Objective:Lilium brownii var.viridulum(LB)and L.lancifolium(LL)are the main sources of medicinal lily(Lilii Bulbus,Baihe in Chinese)in China.However,the functional components of these two species responsible for the t...Objective:Lilium brownii var.viridulum(LB)and L.lancifolium(LL)are the main sources of medicinal lily(Lilii Bulbus,Baihe in Chinese)in China.However,the functional components of these two species responsible for the treatment efficacy are yet not clear.In order to explore the therapeutic material basis of Lilii Bulbus,we selected L.davidii var.willmottiae(LD)only used for food as the control group to analyze the differences between LD and the other two(LB and LL).Methods:Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL.Data of metabolome and transcriptome was analysed using various analysis methods,such as principal component analysis(PCA),hierarchical cluster analysis(HCA),and so on.Differentially expressed genes(DEGs)were enriched through KEGG and GO enrichment analysis.Results:The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD.There were 318 significantly differential metabolites(SDMs)in LD vs LB group and 298 SDMs in LD vs LL group.Compared with LD group,the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups,especially in LB group.The HCA of transcriptome indicated that there was significant difference in LB vs LD group,while the difference between LL and LD varied slightly.Additionally,47540 DEGs in LD vs LB group and 18958 DEGs in LD vs LL group were identified.Notably,CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected,and comparing with LD,CYP724,CYP710A,and CYP734A1 in LB and CYP90B in LL were all up-regulated.Conclusion:This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus,which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.展开更多
This study determined the effect of hepatitis B virus （HBV） replication in peripheral blood mononuclear cell （PBMC） from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg- positive moth...This study determined the effect of hepatitis B virus （HBV） replication in peripheral blood mononuclear cell （PBMC） from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg- positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA （rcDNA）, and covalently closed circular DNA （cccDNA） were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi- square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% （12/150） in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cceDNA in the HBsAg- positive mothers were 36.67% （55/150） and 10% （15/150）, respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission （OR = 6.003, 95% Ch 1.249-28.855）. Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA （OR = 3.896, 95% CI： 1.929-7.876） and PBMC HBV cccDNA （OR = 3.74, 95% CI： 1.186--11.793） in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor ofPBMC HBV cccDNA （OR = 0.312, 95% CI： 0.102-0.954） during pregnancy. The positivity ofPBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers （Zz= 5.087, P = 0.024）. This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.展开更多
基金funded by 2022 Provincial Science and Technology Research and Development Plan United Fund(No.222301420075)Henan Provincial High-Level Talents International Training Funding Project(No.2021-72).
文摘Objective:Lilium brownii var.viridulum(LB)and L.lancifolium(LL)are the main sources of medicinal lily(Lilii Bulbus,Baihe in Chinese)in China.However,the functional components of these two species responsible for the treatment efficacy are yet not clear.In order to explore the therapeutic material basis of Lilii Bulbus,we selected L.davidii var.willmottiae(LD)only used for food as the control group to analyze the differences between LD and the other two(LB and LL).Methods:Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL.Data of metabolome and transcriptome was analysed using various analysis methods,such as principal component analysis(PCA),hierarchical cluster analysis(HCA),and so on.Differentially expressed genes(DEGs)were enriched through KEGG and GO enrichment analysis.Results:The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD.There were 318 significantly differential metabolites(SDMs)in LD vs LB group and 298 SDMs in LD vs LL group.Compared with LD group,the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups,especially in LB group.The HCA of transcriptome indicated that there was significant difference in LB vs LD group,while the difference between LL and LD varied slightly.Additionally,47540 DEGs in LD vs LB group and 18958 DEGs in LD vs LL group were identified.Notably,CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected,and comparing with LD,CYP724,CYP710A,and CYP734A1 in LB and CYP90B in LL were all up-regulated.Conclusion:This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus,which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.
文摘This study determined the effect of hepatitis B virus （HBV） replication in peripheral blood mononuclear cell （PBMC） from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg- positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA （rcDNA）, and covalently closed circular DNA （cccDNA） were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi- square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% （12/150） in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cceDNA in the HBsAg- positive mothers were 36.67% （55/150） and 10% （15/150）, respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission （OR = 6.003, 95% Ch 1.249-28.855）. Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA （OR = 3.896, 95% CI： 1.929-7.876） and PBMC HBV cccDNA （OR = 3.74, 95% CI： 1.186--11.793） in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor ofPBMC HBV cccDNA （OR = 0.312, 95% CI： 0.102-0.954） during pregnancy. The positivity ofPBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers （Zz= 5.087, P = 0.024）. This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.
