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伏杀硫磷对斑马鱼心脏发育毒性及氧化损伤机制 被引量:2
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作者 盛众 陈家乐 +3 位作者 王斌杰 吴元钊 姚伟宣 王继业 《中国环境科学》 EI CAS CSCD 北大核心 2023年第4期1957-1966,共10页
以斑马鱼为研究对象,评价伏杀硫磷急性暴露对幼鱼心脏发育毒性及氧化损伤作用.结果表明,1和3mg/L的伏杀硫磷导致斑马鱼胚胎孵化率分别下降10.37%和22.96%,体长分别缩短11.77%和19.40%,心率显著下降,产生心包水肿等胚胎发育畸形.抗氧化酶... 以斑马鱼为研究对象,评价伏杀硫磷急性暴露对幼鱼心脏发育毒性及氧化损伤作用.结果表明,1和3mg/L的伏杀硫磷导致斑马鱼胚胎孵化率分别下降10.37%和22.96%,体长分别缩短11.77%和19.40%,心率显著下降,产生心包水肿等胚胎发育畸形.抗氧化酶(SOD,CAT)活性检测显示伏杀硫磷造成斑马鱼幼鱼体内SOD活性随浓度增加先上升后下降,CAT活性显著下降;活性氧(ROS)检测表明伏杀硫磷对幼鱼的心脏部位造成氧化损伤;Tg(cmlc2:EGFP)转基因斑马鱼证明伏杀硫磷诱导幼鱼的心房和心室过度分离;吖啶橙(AO)染色显示幼鱼心脏部位发生细胞过度凋亡;心脏发育相关的基因中nppa和sox9b显著上调,gata4和vmhc显著下调;凋亡相关基因bcl2、bax、Puma、Mdm2的表达均显著下调.伏杀硫磷暴露造成的心脏发育毒性与氧化损伤和细胞凋亡平衡的破坏相关. 展开更多
关键词 伏杀硫磷 斑马鱼 氧化损伤 心脏毒性
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新型分子检测技术在基层实验室诊断肺结核的效能分析 被引量:14
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作者 彭英 苏欣 +5 位作者 江琦 陈昌明 卞然 钟声 高谦 李发滨 《中国防痨杂志》 CAS CSCD 2019年第2期145-148,共4页
目的分析与比较GeneXpert MTB/RIF(简称“GeneXpert”)与交叉引物核酸恒温扩增(cross-priming amplification,CPA)技术在基层实验室结核病快速诊断中的效能。方法连续收集黑龙江省五常市结核病防治所2014年4月至2017年7月初诊肺结核可... 目的分析与比较GeneXpert MTB/RIF(简称“GeneXpert”)与交叉引物核酸恒温扩增(cross-priming amplification,CPA)技术在基层实验室结核病快速诊断中的效能。方法连续收集黑龙江省五常市结核病防治所2014年4月至2017年7月初诊肺结核可疑患者的痰标本,分别进行改良罗氏固体培养基培养法(简称“固体培养法”)与快速分子检测(A组采用GeneXpert检测了787例;B组采用CPA检测了501例)。以固体培养法为标准,计算2种分子检测技术的敏感度、特异度、Kappa值。结果A组共检测787例患者痰标本,其中培养阳性229例,GeneXpert阳性300例,GeneXpert检测的敏感度、特异度分别为99.1%(227/229)、86.9%(485/558),Kappa=0.788。B组共检测501例患者痰标本,其中培养阳性129例,CPA检测阳性125例。CPA检测的敏感度、特异度分别为81.4%(105/129)、94.6%(352/372),Kappa=0.768。2种分子检测技术与固体培养法检测结果的一致率比较,差异无统计学意义[90.5%(712/787)vs91.2%(457/501);χ^2=0.204,P=0.652)]。结论CPA检测的准确性与GeneXpert相当,但其成本低、无需检测仪器、允许每批次大量样本同时操作,更适用于基层实验室对肺结核患者的早期诊断。 展开更多
关键词 分枝杆菌 结核 分子探针技术 核酸扩增技术 实验室技术和方法 对比研究
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Effect of Thermal Cycling on Properties and Microstructure of SnAgCuCe Soldered Joints in QFP Devices 被引量:4
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作者 ZHANG Liang XUE Songbai +3 位作者 GAO Lili ZENG Guang YU shenglin sheng zhong 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2011年第4期561-566,共6页
Increasing global concern about the environment is bringing regulatory (European directives) and consumer ("green products") pressure on the electronics industry in Europe and Japan to reduce or completely elimi... Increasing global concern about the environment is bringing regulatory (European directives) and consumer ("green products") pressure on the electronics industry in Europe and Japan to reduce or completely eliminate the use of lead (Pb) in products. Among all lead-free solder alloys, SnAgCu solder system, which has better thermo-mechanical properties compared with those of SnPb solder, is proven to be one of the promising candidates for electronic assembly. Previous work also revealed that adding a small amount of rare earth Ce into SnAgCu solder can visibly improve the properties and inhibit the excessive growth of the intermetallic compound layer. Thermal fatigue properties of SnAgCuCe soldered joints in QFP devices under thermal conditions have been investigated by finite element method and experiments. Based on creep model of low stress and high stress, corresponding creep subroutine was established for simulating the stress and strain response of SnAgCuCe soldered joint from -55 ℃ to 125 ℃, and fatigue life was calculated using creep fatigue life prediction equation. Moreover, thermal cycling experiments were conducted, the experimental results were found to be close to the simulated results. In addition, the tensile force of SnAgCuCe soldered joints decreased with increasing number of thermal cycles, and the fracture mechanism transformed from toughness fracture to brittle intergranular fracture. Moreover the tensile force changes and fracture microstructure evolution could benefit the quantitative evaluations of the mechanical performances of lead-free soldered joints under thermal cycling loadings. 展开更多
