AIM： To investigate the role of survivin expression in the pathogenesis of colorectal carcinoma. METHODS： Immunohistochemistry S-P method and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （T...AIM： To investigate the role of survivin expression in the pathogenesis of colorectal carcinoma. METHODS： Immunohistochemistry S-P method and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNE） were used to detect the expression of survivin and apoptotic cell in situ in colorectal cancerous tissues, para-cancerous tissues and normal tissues of 48 cases of colorectal carcinoma. RESULTS： The survivin positive unit （PU） was higher in cancerous tissues （38.76±5.14） than in para-cancerous （25.17±7.26） or normal tissues （0.57±0.03） （P〈0.05）. The apoptosis index （AI） of para-cancerous tissues was （7.51±2.63%） higher than cancerous tissues （4.65±1.76%）. The expression of survivin was associated with pathological grade, lymph node metastasis and Dukes stage of colorectal carcinoma. CONCLUSION： Survivin expression may play an important role in carcinogenesis of colorectal carcinoma and may be associated with malignant biological behaviors of colorectal carcinoma.展开更多
AIM: To observe the effect of response-guided add-on therapy with adefovir(ADV) and lamivudine(LAM) in cirrhotic hepatitis B(CHB) patients.METHODS: A total of 100 patients with CHB and cirrhosis were divided into thre...AIM: To observe the effect of response-guided add-on therapy with adefovir(ADV) and lamivudine(LAM) in cirrhotic hepatitis B(CHB) patients.METHODS: A total of 100 patients with CHB and cirrhosis were divided into three arms according to hepatitis B virus(HBV) DNA level after 24 wk LAM monotherapy: Arm A(complete response, HBV DNA ≤ 60 IU/m L, n = 49), Arm B(partial response, HBV DNA: 60-2000 IU/m L, n = 31) and Arm C(inadequate response, HBV DNA > 2000 IU/m L, n = 20). ADV was added to LAM at week 48 in Arms A and B, but at week 24 in Arm C. Virological response, YMDD mutations, biochemical response, and liver function were evaluated.RESULTS: Comparison of the three arms demonstrated that early complete virologic response at week 24was associated with maintained viral suppression(undetectable rate of HBV DNA at week 144 was 95.96%, 66.67% and 35.29%, respectively, P = 0.000) and reduced YMDD mutations(mutation rate at week 144 was 0%, 3.23% and 15%, respectively, P = 0.015) after 144 wk treatment. For patients who failed to achieve complete virological response at week 24, switching to combination therapy further decreased HBV DNA level by 1 log10 IU/m L. All three arms obtained biochemical benefits including decline of alanine aminotransferase and elevation of albumin. In patients who developed HBV DNA breakthrough for YMDD mutations, ADV add-on therapy did not induce further multiple drug resistance to LAM or ADV.CONCLUSION: Optimized response-guided add-on therapy of ADV and LAM maintains long-term suppression of HBV DNA and improves liver function in CHB patients with compensated liver cirrhosis.展开更多
Objective:To explore the expression and prognostic significance of ADHs in Hepatocellular carcinoma(HCC).Methods:The clinical data in this study were obtained from The Cancer Genome Atlas(TCGA).The expression of ADHs ...Objective:To explore the expression and prognostic significance of ADHs in Hepatocellular carcinoma(HCC).Methods:The clinical data in this study were obtained from The Cancer Genome Atlas(TCGA).The expression of ADHs was differentially analyzed in normal liver tissues and HCC using the Metabolic gEne RApid Visualizer and the TCGA database,and the differentially expressed ADHs were selected for kaplan-meier survival analysis.Cox analysis was performed to select factors that may influence the prognosis of HCC and to verify independent risk factors for HCC patients.The interaction among ADHs was explored at the gene level and protein expression level through GeneMAMIA and STRING,and the functional enrichment analysis of ADH family was carried out using DAVID bioinformatics resources.Results:The expression of ADH1A,ADH1B,ADH1C,ADH4 and ADH7 in HCC is low.The low-expression groups of ADH1A,ADH1B,ADH1C and ADH4 had poor survival rates,which may be related to the poor prognosis of HCC patients.Univariate Cox regression analysis showed that the expression levels of ADH1A,ADH1C and ADH4,as well as the clinical stage,T stage and M stage of the tumor were closely related to the overall survival rate of the patients.Multivariate Cox regression analysis further suggested that the low expression of ADH1A,ADH1C and ADH4 was an independent risk factor affecting the prognosis of HCC patients.There is a pathway between ADH1A-ADH1B,ADH1B-ADH1C,ADH1A-ADH1C,and the ADHs is closely related to Esterase D and Aldehyde dehydrogenase.The ADHs is mainly involved in biological processes such as Ethanol oxidation and Retinol metabolism,and plays a biological role in Glycolysis/Gluconeogenesis,Chemical carcinogenesis and Metabolism of xenobiotics by cytochrome P450.Conclusion:ADH1A,ADH1C and ADH4 may be biomarkers for the prognosis of HCC,providing reference value for the practical application of ADHs in HCC.展开更多
