Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was trans...Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.展开更多
Phosphorus concentration on the surface of seawater varies greatly with different environments,especially in coastal.The molecular mechanism by which cyanobacteria adapt to fluctuating phosphorus bioavailability is st...Phosphorus concentration on the surface of seawater varies greatly with different environments,especially in coastal.The molecular mechanism by which cyanobacteria adapt to fluctuating phosphorus bioavailability is still unclear.In this study,transcriptomes and gene knockouts were used to investigate the adaptive molecular mechanism of a model coastal cyanobacterium Synechococcus sp.PCC 7002 during periods of phosphorus starvation and phosphorus recovery(adding sufficient phosphorus after phosphorus starvation).The findings indicated that phosphorus deficiency affected the photosynthesis,ribosome synthesis,and bacterial motility pathways,which recommenced after phosphorus was resupplied.Even more,most of the metabolic pathways of cyanobacteria were enhanced after phosphorus recovery compared to the control which was kept in continuous phosphorus replete conditions.Based on transcriptome,54 genes potentially related to phosphorusdeficiency adaptation were selected and knocked out individually or in combination.It was found that five mutants showed weak growth phenotype under phosphorus deficiency,indicating the importance of the genes(A0076,A0549-50,A1094,A1320,A1895)in the adaptation of phosphorus deficiency.Three mutants were found to grow better than the wild type under phosphorus deficiency,suggesting that the products of these genes(A0079,A0340,A2284–86)might influence the adaptation to phosphorus deficiency.Bioinformatics analysis revealed that cyanobacteria exposed to highly fluctuating phosphorus concentrations have more sophisticated phosphorus acquisition strategies.These results elucidated that Synechococcus sp.PCC 7002 have variable phosphorus response mechanisms to adapt to fluctuating phosphorus concentration,providing a novel perspective of how cyanobacteria may respond to the complex and dynamic environments.展开更多
The Kashan plutons are situated in the central part of Urumieh-Dokhtar magmatic arc recording subduction-related magmatism within the Alpine-Himalayan orogeny in Iran.These rocks consist of different calc-alkaline plu...The Kashan plutons are situated in the central part of Urumieh-Dokhtar magmatic arc recording subduction-related magmatism within the Alpine-Himalayan orogeny in Iran.These rocks consist of different calc-alkaline plutonic rocks including gabbro,gabbroic diorite,microdiorite,monzodiorite,tonalite,granodiorite,and granite.The plutons were emplaced into the Jurassic sedimentary units(Shemshak Formation)and the Eocene calc-alkaline volcanic and pyroclastic rocks.New U-Pb zircon ages show that the Kashan plutons formed during two main periods at 35.20±0.71 Ma in the Late Eocene(Priabonian)and at 18.90±0.84,19.26±0.83,19.30±1.2,and 17.3±1.8 Ma in the Early Miocene(Burdigalian).The reported events in the Kashan plutons imply the final phases of subductionrelated magmatism before the collision which happened between the Arabian and Iranian plates in the Middle Miocene.The plutonic activity in the Kashan region occurred during the transition from Eocene subduction-related setting to Middle Miocene collisional setting.展开更多
基金supported by Sichuan Province Natural Science Foundation of China:(c271628)
文摘Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
基金the support from Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai,No.SML2021SP204)funded by the National Natural Science Founda-tion of China(Grant Nos.32170108,and 42188102)+1 种基金the Science and Technology Innovation 2025 Major Project of Ningbo City(Grant No.2022Z189)the Ningbo Public Welfare Science and Technology Program(Grant Nos.2023S068,2023S040).
文摘Phosphorus concentration on the surface of seawater varies greatly with different environments,especially in coastal.The molecular mechanism by which cyanobacteria adapt to fluctuating phosphorus bioavailability is still unclear.In this study,transcriptomes and gene knockouts were used to investigate the adaptive molecular mechanism of a model coastal cyanobacterium Synechococcus sp.PCC 7002 during periods of phosphorus starvation and phosphorus recovery(adding sufficient phosphorus after phosphorus starvation).The findings indicated that phosphorus deficiency affected the photosynthesis,ribosome synthesis,and bacterial motility pathways,which recommenced after phosphorus was resupplied.Even more,most of the metabolic pathways of cyanobacteria were enhanced after phosphorus recovery compared to the control which was kept in continuous phosphorus replete conditions.Based on transcriptome,54 genes potentially related to phosphorusdeficiency adaptation were selected and knocked out individually or in combination.It was found that five mutants showed weak growth phenotype under phosphorus deficiency,indicating the importance of the genes(A0076,A0549-50,A1094,A1320,A1895)in the adaptation of phosphorus deficiency.Three mutants were found to grow better than the wild type under phosphorus deficiency,suggesting that the products of these genes(A0079,A0340,A2284–86)might influence the adaptation to phosphorus deficiency.Bioinformatics analysis revealed that cyanobacteria exposed to highly fluctuating phosphorus concentrations have more sophisticated phosphorus acquisition strategies.These results elucidated that Synechococcus sp.PCC 7002 have variable phosphorus response mechanisms to adapt to fluctuating phosphorus concentration,providing a novel perspective of how cyanobacteria may respond to the complex and dynamic environments.
基金The TMU Research Grant Council funded field studiespartly supported by grants from the University of Science and Technology of China,HefeiPeking University,Beijing
文摘The Kashan plutons are situated in the central part of Urumieh-Dokhtar magmatic arc recording subduction-related magmatism within the Alpine-Himalayan orogeny in Iran.These rocks consist of different calc-alkaline plutonic rocks including gabbro,gabbroic diorite,microdiorite,monzodiorite,tonalite,granodiorite,and granite.The plutons were emplaced into the Jurassic sedimentary units(Shemshak Formation)and the Eocene calc-alkaline volcanic and pyroclastic rocks.New U-Pb zircon ages show that the Kashan plutons formed during two main periods at 35.20±0.71 Ma in the Late Eocene(Priabonian)and at 18.90±0.84,19.26±0.83,19.30±1.2,and 17.3±1.8 Ma in the Early Miocene(Burdigalian).The reported events in the Kashan plutons imply the final phases of subductionrelated magmatism before the collision which happened between the Arabian and Iranian plates in the Middle Miocene.The plutonic activity in the Kashan region occurred during the transition from Eocene subduction-related setting to Middle Miocene collisional setting.