关键词 finite element method creep equation thermal cycling fatigue life
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Anand Parameters Determination for SnAgCu Solder Bearing Micro-amounts Rare Earth Ce 被引量:2
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作者 ZHANG Liang XUE Songbai +3 位作者 GAO Lili CHEN Yan YU shenglin sheng zhong 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2010年第2期257-262,共6页
SnAgCu solder system with the addition of rare earth Ce, which has better thermo-mechanical properties compared to those of SnPb solder, is regarded as one of the promising candidates for electronic assembly. Moreover... SnAgCu solder system with the addition of rare earth Ce, which has better thermo-mechanical properties compared to those of SnPb solder, is regarded as one of the promising candidates for electronic assembly. Moreover, the SnAgCuCe solder alloys can provide good quality joints with Cu substrates. However, there is few report of the constitutive model for SnAgCu solder bearing micro-amounts rare earth Ce. In this paper, the unified viscoplastic constitutive model, Anand equations, is used to represent the inelastic deformation behavior for SnAgCu and SnAgCuCe solders. In order to obtain the acquired data for the fitting of the material parameters of this unified model, a series of experiments of constant strain rate test were conducted under isothermal conditions at different temperatures. The Anand parameters of the constitutive equations for SnAgCu and SnAgCuCe solder were determined from separated constitutive relations and experimental results. Nonlinear least-square fitting was selected to determine the model constants. And the simulated results were then compared with experimental measurements of the stress-inelastic strain curves: excellent agreement was found. The model accurately predicted the overall trend of steady-state stress-strain behavior of SnAgCu and SnAgCuCe solders for the temperature ranges from 25℃ to 150 ℃, and the strain rate ranges from 0.01 s^-1 to 0.001 s ^-1. It is concluded that the Anand model can be applied for representing the inelastic deformation behavior of solders at high homologous temperature and can be recommended for finite element simulation of the stress-strain response of lead free soldered joints. Based on the Anand model, the investigations of thermo-mechanical of SnAgCu and SnAgCuCe soldered joints in fine pitch quad flat package by finite element code were done under thermal cyclic loading, it is found that the reliability of SnAgCu soldered joints can be improved remarkably with addition of rare earth Ce. The results may provide a theory guide for developing constitutive model for lead-free solders. 展开更多
关键词 Anand equations constitutive relations SnAgCuCe solders rare earth reliability
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周扫像移补偿大面阵红外光学系统 被引量:1
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作者 刘玉环 周军 +2 位作者 盛忠 李娟 熊健 《电光与控制》 CSCD 北大核心 2022年第8期84-87,共4页
红外搜索与跟踪(IRST)系统在态势感知、环境安全监测等领域具有重要地位。像移补偿是IRST进行快速扫描的关键技术之一。设计了一种长积分时间快速周扫下像移补偿大面阵IRST光学系统,该光学系统采用平行光路稳像方法,具有4 ms级的积分时... 红外搜索与跟踪(IRST)系统在态势感知、环境安全监测等领域具有重要地位。像移补偿是IRST进行快速扫描的关键技术之一。设计了一种长积分时间快速周扫下像移补偿大面阵IRST光学系统,该光学系统采用平行光路稳像方法,具有4 ms级的积分时间、360(°)/s级的周扫速度、1k级的面阵规模等技术特点。介绍了平行光路稳像的原理,提出了参数计算及匹配关系。光学系统设计结果以及光学样机周扫成像测试结果表明,该像移补偿IRST光学系统成像探测能力优良,相关指标处于国内领先水平。 展开更多
关键词 像移补偿 光学设计 长积分时间 快速周扫 大面阵
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Contribution Invitation of The Chinese-German Journal of Clinical Oncology 被引量:1
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作者 sheng zhong Yongjian XU Zhenxiang ZHANG 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第3期177-178,共2页
