AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were colle...AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups:benign ascites(n=21)and malignant ascites(n=15).Benign ascites included cirrhotic ascites(n=10)and tuberculous ascites(n=5).Malignant ascites included oophoroma(n=7),cancer of colon(n=5),cancer of the liver(n=6),gastric cancer(n=2),and bladder carcinoma(n=1).The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction(RT-PCR)technology,and the positive rate of COX-2 mRNA was compared between different diseases.RESULTS:The positive rate of COX-2 mRNA in malignant ascites was 42.9%(9/21),which was significantly higher than in benign ascites,6.7%(1/15),difference being significant between these two groups(χ2=4.051,P=0.044).The proportion of the positive rate in the malignant ascites was as follows:ovarian cancers57.1%(4/7),colon cancer 40.0%(2/5),liver cancer33.3%(2/6),gastric cancer 50.0%(1/2),and bladder cancer 0.00%(0/1).However,there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites(χ2=1.614,P=0.806).Among the benign ascites,COX-2 mRNA levels were different between the tuberculous ascites(0/5)and cirrhotic ascites(1/10),but there was no significant difference(P=1.000).CONCLUSION:COX-2 mRNA,detected by RT-PCR,is useful in the differential diagnosis of benign and malignant ascites,which also has potential value in the clinical diagnosis of tumors.展开更多
Objective:To discuss effect of ionizing radiation on transcription of colorectal cancer multidrug resistance(MDR) 1 gene of HCT-8 cells.Methods:Total RNA was extracted by guanidine thiocyanate one-step method.Northern...Objective:To discuss effect of ionizing radiation on transcription of colorectal cancer multidrug resistance(MDR) 1 gene of HCT-8 cells.Methods:Total RNA was extracted by guanidine thiocyanate one-step method.Northern blot was applied to detect transcription level of MDR1 gene.The expression of P-gp protein was detected by flow cytometry.Results:The expression of MDRl of normal colorectal cancer HCT-8 cells was low.It was increased by 8.35 times under stimulus with 2 Gy.When treated with low doses in advance,high expressed MDR was decreased significantly under 0.05,0.1 Gy,which was 69.00%,62.89%in 2 Cy group and 5.77 times,5.25 times in sham irradiation group.No obvious difference was detected between(0.2+2) Gy group and 2 Gy group.Compared with sham irradiation group,the percentage of P-gp positive cells after radiation of a high 2 Gy dose was increased significantly(P【0.01).When treated with high radiation dose following low radiation dose(0.05 Gy,0.1 Gy) in advance,the percentage of P-gp positive cells were also increased significantly.The percentage of P-gp positive cells were increased obviously in 0.2 Gy and 2 Gy groups.Compared with simple high radiation 2 Gy group,the percentage of P-gp positive cells was decreased significantly(P【0.05).Conclusions: Low radiation dose can reverse multidrug resistance of colorectal cancer cells caused by high radiation dose.展开更多
基金Supported by Fund of Science and Technology Plan Project in Zhuhai,No.PC20061084
文摘AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups:benign ascites(n=21)and malignant ascites(n=15).Benign ascites included cirrhotic ascites(n=10)and tuberculous ascites(n=5).Malignant ascites included oophoroma(n=7),cancer of colon(n=5),cancer of the liver(n=6),gastric cancer(n=2),and bladder carcinoma(n=1).The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction(RT-PCR)technology,and the positive rate of COX-2 mRNA was compared between different diseases.RESULTS:The positive rate of COX-2 mRNA in malignant ascites was 42.9%(9/21),which was significantly higher than in benign ascites,6.7%(1/15),difference being significant between these two groups(χ2=4.051,P=0.044).The proportion of the positive rate in the malignant ascites was as follows:ovarian cancers57.1%(4/7),colon cancer 40.0%(2/5),liver cancer33.3%(2/6),gastric cancer 50.0%(1/2),and bladder cancer 0.00%(0/1).However,there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites(χ2=1.614,P=0.806).Among the benign ascites,COX-2 mRNA levels were different between the tuberculous ascites(0/5)and cirrhotic ascites(1/10),but there was no significant difference(P=1.000).CONCLUSION:COX-2 mRNA,detected by RT-PCR,is useful in the differential diagnosis of benign and malignant ascites,which also has potential value in the clinical diagnosis of tumors.
文摘Objective:To discuss effect of ionizing radiation on transcription of colorectal cancer multidrug resistance(MDR) 1 gene of HCT-8 cells.Methods:Total RNA was extracted by guanidine thiocyanate one-step method.Northern blot was applied to detect transcription level of MDR1 gene.The expression of P-gp protein was detected by flow cytometry.Results:The expression of MDRl of normal colorectal cancer HCT-8 cells was low.It was increased by 8.35 times under stimulus with 2 Gy.When treated with low doses in advance,high expressed MDR was decreased significantly under 0.05,0.1 Gy,which was 69.00%,62.89%in 2 Cy group and 5.77 times,5.25 times in sham irradiation group.No obvious difference was detected between(0.2+2) Gy group and 2 Gy group.Compared with sham irradiation group,the percentage of P-gp positive cells after radiation of a high 2 Gy dose was increased significantly(P【0.01).When treated with high radiation dose following low radiation dose(0.05 Gy,0.1 Gy) in advance,the percentage of P-gp positive cells were also increased significantly.The percentage of P-gp positive cells were increased obviously in 0.2 Gy and 2 Gy groups.Compared with simple high radiation 2 Gy group,the percentage of P-gp positive cells was decreased significantly(P【0.05).Conclusions: Low radiation dose can reverse multidrug resistance of colorectal cancer cells caused by high radiation dose.
