In energy-dispersive X-ray fluorescence spectroscopy,the estimation of the pulse amplitude determines the accuracy of the spectrum measurement.The error generated by the amplitude estimation of the pulse output distor...In energy-dispersive X-ray fluorescence spectroscopy,the estimation of the pulse amplitude determines the accuracy of the spectrum measurement.The error generated by the amplitude estimation of the pulse output distorted by the measurement system leads to false peaks in the measured spectrum.To eliminate these false peaks and achieve an accurate estimation of the distorted pulse amplitude,a composite neural network model is proposed,which embeds long and short-term memory(LSTM)into the UNet structure.The UNet network realizes the fusion of pulse sequence features and the LSTM model realizes pulse amplitude estimation.The model is trained using simulated pulse datasets with different amplitudes and distortion times.For the pulse height estimation,the average relative error of the trained model on the test set was approximately 0.64%,which is 27.37% lower than that of the traditional trapezoidal shaping algorithm.Offline processing of a standard iron source further validated the pulse height estimation performance of the UNet-LSTM model.After estimating the amplitude of the distorted pulses using the model,the false peak area was reduced by approximately 91% over the full spectrum and was corrected to the characteristic peak region of interest(ROI).The corrected peak area accounted for approximately 1.32%of the characteristic peak ROI area.The results indicate that the model can accurately estimate the height of distorted pulses and has substantial corrective effects on false peaks.展开更多
为应对风力机齿轮箱振动信号压缩与重构过程存在复杂的参数设置问题,提出了基于模拟退火多种群遗传算法(Simulating Annealing and Multiple Population Genetic Algorithm,SA-MPGA)自适应设置过完备学习字典生成、振动信号压缩、压缩...为应对风力机齿轮箱振动信号压缩与重构过程存在复杂的参数设置问题,提出了基于模拟退火多种群遗传算法(Simulating Annealing and Multiple Population Genetic Algorithm,SA-MPGA)自适应设置过完备学习字典生成、振动信号压缩、压缩信号重构过程所需参数集。在传统遗传算法基础上引入多种群思想,增加了遗传算法对解空间的覆盖。在种群繁衍时个体选择引入模拟退火策略在种群进化过程中以不同概率接受一定程度的劣解,从而有助于遗传算法跳出局部最优解的缺陷。基于SA-MPGA的多参数自适应选择降低了传统遗传算法容易收敛到局部最优解的概率。应用实际工程数据验证基于SA-MPGA多参数优化问题,实验结果表明,在保持压缩率的前提下,基于模拟退火多种群算法比基于遗传算法重构信号与原始信号的峰值信噪比提升了16.5%,相关性提升了12.5%,均方根误差降低了13.4%。展开更多
Hard carbons are promising anode materials for sodium-ion batteries.To meet practical requirements,searching for durable and conductive carbon with a stable interface is of great importance.Here,we prepare a series of...Hard carbons are promising anode materials for sodium-ion batteries.To meet practical requirements,searching for durable and conductive carbon with a stable interface is of great importance.Here,we prepare a series of vanadiummodified hard carbon submicrospheres by using hydrothermal carbonization followed by high-temperature pyrolysis.Significantly,the introduction of vanadium can facilitate the nucleation and uniform growth of carbon spheres and generate abundant V-O-C interface bonds,thus optimizing the reaction kinetic.Meanwhile,the optimized hard carbon spheres modified by vanadium carbide,with sufficient pseudographitic domains,provide more active sites for Na ion migration and storage.As a result,the HC/VC-1300 electrode exhibits excellent Na storage performance,including a high capacity of 420 mAh g^(-1) at 50mA g^(-1) and good rate capability at 1 A g^(-1).This study proposes a new strategy for the synthesis of hard carbon spheres with high tap density and emphasizes the key role of pseudographitic structure for Na storage and interface stabilization.展开更多
Water splitting is important to the conversion and storage of renewable energy,but slow kinetics of the oxygen evolution reaction(OER)greatly limits its utility.Here,under visible light illumination,the p-n WO_(3)/SnS...Water splitting is important to the conversion and storage of renewable energy,but slow kinetics of the oxygen evolution reaction(OER)greatly limits its utility.Here,under visible light illumination,the p-n WO_(3)/SnSe_(2)(WS)heterojunction significantly activates OER catalysis of CoFe-layered double hydroxide(CF)/carbon nanotubes(CNTs).Specifically,the catalyst achieves an overpotential of 224 mV at 10 mA cm^(-2)and a small Tafel slope of 47 mV dec^(-1),superior to RuO_(2)and most previously reported transition metal-based OER catalysts.The p-n WS heterojunction shows strong light absorption to produce photogenerated carriers.The photogenerated holes are trapped by CF to suppresses the charge recombination and facilitate charge transfer,which accelerates OER kinetics and boost the activity for the OER.This work highlights the possibility of using heterojunctions to activate OER catalysis and advances the design of energy-efficient catalysts for water oxidation systems using solar energy.展开更多
BACKGROUND In the past 3 years,the global pandemic of coronavirus disease 2019(COVID-19)has posed a great threat to human life and safety.Among the causes of death in COVID-19 patients,combined or secondary bacterial ...BACKGROUND In the past 3 years,the global pandemic of coronavirus disease 2019(COVID-19)has posed a great threat to human life and safety.Among the causes of death in COVID-19 patients,combined or secondary bacterial infection is an important factor.As a special group,pregnant women experience varying degrees of change in their immune status,cardiopulmonary function,and anatomical structure during pregnancy,which puts them at higher risk of contracting COVID-19.COVID-19 infection during pregnancy is associated with increased adverse events such as hospitalisation,admission to the intensive care unit,and mechanical ventilation.Therefore,pregnancy combined with coinfection of COVID-19 and bacteria often leads to critical respiratory failure,posing severe challenges in the diagnosis and treatment process.CASE