The Chinese-German Journal of Clinical Oncology is aninternational professional academiic periodical on on-cology,being co-edited by China and Germany.The Journal,with the authors from around world,especially from Chi... The Chinese-German Journal of Clinical Oncology is aninternational professional academiic periodical on on-cology,being co-edited by China and Germany.The Journal,with the authors from around world,especially from China,isdominated in introducing the clinical experience of diagnosisand treatment as well as leading scientific research achieve-ment in the tumor domain,in addition to report basic theoryresearches which help instruct the clinical practice of oncolo-gy and closely connect with the discipline.All the manu-scripts are published in English,quarterly issued both inter-nal and external,64-80 pages,big 16 opens domains,artpaper in offset printing,with lay-out by international cus-toms,unified issuing number:ISSN 0178-3351/CN/42-1654/R. 展开更多
关键词 《中德临床肿瘤学杂志》 稿件 编写格式 投稿要求
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Knowledge Graph Representation Reasoning for Recommendation System 被引量:2
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作者 Tao Li Hao Li +4 位作者 sheng zhong Yan Kang Yachuan Zhang Rongjing Bu Yang Hu 《Journal of New Media》 2020年第1期21-30,共10页
In view of the low interpretability of existing collaborative filtering recommendation algorithms and the difficulty of extracting information from content-based recommendation algorithms,we propose an efficient KGRS ... In view of the low interpretability of existing collaborative filtering recommendation algorithms and the difficulty of extracting information from content-based recommendation algorithms,we propose an efficient KGRS model.KGRS first obtains reasoning paths of knowledge graph and embeds the entities of paths into vectors based on knowledge representation learning TransD algorithm,then uses LSTM and soft attention mechanism to capture the semantic of each path reasoning,then uses convolution operation and pooling operation to distinguish the importance of different paths reasoning.Finally,through the full connection layer and sigmoid function to get the prediction ratings,and the items are sorted according to the prediction ratings to get the user’s recommendation list.KGRS is tested on the movielens-100k dataset.Compared with the related representative algorithm,including the state-of-the-art interpretable recommendation models RKGE and RippleNet,the experimental results show that KGRS has good recommendation interpretation and higher recommendation accuracy. 展开更多
关键词 Knowledge graph collaborative filtering deep learning interpretable recommendation knowledge representation learning
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Contribution Invitation of The Chinese-German Journal of Clinical Oncology
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作者 sheng zhong Yongjian XU Zhenxiang ZHANG 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第1期52-54,共3页
《中德临床肿瘤学杂志》为中德合办的国际性肿瘤专业学术刊物。主要报道世界各国作者,特别是中国作者在肿瘤领域领先的科研成果和临床诊疗经验,以及对肿瘤临床有指导作用、且与肿瘤临床密切结合的基础理论研究。来稿全文以英语发表,... 《中德临床肿瘤学杂志》为中德合办的国际性肿瘤专业学术刊物。主要报道世界各国作者,特别是中国作者在肿瘤领域领先的科研成果和临床诊疗经验,以及对肿瘤临床有指导作用、且与肿瘤临床密切结合的基础理论研究。来稿全文以英语发表,国内外公开发行;季刊,64~80页,大16开,铜版纸印刷胶装,按国际惯例编排;统一刊号:ISSN O178~3351/CN/42-1654/R。 展开更多
关键词 中德临床肿瘤学杂志 肿瘤临床 临床诊疗 稿约 来稿 CN 季刊 编排 专业 刊号
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Contribution Invitation of The Chinese-German Journal of Clinical Oncology
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作者 sheng zhong Yongjian XU and Zhenxiang ZHANG 《The Chinese-German Journal of Clinical Oncology》 CAS 2003年第1期55-56,共2页
关键词 中德临床肿瘤学杂志 征稿启示 论文格式 投稿要求
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Contribution for Invitation for The Chinese-German Journal of Clinical Oncology
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作者 sheng zhong Yongjian XU and Zhenxiang ZHANG 《The Chinese-German Journal of Clinical Oncology》 CAS 2003年第2期118-119,共2页
