Food allergy is a significant public health concern globally.Certain probiotics have been found to enhance food allergy by regulating immune-microbe interactions in animal models and patients.However,the effects of Bi...Food allergy is a significant public health concern globally.Certain probiotics have been found to enhance food allergy by regulating immune-microbe interactions in animal models and patients.However,the effects of Bifidobacterium lactis Probio-M8 on food allergy have not been thoroughly investigated.The present study examined the anti-allergic properties of Probio-M8,particularly in relation to immune response and gut microbiota composition.Results demonstrate that oral administration of Probio-M8 effectively mitigated the allergy symptoms triggered by ovalbumin(OVA)by ameliorating the morphological damage in the jejunum,reducing OVA-specific IgE and histamine levels in the serum,and suppressing Th2 cytokines(interleukin(IL)4 and IL-13)while increasing Th1 cytokines(interferon(IFN)γ)and regulatory T(Treg)cytokines(IL-10 and transforming growth factor(TGF)β1)in the culture supernatants of splenic cells.Furthermore,Probio-M8 effectively altered the diversity and composition of gut microbiota,particularly the relative abundances of Akkermansia_muciniphila in OVA-induced mice.Compared to the OVA group,the Probio-M8 group showed a decrease in the relative abundance of Akkermansia_muciniphila.In conclusion,Probio-M8 demonstrates the potential to alleviate food allergy by regulating the Th1/Th2 response and modulating gut microbiota,thereby offering a novel therapeutic strategy for patients with food allergy.展开更多
Roadways excavated in soft rocks at great depth are difficult to be maintained due to large deformation of surrounding rocks, which greatly influences the safety and efficiency of deep resources exploitation. During t...Roadways excavated in soft rocks at great depth are difficult to be maintained due to large deformation of surrounding rocks, which greatly influences the safety and efficiency of deep resources exploitation. During the excavation process of a deep soft rock tunnel, the rock wall may be compacted due to large deformation. In this paper, the technique to address this problem by a two-dimensional (2D) finite element software, large deformation engineering analyses software (LDEAS 1.0), is provided. By using the Lagrange multiplier method, the kinematic constraint of non-penetrating condition and static constraint of Coulomb friction are introduced to the governing equations in the form of incremental displacement. The numerical example demonstrates the efficiency of this technology. Deformations of a transportation tunnel in inclined soft rock strata at the depth of 1 000 m in Qishan coal mine and a tunnel excavated to three different depths are analyzed by two models, i.e. the additive decomposition model and polar decomposition model. It can be found that the deformation of the transportation tunnel is asymmetrical due to the inclination of rock strata. For extremely soft rock, large deformation can converge only for the additive decomposition model. The deformation of surrounding rocks increases with the increase in the tunnel depth for both models. At the same depth, the deformation calculated by the additive decomposition model is smaller than that by the polar decomposition model.展开更多
Objective： p53 is a tumor suppressor gene and is involved in the etiology of ovarian cancer. Studies investigating the associations between the p53 codon 72 polymorphism and ovarian cancer risk showed conflicting res...Objective： p53 is a tumor suppressor gene and is involved in the etiology of ovarian cancer. Studies investigating the associations between the p53 codon 72 polymorphism and ovarian cancer risk showed conflicting results. We performed this meta-analysis from eligible studies to evaluate this purported relationship. Methods： This meta-analysis was performed from 9 case-control studies, including 825 ovarian cases and 1073 controls. The fixed and random effect models were used to estimate the odds ratios（ORs） for various contrasts of this polymorphism. Results： The combined results based on all studies showed that a significantly decreased risk was associated with the variant Pro/Pro genotype, compared with Arg/Pro＋Arg/Arg genotypes（OR, 0.70; 95%CI, 0.51-0.95）. When stratifying the studies by ethnicity, we found that individuals with the variant genotype Pro/pro had a significantly decreased risk of ovarian cancer compared with Arg/Arg genotype（OR, 0.43; 95%CI, 0.20-0.89） and Arg/Pro＋Arg/Arg genotypes（OR, 0.61; 95%CI, 0.37-0.99） among Africans. Conclusion： This meta-analysis suggests that the p53 codon 72 polymorphism may contribute to genetic susceptibility to ovarian cancer. More studies based on larger sample size should be performed to confirm the findings.展开更多