Objective:To analyze the differential expression and function prediction of miRNAs in Hepatocellular carcinoma (HCC) based on Bioinformatics, and to provide new ideas for early diagnosis and late intervention of HCC.M...Objective:To analyze the differential expression and function prediction of miRNAs in Hepatocellular carcinoma (HCC) based on Bioinformatics, and to provide new ideas for early diagnosis and late intervention of HCC.Methods: The expression dataset of HCC non-coding RNA was obtained by GEO database, and differential miRNAs were screened and visualized by limma R package and pheatmap R package using R (v 3.6.0). Transcriptional factors of differential miRNAs were predicted, signaling pathway analysis and GO enrichment analysis were performed and visualized.Results: A total of 37 miRNAs were selected in HCC tissues with differential expressions, and the significant transcription factors were EGR1, POU2F1, SP1, NKX6-1, MEF2A, FOXA1, SP4, NFIC, RREB1, ARID3A, HOXA5, HNF4A, HOXD8, RORA, LHX3, POU3F2, FOXJ2, POU5F1, NKX2-1, SOX1. Differential miRNAs were found to be mainly located in the Nucleus and Cytoplasm, It has molecular functions such as Protein serine/threonine kinase activity, Receptor signaling complex scaffold activity, Transcription regulator activity and Transcription factor activity, Involved in Nucleic acid metabolism and Signal transduction and Cell communication. Through The epidermal growth factor receptor (EGFR), IGF-1,γ-IFN, Plasma membrane estrogen receptor signaling pathways play a role, and differential miRNAs is associated with VEGF, VEGFR, PDGF, Hepatocyte Growth Factor Receptor (c-Met), Protein serine/threonine kinase mediated signaling events.Conclusion:Differential mirnas in hepatocellular carcinoma are closely related to tumor development and progression, and differential miRNAs can be used as potential markers for early diagnosis of HCC, providing new ideas for late intervention.展开更多
文摘AIM： To investigate the role of survivin expression in the pathogenesis of colorectal carcinoma. METHODS： Immunohistochemistry S-P method and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNE） were used to detect the expression of survivin and apoptotic cell in situ in colorectal cancerous tissues, para-cancerous tissues and normal tissues of 48 cases of colorectal carcinoma. RESULTS： The survivin positive unit （PU） was higher in cancerous tissues （38.76±5.14） than in para-cancerous （25.17±7.26） or normal tissues （0.57±0.03） （P〈0.05）. The apoptosis index （AI） of para-cancerous tissues was （7.51±2.63%） higher than cancerous tissues （4.65±1.76%）. The expression of survivin was associated with pathological grade, lymph node metastasis and Dukes stage of colorectal carcinoma. CONCLUSION： Survivin expression may play an important role in carcinogenesis of colorectal carcinoma and may be associated with malignant biological behaviors of colorectal carcinoma.
基金Supported by Grants from Key Medical Specialties Fund of Shanghai Municipal Health Bureau(partially),No.05II 011 2-1Glaxo Smith Kline(China)Investment Co,Ltd,Project 110353
文摘AIM: To observe the effect of response-guided add-on therapy with adefovir(ADV) and lamivudine(LAM) in cirrhotic hepatitis B(CHB) patients.METHODS: A total of 100 patients with CHB and cirrhosis were divided into three arms according to hepatitis B virus(HBV) DNA level after 24 wk LAM monotherapy: Arm A(complete response, HBV DNA ≤ 60 IU/m L, n = 49), Arm B(partial response, HBV DNA: 60-2000 IU/m L, n = 31) and Arm C(inadequate response, HBV DNA > 2000 IU/m L, n = 20). ADV was added to LAM at week 48 in Arms A and B, but at week 24 in Arm C. Virological response, YMDD mutations, biochemical response, and liver function were evaluated.RESULTS: Comparison of the three arms demonstrated that early complete virologic response at week 24was associated with maintained viral suppression(undetectable rate of HBV DNA at week 144 was 95.96%, 66.67% and 35.29%, respectively, P = 0.000) and reduced YMDD mutations(mutation rate at week 144 was 0%, 3.23% and 15%, respectively, P = 0.015) after 144 wk treatment. For patients who failed to achieve complete virological response at week 24, switching to combination therapy further decreased HBV DNA level by 1 log10 IU/m L. All three arms obtained biochemical benefits including decline of alanine aminotransferase and elevation of albumin. In patients who developed HBV DNA breakthrough for YMDD mutations, ADV add-on therapy did not induce further multiple drug resistance to LAM or ADV.CONCLUSION: Optimized response-guided add-on therapy of ADV and LAM maintains long-term suppression of HBV DNA and improves liver function in CHB patients with compensated liver cirrhosis.
