Potato protease inhibitors(PPIs),as the main component of potato protein isolate,have good safety,nutrition and great market potential.The antioxidant and anticancer properties of PPIs were evaluated with cellbased bi...Potato protease inhibitors(PPIs),as the main component of potato protein isolate,have good safety,nutrition and great market potential.The antioxidant and anticancer properties of PPIs were evaluated with cellbased biological assays.The results showed that when the concentration of PPIs was 5 mg/mL,the peroxyl radical scavenging value was(2119±204)mg VCE/100 g,and the cellular antioxidant activity values were(45.83±3.5)(no PBS wash)and(33.25±4.4)μmol QE/100 g(PBS wash).Cells pretreated with PPIs could significantly prevent the oxidative damage induced by H_(2)O_(2),inhibit the morphological changes of cells and maintain the integrity.Furthermore,PPIs had selective anti-proliferative effects on GIST882 cells(IC50=(10.53±3.87)mg/mL)and demonstrated potent inhibition of the growth,migration and invasion of cancer cells.These findings provide a scientific basis for PPIs as promising candidates for functional foods to aid in the prevention of oxidative damage and cancer.展开更多
This work focused on the role of Al_(2)O_(3) particles in the corrosion behavior of cold sprayed AA5083 aluminum alloy matrix composite(Al-MMC)coatings.The electrochemical characterization of the coatings was investig...This work focused on the role of Al_(2)O_(3) particles in the corrosion behavior of cold sprayed AA5083 aluminum alloy matrix composite(Al-MMC)coatings.The electrochemical characterization of the coatings was investigated in a 3.5 wt.%NaCl solution as a function of time.The results show that fragmentation of Al_(2)O_(3) particles is not clearly observed in the case of AA5083/20 vol.%Al_(2)O_(3) coating,while the broken Al_(2)O_(3) particles can be seen clearly in AA5083/40 vol.%Al_(2)O_(3) and AA5083/60 vol.%Al_(2)O_(3) coatings.The addition of 20 vol.%Al_(2)O_(3) particles yield the coating with the lowest porosity,and different volume fractions of Al_(2)O_(3)in the feedstock have important effects on the electrochemical behavior of composite coatings.The Al-MMC coating reinforced with 20 vol.%Al_(2)O_(3) particles exhibits the highest Ecorrand the lowest icorrcompared with the other conditions.The order of current density is as follows:AA5083/20vol.%Al_(2)O_(3)<AA5083<AA5083/40 vol.%Al_(2)O_(3)<AA5083/60 vol.%Al_(2)O_(3).展开更多
Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COV...Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COVID-19.However,differences in immune responses and clinical features among COVID-19 patients remain largely unknown.Here,we report a database for COVID-19-specific IgG/IgM immune responses and clinical parameters(named COVID-ONE-hi).COVID-ONE-hi is based on the data that contain the IgG/IgM responses to 24 full-length/truncated proteins corresponding to 20 of 28 known SARS-CoV-2 proteins and 199 spike protein peptides against 2360 serum samples collected from 783 COVID-19 patients.In addition,96 clinical parameters for the 2360 serum samples and basic information for the 783 patients are integrated into the database.Furthermore,COVID-ONE-hi provides a dashboard for defining samples and a one-click analysis pipeline for a single group or paired groups.A set of samples of interest is easily defined by adjusting the scale bars of a variety of parameters.After the“START”button is clicked,one can readily obtain a comprehensive analysis report for further interpretation.COVID-ONE-hi is freely available at www.COVID-ONE.cn.展开更多
基金supported by the Science and Technology Mission Project of Liaoning Province Science and Technology Council(2021JH5/10400016)the Service Local Project of Liaoning Provincial Committee of Education(LSNFW202002)the Science and Technology Mission Project of Shenyang Science and Technology Council(20-207-3-25)。
文摘Potato protease inhibitors(PPIs),as the main component of potato protein isolate,have good safety,nutrition and great market potential.The antioxidant and anticancer properties of PPIs were evaluated with cellbased biological assays.The results showed that when the concentration of PPIs was 5 mg/mL,the peroxyl radical scavenging value was(2119±204)mg VCE/100 g,and the cellular antioxidant activity values were(45.83±3.5)(no PBS wash)and(33.25±4.4)μmol QE/100 g(PBS wash).Cells pretreated with PPIs could significantly prevent the oxidative damage induced by H_(2)O_(2),inhibit the morphological changes of cells and maintain the integrity.Furthermore,PPIs had selective anti-proliferative effects on GIST882 cells(IC50=(10.53±3.87)mg/mL)and demonstrated potent inhibition of the growth,migration and invasion of cancer cells.These findings provide a scientific basis for PPIs as promising candidates for functional foods to aid in the prevention of oxidative damage and cancer.
基金financially supported by the authors would like to acknowledge the National Key Research and Development Program of China(No.2016YFB1100104)the National Natural Science Foundation of China(No.51875470)+1 种基金the State Key Laboratory of Solidification Processing(NPU,China)(No.2019-QZ-01)the financial support from the fund of SAST(No.SAST2016043)。
文摘This work focused on the role of Al_(2)O_(3) particles in the corrosion behavior of cold sprayed AA5083 aluminum alloy matrix composite(Al-MMC)coatings.The electrochemical characterization of the coatings was investigated in a 3.5 wt.%NaCl solution as a function of time.The results show that fragmentation of Al_(2)O_(3) particles is not clearly observed in the case of AA5083/20 vol.%Al_(2)O_(3) coating,while the broken Al_(2)O_(3) particles can be seen clearly in AA5083/40 vol.%Al_(2)O_(3) and AA5083/60 vol.%Al_(2)O_(3) coatings.The addition of 20 vol.%Al_(2)O_(3) particles yield the coating with the lowest porosity,and different volume fractions of Al_(2)O_(3)in the feedstock have important effects on the electrochemical behavior of composite coatings.The Al-MMC coating reinforced with 20 vol.%Al_(2)O_(3) particles exhibits the highest Ecorrand the lowest icorrcompared with the other conditions.The order of current density is as follows:AA5083/20vol.%Al_(2)O_(3)<AA5083<AA5083/40 vol.%Al_(2)O_(3)<AA5083/60 vol.%Al_(2)O_(3).
基金partially supported by the National Key R&D Program of China Grant(Grant No.2016YFA0500600)the National Natural Science Foundation of China(Grant Nos.31970130,31600672,31900112,21907065,and 32000027)。
文摘Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COVID-19.However,differences in immune responses and clinical features among COVID-19 patients remain largely unknown.Here,we report a database for COVID-19-specific IgG/IgM immune responses and clinical parameters(named COVID-ONE-hi).COVID-ONE-hi is based on the data that contain the IgG/IgM responses to 24 full-length/truncated proteins corresponding to 20 of 28 known SARS-CoV-2 proteins and 199 spike protein peptides against 2360 serum samples collected from 783 COVID-19 patients.In addition,96 clinical parameters for the 2360 serum samples and basic information for the 783 patients are integrated into the database.Furthermore,COVID-ONE-hi provides a dashboard for defining samples and a one-click analysis pipeline for a single group or paired groups.A set of samples of interest is easily defined by adjusting the scale bars of a variety of parameters.After the“START”button is clicked,one can readily obtain a comprehensive analysis report for further interpretation.COVID-ONE-hi is freely available at www.COVID-ONE.cn.
