In molecular modeling of electrical double layers(EDLs),the constant charge method(CCM)is prized for its computational efficiency but cannot maintain electrode equipotentiality like the more resourceintensive constant...In molecular modeling of electrical double layers(EDLs),the constant charge method(CCM)is prized for its computational efficiency but cannot maintain electrode equipotentiality like the more resourceintensive constant potential method(CPM),potentially leading to inaccuracies.In certain scenarios,CCM can yield results identical to CPM.However,there are no clear guidelines to determine when CCM is sufficient and when CPM is required.Here,we conduct a series of molecular simulations across various electrodes and electrolytes to present a comprehensive comparison between CCM and CPM under different charging modes.Results reveal that CCM approximates CPM effectively in capturing equilibrium EDL and current-driven dynamics in open electrode systems featuring ionic liquids or regular concentration aqueous electrolytes,while CPM is indispensable in scenarios involving organic and highly concentrated aqueous electrolytes,nanoconfinement effects,and voltage-driven dynamics.This work helps to select appropriate methods for modeling EDL systems,prioritizing accuracy while considering computationalefficiency.展开更多
Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neur...Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neuronal death and infiltration of inflammatory cells, exerting a neuroprotective effect. We hypothesized that the combination of mild therapeutic hypothermia and adipose-derived stem cells would be neuroprotective for treatment of stroke. A rat model of transient middle cerebral artery occlusion was established using the nylon monofilament method. Mild therapeutic hypothermia（33°C） was induced after 2 hours of ischemia. Adipose-derived stem cells were administered through the femoral vein during reperfusion. The severity of neurological dysfunction was measured by a modified Neurological Severity Score Scaling System. The area of the infarct lesion was determined by 2,3,5-triphenyltetrazolium chloride staining. Apoptotic neurons were detected by terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling（TUNEL） staining. The regeneration of microvessels and changes in the glial scar were detected by immunofluorescence staining. The inflammatory responses after ischemic brain injury were evaluated by in situ staining using markers of inflammatory cells. The expression of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction. Compared with mild therapeutic hypothermia or adipose-derived stem cell treatment alone, their combination substantially improved neurological deficits and decreased infarct size. They synergistically reduced the number of TUNEL-positive cells and glial fibrillary acidic protein expression, increased vascular endothelial growth factor levels, effectively reduced inflammatory cell infiltration and down-regulated the m RNA expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α and interleukin-6. Our findings indicate that combined treatment is a better approach for treating stroke compared with mild therapeutic hypothermia or adipose-derived stem cells alone.展开更多
Objective Previous studies showed that hypoxia preconditioning could protect cardiac function against subsequent myo-cardial infarction injury. However, the effect of hypoxia on left ventricular after myocardial infar...Objective Previous studies showed that hypoxia preconditioning could protect cardiac function against subsequent myo-cardial infarction injury. However, the effect of hypoxia on left ventricular after myocardial infarction is still unclear. This study therefore aims to investigate the effects of hypoxia training on left ventricular remodeling in rabbits post myocardial infarction. Methods Adult male rabbits were randomly divided into three groups： group SO （sham operated）, group MI （myocardial infarc-tion only） and group MI-HT （myocardial infarction plus hypoxia training）. Myocardial infarction was induced by left ventricular branch ligation. Hypoxia training was performed in a hypobaric chamber （having equivalent condition at an altitude of 4000 m, FiO214.9%） for 1 h/day, 5 days/week for four weeks. At the endpoints, vascular endothelial growth factor （VEGF） in the plasma was measured. Infarct size and capillary density were detected by histology. Left ventricular remodeling and function were as-sessed by echocardiography.Results After the 4-week experiment, compared with the group SO, plasma VEGF levels in groups MI （130.27 ± 18.58 pg/mL,P〈 0.01） and MI-HT （181.93 ± 20.29 pg/mL,P〈 0.01） were significantly increased. Infarct size in Group MI-HT （29.67% ± 7.73%） was deceased remarkably, while its capillary density （816.0 ± 122.2/mm2） was significantly increased. For both groups MI and MI-HT, left ventricular end-diastolic and end-systolic dimensions were increased whereas left ventricular ejection fraction was decreased. However, compared with group MI, group MI-HT diminished left ventricular end-diastolic （15.86 ± 1.09 mm,P〈 0.05） and end-systolic dimensions （12.10 ± 1.20 mm,P〈 0.01） significantly and im-proved left ventricular ejection fraction （54.39 ± 12.74 mm,P〈 0.05）.ConclusionHypoxia training may improve left ven-tricular function and reduce remodeling via angiogenesis in rabbits with MI.展开更多
