingke Baijiu could be differentiated clearly.Fold change(FC)and t-tests analysis indicated thatβ-pinene,γ-terpinene,andα-selinene were the most different terpenoids/norisoprenoids between young and aged Qingke Baijiu,and longifolene was the most different terpenoids/norisoprenoids between base and retail Qingke Baijiu.The terpenoids and norisoprenoids,such as(E)-β-ionone,isoborneol andβ-cyclocitral,could be potential markers indicating the ageing process of Qingke Baijiu.展开更多
The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 w...The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.展开更多
The visual system is vital to human health, and the effects of ionizing radiation on vision have received increasing attention. Using a zebrafish model, this study was designed to investigate the apoptosis in eyes ind...The visual system is vital to human health, and the effects of ionizing radiation on vision have received increasing attention. Using a zebrafish model, this study was designed to investigate the apoptosis in eyes induced by carbonion irradiation. Zebrafish embryos at 12 h post-fertilization (hpf) were irradiated using 12C6+ion beams at doses of 2, 4 and 8 Gy.展开更多
CO is increasingly appreciated as a signal molecule, due to its protective features, such as anti-inflammatoryfunction[1], anti-apoptosis[2]. Carbon monoxide -releasing molecules (CORMs) represent a group of compounds...CO is increasingly appreciated as a signal molecule, due to its protective features, such as anti-inflammatoryfunction[1], anti-apoptosis[2]. Carbon monoxide -releasing molecules (CORMs) represent a group of compounds thatare capable of modulating physiological functions via liberating CO. Among various CORMs synthetized, CORM-3is one of the most promising compounds because it is soluble in water and the half-life to liberate CO in saline was10.6 h, thus imitating the action of CO more closely[3].展开更多
Radioresistance serves as the main contributor to treatment failure,bringing about tumour relapse and metas-tasis.Quiescent cancer cells are therapeutically challenging owing to their resistance to most conventional p...Radioresistance serves as the main contributor to treatment failure,bringing about tumour relapse and metas-tasis.Quiescent cancer cells are therapeutically challenging owing to their resistance to most conventional photon treatments that selectively act on proliferating cells^([1,2]).Heavy-ion radiotherapy(RT)has become an increasingly valid treatment option owing to its advantageous dose profile and radio-biological effects compared to conventional RT^([3]).展开更多
The radioresistance of cancer cells is a major factor leading to radiotherapy failure and poor prognosis in patients.Quiescent cells are considered a major reason for cancer radioresistance due to their larger hypoxic...The radioresistance of cancer cells is a major factor leading to radiotherapy failure and poor prognosis in patients.Quiescent cells are considered a major reason for cancer radioresistance due to their larger hypoxic fraction and stronger repair ability^([1]).Compared with proliferating cancer cells,quiescent cancer cells have lower sensitivity to radiotherapy and chemotherapy,and stronger repair ability^([2,3]).展开更多
Cervical carcinoma is one of the most common and lethal gynecological malignancy in women worldwide,and its mortality rates only rank second to breast cancer.Recently,^12C^6+radiotherapy,as a highly intensive local th...Cervical carcinoma is one of the most common and lethal gynecological malignancy in women worldwide,and its mortality rates only rank second to breast cancer.Recently,^12C^6+radiotherapy,as a highly intensive local therapy.展开更多
Cervical cancer is the second most common malignant tumour threatening women's health.In recent years,heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer,however,radio-resistance and rad...Cervical cancer is the second most common malignant tumour threatening women's health.In recent years,heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer,however,radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer[1,2].展开更多
The inhibitory effects of carbon monoxide(CO),generated by Ru(CO)3Cl-glycinate[CO-releasing molecule(CORM-3)],on cell apoptosis in zebrafish embryos induced by ionizing radiation with different linear energy transfer(...The inhibitory effects of carbon monoxide(CO),generated by Ru(CO)3Cl-glycinate[CO-releasing molecule(CORM-3)],on cell apoptosis in zebrafish embryos induced by ionizing radiation with different linear energy transfer(LET)were studied.Zebrafish embryos at 5 h post-fertilization(hpf)were irradiated with X-ray(low-LET)and carbon-ion(high-LET)with or without pretreatment of CORM-31 h before irradiation.Embryos were divided into four groups:(i)the control group(C),which received no further treatments after rinsed with E3 culture medium;(ii)CORM-3-pretreated group(CORM),which did not receive irradiation after exposed to 10M CORM-3 at 4 hpf;(iii)irradiation(IR)groups,which received X-ray or 12C6+ion irradiation at 5 hpf and(iv)CORM-3-pretreatment+irradiationgroup(CORM+IR),which received 10M CORM-3 treatment 1h before X-ray or 12C6+ion irradiation.The apoptotic signals of living embryos at 24 hpf were detected through staining with the vital dye acridine orange(AO).展开更多
