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A novel nondestructive detection approach for seed cotton lint percentage using deep learning
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作者 GENG Lijie YAN Pengji +7 位作者 JI Zhikun song Chunyu song shuaifei ZHANG Ruiliang ZHANG Zhifeng ZHAI Yusheng JIANG Liying YANG Kun 《Journal of Cotton Research》 CAS 2024年第2期148-162,共15页
Background The lint percentage of seed cotton is one of the most important parameters for evaluating seed cotton quality and affects its price.The traditional measuring method of lint percentage is labor-intensive and... Background The lint percentage of seed cotton is one of the most important parameters for evaluating seed cotton quality and affects its price.The traditional measuring method of lint percentage is labor-intensive and time-consuming;thus,an efficient and accurate measurement method is needed.In recent years,classification-based deep learning and computer vision have shown promise in solving various classification tasks.Results In this study,we propose a new approach for detecting the lint percentage using MobileNetV2 and transfer learning.The model is deployed on a lint percentage detection instrument,which can rapidly and accurately determine the lint percentage of seed cotton.We evaluated the performance of the proposed approach using a dataset comprising 66924 seed cotton images from different regions of China.The results of the experiments showed that the model with transfer learning achieved an average classification accuracy of 98.43%,with an average precision of 94.97%,an average recall of 95.26%,and an average F1-score of 95.20%.Furthermore,the proposed classification model achieved an average accuracy of 97.22%in calculating the lint percentage,showing no significant difference from the performance of experts(independent-sample t-test,t=0.019,P=0.860).Conclusion This study demonstrated the effectiveness of the MobileNetV2 model and transfer learning in calculating the lint percentage of seed cotton.The proposed approach is a promising alternative to traditional methods,providing a rapid and accurate solution for the industry. 展开更多
关键词 Neural network MobileNetV2 Nondestructive detection Smart agriculture Seed cotton lint percentage
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miRNA-148a-3p对山羊卵巢颗粒细胞功能的影响
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作者 王钰锟 李碧筠 +8 位作者 张义语 丁文飞 王磊 唐雪 宋帅飞 姚慧 黄德利 徐德军 赵中权 《中国兽医学报》 CAS CSCD 北大核心 2023年第5期1035-1043,共9页
为探究miRNA-148a-3p在山羊卵巢颗粒细胞中的作用,通过过表达或抑制miRNA-148a-3p、qRT-PCR、Western blot、流式细胞术、ELISA等方法,探究miR-148a-3p对卵巢颗粒细胞增殖、凋亡、自噬及类固醇激素分泌的影响。结果发现,过表达miR-148a... 为探究miRNA-148a-3p在山羊卵巢颗粒细胞中的作用,通过过表达或抑制miRNA-148a-3p、qRT-PCR、Western blot、流式细胞术、ELISA等方法,探究miR-148a-3p对卵巢颗粒细胞增殖、凋亡、自噬及类固醇激素分泌的影响。结果发现,过表达miR-148a-3p显著促进卵巢颗粒细胞增殖和PCNA表达,抑制miR-148a-3p显著抑制卵巢颗粒细胞增殖和PCNA表达。过表达miR-148a-3p抑制卵巢颗粒细胞凋亡,极显著下调细胞凋亡率,显著上调Bcl-2/Bax的mRNA和蛋白比值,抑制miR-148a-3p促进卵巢颗粒细胞凋亡。过表达miR-148a-3p显著促进卵巢颗粒细胞自噬,上调LC3-Ⅱ/LC3-Ⅰ比值,抑制miR-148a-3p上调p62表达。过表达miR-148a-3p显著促进卵巢颗粒细胞孕酮分泌,并上调3β-HSD、StAR、CYP11A1的mRNA和蛋白表达。虽然抑制miR-148a-3p下调孕酮水平,但对3β-HSD、StAR、CYP11A1及CYP19A1的mRNA和蛋白表达无显著性影响。结果表明,miR-148a-3p可促进卵巢颗粒细胞增殖、抑制细胞凋亡、诱导自噬并刺激孕酮的合成。 展开更多
关键词 miR-148a-3p 山羊卵巢颗粒细胞 增殖 凋亡 自噬 孕酮
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