SUMMARY We report a case of COVID-19 complicated with Staphylococcus aureus(S.aureus)coinfection in a pre-gnant woman at 34 wk of gestation.Her rapid progression of pulmonary lesions caused severe respiratory failure,and she received noninvasive ventilator-assisted respiratory treatment.Subsequently,we delivered a foetus via emergency caesarean section after accelerating the maturity of the foetal pulmonary system,and the respiratory condition of the puerperant woman significantly improved after the delivery of the foetus.Lavage fluid was taken under tracheoscopy to quickly search for pathogens by the metagenomic nextgeneration sequencing(mNGS),and both COVID-19 and S.aureus were detected.After targeted anti-infective treatment,the maternal condition gradually improved,and the patient was discharged from the hospital.CONCLUSION The coinfection of pregnancy with COVID-19 and bacteria often leads to critical respiratory failure,which is a great challenge in the process of diagnosis and treatment.It is crucial to choose the right time to deliver the foetus and to quickly find pathogens by mNGS.展开更多
Using the RFM(Recency,Frequency,Monetary value)model can provide valuable insights about customer clusterswhich is the core of customer relationship management.Due to accurate customer segment coming from dynamic weig...Using the RFM(Recency,Frequency,Monetary value)model can provide valuable insights about customer clusterswhich is the core of customer relationship management.Due to accurate customer segment coming from dynamic weighted applications,in-depth targeted marketing may also use type of dynamic weight of R,F and M as factors.In this paper,we present our dynamic weighted RFM approach which is intended to improve the performance of customer segmentation by using the factors and variations to attain dynamic weights.Our dynamic weight approach is a kind of Custom method in essential which roots in the understanding of the data set.Firstly,Analytic Hierarchy Process is used to calculate the subjective weight,then the entropy method is applied to calculate the objective weight.Finally,we use comprehensive integration weighting method to combine the subjective and objective weight to obtain the final weight of the index to calculate the individual user value and quantify the user value difference.The experiment shows that the dynamic weight we used in RFM model is vital,affects the customer segmentation performance positively.Also,this study indicates that customer segments containing dynamic weighted RFM scores bring about stronger and more accurate association rules for the understanding of customer behavior.At last,we discuss the limitations of RFM analysis.展开更多
Objective:To investigate the distribution of pathogens and drug resistance in bile and the association between the pregane X receptor(PXR) gene polymorphisms,traditional Chinese medicine(TCM) syndromes and the risk of...Objective:To investigate the distribution of pathogens and drug resistance in bile and the association between the pregane X receptor(PXR) gene polymorphisms,traditional Chinese medicine(TCM) syndromes and the risk of cholesterol gallstone disease(CGD).Methods:A total of 392 samples were enrolled in this study from January 2014 to February 2015.among which 192 patients were with CGD.and 200 samples were healthy.Strains were isolated and susceptibility testing was the disk diffusion method susceptibility testing.The patients were divided into hepatochlic hygropyrexia.stagnation of liver-qi.and the accumulation of damp.The PXR gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism.The association between the PXR gene polymorphisms and the risk of CGD was examined by logistic regression analysis.Results:A total of 192 cases were detected in 230 of bile culture pathogens,including Grain-negative bacteria 133(57.83%),Gram-positive bacteria76(33.04%),and fungi 21(9.13%).The top five pathogens were Escherichia coli,Klebsiella pneumoniae.Enterococcus faecalis,Candida albicans,and Enterococcus feces,ot which 110 cases was of single infection.48 cases of mixed infection of two strains,eight cases of mixed infection of three bacteria.Among 59 Escherichia coli,the yield extended-spectrum beta-laetamases had 40(67.80%).The hepatochlic hygropyrexia was the most TCM syndrome,followed by stagnation of liver-qi.and the accumulation of damp was least.Different pathogens and the rs6785049 genotypes distributed differently in cholelithiasis patients with different TCM syndromes(P<0.05).In hepatochlic hygropyrexia patients the Gram-negative bacteria was most.There was significant differences between CGD group and control group in rs6785049(P<0.001).Comparison with wild-type portable GG.GA genotype increased the risk of the occurrence of gallstones(OR=0.40.95%CI:0.16-0.79);likewise,carrying the GA + AA genotype also increased the risk(OR=0.38,95%CI:0.19-0.81).There was no significant differences in rs2276707,rs3814055 site polymorphic loci alleles in CGD group and control group.Conclusions:In the treatment of cholelithiasis,bile samples should be collected for bacterial culture and sensitivity test,and drugs should be strictly chosen based on the results.The rs6785049 polymorphisms in PXR gene may increase the risk of gallstones ontogeny,and gallstones can he early detected and prevented by detecting genotypes.rs6785049 polymorphisms in PXR gene may has relationship with TCM syndromes.展开更多