Objective To investigate the clinical application value of Loewenstein Occupational Therapy Cognitive Assessment （LOTCA） battery in Chinese patients with post-stroke aphasia. Methods Cognitive fimctions of 59 Chine...Objective To investigate the clinical application value of Loewenstein Occupational Therapy Cognitive Assessment （LOTCA） battery in Chinese patients with post-stroke aphasia. Methods Cognitive fimctions of 59 Chinese patients with aphasia following a stroke were assessed with the Chinese version of the second edition of LOTCA battery and their linguistic functions were tested with the Western Aphasia Battery （WAB） Scale, respectively. The results of LOTCA were analyzed and compared across different groups, in the light of gender, age, educational background, the length of illness, and the degree of aphasia.展开更多
The mechanism underlying body weight support treadmill training in elderly hemiplegic stroke patients is largely unknown. This study aimed to elucidate the changes of cortical blood flow in seven elderly patients with...The mechanism underlying body weight support treadmill training in elderly hemiplegic stroke patients is largely unknown. This study aimed to elucidate the changes of cortical blood flow in seven elderly patients with post-stroke hemiplegia before and after body weight support treadmill training by semi-quantitative analysis of regional cerebral blood flow assessed by single photon emission computed tomography. Body weight support treadmill training for 6 months was effective in improving cerebral blood flow and promoting the walking speed and balance recovery in elderly patients with post-stroke hemiplegia.展开更多
In vitro studies have shown that estrogen receptor β can attenuate the cytotoxic effect of amyloid protein on PC12 cells through the Akt pathway without estrogen stimulation. In this study, we aimed to observe the ef...In vitro studies have shown that estrogen receptor β can attenuate the cytotoxic effect of amyloid protein on PC12 cells through the Akt pathway without estrogen stimulation. In this study, we aimed to observe the effect of estrogen receptor β in Alzheimer＇s disease rat models established by intraventricular injection of amyloid β protein. Estrogen receptor β lentiviral particles delivered via intraventricular injection increased Akt content in the hippocampus, decreased interleukin-1β mRNA tumor necrosis factor a mRNA and amyloid β protein levels in the hippocampus, and improved the learning and memory capacities in AIzheimer＇s disease rats. Estrogen receptor β short hairpin RNA lentiviral particles delivered via intraventricular injection had none of the above impacts on AIzheimer＇s disease rats. These experimental findings indicate that estrogen receptor β, independent from estrogen, can reduce inflammatory reactions and amyloid β deposition in the hJppocampus of AIzheimer＇s disease rats, and improve learning and memory capacities. This effect may be mediated through activation of the Akt pathway.展开更多
The mixed teaching mode plays an increasingly important role in stimulating students’interest and autonomy in learning,and strengthening students’learning ability.The full integration of mixed teaching mode and micr...The mixed teaching mode plays an increasingly important role in stimulating students’interest and autonomy in learning,and strengthening students’learning ability.The full integration of mixed teaching mode and microelectronics teaching can not only achieve the teaching objectives smoothly,but also enable students to deepen their understanding and memory of relevant knowledge with the help of diversified and interesting teaching methods.Therefore,this paper takes the microelectronics course as an example to practice and explore the effective ways to carry out the mixed teaching mode.Teachers should not make full use of online and offline teaching resources,but also actively improve the traditional assessment systems.Through the continuous improvement of the practicality of online and offline teaching content,an easy-to-complex teaching method with a coherent content structure can be adopted to stimulate students’learning motivation,improve their enthusiasm for participation,and lay a solid foundation for further improvements in the teaching of microelectronics technology.展开更多
With the construction of smart grid,lots of renewable energy resources such as wind and solar are deployed in power system.It might make the power system load varied complex than before which will bring difficulties i...With the construction of smart grid,lots of renewable energy resources such as wind and solar are deployed in power system.It might make the power system load varied complex than before which will bring difficulties in short-term load forecasting area.To overcome this issue,this paper proposes a new short-term load forecasting framework based on big data technologies.First,a cluster analysis is performed to classify daily load patterns for individual loads using smart meter data.Next,an association analysis is used to determine critical influential factors.This is followed by the application of a decision tree to establish classification rules.Then,appropriate forecasting models are chosen for different load patterns.Finally,the forecasted total system load is obtained through an aggregation of an individual load’s forecasting results.Case studies using real load data show that the proposed new framework can guarantee the accuracy of short-term load forecasting within required limits.展开更多
