An innovative in-situ stabilisation treatment followed by ex-situ sediment composting was tested for its ability to treat and dispose of heavy-metal-polluted sediments in a river near the Chinese Pearl Delta. First, p...An innovative in-situ stabilisation treatment followed by ex-situ sediment composting was tested for its ability to treat and dispose of heavy-metal-polluted sediments in a river near the Chinese Pearl Delta. First, polluted sediments were treated in-situ to stabilise the heavy metals. Then the treated sediments were dredged, dewatered and sent for high temperature aerobic composting （HTAC） treatment. Finally, the compost products were used as a fertiliser for fiver bank plants. The stabilisation efficiency of heavy metals during the process was investigated and the results are as follows： （1） using in-situ stabilisation, the extraction concentrations of Cu, Zn and Pb were reduced by 65.0%, 82.2% and 90.0%, respectively, which are much lower than the national standard given in the Identification Standard for Hazardous Waste （GB5085.3-1996）; （2） chemical fraction analysis showed that heavy metals were further stabilized during the HTAC treatment; （3） the concentrations of Cu, Zn and Pb in rainwater leachate through the river bank met the level of class V in the Environmental Quality Standards for Surface Water in China （GB3838-2002）. Therefore, using this new process, the toxicities of heavy metals in sediments were reduced markedly.展开更多
An evaluation system of hydrocarbon-bearing availability of fault traps was established based on the comprehensive analysis of fault segment growth history,fine reservoir anatomy and geochemistry tracing,with the Qiko...An evaluation system of hydrocarbon-bearing availability of fault traps was established based on the comprehensive analysis of fault segment growth history,fine reservoir anatomy and geochemistry tracing,with the Qikou sag in the Bohai Bay Basin as target area.The displacement/separation transform and displacement gradient method were used to prove the interpretation reliability of fault traps.The method of maximum throw subtraction was used to recover the history of fault growth and determine the availability of the forming period of fault traps.Based on the quantitative relationship between shale gouge ratio and cross-fault pressure difference of known reservoirs in southern Qikou sag,the critical shale gouge ratio of fault lateral sealing was calculated at 20%,and the quantitative evaluation chart based on the relationship of"fault throw–sand-formation ratio and hydrocarbon column height"was constructed.Based on the results of reservoir fine anatomy and quantitative fluorescence tracing test shale smear factor method is suitable for evaluating the vertical sealing of faults in the caprock of the middle submember of first member of Paleogene Shahejie Formation,and the shale smear factor critical value is 3.5.The juxtaposition thickness method is suitable for evaluating vertical sealing of faults in the caprock of the second member of Paleogene Dongying Formation,and the critical juxtaposition thickness of fault is 70–80 m.By combining four factors,the availability of fault trap interpretation,the availability of the forming period of fault trap,the availability of fault lateral sealing and the availability of fault vertical sealing,the comprehensive evaluation chart on hydrocarbon-bearing availability of fault traps in Qikou sag has been established,which provides a reasonable basis for risk assessment of fault traps.展开更多
Talin is an integrin-binding protein located at focal adhesion site and serves as both an adapter and a force transmitter. Its integrin binding activity is regulated by the intramolecular autoinhibition interaction be...Talin is an integrin-binding protein located at focal adhesion site and serves as both an adapter and a force transmitter. Its integrin binding activity is regulated by the intramolecular autoinhibition interaction between its F3 and RS domains. Here, we used atomic force microscopy to measure the strength of talin autoinhibition complex. Our results suggest that the lifetime of talin autoinhibition complex shows weak catch bond behavior and does not change significantly at smaller forces, while it drops rapidly at larger forces(>10 p N). Moreover, besides the complex conformation revealed by crystal structure, our molecular dynamics(MD) simulations indicate the possible existence of another stable conformation. Further analysis indicates that forces may regulate the equilibrium of the two stable binding states and result in the non-exponential force dependence of the binding lifetime. Our findings reveal a negative regulation mechanism on talin activation and provide a new point of view on the function of talin in focal adhesion.展开更多