Objective: To study the effect of emodin on protein and gene expressions of the massagers in mobility signal transduction system of cholecyst smooth muscle cells in guinea pig with cholesterol calculus. Methods: The g...Objective: To study the effect of emodin on protein and gene expressions of the massagers in mobility signal transduction system of cholecyst smooth muscle cells in guinea pig with cholesterol calculus. Methods: The guinea pigs were randomly divided into 4 groups, such as control group, gall-stone(GS) group, emodin group and ursodesoxycholic acid(UA) group. Cholesterol calculus models were induced in guinea pigs of GS, emodin and UA groups of induced models by lithogenic diet, while emodin or UA were given to the corresponding group for 7 weeks. The histomorphological and ultrastructure change of gallbladder were detected by microscope and electron microscope, the content of plasma cholecystokinin(CCK) and [Ca^(2+)]i were analyzed successively by radioimmunoassay and flow cytometry. The protein and mR NA of Gsα, Giα and Cap in cholecyst cells were determined by western blotting and real time polymerase chain reaction(RT-PCR). Results: Emodin or UA can relieve pathogenic changes in epithelial cells and muscle cells in gallbladder of guinea pig with cholesterol calculus by microscope and transmission electron microscope. In the cholecyst cells of GS group, CCK levels in plasma and [Ca^(2+)]i decreased, the protein and m RNA of GS group were downregulated,the protein and m RNA of Gi and Cap were up-regulated. Emodin significantly decreased the formative rate of gallstone, improved the pathogenic change in epithelial cells and muscle cells, increased CCK levels in plasma and [Ca^(2+)]i in cholecyst cells, enhanced the protein and mR NA of Gs in cholecyst cells, reduced the protein and mR NA of Gi and Cap in cholecyst cells in guinea pig with cholesterol calculus. Conclusion: The dysfunction of gallbladder contraction gives rise to the disorders of mobility signal transduction system in cholecyst smooth muscle cells, including low content of plasma CCK and [Ca^(2+)]i in cholecyst cells, abnormal protein and mRNA of Gs, Gi and Cap. Emodin can enhance the contractibility of gallbladder and alleviate cholestasis by regulating plasma CCK levels, [Ca2+]i in cholecyst cells and the protein and mR NA of Gs, Gi and Cap.展开更多
Constructing a step-scheme heterojunction at the interface between two semiconductors is an efficient way to optimize the redox ability and accelerate the charge carrier separation of a photocatalytic system for achie...Constructing a step-scheme heterojunction at the interface between two semiconductors is an efficient way to optimize the redox ability and accelerate the charge carrier separation of a photocatalytic system for achieving high photocatalytic performance.In this study,we prepared a hierarchical ZnO@ZnS step-scheme photocatalyst by incorporating ZnS into the outer shell of hollow ZnO microspheres via a simple in situ sulfidation strategy.The ZnO@ZnS step-scheme photocatalysts had a large surface area,high light utilization capacity,and superior separation efficiency for photogenerated charge carriers.In addition,the material simulation revealed that the formation of the step-scheme heterojunction between ZnO and ZnS was due to the presence of the built-in electric field.Our study paves the way for design of high-performance photocatalysts for H_(2) production.展开更多
Disulfide bond-bridging strategy has been extensively utilized to construct tumor specificity-responsive aliphatic prodrug nanoparticles(PNPs) for precise cancer therapy. Yet, there is no research shedding light on th...Disulfide bond-bridging strategy has been extensively utilized to construct tumor specificity-responsive aliphatic prodrug nanoparticles(PNPs) for precise cancer therapy. Yet, there is no research shedding light on the impacts of the saturation and cis-trans configuration of aliphatic tails on the self-assembly capacity of disulfide bond-linked prodrugs and the in vivo delivery fate of PNPs. Herein, five disulfide bond-linked docetaxelfatty acid prodrugs are designed and synthesized by using stearic acid, elaidic acid, oleic acid, linoleic acid and linolenic acid as the aliphatic tails, respectively. Interestingly, the cistrans configuration of aliphatic tails significantly influences the self-assembly features of prodrugs, and elaidic acid-linked prodrug with a trans double bond show poor self-assembly capacity. Although the aliphatic tails have almost no effect on the redox-sensitive drug release and cytotoxicity, different aliphatic tails significantly influence the chemical stability of prodrugs and the colloidal stability of PNPs, thus affecting the in vivo pharmacokinetics, biodistribution and antitumor efficacy of PNPs. Our findings illustrate how aliphatic tails affect the assembly characteristic of disulfide bond-linked aliphatic prodrugs and the in vivo delivery fate of PNPs, and thus provide theoretical basis for future development of disulfide bond-bridged aliphatic prodrugs.展开更多
Aqueous zinc metal batteries are noted for their costeffectiveness,safety and environmental friendliness.However,the water-induced notorious issues such as continuous electrolyte decomposition and uneven Zn electroche...Aqueous zinc metal batteries are noted for their costeffectiveness,safety and environmental friendliness.However,the water-induced notorious issues such as continuous electrolyte decomposition and uneven Zn electrochemical deposition remarkably restrict the development of the long-life zinc metal batteries.In this study,zwitterionic sulfobetaine is introduced to copolymerize with acrylamide in zinc perchlorate(Zn(ClO;);)solution.The designed gel framework with hydrophilic and charged groups can firmly anchor water molecules and construct ion migration channels to accelerate ion transport.The in situ generated hybrid interface,which is composed of the organic functionalized outer layer and inorganic Clcontaining inner layer,can synergically lower the mass transfer overpotential,reduce water-related side reactions and lead to uniform Zn deposition.Such a novel electrolyte configuration enables Zn//Zn cells with an ultra-long cycling life of over 3000 h and a low polarization potential(~0.03 V)and Zn//Cu cells with high Coulombic efficiency of 99.18%for 1000 cycles.Full cells matched with MnO;cathodes delivered laudable cycling stability and impressive shelving ability.Besides,the flexible quasi-solid-state batteries which are equipped with the anti-vandalism ability(such as cutting,hammering and soaking)can successfully power the LED simultaneously.Such a safe,processable and durable hydrogel promises significant application potential for long-life flexible electronic devices.展开更多