The Chinese-German Journal of Clinical Oncology isan international, professional, academic periodical on on-cology, co-edited by China and Germany. With the au-thors from around the world, especially from China, thejo... The Chinese-German Journal of Clinical Oncology isan international, professional, academic periodical on on-cology, co-edited by China and Germany. With the au-thors from around the world, especially from China, thejournal focuses on introducing clinical experience in diag-nosis and treatment and leading scientific research a-chievement in the tumor domain, in addition to reportingbasic theory research which help to teach the clinicalpractice of oncology and closely connect with the disci-pline. All manuscripts are published in English: the jour-nal is issued quarterly, with the International SerialStandard Number (ISSN) 1610-1979/CN/42-1654/R. 展开更多
关键词 中德临床肿瘤学杂志 投稿 稿约 稿件格式
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Extension Multi-factorial Evaluation Method on Water Quality Early-warning
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作者 Rong GUO Yong ZHANG +1 位作者 Fangjian XIE sheng zhong 《Asian Agricultural Research》 2013年第6期95-97,共3页
Extension multi-factorial evaluation method was used in water quality early-earning in Yincungang River based on MATLAB. The results showed that water quality in summer was safe,while that in other three seasons were ... Extension multi-factorial evaluation method was used in water quality early-earning in Yincungang River based on MATLAB. The results showed that water quality in summer was safe,while that in other three seasons were in pre-warning state with the order of winter > spring > autumn. 展开更多
关键词 EXTENSION multi-factorial EVALUATION WATER QUALITY
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An Analysis on the Economic Benefit of Simplified Cultivation Technology for Longan in Hills and Mountains of Maoming City
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作者 Jianbin LUO sheng zhong +4 位作者 Jun CHEN Xiangyou ZENG Libin FU Yanzong ZHU Zhenquan DENG 《Asian Agricultural Research》 2016年第11期13-16,共4页
According to the characteristics of longan cultivated in the hill and mountain regions of Maoming City,this paper analyzed the economic benefit of the simplified cultivation techniques. With 15-year-old Chuliang longa... According to the characteristics of longan cultivated in the hill and mountain regions of Maoming City,this paper analyzed the economic benefit of the simplified cultivation techniques. With 15-year-old Chuliang longan trees as materials,the economic benefit of four kinds of simplified cultivation technologies was compared and analyzed. After nine years of continuous technological application,it was found that the input costs increased by 1. 62%,and the output profits increased by 56. 10%; in input costs,labor cost decreased by 41. 33%,pesticide cost decreased by 24. 19%,and fertilizer cost increased by 33. 57%. 展开更多
关键词 Hills and mountains LONGAN Simplified cultivation Economic benefit
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长波红外平面计算衍射光学系统设计
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作者 王喆 盛忠 +4 位作者 韩璟圳 甄政 张承然 马德超 朴明旭 《光学学报》 EI CAS CSCD 北大核心 2024年第14期202-209,共8页
提出一种平面计算衍射光学系统,分析平面衍射光学系统的像差特性,基于波前像差理论并利用Zernike多项式构建波前像差模型,通过傅里叶变换建立点扩散函数模型,结合反卷积算法处理图像,消除了轴外像差对成像的影响。设计一种波段为8~12μ... 提出一种平面计算衍射光学系统,分析平面衍射光学系统的像差特性,基于波前像差理论并利用Zernike多项式构建波前像差模型,通过傅里叶变换建立点扩散函数模型,结合反卷积算法处理图像,消除了轴外像差对成像的影响。设计一种波段为8~12μm、焦距为12 mm、F数为1.2的平面衍射光学系统,利用计算成像的方法将视场从1°扩大到4°,物距为4 m到无限远。结果表明,该方法效果明显,成像质量得到提升,实现了光学系统的小型化和轻量化,为光学系统的集成化提供了新思路。 展开更多
关键词 光学设计 计算成像 衍射元件 点扩散函数 空间变化反卷积
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离子液体电沉积 被引量:1
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作者 钟声 宋婷 +4 位作者 张钰瑞 李垚 赵炜珍 刘瑞霞 张锁江 《中国科学:化学》 CAS CSCD 北大核心 2023年第10期2008-2026,共19页
离子液体具有电化学窗口宽,热稳定性良好和结构性能可调等独特的物理化学性质,是一种有着广阔应用前景的电沉积介质.本文介绍了离子液体电沉积的发展历程、典型的离子液体电沉积体系结构组成及其中的重要科学问题,并综述了近15年来离子... 离子液体具有电化学窗口宽,热稳定性良好和结构性能可调等独特的物理化学性质,是一种有着广阔应用前景的电沉积介质.本文介绍了离子液体电沉积的发展历程、典型的离子液体电沉积体系结构组成及其中的重要科学问题,并综述了近15年来离子液体电沉积的研究进展.本文详细介绍了五种不同类型离子液体中金属/合金的电沉积过程,包括卤素金属酸盐型离子液体、空气和水稳定型离子液体、低共熔溶剂、含金属阳离子型离子液体和质子型离子液体,并且系统概述了调节因素如沉积条件(电流密度、沉积模式、沉积时间、温度)和电解质成分(阴离子、阳离子、金属盐、添加剂、水分)对于沉积物形貌、组成、微观结构和性质的影响规律.之后,重点介绍了离子液体在半导体电沉积中的研究进展.最后,指出了目前离子液体电沉积在基础理论和技术研究中面临的挑战,并展望了其未来在电子信息领域的发展前景. 展开更多
