The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts＇ behaviors including cell morphology, adhesion, proliferation, differentiation, and m...The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts＇ behaviors including cell morphology, adhesion, proliferation, differentiation, and mineralization in vitro. A new micro/nano-hybrid topography surface was fabricated on commercial pure titanium（Cp Ti） by a two-step sandblasted acid-etching and subsequent alkali-and heattreatment（SA-AH）. The conventional sandblasted/acid-etching（SA） treatment and alkali and heat（AH） treatment were also carried out on the Cp Ti as controls. Surface microstructures of the Ti disc samples were assessed by scanning electron microscopy（SEM）. The neonatal rat calvaria-derived osteoblasts were seeded on these discs and the initial cell morphology was evaluated by SEM and immunofluorescence. Initial adhesion of the cells was then assayed by DAPI staining at 1, 2, and 4 h after seeding. The Cell Counting Kit-8（CCact K8） assay, gene expression of osteoblastic markers（ALP, Col 1, OCN, BSP, OSX, Cbfα1） and Alizarin Red S staining assays were monitored respectively for cell proliferations, differentiation and mineralization. The results show significant differences in osteoblast＇s behaviors on the four kinds of Ti surfaces. Compared with Cp Ti surface, the SA and AH treatment can significantly promote cell adhesion, differentiation and mineralization of osteoblasts. In particular, the combined SA and AH treatments exhibit synergistic effects in comparison with the treatment of SA and AH individually, and are more favorable for stimulating a series of osteogenous responses from cell adhesion to mineralization of osteoblasts. In summary, this study provides some new evidence that the integrated micro/nanostructure on the Cp Ti surface may promote bone osseointegration between the Ti implantbone interfaces in vitro.展开更多
目的比较采用带孔金属骨针与普通克氏针加钛缆张力带治疗MayoⅡA型尺骨鹰嘴骨折的临床疗效。方法回顾性分析自2012年6月至2018年6月我们收治的35例MayoⅡA型尺骨鹰嘴骨折患者,术后随访30例,失访5例。其中14例采用带孔金属骨针加钛缆张...目的比较采用带孔金属骨针与普通克氏针加钛缆张力带治疗MayoⅡA型尺骨鹰嘴骨折的临床疗效。方法回顾性分析自2012年6月至2018年6月我们收治的35例MayoⅡA型尺骨鹰嘴骨折患者,术后随访30例,失访5例。其中14例采用带孔金属骨针加钛缆张力带治疗(A组),16例采用普通克氏针加钛缆张力带治疗(B组)。比较两组切口长度、手术时间、骨折愈合时间、肘关节活动度及并发症。采用上肢功能评分(disabilities of the arm,shoulder and hand,DASH)和肘关节Mayo功能评分(Mayo elbow performance score,MEPS)评估两组肘关节功能。结果术后30例获得随访,时间为6~24个月,平均13个月。两组切口长度,肘关节屈曲、背伸、旋前、旋后活动度,DASH和MEPS评分差异无统计学意义(P>0.05)。A组手术时间、骨折愈合时间短于B组(P<0.05)。A组无皮肤激惹、切口感染及内固定松动等并发症;B组2例发生皮肤激惹,1例切口感染,1例内固定失败。结论采用带孔金属骨针钛缆张力带治疗MayoⅡA型尺骨鹰嘴骨折,能缩短手术时间和骨折愈合时间,明显减少术后并发症的发生,且临床操作简单,易于推广。展开更多
基金Funded by the Natural Science Fundation of Zhejiang Province(Nos.Y2080956 and Y4110169)the National Natural Science Foundation of China(Nos.51102211,and 20934003)the Science and Technique Plans of Wenzhou City(Nos.Y20070093 and H20100076)
文摘The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts＇ behaviors including cell morphology, adhesion, proliferation, differentiation, and mineralization in vitro. A new micro/nano-hybrid topography surface was fabricated on commercial pure titanium（Cp Ti） by a two-step sandblasted acid-etching and subsequent alkali-and heattreatment（SA-AH）. The conventional sandblasted/acid-etching（SA） treatment and alkali and heat（AH） treatment were also carried out on the Cp Ti as controls. Surface microstructures of the Ti disc samples were assessed by scanning electron microscopy（SEM）. The neonatal rat calvaria-derived osteoblasts were seeded on these discs and the initial cell morphology was evaluated by SEM and immunofluorescence. Initial adhesion of the cells was then assayed by DAPI staining at 1, 2, and 4 h after seeding. The Cell Counting Kit-8（CCact K8） assay, gene expression of osteoblastic markers（ALP, Col 1, OCN, BSP, OSX, Cbfα1） and Alizarin Red S staining assays were monitored respectively for cell proliferations, differentiation and mineralization. The results show significant differences in osteoblast＇s behaviors on the four kinds of Ti surfaces. Compared with Cp Ti surface, the SA and AH treatment can significantly promote cell adhesion, differentiation and mineralization of osteoblasts. In particular, the combined SA and AH treatments exhibit synergistic effects in comparison with the treatment of SA and AH individually, and are more favorable for stimulating a series of osteogenous responses from cell adhesion to mineralization of osteoblasts. In summary, this study provides some new evidence that the integrated micro/nanostructure on the Cp Ti surface may promote bone osseointegration between the Ti implantbone interfaces in vitro.
文摘目的比较采用带孔金属骨针与普通克氏针加钛缆张力带治疗MayoⅡA型尺骨鹰嘴骨折的临床疗效。方法回顾性分析自2012年6月至2018年6月我们收治的35例MayoⅡA型尺骨鹰嘴骨折患者,术后随访30例,失访5例。其中14例采用带孔金属骨针加钛缆张力带治疗(A组),16例采用普通克氏针加钛缆张力带治疗(B组)。比较两组切口长度、手术时间、骨折愈合时间、肘关节活动度及并发症。采用上肢功能评分(disabilities of the arm,shoulder and hand,DASH)和肘关节Mayo功能评分(Mayo elbow performance score,MEPS)评估两组肘关节功能。结果术后30例获得随访,时间为6~24个月,平均13个月。两组切口长度,肘关节屈曲、背伸、旋前、旋后活动度,DASH和MEPS评分差异无统计学意义(P>0.05)。A组手术时间、骨折愈合时间短于B组(P<0.05)。A组无皮肤激惹、切口感染及内固定松动等并发症;B组2例发生皮肤激惹,1例切口感染,1例内固定失败。结论采用带孔金属骨针钛缆张力带治疗MayoⅡA型尺骨鹰嘴骨折,能缩短手术时间和骨折愈合时间,明显减少术后并发症的发生,且临床操作简单,易于推广。