Objective:To study the mechanism of insulin resistance in the cholesterol gallstone formation from insulin signal transduction pathway so as to reveal the possible mechanism and the effective role of Albiflorin Granul...Objective:To study the mechanism of insulin resistance in the cholesterol gallstone formation from insulin signal transduction pathway so as to reveal the possible mechanism and the effective role of Albiflorin Granule on preventing the cholesterol gallstones.Methods:Serum triglycerides(TG),free fatty acid(FFA),and total cholesterol(TC) from different groups were measured and liver cells Ins R,PKB,IKK-β protein expression levels were detected by western blotting.Results:Albiflorin significantly decreased the cholesterol gallstone formation rate,increased glucose infusion rate in gallstone guinea pigs and improved insulin resistance.Compared with the normal group,insulin receptor and PKB protein expression in GS group were significantly reduced.IKK-β protein in the GS group increased significantly and Albiflorin could reduce IKK-β protein expression in guinea pig liver cells.Conclusions:The model of insulin resistance in cholesterol gallstone guinea pig was successfully established,which plays an important role in the cholesterol gallstone formation.All aspects of insulin signaling pathway are involved in gallstone formation.Albiflorin can regulate various aspects of insulin signal transduction pathway to prevent the formation of gallbladder.展开更多
Mitochondria are currently known as novel targets for treating cancer,especially for tumors displaying multidrug resistance(MDR). This present study aimed to develop a mitochondriatargeted delivery system by using tri...Mitochondria are currently known as novel targets for treating cancer,especially for tumors displaying multidrug resistance(MDR). This present study aimed to develop a mitochondriatargeted delivery system by using triphenylphosphonium cation(TPP+)-conjugated Brij 98 as the functional stabilizer to modify paclitaxel(PTX) nanocrystals(NCs) against drugresistant cancer cells. Evaluations were performed on 2 D monolayer and 3 D multicellular spheroids(MCs) of MCF-7 cells and MCF-7/ADR cells. In comparison with free PTX and the non-targeted PTX NCs,the targeted PTX NCs showed the strongest cytotoxicity against both2 D MCF-7 and MCF-7/ADR cells,which was correlated with decreased mitochondrial membrane potential. The targeted PTX NCs exhibited deeper penetration on MCF-7 MCs and more significant growth inhibition on both MCF-7 and MCF-7/ADR MCs. The proposed strategy indicated that the TPP+-modified NCs represent a potentially viable approach for targeted chemotherapeutic molecules to mitochondria. This strategy might provide promising therapeutic outcomes to overcome MDR.展开更多
One of the major barriers in utilizing prodrug nanocarriers for cancer therapy is the slow release of parent drug in tumors.Tumor cells generally display the higher oxidative level than normal cells,and also displayed...One of the major barriers in utilizing prodrug nanocarriers for cancer therapy is the slow release of parent drug in tumors.Tumor cells generally display the higher oxidative level than normal cells,and also displayed the heterogeneity in terms of redox homeostasis level.We previously found that the disulfide bond-linkage demonstrates surprising oxidationsensitivity to form the hydrophilic sulfoxide and sulphone groups.Herein,we develop oxidation-strengthened prodrug nanosystem loaded with pyropheophorbide a(PPa)to achieve light-activatable cascade drug release and enhance therapeutic efficacy.The disulfide bond-driven prodrug nanosystems not only respond to the redox-heterogeneity in tumor,but also respond to the exogenous oxidant(singlet oxygen)elicited by photosensitizers.Once the prodrug nanoparticles(NPs)are activated under irradiation,they would undergo an oxidative self-strengthened process,resulting in a facilitated drug cascade release.The IC50 value of the PPa@PTX-S-S NPs without irradiation was 2-fold higher than those of NPs plus irradiation.In vivo,the PPa@PTX prodrug NPs display prolonged systemic circulation and increased accumulation in tumor site.The PPa@PTXS-S NPs showed much higher efficiency than free PTX or the PPa@PTX-C-C NPs to suppress the growth of 4 T1 tumors.Therefore,this novel oxidation-strengthened disulfide-bridged prodrug-nanosystem has a great potential in the enhanced efficacy of cancer synergetic photochemotherapy.展开更多
Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processi...Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.展开更多
Photocatalytic hydrogen(H_(2))production via water splitting in the absence of sacrificial agents is a promising strategy for producing clean and sustainable hydrogen energy from solar energy.However,the realization o...Photocatalytic hydrogen(H_(2))production via water splitting in the absence of sacrificial agents is a promising strategy for producing clean and sustainable hydrogen energy from solar energy.However,the realization of a photocatalytic pure water splitting system with desirable efficiency is still a huge challenge.Herein,visible light photocatalytic H_(2) production from pure water splitting was successfully achieved using a g-C_(3)N_(4)/CoTiO_(3) S-scheme heterojunction photocatalyst in the absence of sacrificial agents.An optimum hydrogen evolution rate of 118μmol∙h^(−1)∙g^(−1) was reached with the addition of 1.5 wt%CoTiO_(3).The remarkably promoted hydrogen evolution rate was attributed to the intensified light absorption coupled with the synergistic effect of visible light responsive CoTiO_(3),the promoted efficiency in charge separation,and the reserved strong redox capacity induced by the S-scheme charge transfer mechanism.This work provides an alternative to visible light-responding oxidation photocatalysts for the construction of S-scheme heterojunctions and high-efficiency photocatalytic systems for pure water splitting.展开更多