基金supported by the Scientific and Tech- nological Planning Project of Guangdong Province (No. 2003A3040404)the Guangdong & Hong Kong Tech- nology Cooperation Funding (No. 2006A36702001)
文摘An innovative in-situ stabilisation treatment followed by ex-situ sediment composting was tested for its ability to treat and dispose of heavy-metal-polluted sediments in a river near the Chinese Pearl Delta. First, polluted sediments were treated in-situ to stabilise the heavy metals. Then the treated sediments were dredged, dewatered and sent for high temperature aerobic composting （HTAC） treatment. Finally, the compost products were used as a fertiliser for fiver bank plants. The stabilisation efficiency of heavy metals during the process was investigated and the results are as follows： （1） using in-situ stabilisation, the extraction concentrations of Cu, Zn and Pb were reduced by 65.0%, 82.2% and 90.0%, respectively, which are much lower than the national standard given in the Identification Standard for Hazardous Waste （GB5085.3-1996）; （2） chemical fraction analysis showed that heavy metals were further stabilized during the HTAC treatment; （3） the concentrations of Cu, Zn and Pb in rainwater leachate through the river bank met the level of class V in the Environmental Quality Standards for Surface Water in China （GB3838-2002）. Therefore, using this new process, the toxicities of heavy metals in sediments were reduced markedly.
基金Supported by the National Natural Science Foundation of China(41972157)Natural Science Foundation of Heilongjiang Province,China(TD2019D001)+1 种基金Natural Science Foundation of Heilongjiang Province,China(QC2018041)PetroChina Major Project of Ministry of Science and Technology(2019D-0706)。
文摘An evaluation system of hydrocarbon-bearing availability of fault traps was established based on the comprehensive analysis of fault segment growth history,fine reservoir anatomy and geochemistry tracing,with the Qikou sag in the Bohai Bay Basin as target area.The displacement/separation transform and displacement gradient method were used to prove the interpretation reliability of fault traps.The method of maximum throw subtraction was used to recover the history of fault growth and determine the availability of the forming period of fault traps.Based on the quantitative relationship between shale gouge ratio and cross-fault pressure difference of known reservoirs in southern Qikou sag,the critical shale gouge ratio of fault lateral sealing was calculated at 20%,and the quantitative evaluation chart based on the relationship of"fault throw–sand-formation ratio and hydrocarbon column height"was constructed.Based on the results of reservoir fine anatomy and quantitative fluorescence tracing test shale smear factor method is suitable for evaluating the vertical sealing of faults in the caprock of the middle submember of first member of Paleogene Shahejie Formation,and the shale smear factor critical value is 3.5.The juxtaposition thickness method is suitable for evaluating vertical sealing of faults in the caprock of the second member of Paleogene Dongying Formation,and the critical juxtaposition thickness of fault is 70–80 m.By combining four factors,the availability of fault trap interpretation,the availability of the forming period of fault trap,the availability of fault lateral sealing and the availability of fault vertical sealing,the comprehensive evaluation chart on hydrocarbon-bearing availability of fault traps in Qikou sag has been established,which provides a reasonable basis for risk assessment of fault traps.
基金supported by the National Basic Research Program of China(2014CB910202)National Natural Science Foundation of China(11302240,31070827 and 31222022)
文摘Talin is an integrin-binding protein located at focal adhesion site and serves as both an adapter and a force transmitter. Its integrin binding activity is regulated by the intramolecular autoinhibition interaction between its F3 and RS domains. Here, we used atomic force microscopy to measure the strength of talin autoinhibition complex. Our results suggest that the lifetime of talin autoinhibition complex shows weak catch bond behavior and does not change significantly at smaller forces, while it drops rapidly at larger forces(>10 p N). Moreover, besides the complex conformation revealed by crystal structure, our molecular dynamics(MD) simulations indicate the possible existence of another stable conformation. Further analysis indicates that forces may regulate the equilibrium of the two stable binding states and result in the non-exponential force dependence of the binding lifetime. Our findings reveal a negative regulation mechanism on talin activation and provide a new point of view on the function of talin in focal adhesion.
