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A NEW STAINING METHOD FOR THE MEASUREMENT OF PROTEIN USING TETRAPHENYLPORPHYRIN TETRASULFONATE(TPPS_4)
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作者 shen yang tong na li chemistry department,peking university,beijing,100871 《Chinese Chemical Letters》 SCIE CAS CSCD 1993年第12期1079-1080,共2页
A new dye-staining method for protein assay is described.The reactionbetween TPPS<sub>4</sub> and protein molecule causes a shift in the absorption of TPPS<sub>4</sub>from 435 nm to 488nm.The a... A new dye-staining method for protein assay is described.The reactionbetween TPPS<sub>4</sub> and protein molecule causes a shift in the absorption of TPPS<sub>4</sub>from 435 nm to 488nm.The absorbance at 488 nm is proportional to theconcentration of protein.The range of Beer’s law was 1-5 ug/ml and the Sandell’ssensitivity was 0.0087 ug/cm<sup>2</sup>. 展开更多
关键词 ABSORBANCE STAINING proportional blank DILUTED herein BRADFORD bovine deter IgG
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