BACKGROUND This study presents a case of rapidly developing respiratory failure due to antisynthetase syndrome(AS)following coronavirus disease 2019(COVID-19)in a 33-year-old man diagnosed with Klinefelter syndrome(KS...BACKGROUND This study presents a case of rapidly developing respiratory failure due to antisynthetase syndrome(AS)following coronavirus disease 2019(COVID-19)in a 33-year-old man diagnosed with Klinefelter syndrome(KS).CASE SUMMARY A 33-year-old man with a diagnosis of KS was admitted to the Department of Pulmonary and Critical Care Medicine of a tertiary hospital in China for fever and shortness of breath 2 wk after the onset of COVID-19.Computed tomography of both lungs revealed diffuse multiple patchy heightened shadows in both lungs,accompanied by signs of partial bronchial inflation.Metagenomic next-generation sequencing of the bronchoalveolar lavage fluid suggested absence of pathogen.A biopsy specimen revealed organizing pneumonia with alveolar septal thickening.Additionally,extensive auto-antibody tests showed strong positivity for anti-SSA,anti-SSB,anti-Jo-1,and anti-Ro-52.Following multidisciplinary discussions,the patient received a final diagnosis of AS,leading to rapidly progressing respiratory failure.CONCLUSION This study underscores the clinical progression of AS-associated interstitial lung disease subsequent to viral infections such as COVID-19 in patients diagnosed with KS.展开更多
The electrochemical dissolution and passivation of laser additive manufactured Ti6Al4V were investigated through Tafel polarization,potentiostatic polarization and AC impedance measurements.The results show that the s...The electrochemical dissolution and passivation of laser additive manufactured Ti6Al4V were investigated through Tafel polarization,potentiostatic polarization and AC impedance measurements.The results show that the solution treatment−aging(STA)process aggravates the element micro-segregation compared to the annealing process,leading to varied Al and V contents of the phases from different samples.It is proven that either Al-rich or V-rich condition can highly affect the electrochemical dissolution behaviors due to thermodynamical instability caused by element segregation.The dissolution rate in the metastable passivation process is controlled by the stability of the produced film that is affected by phases distribution,especially the difficult-to-dissolve phase.And then,the dissolution rate of the phases in the transpassivation region is consistent with the rank in the activation process because the dense film is not capable of being produced.Compared to the annealed sample,the higher dissolution rate of the STA sample is beneficial to the electrochemical machining(ECM)of Ti6Al4V.展开更多
Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in...Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in clinical practice reflects the principle of“using medicine as a soldier”.In the treatment of diabetes mellitus and its complications,Professor Lv made good use of mulberry leaves,mulberry branches,mulberry white bark,mulberry and mulberry parasitism,and gave full play to the modern pharmacological effects of mulberry drugs such as hypoglycemic,hypotensive and lipidemic in the combined use,and achieved satisfactory results.展开更多
As one of the key components of clinical trials, blinding, if successfully implemented, can help to mitigate the risks of implementation bias and measurement bias, consequently improving the validity and reliability o...As one of the key components of clinical trials, blinding, if successfully implemented, can help to mitigate the risks of implementation bias and measurement bias, consequently improving the validity and reliability of the trial results. However, successful blinding in clinical trials of traditional Chinese medicine(TCM) is hard to achieve, and the evaluation of blinding success through blinding assessment lacks established guidelines. Taking into account the challenges associated with blinding in the TCM field, here we present a framework for assessing blinding. Further, this study proposes a blinding assessment protocol for TCM clinical trials, building upon the framework and the existing methods. An assessment report checklist and an approach for evaluating the assessment results are presented based on the proposed protocol. It is anticipated that these improvements to blinding assessment will generate greater awareness among researchers, facilitate the standardization of blinding, and augment the blinding effectiveness. The use of this blinding assessment may further advance the quality and precision of TCM clinical trials and improve the accuracy of the trial results. The blinding assessment protocol will undergo continued optimization and refinement, drawing upon expert consensus and experience derived from clinical trials.展开更多