基金supported by the Open Project of Guangxi Key Laboratory of Nuclear Physics and Nuclear Technology(No.NLK2022-05)the Central Government Guidance Funds for Local Scientific and Technological Development,China(No.Guike ZY22096024)+5 种基金the Sichuan Natural Science Youth Fund Project(No.2023NSFSC1366)Key R&D Projects of Sichuan Provincial Department of Science and Technology(No.2023YFG0287)the Open Research Fund of National Engineering Research Center for Agro-Ecological Big Data Analysis&Application,Anhui University(No.AE202209)the National Natural Science Youth Foundation of China(No.12305214)the Vanadium and Titanium Resource Comprehensive Utilization Key Laboratory of Sichuan Province(No.2023FTSZ03)the Key Laboratory of Interior Layout optimization and Security,Institutions of Higher Education of Sichuan Province(No.2023SNKJ-01)。
文摘In energy-dispersive X-ray fluorescence spectroscopy,the estimation of the pulse amplitude determines the accuracy of the spectrum measurement.The error generated by the amplitude estimation of the pulse output distorted by the measurement system leads to false peaks in the measured spectrum.To eliminate these false peaks and achieve an accurate estimation of the distorted pulse amplitude,a composite neural network model is proposed,which embeds long and short-term memory(LSTM)into the UNet structure.The UNet network realizes the fusion of pulse sequence features and the LSTM model realizes pulse amplitude estimation.The model is trained using simulated pulse datasets with different amplitudes and distortion times.For the pulse height estimation,the average relative error of the trained model on the test set was approximately 0.64%,which is 27.37% lower than that of the traditional trapezoidal shaping algorithm.Offline processing of a standard iron source further validated the pulse height estimation performance of the UNet-LSTM model.After estimating the amplitude of the distorted pulses using the model,the false peak area was reduced by approximately 91% over the full spectrum and was corrected to the characteristic peak region of interest(ROI).The corrected peak area accounted for approximately 1.32%of the characteristic peak ROI area.The results indicate that the model can accurately estimate the height of distorted pulses and has substantial corrective effects on false peaks.
文摘为应对风力机齿轮箱振动信号压缩与重构过程存在复杂的参数设置问题,提出了基于模拟退火多种群遗传算法(Simulating Annealing and Multiple Population Genetic Algorithm,SA-MPGA)自适应设置过完备学习字典生成、振动信号压缩、压缩信号重构过程所需参数集。在传统遗传算法基础上引入多种群思想,增加了遗传算法对解空间的覆盖。在种群繁衍时个体选择引入模拟退火策略在种群进化过程中以不同概率接受一定程度的劣解,从而有助于遗传算法跳出局部最优解的缺陷。基于SA-MPGA的多参数自适应选择降低了传统遗传算法容易收敛到局部最优解的概率。应用实际工程数据验证基于SA-MPGA多参数优化问题,实验结果表明,在保持压缩率的前提下,基于模拟退火多种群算法比基于遗传算法重构信号与原始信号的峰值信噪比提升了16.5%,相关性提升了12.5%,均方根误差降低了13.4%。
基金National Natural Science Foundation of China,Grant/Award Numbers:51874362,51932011,52002407Scientific Research Project of Hunan Provincial Department of Education,Grant/Award Number:21B0815。
文摘Hard carbons are promising anode materials for sodium-ion batteries.To meet practical requirements,searching for durable and conductive carbon with a stable interface is of great importance.Here,we prepare a series of vanadiummodified hard carbon submicrospheres by using hydrothermal carbonization followed by high-temperature pyrolysis.Significantly,the introduction of vanadium can facilitate the nucleation and uniform growth of carbon spheres and generate abundant V-O-C interface bonds,thus optimizing the reaction kinetic.Meanwhile,the optimized hard carbon spheres modified by vanadium carbide,with sufficient pseudographitic domains,provide more active sites for Na ion migration and storage.As a result,the HC/VC-1300 electrode exhibits excellent Na storage performance,including a high capacity of 420 mAh g^(-1) at 50mA g^(-1) and good rate capability at 1 A g^(-1).This study proposes a new strategy for the synthesis of hard carbon spheres with high tap density and emphasizes the key role of pseudographitic structure for Na storage and interface stabilization.
基金the National Natural Science Foundation of China(No.41807213)the Hydrogeo-logical Survey Project of Huangshui River(No.DD20190331).
文摘Water splitting is important to the conversion and storage of renewable energy,but slow kinetics of the oxygen evolution reaction(OER)greatly limits its utility.Here,under visible light illumination,the p-n WO_(3)/SnSe_(2)(WS)heterojunction significantly activates OER catalysis of CoFe-layered double hydroxide(CF)/carbon nanotubes(CNTs).Specifically,the catalyst achieves an overpotential of 224 mV at 10 mA cm^(-2)and a small Tafel slope of 47 mV dec^(-1),superior to RuO_(2)and most previously reported transition metal-based OER catalysts.The p-n WS heterojunction shows strong light absorption to produce photogenerated carriers.The photogenerated holes are trapped by CF to suppresses the charge recombination and facilitate charge transfer,which accelerates OER kinetics and boost the activity for the OER.This work highlights the possibility of using heterojunctions to activate OER catalysis and advances the design of energy-efficient catalysts for water oxidation systems using solar energy.