As the basis of machine vision,the biomimetic image sensing devices are the eyes of artificial intelligence.In recent years,with the development of two-dimensional(2D)materials,many new optoelectronic devices are deve...As the basis of machine vision,the biomimetic image sensing devices are the eyes of artificial intelligence.In recent years,with the development of two-dimensional(2D)materials,many new optoelectronic devices are developed for their outstanding performance.However,there are still little sensing arrays based on 2D materials with high imaging quality,due to the poor uniformity of pixels caused by material defects and fabrication technique.Here,we propose a 2D MoS_(2)sensing array based on artificial neural network(ANN)learning.By equipping the MoS_(2)sensing array with a“brain”(ANN),the imaging quality can be effectively improved.In the test,the relative standard deviation(RSD)between pixels decreased from about 34.3%to 6.2%and 5.49%after adjustment by the back propagation(BP)and Elman neural networks,respectively.The peak signal to noise ratio(PSNR)and structural similarity(SSIM)of the image are improved by about 2.5 times,which realizes the re-recognition of the distorted image.This provides a feasible approach for the application of 2D sensing array by integrating ANN to achieve high quality imaging.展开更多
The Multi-receiver Encryption(MRE)scheme can meet the secure data transmission requirements in multicast and broadcast scenarios.To meet compliance,critical information infrastructure in China should be protected with...The Multi-receiver Encryption(MRE)scheme can meet the secure data transmission requirements in multicast and broadcast scenarios.To meet compliance,critical information infrastructure in China should be protected with Chinese national commercial cryptographic algorithms.Designing an MRE scheme based on Elliptic Curve Cryptography(ECC)is one of the current design methods with better flexibility and performance.However,the research on MRE schemes based on SM2 elliptic curve public-key cryptography is still in a blank state.This paper proposes a Certificateless SM2-based Multireceiver Encryption(CL-SM2-MRE)scheme.We prove the security of the CL-SM2-MRE scheme under the Random Oracle Model(ROM)and analyze the performance.展开更多
基金the funding support from the National Natural Science Foundation of China(T2325012 and 52161135104)the Program for HUST Academic Frontier Youth Team.
文摘In molecular modeling of electrical double layers(EDLs),the constant charge method(CCM)is prized for its computational efficiency but cannot maintain electrode equipotentiality like the more resourceintensive constant potential method(CPM),potentially leading to inaccuracies.In certain scenarios,CCM can yield results identical to CPM.However,there are no clear guidelines to determine when CCM is sufficient and when CPM is required.Here,we conduct a series of molecular simulations across various electrodes and electrolytes to present a comprehensive comparison between CCM and CPM under different charging modes.Results reveal that CCM approximates CPM effectively in capturing equilibrium EDL and current-driven dynamics in open electrode systems featuring ionic liquids or regular concentration aqueous electrolytes,while CPM is indispensable in scenarios involving organic and highly concentrated aqueous electrolytes,nanoconfinement effects,and voltage-driven dynamics.This work helps to select appropriate methods for modeling EDL systems,prioritizing accuracy while considering computationalefficiency.
基金supported by the National Natural Science Foundation of China,No.81371301
文摘Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neuronal death and infiltration of inflammatory cells, exerting a neuroprotective effect. We hypothesized that the combination of mild therapeutic hypothermia and adipose-derived stem cells would be neuroprotective for treatment of stroke. A rat model of transient middle cerebral artery occlusion was established using the nylon monofilament method. Mild therapeutic hypothermia（33°C） was induced after 2 hours of ischemia. Adipose-derived stem cells were administered through the femoral vein during reperfusion. The severity of neurological dysfunction was measured by a modified Neurological Severity Score Scaling System. The area of the infarct lesion was determined by 2,3,5-triphenyltetrazolium chloride staining. Apoptotic neurons were detected by terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling（TUNEL） staining. The regeneration of microvessels and changes in the glial scar were detected by immunofluorescence staining. The inflammatory responses after ischemic brain injury were evaluated by in situ staining using markers of inflammatory cells. The expression of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction. Compared with mild therapeutic hypothermia or adipose-derived stem cell treatment alone, their combination substantially improved neurological deficits and decreased infarct size. They synergistically reduced the number of TUNEL-positive cells and glial fibrillary acidic protein expression, increased vascular endothelial growth factor levels, effectively reduced inflammatory cell infiltration and down-regulated the m RNA expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α and interleukin-6. Our findings indicate that combined treatment is a better approach for treating stroke compared with mild therapeutic hypothermia or adipose-derived stem cells alone.
