Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation...Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their in vitro culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use.展开更多
Objective:This study sheds light on the going-out behaviors of patients with chronic diseases when purchasing drugs during COVID-19,explores what factors influence these behaviors,and makes recommendations to guarante...Objective:This study sheds light on the going-out behaviors of patients with chronic diseases when purchasing drugs during COVID-19,explores what factors influence these behaviors,and makes recommendations to guarantee the pharmaceutical treatment of patients with chronic diseases.Methods:A questionnaire based on the health belief model(HBM)was designed and convenience sampling was employed to administer the online questionnaires.The influence of the respondents’demographic information on their going-out behaviors was analyzed by means of multiple linear regression.A structural equation model(SEM)was constructed to explore how health beliefs related to chronic diseases and COVID-19 influenced patients’going-out behaviors.Results:A total of 869 questionnaires were analyzed.The structural equation model revealed that while the severity of the chronic disease had a direct positive effect on going-out behaviors(l=0.106),chronic disease behavior barriers had a direct negative effect on their going-out behaviors(l=-0.204).Conclusion:Educational background,co-living situation,and income could influence patients’going-out behaviors.The severity of the chronic disease and chronic disease behavior barriers directly affected patients’going-out behaviors.Furthermore,patients’health beliefs about COVID-19 ultimately affected their going-out behaviors by influencing their health beliefs related to chronic disease.展开更多
Objective:This study aimed to evaluate the structural reliability and validity of generalized anxiety disorder 7-item(GAD-7)scale in early pregnant women.Methods:In this cross-sectional study,30,823 patients in early ...Objective:This study aimed to evaluate the structural reliability and validity of generalized anxiety disorder 7-item(GAD-7)scale in early pregnant women.Methods:In this cross-sectional study,30,823 patients in early pregnancy registered in the Obstetrics and Gynecology Hospital of Fudan University completed the GAD-7 scale and patient health questionnaire-9 item(PHQ-9).The discriminative ability,reliability,construct validity,and criterion validity were assessed to evaluate the psychometric properties and factor structures.Items with a discrimination parameter(α)of<0.65,factor loading of<0.30,or cross loading of>0.40 in two or more factors simultaneously were deleted from the scale.Results:All GAD-7 scale items exhibited a high discrimination power.The reliability of the GAD-7 scale was good(Cronbach’s alpha coefficient=0.891).Exploratory factor analysis extracted one factor with eigenvalues of greater than 1.0,which explained 61.930%of the common variance.Confirmatory factor analysis confirmed that the one-factor structure fitted the data well.The correlation coefficient with the PHQ-9 was 0.639.Conclusion:The Chinese version of the GAD-7 scale can be used as a screening tool for early pregnant women.It performs well in terms of discriminative ability,reliability,construct validity,and criterion validity.Pregnant women who screen positive may require more attention and investigation to confirm the presence of generalized anxiety disorder.展开更多
基金supported by the Fundamental Research Funds for the Central Universities in China(KYDS201807)Ministry of Science and Technology,China(2016YFE0128500)
文摘Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their in vitro culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use.
文摘Objective:This study sheds light on the going-out behaviors of patients with chronic diseases when purchasing drugs during COVID-19,explores what factors influence these behaviors,and makes recommendations to guarantee the pharmaceutical treatment of patients with chronic diseases.Methods:A questionnaire based on the health belief model(HBM)was designed and convenience sampling was employed to administer the online questionnaires.The influence of the respondents’demographic information on their going-out behaviors was analyzed by means of multiple linear regression.A structural equation model(SEM)was constructed to explore how health beliefs related to chronic diseases and COVID-19 influenced patients’going-out behaviors.Results:A total of 869 questionnaires were analyzed.The structural equation model revealed that while the severity of the chronic disease had a direct positive effect on going-out behaviors(l=0.106),chronic disease behavior barriers had a direct negative effect on their going-out behaviors(l=-0.204).Conclusion:Educational background,co-living situation,and income could influence patients’going-out behaviors.The severity of the chronic disease and chronic disease behavior barriers directly affected patients’going-out behaviors.Furthermore,patients’health beliefs about COVID-19 ultimately affected their going-out behaviors by influencing their health beliefs related to chronic disease.
基金supported by the National Natural Science Foundation of China(82101786)China Medical Board(21-428).
文摘Objective:This study aimed to evaluate the structural reliability and validity of generalized anxiety disorder 7-item(GAD-7)scale in early pregnant women.Methods:In this cross-sectional study,30,823 patients in early pregnancy registered in the Obstetrics and Gynecology Hospital of Fudan University completed the GAD-7 scale and patient health questionnaire-9 item(PHQ-9).The discriminative ability,reliability,construct validity,and criterion validity were assessed to evaluate the psychometric properties and factor structures.Items with a discrimination parameter(α)of<0.65,factor loading of<0.30,or cross loading of>0.40 in two or more factors simultaneously were deleted from the scale.Results:All GAD-7 scale items exhibited a high discrimination power.The reliability of the GAD-7 scale was good(Cronbach’s alpha coefficient=0.891).Exploratory factor analysis extracted one factor with eigenvalues of greater than 1.0,which explained 61.930%of the common variance.Confirmatory factor analysis confirmed that the one-factor structure fitted the data well.The correlation coefficient with the PHQ-9 was 0.639.Conclusion:The Chinese version of the GAD-7 scale can be used as a screening tool for early pregnant women.It performs well in terms of discriminative ability,reliability,construct validity,and criterion validity.Pregnant women who screen positive may require more attention and investigation to confirm the presence of generalized anxiety disorder.