Background:Fibroblast-like synoviocytes(FLSs),resident mesenchymal cells of synovial joints,play an important role in the pathogenesis of rheumatoid arthritis(RA).Dickkopf-1(DKK-1)has been proposed to be a master regu...Background:Fibroblast-like synoviocytes(FLSs),resident mesenchymal cells of synovial joints,play an important role in the pathogenesis of rheumatoid arthritis(RA).Dickkopf-1(DKK-1)has been proposed to be a master regulator of bone remodeling in inflammatory arthritis.Here,potential impairation on the activity of FLSs derived from RA to small interfering RNAs(siRNAs)targeting DKK-1 was investigated.Methods:siRNAs targeting DKK-1 were transfected into FLSs of patients with RA.Interleukin(IL)-1β,IL-6,IL-8,matrix metalloproteinase(MMP)2,MMP3,MMP9,transforming growth factor(TGF)-pi,TGF-β2 and monocyte chemoattractant protein(MCP)-1 levels in the cell culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Invasion assay and 3H incorporation assay were utilized to investigate the effects of siRNAs targeting DKK-1 on FLSs invasion and cell proliferation,respectively.Western blotting was performed to analyze the expression of nuclear factor(NF)-kB,interleukin-1 receptor-associared kinase(IRAK)1,extracellular regulated protein kinases(ERK)1,Jun N-terminal kinase(JNK)and p-catenin in FLSs.Results:DKK-1 targeting siRNAs inhibited the expression of DKK-1 in FLSs(P<0.01).siRNAs induced a significant reduction of the levels of IL-6,IL-8,MMP2,MMP3 and MMP9 in FLSs compared to the control group(P<0.05).DKK-1 targeting siRNAs inhibited the proliferation and invasion of FLSs(P<0.05).Important molecules of pro-inflammatory signaling in FLSs,including IRAKI and ERK1,were decreased by the inhibition of DKK-1 in FLSs.In contrast,β-catenin,a pivotal downstream molecule of the Wnt signaling pathway was increased.Conclusions:By inhibiting DKK-1,we were able to inhibit the proliferation,invasion and pro-inflammatory cytokine secretion of FLSs derived from RA,which was mediated by the ERK or the IRAK-1 signaling pathway.These data indicate the application of DKK-1 silencing could be a potential therapeutic approach to RA.展开更多
基金Supported by the Natural Science Foundation of Jiangxi Province,No.20202BAB206002 and No.20224BAB216084.
文摘BACKGROUND This study presents a case of rapidly developing respiratory failure due to antisynthetase syndrome(AS)following coronavirus disease 2019(COVID-19)in a 33-year-old man diagnosed with Klinefelter syndrome(KS).CASE SUMMARY A 33-year-old man with a diagnosis of KS was admitted to the Department of Pulmonary and Critical Care Medicine of a tertiary hospital in China for fever and shortness of breath 2 wk after the onset of COVID-19.Computed tomography of both lungs revealed diffuse multiple patchy heightened shadows in both lungs,accompanied by signs of partial bronchial inflation.Metagenomic next-generation sequencing of the bronchoalveolar lavage fluid suggested absence of pathogen.A biopsy specimen revealed organizing pneumonia with alveolar septal thickening.Additionally,extensive auto-antibody tests showed strong positivity for anti-SSA,anti-SSB,anti-Jo-1,and anti-Ro-52.Following multidisciplinary discussions,the patient received a final diagnosis of AS,leading to rapidly progressing respiratory failure.CONCLUSION This study underscores the clinical progression of AS-associated interstitial lung disease subsequent to viral infections such as COVID-19 in patients diagnosed with KS.
基金The authors are grateful for the financial supports from the project of Ministry of Industry and Information Technology of China(No.2019-00899-1-1)the Natural Science Foundation of Shaanxi Province,China(No.2021JM-060)Fundamental Research Funds for the Central Universities,China(No.3102019QD0409).
文摘The electrochemical dissolution and passivation of laser additive manufactured Ti6Al4V were investigated through Tafel polarization,potentiostatic polarization and AC impedance measurements.The results show that the solution treatment−aging(STA)process aggravates the element micro-segregation compared to the annealing process,leading to varied Al and V contents of the phases from different samples.It is proven that either Al-rich or V-rich condition can highly affect the electrochemical dissolution behaviors due to thermodynamical instability caused by element segregation.The dissolution rate in the metastable passivation process is controlled by the stability of the produced film that is affected by phases distribution,especially the difficult-to-dissolve phase.And then,the dissolution rate of the phases in the transpassivation region is consistent with the rank in the activation process because the dense film is not capable of being produced.Compared to the annealed sample,the higher dissolution rate of the STA sample is beneficial to the electrochemical machining(ECM)of Ti6Al4V.
