The rapid and accurate authentication of traditional Chinese medicines(TCMs)has always been a key scientific and technical problem in the field of pharmaceutical analysis.Herein,a novel heating online extraction elect...The rapid and accurate authentication of traditional Chinese medicines(TCMs)has always been a key scientific and technical problem in the field of pharmaceutical analysis.Herein,a novel heating online extraction electrospray ionization mass spectrometry(H-oEESI-MS)was developed for the rapid and direct analysis of extremely complex substances without the requirement for any sample pretreatment or pre-separation steps.The overall molecular profile and fragment structure features of various herbal medicines could be completely captured within 10–15 s,with minimal sample(<0.5 mg)and solvent consumption(<20μL for one sample).Furthermore,a rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed,including metabolic profile characterization,characteristic marker screening and identification,and multivariate statistical analysis model validation.In an analysis of 52 batches of seven types of Aconitum medicinal materials,20 and 21 key compounds were screened out as the characteristic markers of raw and processed Aconitum herbal medicines,respectively,and the possible structures of all the characteristic markers were comprehensively identified based on Compound Discoverer databases.Finally,multivariate statistical analysis showed that all the different types of herbal medicines were well differentiated and identified(R^(2)X>0.87,R^(2)Y>0.91,and Q^(2)>0.72),which further verified the feasibility and reliability of this comprehensive strategy for the rapid authentication of different TCMs based on H-oEESI-MS.In summary,this rapid authentication strategy realized the ultra-high-throughput,low-cost,and standardized detection of various complex TCMs for the first time,thereby demonstrating wide applicability and value for the development of quality standards for TCMs.展开更多
Remote sensing satellites are playing very important roles in diverse earth observation fields.However,long revisit period,high cost and dense cloud cover have been the main limitations of satellite remote sensing for...Remote sensing satellites are playing very important roles in diverse earth observation fields.However,long revisit period,high cost and dense cloud cover have been the main limitations of satellite remote sensing for a long time.This paper introduces the novel volunteered passenger aircraft remote sensing(VPARS)concept,which can partly overcome these problems.By obtaining aerial imaging data from passengers using a portable smartphone on a passenger aircraft,it has various advantages including low cost,high revisit,dense coverage,and partial anti-cloud,which can well complement conventional remote sensing data.This paper examines the concept of VPARS and give general data processing framework of VPARS.Several cases were given to validate this processing approach.Two preliminary applications on land cover classification and economic activity monitoring validate the applicability of the VPARS data.Furthermore,we examine the issues about data maintenance,potential applications,limitations and challenges.We conclude the VPARS can benefit both scientific and industrial communities who rely on remote sensing data.展开更多
基金supported by the CACMS Innovation Fund,China(Grant Nos.:CI2021A04504 and CI2021A05206)the National Natural Science Foundation of China(Grant Nos.:82104380,81891010,81891013,and 82074012)+2 种基金the Fundamental Research Funds for the Central Public Welfare Research Institutes,China(Grant Nos.:ZZ14-YQ-047 and ZZXT202105)the Key Project at Central Government Level(Grant No.:2060302-2201-26)the Beijing Nova Program.
文摘The rapid and accurate authentication of traditional Chinese medicines(TCMs)has always been a key scientific and technical problem in the field of pharmaceutical analysis.Herein,a novel heating online extraction electrospray ionization mass spectrometry(H-oEESI-MS)was developed for the rapid and direct analysis of extremely complex substances without the requirement for any sample pretreatment or pre-separation steps.The overall molecular profile and fragment structure features of various herbal medicines could be completely captured within 10–15 s,with minimal sample(<0.5 mg)and solvent consumption(<20μL for one sample).Furthermore,a rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed,including metabolic profile characterization,characteristic marker screening and identification,and multivariate statistical analysis model validation.In an analysis of 52 batches of seven types of Aconitum medicinal materials,20 and 21 key compounds were screened out as the characteristic markers of raw and processed Aconitum herbal medicines,respectively,and the possible structures of all the characteristic markers were comprehensively identified based on Compound Discoverer databases.Finally,multivariate statistical analysis showed that all the different types of herbal medicines were well differentiated and identified(R^(2)X>0.87,R^(2)Y>0.91,and Q^(2)>0.72),which further verified the feasibility and reliability of this comprehensive strategy for the rapid authentication of different TCMs based on H-oEESI-MS.In summary,this rapid authentication strategy realized the ultra-high-throughput,low-cost,and standardized detection of various complex TCMs for the first time,thereby demonstrating wide applicability and value for the development of quality standards for TCMs.
基金supported by National Natural Science Foundation of China(41974006)Shenzhen Scientific Research and Development Funding Program(KQJSCX20180328093453763,JCYJ20180305125101282,JCYJ20170412142239369)+1 种基金Open Fund of Key Laboratory of Urban Land Resources Monitoring and Simulation(KF-2018-03-004)Department of Education of Guangdong Province(2018KTSCX196).
文摘Remote sensing satellites are playing very important roles in diverse earth observation fields.However,long revisit period,high cost and dense cloud cover have been the main limitations of satellite remote sensing for a long time.This paper introduces the novel volunteered passenger aircraft remote sensing(VPARS)concept,which can partly overcome these problems.By obtaining aerial imaging data from passengers using a portable smartphone on a passenger aircraft,it has various advantages including low cost,high revisit,dense coverage,and partial anti-cloud,which can well complement conventional remote sensing data.This paper examines the concept of VPARS and give general data processing framework of VPARS.Several cases were given to validate this processing approach.Two preliminary applications on land cover classification and economic activity monitoring validate the applicability of the VPARS data.Furthermore,we examine the issues about data maintenance,potential applications,limitations and challenges.We conclude the VPARS can benefit both scientific and industrial communities who rely on remote sensing data.
