目的回顾性分析机械取栓对急性脑梗死患者临床疗效及预后的影响因素。方法回顾性分析2019年10月—2021年12月齐齐哈尔医学院附属第三医院通过机械取栓治疗的31例急性大动脉闭塞性脑梗患者的临床资料。根据术后3个月预后情况将入组患者...目的回顾性分析机械取栓对急性脑梗死患者临床疗效及预后的影响因素。方法回顾性分析2019年10月—2021年12月齐齐哈尔医学院附属第三医院通过机械取栓治疗的31例急性大动脉闭塞性脑梗患者的临床资料。根据术后3个月预后情况将入组患者分为预后良好组(mRS≤2分,n=16)和预后不良组(mRS≥3分,n=15)。通过单变量和多变量逻辑回归分析机械取栓除术后急性脑梗死患者的潜在预后因素。结果与预后良好组比较,预后不良组术前、术后24 h NIHSS评分更高,房颤史比例更高,ASPECT评分更低,差异有统计学意义(t=2.221、3.668、4.850,P<0.05)。多因素Logistic回归分析显示,术后24 h NIHSS评分、ASPECT评分均是影响急性脑梗死患者机械取栓预后的独立影响因素(OR=1.479、0.293,P<0.05)。结论术后24 h NIHSS评分高、ASPECT评分低是急性脑梗死患者机械取栓治疗预后不佳的危险因素。展开更多
OBJECTIVE:To investigate the synergistic effects of polyphyllin Ⅰ(PPⅠ)combined with tumor necrosis factorrelated apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-...OBJECTIVE:To investigate the synergistic effects of polyphyllin Ⅰ(PPⅠ)combined with tumor necrosis factorrelated apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling pathway.METHODS:Cell viability,apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry assays.The morphology of cancer cells was observed with inverted phase contrast microscope.The migration and invasion abilities were examined by xCELLigence real time cell analysis DP system and transwell assays.The expressions of poly(adenosine diphosphate-ribose)polymerase,C-Myc,Cyclin B1,cyclin-dependent kinases 1,N-cadherin,Vimentin,Active-β-catenin,β-catenin,p-glycogen synthase kinase 3β(GSK-3β)and GSK-3βwere determined by Western blotting assay.RESULTS:PPⅠ sensitized TRAIL-induced decrease of viability,migration and invasion,as well as increase of apoptosis and cell cycle arrest of MG-63 and U-2 OS osteosarcoma cells.The synergistic effect of PPⅠwith TRAIL in inhibiting the growth of osteosarcoma cells was at least partially realized through the inactivation of Wnt/β-catenin signaling pathway.CONCLUSION:The combination of PPⅠ and TRAIL is potentially a novel treatment strategy of osteosarcoma.展开更多
文摘目的回顾性分析机械取栓对急性脑梗死患者临床疗效及预后的影响因素。方法回顾性分析2019年10月—2021年12月齐齐哈尔医学院附属第三医院通过机械取栓治疗的31例急性大动脉闭塞性脑梗患者的临床资料。根据术后3个月预后情况将入组患者分为预后良好组(mRS≤2分,n=16)和预后不良组(mRS≥3分,n=15)。通过单变量和多变量逻辑回归分析机械取栓除术后急性脑梗死患者的潜在预后因素。结果与预后良好组比较,预后不良组术前、术后24 h NIHSS评分更高,房颤史比例更高,ASPECT评分更低,差异有统计学意义(t=2.221、3.668、4.850,P<0.05)。多因素Logistic回归分析显示,术后24 h NIHSS评分、ASPECT评分均是影响急性脑梗死患者机械取栓预后的独立影响因素(OR=1.479、0.293,P<0.05)。结论术后24 h NIHSS评分高、ASPECT评分低是急性脑梗死患者机械取栓治疗预后不佳的危险因素。
基金National Key R&D Program of China:Cooperating Studies on Measurement Technologies of Human Phenome and Crossscale Correlation of Phenotypic Data(No.2020YFE0201600)National Nature Science Foundation:Study on LncRNA-CCDC18-AS1 Mediated Osteosarcoma Occurrence by Activating YAP/TAZ and Tumor Microenvironment M2 TAM-dependent Lung Metastasis,and Efficacy/mechanism of Removing Blood Stasis/clearing heat/eliminating Toxic Material Principle(No.81973877)+2 种基金Mechanism Study on m6A Methyltransferase RBM15 Mediated YAP Epigenetic Modification to Promote Osteosarcoma Lung Metastasis through Lymphatic System and Management with Qichong Powder(No.82174408)Shanghai Collaborative Innovation Center of Industrial Transformation of Hospital TCM Preparation:Preclinical Study on the Treatment of Osteosarcoma with Qingre Jiedu GranulesResearch Projects within Budget of Shanghai University of Traditional Chinese Medicine:the Research on the Mechanism of the HIPK3 Activation of Wnt/β-catenin Induction the Osteosarcoma and the Intervention of Banmao Decoction(No.2021LK047)。
文摘OBJECTIVE:To investigate the synergistic effects of polyphyllin Ⅰ(PPⅠ)combined with tumor necrosis factorrelated apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling pathway.METHODS:Cell viability,apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry assays.The morphology of cancer cells was observed with inverted phase contrast microscope.The migration and invasion abilities were examined by xCELLigence real time cell analysis DP system and transwell assays.The expressions of poly(adenosine diphosphate-ribose)polymerase,C-Myc,Cyclin B1,cyclin-dependent kinases 1,N-cadherin,Vimentin,Active-β-catenin,β-catenin,p-glycogen synthase kinase 3β(GSK-3β)and GSK-3βwere determined by Western blotting assay.RESULTS:PPⅠ sensitized TRAIL-induced decrease of viability,migration and invasion,as well as increase of apoptosis and cell cycle arrest of MG-63 and U-2 OS osteosarcoma cells.The synergistic effect of PPⅠwith TRAIL in inhibiting the growth of osteosarcoma cells was at least partially realized through the inactivation of Wnt/β-catenin signaling pathway.CONCLUSION:The combination of PPⅠ and TRAIL is potentially a novel treatment strategy of osteosarcoma.