基金the financial supporting by the National Key Research and Development Program of China(2022YFF1102400)National Natural Science Foundation of China(32102093)the Natural Science Foundation of Jiangsu Province(BK20210226)。
文摘Food allergy is a significant public health concern globally.Certain probiotics have been found to enhance food allergy by regulating immune-microbe interactions in animal models and patients.However,the effects of Bifidobacterium lactis Probio-M8 on food allergy have not been thoroughly investigated.The present study examined the anti-allergic properties of Probio-M8,particularly in relation to immune response and gut microbiota composition.Results demonstrate that oral administration of Probio-M8 effectively mitigated the allergy symptoms triggered by ovalbumin(OVA)by ameliorating the morphological damage in the jejunum,reducing OVA-specific IgE and histamine levels in the serum,and suppressing Th2 cytokines(interleukin(IL)4 and IL-13)while increasing Th1 cytokines(interferon(IFN)γ)and regulatory T(Treg)cytokines(IL-10 and transforming growth factor(TGF)β1)in the culture supernatants of splenic cells.Furthermore,Probio-M8 effectively altered the diversity and composition of gut microbiota,particularly the relative abundances of Akkermansia_muciniphila in OVA-induced mice.Compared to the OVA group,the Probio-M8 group showed a decrease in the relative abundance of Akkermansia_muciniphila.In conclusion,Probio-M8 demonstrates the potential to alleviate food allergy by regulating the Th1/Th2 response and modulating gut microbiota,thereby offering a novel therapeutic strategy for patients with food allergy.
基金Supported by the Fundamental Research Funds for the Central Universities of China (2009QL05)
文摘Roadways excavated in soft rocks at great depth are difficult to be maintained due to large deformation of surrounding rocks, which greatly influences the safety and efficiency of deep resources exploitation. During the excavation process of a deep soft rock tunnel, the rock wall may be compacted due to large deformation. In this paper, the technique to address this problem by a two-dimensional (2D) finite element software, large deformation engineering analyses software (LDEAS 1.0), is provided. By using the Lagrange multiplier method, the kinematic constraint of non-penetrating condition and static constraint of Coulomb friction are introduced to the governing equations in the form of incremental displacement. The numerical example demonstrates the efficiency of this technology. Deformations of a transportation tunnel in inclined soft rock strata at the depth of 1 000 m in Qishan coal mine and a tunnel excavated to three different depths are analyzed by two models, i.e. the additive decomposition model and polar decomposition model. It can be found that the deformation of the transportation tunnel is asymmetrical due to the inclination of rock strata. For extremely soft rock, large deformation can converge only for the additive decomposition model. The deformation of surrounding rocks increases with the increase in the tunnel depth for both models. At the same depth, the deformation calculated by the additive decomposition model is smaller than that by the polar decomposition model.
基金the National Natural Science Foun-dation of China(30571583 and 30271105)the Ph.D.Programs Foun-dation of Ministry of Education of China(20060312002)+3 种基金the NaturalScience Foundation of Jiangsu Province(BK2006231)the PostdoctoralScience Foundation of China(20060390293)the Postdoctoral ScienceFoundation of Jiangsu Province(0601049)"Qinglan Project"Fundation for the Young Academic Leader of Jiangsu Province(2006)
文摘Objective： p53 is a tumor suppressor gene and is involved in the etiology of ovarian cancer. Studies investigating the associations between the p53 codon 72 polymorphism and ovarian cancer risk showed conflicting results. We performed this meta-analysis from eligible studies to evaluate this purported relationship. Methods： This meta-analysis was performed from 9 case-control studies, including 825 ovarian cases and 1073 controls. The fixed and random effect models were used to estimate the odds ratios（ORs） for various contrasts of this polymorphism. Results： The combined results based on all studies showed that a significantly decreased risk was associated with the variant Pro/Pro genotype, compared with Arg/Pro＋Arg/Arg genotypes（OR, 0.70; 95%CI, 0.51-0.95）. When stratifying the studies by ethnicity, we found that individuals with the variant genotype Pro/pro had a significantly decreased risk of ovarian cancer compared with Arg/Arg genotype（OR, 0.43; 95%CI, 0.20-0.89） and Arg/Pro＋Arg/Arg genotypes（OR, 0.61; 95%CI, 0.37-0.99） among Africans. Conclusion： This meta-analysis suggests that the p53 codon 72 polymorphism may contribute to genetic susceptibility to ovarian cancer. More studies based on larger sample size should be performed to confirm the findings.