基金supported by Wuhan Municipal Project of Scientific Problem Tackling(No.201161038342-02)。
文摘Objective:To explore the expression and prognostic significance of ADHs in Hepatocellular carcinoma(HCC).Methods:The clinical data in this study were obtained from The Cancer Genome Atlas(TCGA).The expression of ADHs was differentially analyzed in normal liver tissues and HCC using the Metabolic gEne RApid Visualizer and the TCGA database,and the differentially expressed ADHs were selected for kaplan-meier survival analysis.Cox analysis was performed to select factors that may influence the prognosis of HCC and to verify independent risk factors for HCC patients.The interaction among ADHs was explored at the gene level and protein expression level through GeneMAMIA and STRING,and the functional enrichment analysis of ADH family was carried out using DAVID bioinformatics resources.Results:The expression of ADH1A,ADH1B,ADH1C,ADH4 and ADH7 in HCC is low.The low-expression groups of ADH1A,ADH1B,ADH1C and ADH4 had poor survival rates,which may be related to the poor prognosis of HCC patients.Univariate Cox regression analysis showed that the expression levels of ADH1A,ADH1C and ADH4,as well as the clinical stage,T stage and M stage of the tumor were closely related to the overall survival rate of the patients.Multivariate Cox regression analysis further suggested that the low expression of ADH1A,ADH1C and ADH4 was an independent risk factor affecting the prognosis of HCC patients.There is a pathway between ADH1A-ADH1B,ADH1B-ADH1C,ADH1A-ADH1C,and the ADHs is closely related to Esterase D and Aldehyde dehydrogenase.The ADHs is mainly involved in biological processes such as Ethanol oxidation and Retinol metabolism,and plays a biological role in Glycolysis/Gluconeogenesis,Chemical carcinogenesis and Metabolism of xenobiotics by cytochrome P450.Conclusion:ADH1A,ADH1C and ADH4 may be biomarkers for the prognosis of HCC,providing reference value for the practical application of ADHs in HCC.
基金Wuhan Science and Technology project(201161038342-02)
文摘Objective:To analyze the differential expression and function prediction of miRNAs in Hepatocellular carcinoma (HCC) based on Bioinformatics, and to provide new ideas for early diagnosis and late intervention of HCC.Methods: The expression dataset of HCC non-coding RNA was obtained by GEO database, and differential miRNAs were screened and visualized by limma R package and pheatmap R package using R (v 3.6.0). Transcriptional factors of differential miRNAs were predicted, signaling pathway analysis and GO enrichment analysis were performed and visualized.Results: A total of 37 miRNAs were selected in HCC tissues with differential expressions, and the significant transcription factors were EGR1, POU2F1, SP1, NKX6-1, MEF2A, FOXA1, SP4, NFIC, RREB1, ARID3A, HOXA5, HNF4A, HOXD8, RORA, LHX3, POU3F2, FOXJ2, POU5F1, NKX2-1, SOX1. Differential miRNAs were found to be mainly located in the Nucleus and Cytoplasm, It has molecular functions such as Protein serine/threonine kinase activity, Receptor signaling complex scaffold activity, Transcription regulator activity and Transcription factor activity, Involved in Nucleic acid metabolism and Signal transduction and Cell communication. Through The epidermal growth factor receptor (EGFR), IGF-1,γ-IFN, Plasma membrane estrogen receptor signaling pathways play a role, and differential miRNAs is associated with VEGF, VEGFR, PDGF, Hepatocyte Growth Factor Receptor (c-Met), Protein serine/threonine kinase mediated signaling events.Conclusion:Differential mirnas in hepatocellular carcinoma are closely related to tumor development and progression, and differential miRNAs can be used as potential markers for early diagnosis of HCC, providing new ideas for late intervention.