关键词 离子液体 低共熔溶剂 电沉积 金属 半导体
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Insights into the characteristics of corrosion products formed on the contact and exposed regions of C1045 steel bolt and nut fasteners exposed to aqueous chloride environments 被引量:3
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作者 Enobong Felix Daniel Chao Li +6 位作者 Changgang Wang Junhua Dong Inime Ime Udoh Peter Chukwuemeka Okafor Dongjiu Zhang Wenan zhong sheng zhong 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2023年第4期250-264,共15页
This study investigated the characteristics of corrosion products formed on the contact and exposed re-gions of C1045 steel bolt and nut fasteners exposed to aqueous chloride environments.The corroded sur-face morphol... This study investigated the characteristics of corrosion products formed on the contact and exposed re-gions of C1045 steel bolt and nut fasteners exposed to aqueous chloride environments.The corroded sur-face morphology,rust compositions,and corrosion kinetics of the bolt specimen were studied by visual observation,optical microscopy(OM),scanning electron microscopy(SEM),X-Ray diffractometry(XRD),micro-Raman,electron probe micro-analyser(EPMA),and potentiodynamic polarization techniques.Re-sults obtained showed a variation in corrosion kinetics,morphology,and composition of the rust layer which were driven by differential aeration and concentration effects.Due to the availability of sufficient dissolved oxygen,the oxyhydroxide compound,lepidocrocite(γ-FeOOH)was detected in the outer rust layer in the exposed region,whereas the inner rust layer was composed of magnetite(Fe_(3)O_(4)).How-ever,the oxygen-deficient contact surface revealed the presence of akaganeite(β-FeOOH)and magnetite(Fe_(3)O_(4))as dominant oxide phases.The most stable phase,goethite(α-FeOOH)was also detected in the rust formed in both regions,though in significantly low amounts.Furthermore,owing to variation in environmental conditions,the amount and density of the rust layer varied in the different regions.The estimated corrosion stability values for the different regions revealed that the corrosion products formed on the steel surfaces were non-protective,suggesting the need for specific surface treatment as a protec-tive measure. 展开更多
关键词 Carbon steel Bolt-nut fastener Raman SEM-EDS XRD Rust characterization
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Quantitative analysis on the phase margin of ADRC 被引量:1
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作者 sheng zhong Yi Huang 《Control Theory and Technology》 EI CSCD 2023年第1期4-15,共12页
The paper analyzes and compares the phase margins of four active disturbance rejection control(ADRC)designs,which are based on different common-used extended state observers(ESOs)of various orders,for second-order sys... The paper analyzes and compares the phase margins of four active disturbance rejection control(ADRC)designs,which are based on different common-used extended state observers(ESOs)of various orders,for second-order systems.The quantitative results indicate that,besides good dynamic response,ADRC can guarantee enough phase margin.It is also proved that by decreasing the order of ESO,the phase margin can be increased.Furthermore,it is demonstrated that the phase margins of the ADRC-based systems can be almost uninfluenced by the uncertainties of the system parameters.Finally,the theoretical results are verified by both simulations and experiments on a motion control platform. 展开更多
关键词 Uncertain system Active disturbance rejection control(ADRC) Extended state observer(ESO) Phase margin Crossover frequency
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Insights into pollen-stigma recognition:self-incompatibility mechanisms serve as interspecies barriers in Brassicaceae?
