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Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis 被引量:54
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作者 Jin-Ying Gou Ling-Jian Wang +2 位作者 shuang-ping chen Wen-Li Hu Xiao-Ya chen 《Cell Research》 SCIE CAS CSCD 2007年第5期422-434,共13页
Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount of cellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell w... Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount of cellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, we compared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA array identified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA) using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental stages. Similar grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developing fibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulose biosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesis stage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process of cotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has its own unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metabolite profiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis. 展开更多
关键词 cotton fiber TRANSCRIPTOME metabolite profile AUXIN cell elongation cellulose synthesis
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Effect of different bottom blowing elements on stirring characteristics of molten bath in converter
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作者 Ming Lv shuang-ping chen +2 位作者 Hong-min Guo Yi-jie Hao Xiang-dong Xing 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2024年第2期368-376,共9页
The bottom blowing element is the key equipment to ensure the bottom blowing effect of the converter.Three types of bottom blowing elements,dispersive type(D1),double circular seam(D2)and straight cylinder type(D3),we... The bottom blowing element is the key equipment to ensure the bottom blowing effect of the converter.Three types of bottom blowing elements,dispersive type(D1),double circular seam(D2)and straight cylinder type(D3),were built,and the effects of bottom blowing element type on molten bath flow,wall erosion and furnace bottom erosion were simulated.It was found that when the bottom blowing elements of dispersive type(D1)and double circular seam(D2)were used,the dead zone area in the lower part of the molten bath was smaller,and the high-speed zone area was larger;therefore,the stirring effect on the bottom melt was better.When the straight cylinder type(D3)bottom blowing element was used,the gas penetrated the molten bath at a faster rate to reach the surface of molten bath and failed to disperse in the bottom molten bath,and the wall shear stress near the nozzle outlet was larger.When argon was blown by three different bottom blowing elements,the area of the wall shear stress greater than 3 Pa was 4.8,5.6 and 8.7 cm2,respectively,within 0.2 m of the bottom blowing nozzle outlet. 展开更多
关键词 Converter steelmaking Bottom blowing element Molten bath stirring Wall erosion
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