文摘BACKGROUND In the past 3 years,the global pandemic of coronavirus disease 2019(COVID-19)has posed a great threat to human life and safety.Among the causes of death in COVID-19 patients,combined or secondary bacterial infection is an important factor.As a special group,pregnant women experience varying degrees of change in their immune status,cardiopulmonary function,and anatomical structure during pregnancy,which puts them at higher risk of contracting COVID-19.COVID-19 infection during pregnancy is associated with increased adverse events such as hospitalisation,admission to the intensive care unit,and mechanical ventilation.Therefore,pregnancy combined with coinfection of COVID-19 and bacteria often leads to critical respiratory failure,posing severe challenges in the diagnosis and treatment process.CASE SUMMARY We report a case of COVID-19 complicated with Staphylococcus aureus(S.aureus)coinfection in a pre-gnant woman at 34 wk of gestation.Her rapid progression of pulmonary lesions caused severe respiratory failure,and she received noninvasive ventilator-assisted respiratory treatment.Subsequently,we delivered a foetus via emergency caesarean section after accelerating the maturity of the foetal pulmonary system,and the respiratory condition of the puerperant woman significantly improved after the delivery of the foetus.Lavage fluid was taken under tracheoscopy to quickly search for pathogens by the metagenomic nextgeneration sequencing(mNGS),and both COVID-19 and S.aureus were detected.After targeted anti-infective treatment,the maternal condition gradually improved,and the patient was discharged from the hospital.CONCLUSION The coinfection of pregnancy with COVID-19 and bacteria often leads to critical respiratory failure,which is a great challenge in the process of diagnosis and treatment.It is crucial to choose the right time to deliver the foetus and to quickly find pathogens by mNGS.
基金the National Natural Science Foundation of China(No.72073041)Open Foundation for the University Innovation Platform in Hunan Province(No.18K103)+2 种基金2011 Collaborative Innovation Center for Development and Utilization of Finance and Economics Big Data Property,Universities of Hunan Province,Open Project(Nos.20181901CRP03,20181901CRP04,20181901CRP05)2020 Hunan Provincial Higher Education Teaching Reform Research Project(Nos.HNJG-2020-1130,HNJG-2020-1124)2020 General Project of Hunan Social Science Fund(No.20B16).
文摘Using the RFM(Recency,Frequency,Monetary value)model can provide valuable insights about customer clusterswhich is the core of customer relationship management.Due to accurate customer segment coming from dynamic weighted applications,in-depth targeted marketing may also use type of dynamic weight of R,F and M as factors.In this paper,we present our dynamic weighted RFM approach which is intended to improve the performance of customer segmentation by using the factors and variations to attain dynamic weights.Our dynamic weight approach is a kind of Custom method in essential which roots in the understanding of the data set.Firstly,Analytic Hierarchy Process is used to calculate the subjective weight,then the entropy method is applied to calculate the objective weight.Finally,we use comprehensive integration weighting method to combine the subjective and objective weight to obtain the final weight of the index to calculate the individual user value and quantify the user value difference.The experiment shows that the dynamic weight we used in RFM model is vital,affects the customer segmentation performance positively.Also,this study indicates that customer segments containing dynamic weighted RFM scores bring about stronger and more accurate association rules for the understanding of customer behavior.At last,we discuss the limitations of RFM analysis.
基金supported by the National Science Foundation of China(30672698). "The first two authors contributed equally to this work
文摘Objective:To investigate the distribution of pathogens and drug resistance in bile and the association between the pregane X receptor(PXR) gene polymorphisms,traditional Chinese medicine(TCM) syndromes and the risk of cholesterol gallstone disease(CGD).Methods:A total of 392 samples were enrolled in this study from January 2014 to February 2015.among which 192 patients were with CGD.and 200 samples were healthy.Strains were isolated and susceptibility testing was the disk diffusion method susceptibility testing.The patients were divided into hepatochlic hygropyrexia.stagnation of liver-qi.and the accumulation of damp.The PXR gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism.The association between the PXR gene polymorphisms and the risk of CGD was examined by logistic regression analysis.Results:A total of 192 cases were detected in 230 of bile culture pathogens,including Grain-negative bacteria 133(57.83%),Gram-positive bacteria76(33.04%),and fungi 21(9.13%).The top five pathogens were Escherichia coli,Klebsiella pneumoniae.Enterococcus faecalis,Candida albicans,and Enterococcus feces,ot which 110 cases was of single infection.48 cases of mixed infection of two strains,eight cases of mixed infection of three bacteria.Among 59 Escherichia coli,the yield extended-spectrum beta-laetamases had 40(67.80%).The hepatochlic hygropyrexia was the most TCM syndrome,followed by stagnation of liver-qi.and the accumulation of damp was least.Different pathogens and the rs6785049 genotypes distributed differently in cholelithiasis patients with different TCM syndromes(P<0.05).In hepatochlic hygropyrexia patients the Gram-negative bacteria was most.There was significant differences between CGD group and control group in rs6785049(P<0.001).Comparison with wild-type portable GG.GA genotype increased the risk of the occurrence of gallstones(OR=0.40.95%CI:0.16-0.79);likewise,carrying the GA + AA genotype also increased the risk(OR=0.38,95%CI:0.19-0.81).There was no significant differences in rs2276707,rs3814055 site polymorphic loci alleles in CGD group and control group.Conclusions:In the treatment of cholelithiasis,bile samples should be collected for bacterial culture and sensitivity test,and drugs should be strictly chosen based on the results.The rs6785049 polymorphisms in PXR gene may increase the risk of gallstones ontogeny,and gallstones can he early detected and prevented by detecting genotypes.rs6785049 polymorphisms in PXR gene may has relationship with TCM syndromes.