基金The Sixth Batch of National Academic Experience Inheritance Project for Senior Traditional Chinese Medicine Experts of State Administration of Traditional Chinese Medicine(No.JX332)National Traditional Chinese Medicine Experts Inheritance Studio Construction Project of State Administration of Traditional Chinese Medicine(No.JB063)。
文摘Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in clinical practice reflects the principle of“using medicine as a soldier”.In the treatment of diabetes mellitus and its complications,Professor Lv made good use of mulberry leaves,mulberry branches,mulberry white bark,mulberry and mulberry parasitism,and gave full play to the modern pharmacological effects of mulberry drugs such as hypoglycemic,hypotensive and lipidemic in the combined use,and achieved satisfactory results.
基金supported by the National Natural Science Foundation of China(No.82174530).
文摘As one of the key components of clinical trials, blinding, if successfully implemented, can help to mitigate the risks of implementation bias and measurement bias, consequently improving the validity and reliability of the trial results. However, successful blinding in clinical trials of traditional Chinese medicine(TCM) is hard to achieve, and the evaluation of blinding success through blinding assessment lacks established guidelines. Taking into account the challenges associated with blinding in the TCM field, here we present a framework for assessing blinding. Further, this study proposes a blinding assessment protocol for TCM clinical trials, building upon the framework and the existing methods. An assessment report checklist and an approach for evaluating the assessment results are presented based on the proposed protocol. It is anticipated that these improvements to blinding assessment will generate greater awareness among researchers, facilitate the standardization of blinding, and augment the blinding effectiveness. The use of this blinding assessment may further advance the quality and precision of TCM clinical trials and improve the accuracy of the trial results. The blinding assessment protocol will undergo continued optimization and refinement, drawing upon expert consensus and experience derived from clinical trials.
基金This work was supported by grants from the National Key R&D Program of China(No.2017YFA0105802)National Natural Science Foundation of China(Nos.81771678,81801617)Peking University People's Hospital Research and Development Funds(No.RDH 2017-02&RDX 2019-02).
文摘Background:Fibroblast-like synoviocytes(FLSs),resident mesenchymal cells of synovial joints,play an important role in the pathogenesis of rheumatoid arthritis(RA).Dickkopf-1(DKK-1)has been proposed to be a master regulator of bone remodeling in inflammatory arthritis.Here,potential impairation on the activity of FLSs derived from RA to small interfering RNAs(siRNAs)targeting DKK-1 was investigated.Methods:siRNAs targeting DKK-1 were transfected into FLSs of patients with RA.Interleukin(IL)-1β,IL-6,IL-8,matrix metalloproteinase(MMP)2,MMP3,MMP9,transforming growth factor(TGF)-pi,TGF-β2 and monocyte chemoattractant protein(MCP)-1 levels in the cell culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Invasion assay and 3H incorporation assay were utilized to investigate the effects of siRNAs targeting DKK-1 on FLSs invasion and cell proliferation,respectively.Western blotting was performed to analyze the expression of nuclear factor(NF)-kB,interleukin-1 receptor-associared kinase(IRAK)1,extracellular regulated protein kinases(ERK)1,Jun N-terminal kinase(JNK)and p-catenin in FLSs.Results:DKK-1 targeting siRNAs inhibited the expression of DKK-1 in FLSs(P<0.01).siRNAs induced a significant reduction of the levels of IL-6,IL-8,MMP2,MMP3 and MMP9 in FLSs compared to the control group(P<0.05).DKK-1 targeting siRNAs inhibited the proliferation and invasion of FLSs(P<0.05).Important molecules of pro-inflammatory signaling in FLSs,including IRAKI and ERK1,were decreased by the inhibition of DKK-1 in FLSs.In contrast,β-catenin,a pivotal downstream molecule of the Wnt signaling pathway was increased.Conclusions:By inhibiting DKK-1,we were able to inhibit the proliferation,invasion and pro-inflammatory cytokine secretion of FLSs derived from RA,which was mediated by the ERK or the IRAK-1 signaling pathway.These data indicate the application of DKK-1 silencing could be a potential therapeutic approach to RA.