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作者 Zijun Lan sheng zhong Li-Jia Qu 《aBIOTECH》 CSCD 2023年第2期176-179,共4页
A new study provides a comprehensive molecular mechanism that controls interspecific incompatibility of self-incompatible(SI)plants in the Brassicaceae.This finding points to a potentially promising path to break inte... A new study provides a comprehensive molecular mechanism that controls interspecific incompatibility of self-incompatible(SI)plants in the Brassicaceae.This finding points to a potentially promising path to break interspecific barriers and achieve introgression of desirable traits into crops from distant species among SI crops in the Brassicaceae. 展开更多
关键词 Pollen-stigma recognition SELF-INCOMPATIBILITY Interspecific barriers ROS
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FERONIA and reactive oxygen species:regulators in the self‑incompatibility responseand in interspecific pollination
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作者 Zihan Song sheng zhong Li‑Jia Qu 《Molecular Horticulture》 2023年第1期280-282,共3页
In plants,the stigma represents the initial site of pollenpistil communication and serves as a crucial location for rejection of incompatible pollen.In self-compatible(SC)plants,such as Arabidopsis thaliana from the B... In plants,the stigma represents the initial site of pollenpistil communication and serves as a crucial location for rejection of incompatible pollen.In self-compatible(SC)plants,such as Arabidopsis thaliana from the Brassicaceae family,pollen coat protein B(PCP-B)peptides were reported to compete with stigmatic RAPID ALKALINIZATION FACTOR 33(RALF33)peptides for binding to the FERONIA/ANJEA(FER/ANJ)receptors in the stigma to promote pollen hydration via inhibition of reactive oxygen species(ROS)production(Liu et al.2021). 展开更多
关键词 REACTIVE REGULATORS RAPID
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Cysteine-rich peptides: signals for pollen tube guidance, species isolation and beyond 被引量:4
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作者 sheng zhong Li-Jia Qu 《Science China(Life Sciences)》 SCIE CAS CSCD 2019年第9期1243-1245,共3页
In flowering plants,the two sperm cells are passive cargo transported into the ovule by pollen tubes (Zhang et al.,2017).Therefore,the precise guidance of pollen tubes is critical for successful double fertilization (... In flowering plants,the two sperm cells are passive cargo transported into the ovule by pollen tubes (Zhang et al.,2017).Therefore,the precise guidance of pollen tubes is critical for successful double fertilization (Dresselhaus and Franklin-Tong,2013).A series of male-female interactions are required to guarantee the spatiotemporal regulation of pollen tube guidance because during growth,pollen tubes continuously interact with different female tissues (Zhong et al.,2017;Zhong and Qu,2019).In the past two decades,there has been tremendous progress in elucidating the molecular mechanisms of pollen tube guidance regulation,mostly using Arabidopsis thaliana as a model system (Johnson et al.,2019). 展开更多
关键词 double FERTILIZATION POLLEN tube model system
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The signals to trigger the initiation of ovule enlargement are from the pollen tubes: The direct evidence 被引量:4
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作者 sheng zhong Jun Zhang Li-Jia Qu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2017年第9期600-603,共4页
In angiosperms, initiation of ovule enlargement represents the start of seed development, the molecular mechanism of which is not yet elucidated. It was previously reported that pollen tube contents, rather than doubl... In angiosperms, initiation of ovule enlargement represents the start of seed development, the molecular mechanism of which is not yet elucidated. It was previously reported that pollen tube contents, rather than double fertilization, can trigger ovule enlargement. However, it remains unclear whether the signal(s) to trigger the initiation of ovule enlargement are from the sperm cells or fromthe pollen tubes. Recently, we identified a mutant dropl- drop2-, which produces pollen tubes with no sperm cells. Taking advantage of this special genetic material, we conducted pollination assays, and found that the ovules pollinated with dropl- drop2- pollen could initiate the enlargement and exhibited significant enlarged sizes at 36h after pollination in comparison with those unpollinated ovules. However, the sizes of the ovules pollinated with drops- drop2- pollen are significantly smaller than those of the ovules pollinated with wildtype pollen. These results demonstrate that the pollen tube, rather than the sperm cells, release the signal to trigger the initiation of ovule enlargement, and that double fertilization is required for further enlargement of the seeds. 展开更多
关键词 The signals to trigger the initiation of ovule enlargement are from the pollen tubes The direct evidence Figure
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