The aim of this study was to evaluate the applicability of Cameriere’s European formula for age estimation in children in South China and to adapt the formula to establish a more suitable formula for these children.M...The aim of this study was to evaluate the applicability of Cameriere’s European formula for age estimation in children in South China and to adapt the formula to establish a more suitable formula for these children.Moreover,the performance of dental age estimation based on Cameriere’s method combining the developmental information of permanent teeth(PT)and third molar(TM)was also analysed.Orthopantomographs of 720 healthy children in Group A,and orthopantomographs of 320 children and 280 subadults in Group B were assessed.The samples of Group A were divided into training dataset 1 and test dataset 1,and the samples of Group B were also divided into training dataset 2 and test dataset 2.A South China-specific formula was established based on the training dataset 1,and the comparison of accuracy between the Cameriere’s European formula and the South China-specific formula was conducted with the test dataset 1.Additionally,a PT regression model,a TM regression model,and a combined regression model(PTþTM)were established based on the training dataset 2,and the performance of these three models were validated on the test dataset 2.The Cameriere’s European formula underestimated chronological age with a mean difference(ME)of-0.47±1.11 years in males and-0.69±1.19 years in females.However,the South China-specific formula underestimated chronological age,with a mean difference(ME)of-0.02±0.71 years in males and-0.14±0.73 years in females.Compared with PT model and TM model,the PT and TM combined model obtained the smallest root mean square error(RMSE)of 1.29 years in males and 0.93 years in females.In conclusion,the South China-specific formula was more suitable for assessing the dental age of children in South China,and the PT and TM combined model can improve the accuracy of dental age estimation in children.展开更多
Goldeneye™DNA ID 22NC Kit is a novel short tandem repeat(STR)genotyping system that investigate 20 non-CODIS loci(D4S2366,D6S477,D22GATA198B05,D15S659,D8S1132,D3S3045,D14S608,D17S1290,D3S1744,D2S441,D18S535,D13S325,D7...Goldeneye™DNA ID 22NC Kit is a novel short tandem repeat(STR)genotyping system that investigate 20 non-CODIS loci(D4S2366,D6S477,D22GATA198B05,D15S659,D8S1132,D3S3045,D14S608,D17S1290,D3S1744,D2S441,D18S535,D13S325,D7S1517,D1OS1435,D11S236&D19S253,D1S1656,D7S3O4&D10S14&and D5S2500),a CODIS locus(D3S1358),and a sex-determining locus amelogenin in one assay.In the present study,this STR genotyping system was validated according to the guidelines of"Wlidation Guidelines for DNA Analysis Methods(2016)"updated by the Scientific Working Group on DNA Analysis Methods.A series of tests,such as polymerase chain reaction-based studies,sensitivity,inhibitors,DNA mixture,species specificity,precision and accuracy evaluation,stutter percentage,and peak height ratio,was conducted.The genetic polymorphism of 21 STR loci that included in the 22NC system was also investigated in the Chinese Hunan Han population.The validation results demonstrated that Goldeneye™DNA ID 22NC Kit is a robust and reliable identification assay as required for genotyping in kinship analysis and forensic investigation.The 21 STR loci in this kit also showed a high level of genetic polymorphism for the Hunan Han population.Therefore,it can be used for forensic applications and population studies.展开更多
文摘The aim of this study was to evaluate the applicability of Cameriere’s European formula for age estimation in children in South China and to adapt the formula to establish a more suitable formula for these children.Moreover,the performance of dental age estimation based on Cameriere’s method combining the developmental information of permanent teeth(PT)and third molar(TM)was also analysed.Orthopantomographs of 720 healthy children in Group A,and orthopantomographs of 320 children and 280 subadults in Group B were assessed.The samples of Group A were divided into training dataset 1 and test dataset 1,and the samples of Group B were also divided into training dataset 2 and test dataset 2.A South China-specific formula was established based on the training dataset 1,and the comparison of accuracy between the Cameriere’s European formula and the South China-specific formula was conducted with the test dataset 1.Additionally,a PT regression model,a TM regression model,and a combined regression model(PTþTM)were established based on the training dataset 2,and the performance of these three models were validated on the test dataset 2.The Cameriere’s European formula underestimated chronological age with a mean difference(ME)of-0.47±1.11 years in males and-0.69±1.19 years in females.However,the South China-specific formula underestimated chronological age,with a mean difference(ME)of-0.02±0.71 years in males and-0.14±0.73 years in females.Compared with PT model and TM model,the PT and TM combined model obtained the smallest root mean square error(RMSE)of 1.29 years in males and 0.93 years in females.In conclusion,the South China-specific formula was more suitable for assessing the dental age of children in South China,and the PT and TM combined model can improve the accuracy of dental age estimation in children.
基金the National Natural Science Foundation of China(No.81302615,81302621,and 81571855).
文摘Goldeneye™DNA ID 22NC Kit is a novel short tandem repeat(STR)genotyping system that investigate 20 non-CODIS loci(D4S2366,D6S477,D22GATA198B05,D15S659,D8S1132,D3S3045,D14S608,D17S1290,D3S1744,D2S441,D18S535,D13S325,D7S1517,D1OS1435,D11S236&D19S253,D1S1656,D7S3O4&D10S14&and D5S2500),a CODIS locus(D3S1358),and a sex-determining locus amelogenin in one assay.In the present study,this STR genotyping system was validated according to the guidelines of"Wlidation Guidelines for DNA Analysis Methods(2016)"updated by the Scientific Working Group on DNA Analysis Methods.A series of tests,such as polymerase chain reaction-based studies,sensitivity,inhibitors,DNA mixture,species specificity,precision and accuracy evaluation,stutter percentage,and peak height ratio,was conducted.The genetic polymorphism of 21 STR loci that included in the 22NC system was also investigated in the Chinese Hunan Han population.The validation results demonstrated that Goldeneye™DNA ID 22NC Kit is a robust and reliable identification assay as required for genotyping in kinship analysis and forensic investigation.The 21 STR loci in this kit also showed a high level of genetic polymorphism for the Hunan Han population.Therefore,it can be used for forensic applications and population studies.
