A novel approach is proposed towards the design of fiber Bragg gratings with multi-channel right-angled triangular spectrum.Firstly,a single-channel grating is synthesized utilizing an adaptive quantum particle swarm ...A novel approach is proposed towards the design of fiber Bragg gratings with multi-channel right-angled triangular spectrum.Firstly,a single-channel grating is synthesized utilizing an adaptive quantum particle swarm optimization with the piecewise constant mutated factor.Meanwhile,the reflectivity spectrum with good linear edge for a short grating is obtained.Then,for its merits of easy fabrication,the superposition method is adopted to design multi-channel gratings with initial spectral distortion.Finally,this distortion is optimized by the method in the first step.It is shown that the design outcomes still retain the features of easy fabrication and short length.Such gratings would be useful as wavelength-interrogation devices with multiple physical parameters in optical sensor systems.展开更多
目的构建Flag-葡萄球菌蛋白A(staphylococcal protein A,SPA)与转座酶Tn5基因的重组原核表达质粒p TXB1-Flag-pA-Tn5,在大肠埃希菌中表达、纯化重组Flag-pA-Tn5蛋白,并检测其转座酶活性。方法合成Flag-pA基因的DNA编码序列,PCR扩增后插...目的构建Flag-葡萄球菌蛋白A(staphylococcal protein A,SPA)与转座酶Tn5基因的重组原核表达质粒p TXB1-Flag-pA-Tn5,在大肠埃希菌中表达、纯化重组Flag-pA-Tn5蛋白,并检测其转座酶活性。方法合成Flag-pA基因的DNA编码序列,PCR扩增后插入原核表达载体pTXB1-Tn5,构建重组原核表达质粒pTXB1-Flag-pA-Tn5;转化大肠埃希菌BL21(DE3),IPTG诱导表达带有Flag标签的pA-Tn5蛋白,并经几丁质树脂亲和纯化后透析复性;将Flag-p A-Tn5蛋白与含测序引物接头的转座子DNA片段组装成转座体,取不同稀释比例的转座体与人外周血白细胞基因组DNA共孵育,以DNA孵育产物为模板、测序引物进行PCR扩增,鉴定Flag-pA-Tn5转座酶活性。结果重组原核表达质粒pTXB1-Flag-pA-Tn5经双酶切和测序证明构建正确;表达的重组蛋白产量为211.1μg/mL,能够被几丁质树脂亲和纯化,纯度为84%;Flag-pA-Tn5蛋白与转座子DNA片段组装后,能有效切割人外周血白细胞基因组DNA,并连接测序引物接头,具有转座酶活性。结论获得了具有转座酶活性的重组Flag-pA-Tn5蛋白,为进一步利用Flag-p A-Tn5转座酶进行基因功能组学研究奠定了基础。展开更多
Background Twist is a highly conserved epithelial-mesenchymal transcription factor that has been reported to be a key factor in tumor malignancy, including lymph node metastasis. It represents the major step of dissem...Background Twist is a highly conserved epithelial-mesenchymal transcription factor that has been reported to be a key factor in tumor malignancy, including lymph node metastasis. It represents the major step of dissemination and serves as a chief prognostic indicator of disease progression. However, the mechanism by which Twist regulates lymph node metastasis remains incompletely understood. Studies on the mechanism of metastasis are thus required for determining appropriate therapeutic strategies.Methods Immunohistochemistry for lymphatic vessel endothelial receptor 1 （LYVE-1）, Ki-67, Twist, vascular endothelial growth factor C （VEGF-C）, and vascular endothelial growth factor receptor 3 （VEGFR-3） was performed to detect lymphatic vessel density （LVD）, cell proliferation levels and the expressions of Twist, VEGF-C, and VEGFR-3 were determined from 66 primary supraglottic carcinoma tissue samples from 36 patients with lymph node metastasis （pathological N＋, pN＋） and 30 patients without metastasis （pathological NO, pNO）. Western blotting analysis of the proteins in pN＋ and pNO primary tumors was used to characterize the expressions of Twist, VEGF-C, and VEGFR-3further.Results The LVD was 22.4±10.3 in pN＋ patients and 6.8±4.1 in pNO ones. For Ki-67, the number of proliferous cells in pN＋ patients was greater than that in pNO ones. Both, however, were associated with their clinical nodal stages. In pN＋patients, Twist, VEGF-C, and VEGFR-3 expressions were 86.11% （31/36）, 80.56% （29/36）, and 58.33% （21/36）,respectively. These values were higher than those found for pNO patients （i.e., 13/30, 11/30, and 7/30, respectively） （P 〈0.05）. Among the samples with Twist expression, 88.64% were VEGF-C-positive and 59.09% were VEGFR-3-positive.The pNO counterparts were 4.55% and 9.09%, respectively （P〈0.05）. The expressions of Twist, VEGF-C, and VEGFR-3in pN＋ patients obtained through Western blotting analysis were significantly higher than those in pNO patients, and the levels of VEGF-C and VEGFR-3 were positively correlated with that of Twist.Conclusions Twist expression correlates with lymph node metastasis. The mechanism involved in such a correlation may be related to lymphangiogenesis.展开更多
