目的:β-葡萄糖苷酶是葡萄酒中结合态香气物质释放的关键酶,但其活性受诸多因素的影响。本研究旨在从酒酒球菌自身耐酸能力的视角去分析评估菌株糖苷酶活性,探索酸胁迫下不同耐酸表型酒酒球菌与其β-葡萄糖苷酶活性之间存在的相关性关...目的:β-葡萄糖苷酶是葡萄酒中结合态香气物质释放的关键酶,但其活性受诸多因素的影响。本研究旨在从酒酒球菌自身耐酸能力的视角去分析评估菌株糖苷酶活性,探索酸胁迫下不同耐酸表型酒酒球菌与其β-葡萄糖苷酶活性之间存在的相关性关系。方法:结合利用离子注入诱变与胁迫环境筛选的方式,获得耐酸(p H 3.0)、酸敏(p H 9.0)突变酒酒球菌菌株,对其β-葡萄糖苷酶的活力进行测定,并筛选3组酸胁迫下表型差异大的β-葡萄糖苷酶基因送样测序。结果:耐酸突变酒酒球菌的β-葡萄糖苷酶酶活力是出发菌株的2~4倍,是相应酸敏突变株的2~7倍。测序结果显示,除菌株a3的β-葡萄糖苷酶基因在108位(G置换成C)和1 232(A置换成T)位处发生了突变外,其余菌株β-葡萄糖苷酶的基因均未发生改变。结论:酒酒球菌β-葡萄糖苷酶活性与菌株酸胁迫能力显著相关(P<0.05)。耐酸胁迫能力越强的菌株,β-葡萄糖苷酶活性越高。展开更多
针对高超声速飞行器飞行过程中颤振边界变动范围大、试验测试难的问题,本文开展了考虑气动热效应的翼面结构地面颤振试验技术研究。首先基于工程法对结构所受的气动加热进行了分析,在此基础上开展了结构的热颤振特性评估并作为地面颤振...针对高超声速飞行器飞行过程中颤振边界变动范围大、试验测试难的问题,本文开展了考虑气动热效应的翼面结构地面颤振试验技术研究。首先基于工程法对结构所受的气动加热进行了分析,在此基础上开展了结构的热颤振特性评估并作为地面颤振试验结果的参考标准。考虑实际飞行中结构温升效应影响,建立了基于多工况点的气动力综合优化降阶算法,确保了整个温升过程的气动力模拟的精度。通过建立基于模糊逻辑比例、积分和微分(Proportional integral derivative,PID)控制的多点协调控制系统,实现了温升过程中时变系统的激振力控制器设计。最终搭建了地面颤振试验系统,按照典型飞行状态对结构的热颤振特性进行了测试,试验测试结果与仿真结果对比相对误差约10%。展开更多
Due to the dynamical character of electromagnetic exciter and the coupling between structure and exciter(s),the actual output force acting on the structure is usually not equal to the exact value that is supposed to b...Due to the dynamical character of electromagnetic exciter and the coupling between structure and exciter(s),the actual output force acting on the structure is usually not equal to the exact value that is supposed to be,especially when multi-exciters are used as actuators to precisely actuate large flexible structure.It is necessary to consider these effects to ensure the force generated by each exciter is the same as required.In this paper,a robust control method is proposed for the multi-input and multi-output(MIMO)structural vibration control system to trace the target actuating force of each exciter.A special signal is designed and put into the coupled mul-ti-exciter-structure system,and the input and output signals of the system are used to build a dynamic model involving both the dynamical characters of the exciters and the structure using the subspace identification method.Considering the uncertainty factors of the multi-exciter/structure system,an H-infinity robust controller is designed to decouple the coupling between structure and exciters based on the identified system model.A MIMO vibration control system combined with a flexible plate and three electromagnetic exciters is adopted to demonstrate the proposed method,both numerical simulation and model experiments showing that the output force of each exciter can trace its target force accurately within the requested frequency band.展开更多
文摘目的:β-葡萄糖苷酶是葡萄酒中结合态香气物质释放的关键酶,但其活性受诸多因素的影响。本研究旨在从酒酒球菌自身耐酸能力的视角去分析评估菌株糖苷酶活性,探索酸胁迫下不同耐酸表型酒酒球菌与其β-葡萄糖苷酶活性之间存在的相关性关系。方法:结合利用离子注入诱变与胁迫环境筛选的方式,获得耐酸(p H 3.0)、酸敏(p H 9.0)突变酒酒球菌菌株,对其β-葡萄糖苷酶的活力进行测定,并筛选3组酸胁迫下表型差异大的β-葡萄糖苷酶基因送样测序。结果:耐酸突变酒酒球菌的β-葡萄糖苷酶酶活力是出发菌株的2~4倍,是相应酸敏突变株的2~7倍。测序结果显示,除菌株a3的β-葡萄糖苷酶基因在108位(G置换成C)和1 232(A置换成T)位处发生了突变外,其余菌株β-葡萄糖苷酶的基因均未发生改变。结论:酒酒球菌β-葡萄糖苷酶活性与菌株酸胁迫能力显著相关(P<0.05)。耐酸胁迫能力越强的菌株,β-葡萄糖苷酶活性越高。
文摘针对高超声速飞行器飞行过程中颤振边界变动范围大、试验测试难的问题,本文开展了考虑气动热效应的翼面结构地面颤振试验技术研究。首先基于工程法对结构所受的气动加热进行了分析,在此基础上开展了结构的热颤振特性评估并作为地面颤振试验结果的参考标准。考虑实际飞行中结构温升效应影响,建立了基于多工况点的气动力综合优化降阶算法,确保了整个温升过程的气动力模拟的精度。通过建立基于模糊逻辑比例、积分和微分(Proportional integral derivative,PID)控制的多点协调控制系统,实现了温升过程中时变系统的激振力控制器设计。最终搭建了地面颤振试验系统,按照典型飞行状态对结构的热颤振特性进行了测试,试验测试结果与仿真结果对比相对误差约10%。
基金supported by the National Natural Science Foundation of China(Grant Nos.11072198,11102162)111 Project of China(Grant No.B07050)
文摘Due to the dynamical character of electromagnetic exciter and the coupling between structure and exciter(s),the actual output force acting on the structure is usually not equal to the exact value that is supposed to be,especially when multi-exciters are used as actuators to precisely actuate large flexible structure.It is necessary to consider these effects to ensure the force generated by each exciter is the same as required.In this paper,a robust control method is proposed for the multi-input and multi-output(MIMO)structural vibration control system to trace the target actuating force of each exciter.A special signal is designed and put into the coupled mul-ti-exciter-structure system,and the input and output signals of the system are used to build a dynamic model involving both the dynamical characters of the exciters and the structure using the subspace identification method.Considering the uncertainty factors of the multi-exciter/structure system,an H-infinity robust controller is designed to decouple the coupling between structure and exciters based on the identified system model.A MIMO vibration control system combined with a flexible plate and three electromagnetic exciters is adopted to demonstrate the proposed method,both numerical simulation and model experiments showing that the output force of each exciter can trace its target force accurately within the requested frequency band.
