A new dye-staining method for protein assay is described.The reactionbetween TPPS<sub>4</sub> and protein molecule causes a shift in the absorption of TPPS<sub>4</sub>from 435 nm to 488nm.The a...A new dye-staining method for protein assay is described.The reactionbetween TPPS<sub>4</sub> and protein molecule causes a shift in the absorption of TPPS<sub>4</sub>from 435 nm to 488nm.The absorbance at 488 nm is proportional to theconcentration of protein.The range of Beer’s law was 1-5 ug/ml and the Sandell’ssensitivity was 0.0087 ug/cm<sup>2</sup>.展开更多
文摘A new dye-staining method for protein assay is described.The reactionbetween TPPS<sub>4</sub> and protein molecule causes a shift in the absorption of TPPS<sub>4</sub>from 435 nm to 488nm.The absorbance at 488 nm is proportional to theconcentration of protein.The range of Beer’s law was 1-5 ug/ml and the Sandell’ssensitivity was 0.0087 ug/cm<sup>2</sup>.