The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals.Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathog...The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals.Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis,the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive.Here,we utilized a novel force tracing technique based on atomic force microscopy(AFM)to record the process of single viral entry into host cell.We revealed that the force of the rabies virus internalization distributed at(65±25)pN,and the time was identified by two peaks with spacings of(237.2±59.1)and(790.3±134.4)ms with the corresponding speed of 0.12 and 0.04µm/s,respectively.Our results provide insight into the effects of viral shape during the endocytosis process.This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.展开更多
基金This work was supported by the Project of the Education Department of Jilin Province,China(No.JJKH20220666KJ)the Talent of Jilin Province Development Fund Project,China(No.2021Y004)National Natural Science Foundation of China(No.21773017).
文摘The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals.Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis,the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive.Here,we utilized a novel force tracing technique based on atomic force microscopy(AFM)to record the process of single viral entry into host cell.We revealed that the force of the rabies virus internalization distributed at(65±25)pN,and the time was identified by two peaks with spacings of(237.2±59.1)and(790.3±134.4)ms with the corresponding speed of 0.12 and 0.04µm/s,respectively.Our results provide insight into the effects of viral shape during the endocytosis process.This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.