基金supported by the National Science Foundation of China(30672698)
文摘Objective: To study the effect of emodin on protein and gene expressions of the massagers in mobility signal transduction system of cholecyst smooth muscle cells in guinea pig with cholesterol calculus. Methods: The guinea pigs were randomly divided into 4 groups, such as control group, gall-stone(GS) group, emodin group and ursodesoxycholic acid(UA) group. Cholesterol calculus models were induced in guinea pigs of GS, emodin and UA groups of induced models by lithogenic diet, while emodin or UA were given to the corresponding group for 7 weeks. The histomorphological and ultrastructure change of gallbladder were detected by microscope and electron microscope, the content of plasma cholecystokinin(CCK) and [Ca^(2+)]i were analyzed successively by radioimmunoassay and flow cytometry. The protein and mR NA of Gsα, Giα and Cap in cholecyst cells were determined by western blotting and real time polymerase chain reaction(RT-PCR). Results: Emodin or UA can relieve pathogenic changes in epithelial cells and muscle cells in gallbladder of guinea pig with cholesterol calculus by microscope and transmission electron microscope. In the cholecyst cells of GS group, CCK levels in plasma and [Ca^(2+)]i decreased, the protein and m RNA of GS group were downregulated,the protein and m RNA of Gi and Cap were up-regulated. Emodin significantly decreased the formative rate of gallstone, improved the pathogenic change in epithelial cells and muscle cells, increased CCK levels in plasma and [Ca^(2+)]i in cholecyst cells, enhanced the protein and mR NA of Gs in cholecyst cells, reduced the protein and mR NA of Gi and Cap in cholecyst cells in guinea pig with cholesterol calculus. Conclusion: The dysfunction of gallbladder contraction gives rise to the disorders of mobility signal transduction system in cholecyst smooth muscle cells, including low content of plasma CCK and [Ca^(2+)]i in cholecyst cells, abnormal protein and mRNA of Gs, Gi and Cap. Emodin can enhance the contractibility of gallbladder and alleviate cholestasis by regulating plasma CCK levels, [Ca2+]i in cholecyst cells and the protein and mR NA of Gs, Gi and Cap.
文摘Constructing a step-scheme heterojunction at the interface between two semiconductors is an efficient way to optimize the redox ability and accelerate the charge carrier separation of a photocatalytic system for achieving high photocatalytic performance.In this study,we prepared a hierarchical ZnO@ZnS step-scheme photocatalyst by incorporating ZnS into the outer shell of hollow ZnO microspheres via a simple in situ sulfidation strategy.The ZnO@ZnS step-scheme photocatalysts had a large surface area,high light utilization capacity,and superior separation efficiency for photogenerated charge carriers.In addition,the material simulation revealed that the formation of the step-scheme heterojunction between ZnO and ZnS was due to the presence of the built-in electric field.Our study paves the way for design of high-performance photocatalysts for H_(2) production.
基金funding from the National Natural Science Foundation of China(No.81703451 and 81773656)the Excellent Youth Science Foundation of Liaoning Province(No.2020-YQ-06)+2 种基金the Liaoning Revitalization Talents Program(No.XLYC1808017 and XLYC1907129)the China Postdoctoral Science Foundation(No.2020M670794)the Science and Technology Major Project of Liaoning(No.2019JH1/10300004)。
文摘Disulfide bond-bridging strategy has been extensively utilized to construct tumor specificity-responsive aliphatic prodrug nanoparticles(PNPs) for precise cancer therapy. Yet, there is no research shedding light on the impacts of the saturation and cis-trans configuration of aliphatic tails on the self-assembly capacity of disulfide bond-linked prodrugs and the in vivo delivery fate of PNPs. Herein, five disulfide bond-linked docetaxelfatty acid prodrugs are designed and synthesized by using stearic acid, elaidic acid, oleic acid, linoleic acid and linolenic acid as the aliphatic tails, respectively. Interestingly, the cistrans configuration of aliphatic tails significantly influences the self-assembly features of prodrugs, and elaidic acid-linked prodrug with a trans double bond show poor self-assembly capacity. Although the aliphatic tails have almost no effect on the redox-sensitive drug release and cytotoxicity, different aliphatic tails significantly influence the chemical stability of prodrugs and the colloidal stability of PNPs, thus affecting the in vivo pharmacokinetics, biodistribution and antitumor efficacy of PNPs. Our findings illustrate how aliphatic tails affect the assembly characteristic of disulfide bond-linked aliphatic prodrugs and the in vivo delivery fate of PNPs, and thus provide theoretical basis for future development of disulfide bond-bridged aliphatic prodrugs.
基金supported by the National Natural Science Foundation of China(Grant Nos.51874362,52072411,51932011)the Natural Science Foundation of Hunan Province(Grant No.2021JJ20060)Open access funding provided by Shanghai Jiao Tong University
文摘Aqueous zinc metal batteries are noted for their costeffectiveness,safety and environmental friendliness.However,the water-induced notorious issues such as continuous electrolyte decomposition and uneven Zn electrochemical deposition remarkably restrict the development of the long-life zinc metal batteries.In this study,zwitterionic sulfobetaine is introduced to copolymerize with acrylamide in zinc perchlorate(Zn(ClO;);)solution.The designed gel framework with hydrophilic and charged groups can firmly anchor water molecules and construct ion migration channels to accelerate ion transport.The in situ generated hybrid interface,which is composed of the organic functionalized outer layer and inorganic Clcontaining inner layer,can synergically lower the mass transfer overpotential,reduce water-related side reactions and lead to uniform Zn deposition.Such a novel electrolyte configuration enables Zn//Zn cells with an ultra-long cycling life of over 3000 h and a low polarization potential(~0.03 V)and Zn//Cu cells with high Coulombic efficiency of 99.18%for 1000 cycles.Full cells matched with MnO;cathodes delivered laudable cycling stability and impressive shelving ability.Besides,the flexible quasi-solid-state batteries which are equipped with the anti-vandalism ability(such as cutting,hammering and soaking)can successfully power the LED simultaneously.Such a safe,processable and durable hydrogel promises significant application potential for long-life flexible electronic devices.
