[Objectives]This study was conducted to screen different extraction methods for protein from potato tubers.[Methods]The bud eye,lateral and pith parts of Qingshu 168 and Kexin 1 were used as test materials,and were ex...[Objectives]This study was conducted to screen different extraction methods for protein from potato tubers.[Methods]The bud eye,lateral and pith parts of Qingshu 168 and Kexin 1 were used as test materials,and were extracted by acetone extraction,trichloroacetic acid(TCA)extraction,salt extraction,alcohol extraction and phenol extraction,respectively.The protein contents of different parts were determined by spectrophotometry.A 2-factor randomized block design was used to study the effects of varieties,extraction methods,sampling parts and combinations of different factors on protein content.[Results]The protein contents of different varieties were different,and Qingshu 168 was significantly higher than Kexin 1(P<0.05),reaching 128.0 mg/L.The protein contents obtained by different extraction methods were different.Acetone extraction,salt extraction and TCA extraction showed no significant differences in the protein content,but they were significantly higher than phenol extraction and alcohol extraction(P<0.05).The protein contents obtained by the former three were in the range of153.3-159.7 mg/L.The protein content in the bud eye part of potato tubers was significantly higher than those in the lateral and pith parts(P<0.05),reaching140.7 mg/L.There were significant differences among different combinations of varieties,extraction methods and sampling parts(P<0.05).The comprehensive comparison showed that the salt extraction method was suitable for the bud eye part of Qingshu 168,and the TCA extraction method was suitable for the bud eye part of Kexin No.1.[Conclusions]This study provides a reference for potato protein extraction and further research.展开更多
Nitrogen enrichment and land use are known to influence various ecosystems,but how these anthropogenic changes influence community and ecosystem responses to disturbance remains poorly understood.Here we investigated ...Nitrogen enrichment and land use are known to influence various ecosystems,but how these anthropogenic changes influence community and ecosystem responses to disturbance remains poorly understood.Here we investigated the effects of increased nitrogen input and mowing on the resistance and recovery of temperate semiarid grassland experiencing a three-year drought.Nitrogen addition increased grassland biomass recovery but decreased structural recovery after drought,whereas annual mowing increased grassland biomass recovery and structural recovery but reduced structural resistance to drought.The treatment effects on community biomass/structural resistance and recovery were largely modulated by the stability of the dominant species and asynchronous dynamics among species,and the community biomass resistance and recovery were also greatly driven by the stability of grasses.Community biomass resistance/recovery in response to drought was positively associated with its corresponding structural stability.Our study provides important experimental evidence that both nitrogen addition and mowing could substantially change grassland stability in both functional and structural aspects.Our findings emphasize the need to study changes across levels of ecological organization for a more complete understanding of ecosystem responses to disturbances under widespread environmental changes.展开更多
基金Supported by National Spark Program(2013GA880001)Innovation Project of College of Bioscience and Bioengineering,North Minzu University(2014S10)Hetian Region Science and Technology Bureau Project(202023)。
文摘[Objectives]This study was conducted to screen different extraction methods for protein from potato tubers.[Methods]The bud eye,lateral and pith parts of Qingshu 168 and Kexin 1 were used as test materials,and were extracted by acetone extraction,trichloroacetic acid(TCA)extraction,salt extraction,alcohol extraction and phenol extraction,respectively.The protein contents of different parts were determined by spectrophotometry.A 2-factor randomized block design was used to study the effects of varieties,extraction methods,sampling parts and combinations of different factors on protein content.[Results]The protein contents of different varieties were different,and Qingshu 168 was significantly higher than Kexin 1(P<0.05),reaching 128.0 mg/L.The protein contents obtained by different extraction methods were different.Acetone extraction,salt extraction and TCA extraction showed no significant differences in the protein content,but they were significantly higher than phenol extraction and alcohol extraction(P<0.05).The protein contents obtained by the former three were in the range of153.3-159.7 mg/L.The protein content in the bud eye part of potato tubers was significantly higher than those in the lateral and pith parts(P<0.05),reaching140.7 mg/L.There were significant differences among different combinations of varieties,extraction methods and sampling parts(P<0.05).The comprehensive comparison showed that the salt extraction method was suitable for the bud eye part of Qingshu 168,and the TCA extraction method was suitable for the bud eye part of Kexin No.1.[Conclusions]This study provides a reference for potato protein extraction and further research.
基金supported by the National Natural Science Foundation of China(32060284,31870441)the Natural Science Foundation of Inner Mongolia,China(2019JQ04)+2 种基金the Central Government Guides the Local Science and Technology Development Foundation(2020ZY0027)the National Science Foundation of the United States(DEB-1856318,CBET-1833988)the Natural Science Foundation of Hebei Province(C2022201042)。
文摘Nitrogen enrichment and land use are known to influence various ecosystems,but how these anthropogenic changes influence community and ecosystem responses to disturbance remains poorly understood.Here we investigated the effects of increased nitrogen input and mowing on the resistance and recovery of temperate semiarid grassland experiencing a three-year drought.Nitrogen addition increased grassland biomass recovery but decreased structural recovery after drought,whereas annual mowing increased grassland biomass recovery and structural recovery but reduced structural resistance to drought.The treatment effects on community biomass/structural resistance and recovery were largely modulated by the stability of the dominant species and asynchronous dynamics among species,and the community biomass resistance and recovery were also greatly driven by the stability of grasses.Community biomass resistance/recovery in response to drought was positively associated with its corresponding structural stability.Our study provides important experimental evidence that both nitrogen addition and mowing could substantially change grassland stability in both functional and structural aspects.Our findings emphasize the need to study changes across levels of ecological organization for a more complete understanding of ecosystem responses to disturbances under widespread environmental changes.
