The diversity of prokaryotic communities in soil is shaped by both biotic and abiotic factors.However,little is known about the major factors shaping soil prokaryotic communities at a large scale in agroecosystems.To ...The diversity of prokaryotic communities in soil is shaped by both biotic and abiotic factors.However,little is known about the major factors shaping soil prokaryotic communities at a large scale in agroecosystems.To this end,we undertook a study to investigate the impact of maize production cropping systems,soil properties and geographic location(latitude and longitude)on soil prokaryotic communities using metagenomic techniques,across four distinct maize production regions in China.Across all study sites,the dominant prokaryotes in soil were Alphaproteobacteria,Gammaproteobacteria,Betaproteobacteria,Gemmatimonadetes,Acidobacteria,and Actinobacteria.Non-metric multidimensional scaling revealed that prokaryotic communities clustered into the respective maize cropping systems in which they resided.Redundancy analysis(RDA)showed that soil properties especially pH,geographic location and cropping system jointly determined the diversity of the prokaryotic communities.The functional genes of soil prokaryotes from these samples were chiefly influenced by latitude,soil pH and cropping system,as revealed by RDA analysis.The abundance of genes in some metabolic pathways,such as genes involved in microbe–microbe interactions,degradation of aromatic compounds,carbon fixation pathways in prokaryotes and microbial metabolism were markedly different across the four maize production regions.Our study indicated that the combination of soil pH,cropping system and geographic location significantly influenced the prokaryotic community and the functional genes of these microbes.This work contributes to a deeper understanding of the composition and function of the soil prokaryotic community across large-scale production systems such as maize.展开更多
The expression of mitogen-activated protein kinase(mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However,little is known about the ecol...The expression of mitogen-activated protein kinase(mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However,little is known about the ecological effects of transgenic plants. Here,we analyzed the diversity of 16 S r DNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change. A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk ds RNA-expressing plants,respectively. No significant difference was observed between the two groups according to Shannon and Simpson indices. In soil samples of the two rhizospheres,the dominant group was Crenarchaeota at the phylum level,with Staphylothermus,Methanococcus,Pyrodictium and Sulfolobus the abundant taxa at the genus level. These results suggest that expressing mapk double-stranded(ds) RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea,and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk ds RNA.展开更多
基金supported by the National Program for Support of Top-notch Young Professionals,Chinathe Open Research Fund of State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences(SKLQF201508)the Project of Plant Protection Key Discipline of Henan Province,China。
文摘The diversity of prokaryotic communities in soil is shaped by both biotic and abiotic factors.However,little is known about the major factors shaping soil prokaryotic communities at a large scale in agroecosystems.To this end,we undertook a study to investigate the impact of maize production cropping systems,soil properties and geographic location(latitude and longitude)on soil prokaryotic communities using metagenomic techniques,across four distinct maize production regions in China.Across all study sites,the dominant prokaryotes in soil were Alphaproteobacteria,Gammaproteobacteria,Betaproteobacteria,Gemmatimonadetes,Acidobacteria,and Actinobacteria.Non-metric multidimensional scaling revealed that prokaryotic communities clustered into the respective maize cropping systems in which they resided.Redundancy analysis(RDA)showed that soil properties especially pH,geographic location and cropping system jointly determined the diversity of the prokaryotic communities.The functional genes of soil prokaryotes from these samples were chiefly influenced by latitude,soil pH and cropping system,as revealed by RDA analysis.The abundance of genes in some metabolic pathways,such as genes involved in microbe–microbe interactions,degradation of aromatic compounds,carbon fixation pathways in prokaryotes and microbial metabolism were markedly different across the four maize production regions.Our study indicated that the combination of soil pH,cropping system and geographic location significantly influenced the prokaryotic community and the functional genes of these microbes.This work contributes to a deeper understanding of the composition and function of the soil prokaryotic community across large-scale production systems such as maize.
基金supported by the National Natural Science Foundation of China (30900926)funded by the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS)the China Agriculture Research System (CARS-23-D01)
文摘The expression of mitogen-activated protein kinase(mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However,little is known about the ecological effects of transgenic plants. Here,we analyzed the diversity of 16 S r DNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change. A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk ds RNA-expressing plants,respectively. No significant difference was observed between the two groups according to Shannon and Simpson indices. In soil samples of the two rhizospheres,the dominant group was Crenarchaeota at the phylum level,with Staphylothermus,Methanococcus,Pyrodictium and Sulfolobus the abundant taxa at the genus level. These results suggest that expressing mapk double-stranded(ds) RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea,and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk ds RNA.