基金supported by the National Science Foundation of China(30672698)
文摘Objective:To study the mechanism of insulin resistance in the cholesterol gallstone formation from insulin signal transduction pathway so as to reveal the possible mechanism and the effective role of Albiflorin Granule on preventing the cholesterol gallstones.Methods:Serum triglycerides(TG),free fatty acid(FFA),and total cholesterol(TC) from different groups were measured and liver cells Ins R,PKB,IKK-β protein expression levels were detected by western blotting.Results:Albiflorin significantly decreased the cholesterol gallstone formation rate,increased glucose infusion rate in gallstone guinea pigs and improved insulin resistance.Compared with the normal group,insulin receptor and PKB protein expression in GS group were significantly reduced.IKK-β protein in the GS group increased significantly and Albiflorin could reduce IKK-β protein expression in guinea pig liver cells.Conclusions:The model of insulin resistance in cholesterol gallstone guinea pig was successfully established,which plays an important role in the cholesterol gallstone formation.All aspects of insulin signaling pathway are involved in gallstone formation.Albiflorin can regulate various aspects of insulin signal transduction pathway to prevent the formation of gallbladder.
文摘Mitochondria are currently known as novel targets for treating cancer,especially for tumors displaying multidrug resistance(MDR). This present study aimed to develop a mitochondriatargeted delivery system by using triphenylphosphonium cation(TPP+)-conjugated Brij 98 as the functional stabilizer to modify paclitaxel(PTX) nanocrystals(NCs) against drugresistant cancer cells. Evaluations were performed on 2 D monolayer and 3 D multicellular spheroids(MCs) of MCF-7 cells and MCF-7/ADR cells. In comparison with free PTX and the non-targeted PTX NCs,the targeted PTX NCs showed the strongest cytotoxicity against both2 D MCF-7 and MCF-7/ADR cells,which was correlated with decreased mitochondrial membrane potential. The targeted PTX NCs exhibited deeper penetration on MCF-7 MCs and more significant growth inhibition on both MCF-7 and MCF-7/ADR MCs. The proposed strategy indicated that the TPP+-modified NCs represent a potentially viable approach for targeted chemotherapeutic molecules to mitochondria. This strategy might provide promising therapeutic outcomes to overcome MDR.
基金financially supported by National Nature Science Foundation of China(No.81872816,81703451)Liaoning Revitalization Talents Program,No XLYC1808017+2 种基金Key projects of Technology bureau in Shenyang,No18400408Key projects of Liaoning Province Department of Education,No.2017LZD03supported by Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region。
文摘One of the major barriers in utilizing prodrug nanocarriers for cancer therapy is the slow release of parent drug in tumors.Tumor cells generally display the higher oxidative level than normal cells,and also displayed the heterogeneity in terms of redox homeostasis level.We previously found that the disulfide bond-linkage demonstrates surprising oxidationsensitivity to form the hydrophilic sulfoxide and sulphone groups.Herein,we develop oxidation-strengthened prodrug nanosystem loaded with pyropheophorbide a(PPa)to achieve light-activatable cascade drug release and enhance therapeutic efficacy.The disulfide bond-driven prodrug nanosystems not only respond to the redox-heterogeneity in tumor,but also respond to the exogenous oxidant(singlet oxygen)elicited by photosensitizers.Once the prodrug nanoparticles(NPs)are activated under irradiation,they would undergo an oxidative self-strengthened process,resulting in a facilitated drug cascade release.The IC50 value of the PPa@PTX-S-S NPs without irradiation was 2-fold higher than those of NPs plus irradiation.In vivo,the PPa@PTX prodrug NPs display prolonged systemic circulation and increased accumulation in tumor site.The PPa@PTXS-S NPs showed much higher efficiency than free PTX or the PPa@PTX-C-C NPs to suppress the growth of 4 T1 tumors.Therefore,this novel oxidation-strengthened disulfide-bridged prodrug-nanosystem has a great potential in the enhanced efficacy of cancer synergetic photochemotherapy.
基金supported by Key R&D Project of Liaoning Province,under Grant Research and Demonstration of Key Technologies for Deep Processing and Comprehensive Utilization of Northeast Hazelnuts(2020JH2/10200037)Service Local Project of Liaoning Province,under Grant Demonstration and Promotion of new deep-processing technology for comprehensive utilization of Northeast Hazelnuts(LSNFW201903)horizontal subject,under Grant Demonstration and Promotion of key technologies for transformation and deep processing of wild hazelnut forest in northwestern Liaoning(H2019388)。
文摘Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.
文摘Photocatalytic hydrogen(H_(2))production via water splitting in the absence of sacrificial agents is a promising strategy for producing clean and sustainable hydrogen energy from solar energy.However,the realization of a photocatalytic pure water splitting system with desirable efficiency is still a huge challenge.Herein,visible light photocatalytic H_(2) production from pure water splitting was successfully achieved using a g-C_(3)N_(4)/CoTiO_(3) S-scheme heterojunction photocatalyst in the absence of sacrificial agents.An optimum hydrogen evolution rate of 118μmol∙h^(−1)∙g^(−1) was reached with the addition of 1.5 wt%CoTiO_(3).The remarkably promoted hydrogen evolution rate was attributed to the intensified light absorption coupled with the synergistic effect of visible light responsive CoTiO_(3),the promoted efficiency in charge separation,and the reserved strong redox capacity induced by the S-scheme charge transfer mechanism.This work provides an alternative to visible light-responding oxidation photocatalysts for the construction of S-scheme heterojunctions and high-efficiency photocatalytic systems for pure water splitting.