F1Fo ATP synthase,or named ATPase(EC 3.6.1.3)(referred as the same enzyme hereafter)catalyzes the phosphorylation of ADP to ATP by exploiting a the transmembrane proton motive force(Δp)produced by the oxidation of nu...F1Fo ATP synthase,or named ATPase(EC 3.6.1.3)(referred as the same enzyme hereafter)catalyzes the phosphorylation of ADP to ATP by exploiting a the transmembrane proton motive force(Δp)produced by the oxidation of nutrient substrates in mitochondrial respiration in all organisms.However,under peculiar physiological conditions when mitochondrial respiration is impaired which lead to the electrochemical gradient across the inner membrane collapse,the F1Fo ATP synthase switches its catalytic activity from ATP synthesis to ATP hydrolysis for maintaining theΔp.展开更多
All living organisms and ecosystems are permanently exposed to ionizing radiation.Of all the types of ionizing radiation,heavy ions such as 56Fe have the potential to cause the most severe biological effects.We theref...All living organisms and ecosystems are permanently exposed to ionizing radiation.Of all the types of ionizing radiation,heavy ions such as 56Fe have the potential to cause the most severe biological effects.We therefore examined the effects and potential mechanisms of iron ion irradiation on the induction of apoptosis in zebrafish embryos.Numerous experiments have shown that high-LET heavy ions may cause more DNA breaks than low-LET radiation.When DNA damage is irreparable,cell death occurs.DNA fragmentation is a characteristic feature of apoptosis,which is considered to be one consequence of radiation,via the induction of programmed cell death.展开更多
基金financially supported by the National Key R&D Program of China (2016YFD0400500)
文摘Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and norisoprenoids in pine board might be introduced into Qingke Baijiu.Thus,the terpenoids and norisoprenoids in Qingke Baijiu were investigated by headspace solid phase microextraction(HS-SPME)combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry(GC×GC-TOFMS).The results showed thatγ-terpinene(0.70-530.72μg/L),α-phellandrene(0.34-256.66μg/L),longicyclene(0-38.55μg/L),α-pinene(1.21-35.54μg/L)and limonene(0.93-23.69μg/L)were the top 5 terpenoids/norisoprenoids in Qingke Baijiu.Pulegone was detected and reported for the first time in Baijiu,and the concentrations in Qingke Baijiu were 0.78μg/L(fresh)and 3.90μg/L(7 years old).According to the principal component analysis(PCA)plot,young,aged,and retail Qingke Baijiu could be differentiated clearly.Fold change(FC)and t-tests analysis indicated thatβ-pinene,γ-terpinene,andα-selinene were the most different terpenoids/norisoprenoids between young and aged Qingke Baijiu,and longifolene was the most different terpenoids/norisoprenoids between base and retail Qingke Baijiu.The terpenoids and norisoprenoids,such as(E)-β-ionone,isoborneol andβ-cyclocitral,could be potential markers indicating the ageing process of Qingke Baijiu.
基金supported by National Basic Research Program of China(2010CB834202)the National Natural Science Foundation of China(11305226,11175222)+1 种基金the Scientific Technology Research Projects of Lanzhou City(2015-3-75)the Western Talent Program of Chinese Academy of Sciences(Y460030XB0)
文摘The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.
基金Key Program of National Natural Science Foundation of China (U1432248), National Natural Science Foundation of China (11305226)
文摘The visual system is vital to human health, and the effects of ionizing radiation on vision have received increasing attention. Using a zebrafish model, this study was designed to investigate the apoptosis in eyes induced by carbonion irradiation. Zebrafish embryos at 12 h post-fertilization (hpf) were irradiated using 12C6+ion beams at doses of 2, 4 and 8 Gy.
基金Key Program of National Natural Science Foundation of China (U1432248),National Natural Science Foundation of China (11305226, 11175222).
文摘CO is increasingly appreciated as a signal molecule, due to its protective features, such as anti-inflammatoryfunction[1], anti-apoptosis[2]. Carbon monoxide -releasing molecules (CORMs) represent a group of compounds thatare capable of modulating physiological functions via liberating CO. Among various CORMs synthetized, CORM-3is one of the most promising compounds because it is soluble in water and the half-life to liberate CO in saline was10.6 h, thus imitating the action of CO more closely[3].
基金National Key R&D Program of China(2018YFE0205100)。
文摘Radioresistance serves as the main contributor to treatment failure,bringing about tumour relapse and metas-tasis.Quiescent cancer cells are therapeutically challenging owing to their resistance to most conventional photon treatments that selectively act on proliferating cells^([1,2]).Heavy-ion radiotherapy(RT)has become an increasingly valid treatment option owing to its advantageous dose profile and radio-biological effects compared to conventional RT^([3]).
