Objective:To observe the effects of and explore the underlying mechanisms of Periplogenin(PPG)on the regulation of proliferation,apoptosis and migration of hepatocellular carcinoma Hep3B cell.Methods:Hep3B cells were ...Objective:To observe the effects of and explore the underlying mechanisms of Periplogenin(PPG)on the regulation of proliferation,apoptosis and migration of hepatocellular carcinoma Hep3B cell.Methods:Hep3B cells were divided into control group and PPG group.The Hep3B cells in the control group were not treated with PPG,and the other in the PPG group were intervened with 2.5,5 and 12.5µmol/mL PPG for 48 h.Cell viability was measured using the CCK-8 assay.Cell morphological changes was observed using by light microscope;the apoptotic state was observed by DAPI/PI staining.The colony-formation assay and scratch method was used for detecting the cell clone forming ability and cell migration ability,respectively.The cell apoptosis-related proteins Caspase-3,Cleaved Caspase-3,Bcl-2 and cell migration-related protein Matrix metalloproteinase-9(MMP-9)were detected byWestern Blot.Results:Compared with the control group,the cell proliferation level and the cell number in each PPG group were decreased,accompanying by cell volume reduction,chromatin pyknosis,and nuclear rupture in Hep3B cells.After PPG treatment,the clone formation level and cell mobility of Hep3B cells were decreased.Compared with the control group,the level of Bcl-2 protein was decreased,while the proportion of Cleaved Caspase-3/Caspase-3 protein was increased.The expression of MMP-9 proteins was decreased,with significantly statistically differences(P<0.05).Conclusion:Periplogenin could inhibit the proliferation,and migration of Hep3B cells and also promote the cell apoptosis in a dose-dependent manner,which might be related to the activation of Caspase signaling pathway and inhibition of MMP-9 activity.展开更多
基金National Natural Science Foundation of China(82205209)Shenzhen Science and Technology Program(JCYJ20210324120405015)。
文摘Objective:To observe the effects of and explore the underlying mechanisms of Periplogenin(PPG)on the regulation of proliferation,apoptosis and migration of hepatocellular carcinoma Hep3B cell.Methods:Hep3B cells were divided into control group and PPG group.The Hep3B cells in the control group were not treated with PPG,and the other in the PPG group were intervened with 2.5,5 and 12.5µmol/mL PPG for 48 h.Cell viability was measured using the CCK-8 assay.Cell morphological changes was observed using by light microscope;the apoptotic state was observed by DAPI/PI staining.The colony-formation assay and scratch method was used for detecting the cell clone forming ability and cell migration ability,respectively.The cell apoptosis-related proteins Caspase-3,Cleaved Caspase-3,Bcl-2 and cell migration-related protein Matrix metalloproteinase-9(MMP-9)were detected byWestern Blot.Results:Compared with the control group,the cell proliferation level and the cell number in each PPG group were decreased,accompanying by cell volume reduction,chromatin pyknosis,and nuclear rupture in Hep3B cells.After PPG treatment,the clone formation level and cell mobility of Hep3B cells were decreased.Compared with the control group,the level of Bcl-2 protein was decreased,while the proportion of Cleaved Caspase-3/Caspase-3 protein was increased.The expression of MMP-9 proteins was decreased,with significantly statistically differences(P<0.05).Conclusion:Periplogenin could inhibit the proliferation,and migration of Hep3B cells and also promote the cell apoptosis in a dose-dependent manner,which might be related to the activation of Caspase signaling pathway and inhibition of MMP-9 activity.
