Type 2 diabetes mellitus(T2DM)is a complex metabolic disease threatening human health.We investigated the effects of Tegillarca granosa polysaccharide(TGP)and determined its potential mechanisms in a mouse model of T2...Type 2 diabetes mellitus(T2DM)is a complex metabolic disease threatening human health.We investigated the effects of Tegillarca granosa polysaccharide(TGP)and determined its potential mechanisms in a mouse model of T2DM established through a high-fat diet and streptozotocin.TGP(5.1×10^(3) Da)was composed of mannose,glucosamine,rhamnose,glucuronic acid,galactosamine,glucose,galactose,xylose,and fucose.It could significantly alleviate weight loss,reduce fasting blood glucose levels,reverse dyslipidemia,reduce liver damage from oxidative stress,and improve insulin sensitivity.RT-PCR and Western blotting indicated that TGP could activate the phosphatidylinositol-3-kinase/protein kinase B signaling pathway to regulate disorders in glucolipid metabolism and improve insulin resistance.TGP increased the abundance of Allobaculum,Akkermansia,and Bifidobacterium,restored the microbiota abundance in the intestinal tracts of mice with T2DM,and promoted short-chain fatty acid production.This study provides new insights into the antidiabetic effects of TGP and highlights its potential as a natural hypoglycemic nutraceutical.展开更多
The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregna...The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregnating fresh scallops with 0.2 g/100 m L konjac glucomannan(KGM),carrageenan(CA),and sodium alginate(SA)before the drying and rehydration process.After rehydration,the KGM treated samples had better water retention and the highest betaine content.The CA treated samples had a deepened yellow color and the highest content of flavoring amino acids(FAA)and inosine monophosphate(IMP).What’s more,a total of 77 volatile compounds were detected,with a maximum of 15 esters.The highest proportion in the four groups was eugenol,followed by n-hexadecanoic acid,methyl hexadecanoate etc.The SA group had no significant contribution in terms of quality and taste.The samples pretreated with KGM had the best quality,and those pretreated by CA had the best flavor.展开更多
Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety.In this study,5 species of bacteria,mainly gram-positive bacteria including three species of Bacillus sp...Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety.In this study,5 species of bacteria,mainly gram-positive bacteria including three species of Bacillus spp.,Arthrobacter arilaitensis and Staphylococcus warneri,were isolated and identifi ed from bacteria-contaminated A.auricular culture bags.An in silico predicted antimicrobial peptide from theβ-1,3-glucan-binding protein sequence of Penaeus vannamei,designated Pv GBP2(FLKLGRKSRYGMLKL),was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp.explored.The minimal inhibitory concentrations(MIC)of Pv GBP2 on Bacillus spp.were 15.6–31.25μg/m L.Peptide Pv GBP2 could inhibit Bacillus subtilis in A.auricular culture bags to maintain growth and yield of A.auricular.Transmission electron microscopy(TEM)revealed that Pv GBP2 kills bacteria by perforating the cell wall,destroying membrane integrity and resulting in the leakage of intracellular solutes.In addition,Pv GBP2 inhibits biofi lm formation by B.subtilis by 90.6%at 1×MIC.Thus,peptide Pv GBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A.auricular cultivation and the spread of foodborne pathogens.展开更多
基金funded by the National Key Research and Development Program of China(2020YFD0900902)Zhejiang Province Public Welfare Technology Application Research Project(LGJ21C20001)Zhejiang Provincial Key Research and Development Project of China(2019C02076 and 2019C02075)。
文摘Type 2 diabetes mellitus(T2DM)is a complex metabolic disease threatening human health.We investigated the effects of Tegillarca granosa polysaccharide(TGP)and determined its potential mechanisms in a mouse model of T2DM established through a high-fat diet and streptozotocin.TGP(5.1×10^(3) Da)was composed of mannose,glucosamine,rhamnose,glucuronic acid,galactosamine,glucose,galactose,xylose,and fucose.It could significantly alleviate weight loss,reduce fasting blood glucose levels,reverse dyslipidemia,reduce liver damage from oxidative stress,and improve insulin sensitivity.RT-PCR and Western blotting indicated that TGP could activate the phosphatidylinositol-3-kinase/protein kinase B signaling pathway to regulate disorders in glucolipid metabolism and improve insulin resistance.TGP increased the abundance of Allobaculum,Akkermansia,and Bifidobacterium,restored the microbiota abundance in the intestinal tracts of mice with T2DM,and promoted short-chain fatty acid production.This study provides new insights into the antidiabetic effects of TGP and highlights its potential as a natural hypoglycemic nutraceutical.
基金funded by the National Key R&D Program of China(2020YFD0900900)。
文摘The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregnating fresh scallops with 0.2 g/100 m L konjac glucomannan(KGM),carrageenan(CA),and sodium alginate(SA)before the drying and rehydration process.After rehydration,the KGM treated samples had better water retention and the highest betaine content.The CA treated samples had a deepened yellow color and the highest content of flavoring amino acids(FAA)and inosine monophosphate(IMP).What’s more,a total of 77 volatile compounds were detected,with a maximum of 15 esters.The highest proportion in the four groups was eugenol,followed by n-hexadecanoic acid,methyl hexadecanoate etc.The SA group had no significant contribution in terms of quality and taste.The samples pretreated with KGM had the best quality,and those pretreated by CA had the best flavor.
基金sponsored by National Key R&D Program of China(2020YFD09009)。
文摘Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety.In this study,5 species of bacteria,mainly gram-positive bacteria including three species of Bacillus spp.,Arthrobacter arilaitensis and Staphylococcus warneri,were isolated and identifi ed from bacteria-contaminated A.auricular culture bags.An in silico predicted antimicrobial peptide from theβ-1,3-glucan-binding protein sequence of Penaeus vannamei,designated Pv GBP2(FLKLGRKSRYGMLKL),was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp.explored.The minimal inhibitory concentrations(MIC)of Pv GBP2 on Bacillus spp.were 15.6–31.25μg/m L.Peptide Pv GBP2 could inhibit Bacillus subtilis in A.auricular culture bags to maintain growth and yield of A.auricular.Transmission electron microscopy(TEM)revealed that Pv GBP2 kills bacteria by perforating the cell wall,destroying membrane integrity and resulting in the leakage of intracellular solutes.In addition,Pv GBP2 inhibits biofi lm formation by B.subtilis by 90.6%at 1×MIC.Thus,peptide Pv GBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A.auricular cultivation and the spread of foodborne pathogens.