基金National Key R&D Program of China(2018YFE0205100)。
文摘The radioresistance of cancer cells is a major factor leading to radiotherapy failure and poor prognosis in patients.Quiescent cells are considered a major reason for cancer radioresistance due to their larger hypoxic fraction and stronger repair ability^([1]).Compared with proliferating cancer cells,quiescent cancer cells have lower sensitivity to radiotherapy and chemotherapy,and stronger repair ability^([2,3]).
基金National Key R&D Program of China(2018YFE0205100)。
文摘Cervical carcinoma is one of the most common and lethal gynecological malignancy in women worldwide,and its mortality rates only rank second to breast cancer.Recently,^12C^6+radiotherapy,as a highly intensive local therapy.
基金National Key R&D Program of China(2018YFE0205100)。
文摘Cervical cancer is the second most common malignant tumour threatening women's health.In recent years,heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer,however,radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer[1,2].
基金Key Program of National Natural Science Foundation of China(U1432248)National Key Projects of Research and Development(2016YFC0904600)National Natural Science Foundation of China(11305226,11605255)。
文摘The inhibitory effects of carbon monoxide(CO),generated by Ru(CO)3Cl-glycinate[CO-releasing molecule(CORM-3)],on cell apoptosis in zebrafish embryos induced by ionizing radiation with different linear energy transfer(LET)were studied.Zebrafish embryos at 5 h post-fertilization(hpf)were irradiated with X-ray(low-LET)and carbon-ion(high-LET)with or without pretreatment of CORM-31 h before irradiation.Embryos were divided into four groups:(i)the control group(C),which received no further treatments after rinsed with E3 culture medium;(ii)CORM-3-pretreated group(CORM),which did not receive irradiation after exposed to 10M CORM-3 at 4 hpf;(iii)irradiation(IR)groups,which received X-ray or 12C6+ion irradiation at 5 hpf and(iv)CORM-3-pretreatment+irradiationgroup(CORM+IR),which received 10M CORM-3 treatment 1h before X-ray or 12C6+ion irradiation.The apoptotic signals of living embryos at 24 hpf were detected through staining with the vital dye acridine orange(AO).
基金Key Program of National Natural Science Foundation of China(U1432248),Ministry of Science and Technology National Key R&D project(2016YFC0904600),National Natural Science Foundation of China(11405230,10835011,11205219).
文摘F1Fo ATP synthase,or named ATPase(EC 3.6.1.3)(referred as the same enzyme hereafter)catalyzes the phosphorylation of ADP to ATP by exploiting a the transmembrane proton motive force(Δp)produced by the oxidation of nutrient substrates in mitochondrial respiration in all organisms.However,under peculiar physiological conditions when mitochondrial respiration is impaired which lead to the electrochemical gradient across the inner membrane collapse,the F1Fo ATP synthase switches its catalytic activity from ATP synthesis to ATP hydrolysis for maintaining theΔp.
基金Key Program of National Natural Science Foundation of China(U1432248),National Natural Science Foundation of China(11305226).
文摘All living organisms and ecosystems are permanently exposed to ionizing radiation.Of all the types of ionizing radiation,heavy ions such as 56Fe have the potential to cause the most severe biological effects.We therefore examined the effects and potential mechanisms of iron ion irradiation on the induction of apoptosis in zebrafish embryos.Numerous experiments have shown that high-LET heavy ions may cause more DNA breaks than low-LET radiation.When DNA damage is irreparable,cell death occurs.DNA fragmentation is a characteristic feature of apoptosis,which is considered to be one consequence of radiation,via the induction of programmed cell death.
文摘长双歧杆菌由于其具有的益生功能而备受关注,该文旨在基于全基因组分析,揭示长双歧杆菌W13(Bifidobacterium longum W13)的基因特性,分析其功能基因和代谢通路。结果表明,长双歧杆菌W13隶属于长亚种,通过直系同源蛋白分组比对(evolutionary genealogy of genes:non-supervised orthologous groups,eggNOG)数据库注释,发现氨基酸代谢、碳水化合物代谢是该菌株的主要代谢功能;通过京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)数据库注释,发现菌株中含有较多的群体感应相关基因,表明该菌株可促进肠道稳态。通过碳水化合物活性酶(carbohydrate-active enzymes,CAZy)数据库注释,发现该菌株具有较高的糖合成能力。最后,通过比较基因组分析,发现长双歧杆菌长亚种的遗传多样性和稳定性较高,相较于其他长双歧杆菌长亚种,菌株W13含有其特定的功能基因。综上表明菌株W13具有一定的益生功能,有开发为益生菌的潜力。