基金We are grateful to the support of Dr. Lei Yuan and Shao-Shao Zhao for their technical assistance. This work was supported in part by China Postdoctoral Science Foundation Province, China
文摘Objective Previous studies showed that hypoxia preconditioning could protect cardiac function against subsequent myo-cardial infarction injury. However, the effect of hypoxia on left ventricular after myocardial infarction is still unclear. This study therefore aims to investigate the effects of hypoxia training on left ventricular remodeling in rabbits post myocardial infarction. Methods Adult male rabbits were randomly divided into three groups： group SO （sham operated）, group MI （myocardial infarc-tion only） and group MI-HT （myocardial infarction plus hypoxia training）. Myocardial infarction was induced by left ventricular branch ligation. Hypoxia training was performed in a hypobaric chamber （having equivalent condition at an altitude of 4000 m, FiO214.9%） for 1 h/day, 5 days/week for four weeks. At the endpoints, vascular endothelial growth factor （VEGF） in the plasma was measured. Infarct size and capillary density were detected by histology. Left ventricular remodeling and function were as-sessed by echocardiography.Results After the 4-week experiment, compared with the group SO, plasma VEGF levels in groups MI （130.27 ± 18.58 pg/mL,P〈 0.01） and MI-HT （181.93 ± 20.29 pg/mL,P〈 0.01） were significantly increased. Infarct size in Group MI-HT （29.67% ± 7.73%） was deceased remarkably, while its capillary density （816.0 ± 122.2/mm2） was significantly increased. For both groups MI and MI-HT, left ventricular end-diastolic and end-systolic dimensions were increased whereas left ventricular ejection fraction was decreased. However, compared with group MI, group MI-HT diminished left ventricular end-diastolic （15.86 ± 1.09 mm,P〈 0.05） and end-systolic dimensions （12.10 ± 1.20 mm,P〈 0.01） significantly and im-proved left ventricular ejection fraction （54.39 ± 12.74 mm,P〈 0.05）.ConclusionHypoxia training may improve left ven-tricular function and reduce remodeling via angiogenesis in rabbits with MI.
文摘Objective To investigate the clinical application value of Loewenstein Occupational Therapy Cognitive Assessment （LOTCA） battery in Chinese patients with post-stroke aphasia. Methods Cognitive fimctions of 59 Chinese patients with aphasia following a stroke were assessed with the Chinese version of the second edition of LOTCA battery and their linguistic functions were tested with the Western Aphasia Battery （WAB） Scale, respectively. The results of LOTCA were analyzed and compared across different groups, in the light of gender, age, educational background, the length of illness, and the degree of aphasia.
文摘The mechanism underlying body weight support treadmill training in elderly hemiplegic stroke patients is largely unknown. This study aimed to elucidate the changes of cortical blood flow in seven elderly patients with post-stroke hemiplegia before and after body weight support treadmill training by semi-quantitative analysis of regional cerebral blood flow assessed by single photon emission computed tomography. Body weight support treadmill training for 6 months was effective in improving cerebral blood flow and promoting the walking speed and balance recovery in elderly patients with post-stroke hemiplegia.