基金Supported by National Science Foundation of China under Grant Nos 60977034,61107036,11004043,11274083in part by China Postdoctoral Science Foundation under Grant Nos 20110491092,2012T50354.
文摘A novel approach is proposed towards the design of fiber Bragg gratings with multi-channel right-angled triangular spectrum.Firstly,a single-channel grating is synthesized utilizing an adaptive quantum particle swarm optimization with the piecewise constant mutated factor.Meanwhile,the reflectivity spectrum with good linear edge for a short grating is obtained.Then,for its merits of easy fabrication,the superposition method is adopted to design multi-channel gratings with initial spectral distortion.Finally,this distortion is optimized by the method in the first step.It is shown that the design outcomes still retain the features of easy fabrication and short length.Such gratings would be useful as wavelength-interrogation devices with multiple physical parameters in optical sensor systems.
文摘Background Twist is a highly conserved epithelial-mesenchymal transcription factor that has been reported to be a key factor in tumor malignancy, including lymph node metastasis. It represents the major step of dissemination and serves as a chief prognostic indicator of disease progression. However, the mechanism by which Twist regulates lymph node metastasis remains incompletely understood. Studies on the mechanism of metastasis are thus required for determining appropriate therapeutic strategies.Methods Immunohistochemistry for lymphatic vessel endothelial receptor 1 （LYVE-1）, Ki-67, Twist, vascular endothelial growth factor C （VEGF-C）, and vascular endothelial growth factor receptor 3 （VEGFR-3） was performed to detect lymphatic vessel density （LVD）, cell proliferation levels and the expressions of Twist, VEGF-C, and VEGFR-3 were determined from 66 primary supraglottic carcinoma tissue samples from 36 patients with lymph node metastasis （pathological N＋, pN＋） and 30 patients without metastasis （pathological NO, pNO）. Western blotting analysis of the proteins in pN＋ and pNO primary tumors was used to characterize the expressions of Twist, VEGF-C, and VEGFR-3further.Results The LVD was 22.4±10.3 in pN＋ patients and 6.8±4.1 in pNO ones. For Ki-67, the number of proliferous cells in pN＋ patients was greater than that in pNO ones. Both, however, were associated with their clinical nodal stages. In pN＋patients, Twist, VEGF-C, and VEGFR-3 expressions were 86.11% （31/36）, 80.56% （29/36）, and 58.33% （21/36）,respectively. These values were higher than those found for pNO patients （i.e., 13/30, 11/30, and 7/30, respectively） （P 〈0.05）. Among the samples with Twist expression, 88.64% were VEGF-C-positive and 59.09% were VEGFR-3-positive.The pNO counterparts were 4.55% and 9.09%, respectively （P〈0.05）. The expressions of Twist, VEGF-C, and VEGFR-3in pN＋ patients obtained through Western blotting analysis were significantly higher than those in pNO patients, and the levels of VEGF-C and VEGFR-3 were positively correlated with that of Twist.Conclusions Twist expression correlates with lymph node metastasis. The mechanism involved in such a correlation may be related to lymphangiogenesis.