文摘In vitro studies have shown that estrogen receptor β can attenuate the cytotoxic effect of amyloid protein on PC12 cells through the Akt pathway without estrogen stimulation. In this study, we aimed to observe the effect of estrogen receptor β in Alzheimer＇s disease rat models established by intraventricular injection of amyloid β protein. Estrogen receptor β lentiviral particles delivered via intraventricular injection increased Akt content in the hippocampus, decreased interleukin-1β mRNA tumor necrosis factor a mRNA and amyloid β protein levels in the hippocampus, and improved the learning and memory capacities in AIzheimer＇s disease rats. Estrogen receptor β short hairpin RNA lentiviral particles delivered via intraventricular injection had none of the above impacts on AIzheimer＇s disease rats. These experimental findings indicate that estrogen receptor β, independent from estrogen, can reduce inflammatory reactions and amyloid β deposition in the hJppocampus of AIzheimer＇s disease rats, and improve learning and memory capacities. This effect may be mediated through activation of the Akt pathway.
文摘The mixed teaching mode plays an increasingly important role in stimulating students’interest and autonomy in learning,and strengthening students’learning ability.The full integration of mixed teaching mode and microelectronics teaching can not only achieve the teaching objectives smoothly,but also enable students to deepen their understanding and memory of relevant knowledge with the help of diversified and interesting teaching methods.Therefore,this paper takes the microelectronics course as an example to practice and explore the effective ways to carry out the mixed teaching mode.Teachers should not make full use of online and offline teaching resources,but also actively improve the traditional assessment systems.Through the continuous improvement of the practicality of online and offline teaching content,an easy-to-complex teaching method with a coherent content structure can be adopted to stimulate students’learning motivation,improve their enthusiasm for participation,and lay a solid foundation for further improvements in the teaching of microelectronics technology.
文摘With the construction of smart grid,lots of renewable energy resources such as wind and solar are deployed in power system.It might make the power system load varied complex than before which will bring difficulties in short-term load forecasting area.To overcome this issue,this paper proposes a new short-term load forecasting framework based on big data technologies.First,a cluster analysis is performed to classify daily load patterns for individual loads using smart meter data.Next,an association analysis is used to determine critical influential factors.This is followed by the application of a decision tree to establish classification rules.Then,appropriate forecasting models are chosen for different load patterns.Finally,the forecasted total system load is obtained through an aggregation of an individual load’s forecasting results.Case studies using real load data show that the proposed new framework can guarantee the accuracy of short-term load forecasting within required limits.
基金This project was financially supported by the Dalian Science and Technology Innovation Fund of China(No.2019J11CY011)the Science Fund for Creative Research Groups of NSFC(No.51621064).
文摘As the basis of machine vision,the biomimetic image sensing devices are the eyes of artificial intelligence.In recent years,with the development of two-dimensional(2D)materials,many new optoelectronic devices are developed for their outstanding performance.However,there are still little sensing arrays based on 2D materials with high imaging quality,due to the poor uniformity of pixels caused by material defects and fabrication technique.Here,we propose a 2D MoS_(2)sensing array based on artificial neural network(ANN)learning.By equipping the MoS_(2)sensing array with a“brain”(ANN),the imaging quality can be effectively improved.In the test,the relative standard deviation(RSD)between pixels decreased from about 34.3%to 6.2%and 5.49%after adjustment by the back propagation(BP)and Elman neural networks,respectively.The peak signal to noise ratio(PSNR)and structural similarity(SSIM)of the image are improved by about 2.5 times,which realizes the re-recognition of the distorted image.This provides a feasible approach for the application of 2D sensing array by integrating ANN to achieve high quality imaging.
基金The work was supported by the National Key Research and Development Program of China(2021YFA1000600)the National Natural Science Foundation of China(U21A20466,62272350,U1865202)+3 种基金the Special Project on Science and Technology Program of Hubei Province,China(2020AEA013,2021BAA025)the Fok Ying Tung Foundation,China(171057)the National Cryptography Development Fund,China(MMJJ20180105)the Fundamental Research Funds for Central Public Welfare Research Institutes of China(CKSF2019526/TG3).
文摘The Multi-receiver Encryption(MRE)scheme can meet the secure data transmission requirements in multicast and broadcast scenarios.To meet compliance,critical information infrastructure in China should be protected with Chinese national commercial cryptographic algorithms.Designing an MRE scheme based on Elliptic Curve Cryptography(ECC)is one of the current design methods with better flexibility and performance.However,the research on MRE schemes based on SM2 elliptic curve public-key cryptography is still in a blank state.This paper proposes a Certificateless SM2-based Multireceiver Encryption(CL-SM2-MRE)scheme.We prove the security of the CL-SM2-MRE scheme under the Random Oracle Model(ROM)and analyze the performance.
