Objective To investigate the effect of low concentration of Wenyang Tonglin Decoction(WTD)on the binding conditions of R45 plasmid conjugative transfer under liquid phase conjugation and its mechanism.Methods Escheric...Objective To investigate the effect of low concentration of Wenyang Tonglin Decoction(WTD)on the binding conditions of R45 plasmid conjugative transfer under liquid phase conjugation and its mechanism.Methods Escherichia coli CP9(R45)and Staphylococcus aureus RN450RF were cultured in medium containing WTD,and their minimum inhibitory concentration(MIC)values were obtained.Using promoter fusion technology,E.coli CP9(R45)containing a promoter fusion was obtained.β-Galactosidase activity of TrfAp and TrbBp was tested,and the mRNA expression of regulatory factors(TrbA,KorA,and KorB)was detected by real-time fluorescent quantitative polymerase chain reaction.Results The MIC of E.coli CP9(R45)was 400 g/L and that of S.aureus RN450RF was 200 g/L.When the drug concentration in the culture medium was 200 g/L,the highest number of conjugants was(3.47±0.20)×10^(7) CFU/mL At 90 h of conjugation,the maximum number of conjugants was(1.15±0.06)×10^(8) CFU/mL When the initial bacterial concentration was 10^(8) CFU/mL,the maximum number of conjugants was(3.47±0.20)×10^(7) CFU/mL.When the drug concentration was 200 g/L,theβ-galactosidase activity of TrfAp and TrbBp significantly increased;the relative quantification of TrbA,KorA and KorB were significantly inhibited.Conclusion Low concentration of WTD promoted the development of bacterial resistance by affecting promoters and inhibiting the expression of regulatory factors.展开更多
Objective： To observe the alteration of QT dispersion（QTd） and QTc dispersion（QTcd） in hemodialysis patients after oral administration of Zhigancao Decoction（炙甘草汤,Roasted Licorice Decoction,RLD）.Methods： T...Objective： To observe the alteration of QT dispersion（QTd） and QTc dispersion（QTcd） in hemodialysis patients after oral administration of Zhigancao Decoction（炙甘草汤,Roasted Licorice Decoction,RLD）.Methods： To investigate the alteration of QTd and QTcd in 68 routine hemodialysis patients before and after hemodialysis with 12-lead electrocardiogram（ECG） after orally administrated RLD for 4 weeks.Blood was also taken for measurement of plasma electrolytes,liver function,renal function,hemoglobin（Hgb） and hematocrit（HCT）.Results： After hemodialysis,QTd and QTcd were prolonged evidently; the difference was significant between before and after hemodialysis（P〈0.05）.After RLD orally administrated for 4 weeks,QTd and QTcd only slightly increased after dialysis compared with pre-dialysis（P〉0.05）.The QTd and QTcd of the post-therapy-post-dialysis decreased significantly compared with the pre-therapy-post-dialysis（P〈0.05）.There were no other significant changes in other variables（post-therapy-pre-dialysis vs.pre-therapy-pre-dialysis,or post-therapy-post-dialysis vs.pre-therapy-post-dialysis; P〉0.05）.After therapy,the number of patients with supraventricular arrhythmia,occasional ventricular premature beat and multiple ventricular premature beat were decreased from 15 to 4,10 to 2 and 7 to 1,respectively.Conclusion： RLD therapy not only lowered the increased QTd and QTcd after hemodialysis,but also displayed a safety profile.展开更多
基金Supported by the National Natural Science Foundation of China(No.81873070)。
文摘Objective To investigate the effect of low concentration of Wenyang Tonglin Decoction(WTD)on the binding conditions of R45 plasmid conjugative transfer under liquid phase conjugation and its mechanism.Methods Escherichia coli CP9(R45)and Staphylococcus aureus RN450RF were cultured in medium containing WTD,and their minimum inhibitory concentration(MIC)values were obtained.Using promoter fusion technology,E.coli CP9(R45)containing a promoter fusion was obtained.β-Galactosidase activity of TrfAp and TrbBp was tested,and the mRNA expression of regulatory factors(TrbA,KorA,and KorB)was detected by real-time fluorescent quantitative polymerase chain reaction.Results The MIC of E.coli CP9(R45)was 400 g/L and that of S.aureus RN450RF was 200 g/L.When the drug concentration in the culture medium was 200 g/L,the highest number of conjugants was(3.47±0.20)×10^(7) CFU/mL At 90 h of conjugation,the maximum number of conjugants was(1.15±0.06)×10^(8) CFU/mL When the initial bacterial concentration was 10^(8) CFU/mL,the maximum number of conjugants was(3.47±0.20)×10^(7) CFU/mL.When the drug concentration was 200 g/L,theβ-galactosidase activity of TrfAp and TrbBp significantly increased;the relative quantification of TrbA,KorA and KorB were significantly inhibited.Conclusion Low concentration of WTD promoted the development of bacterial resistance by affecting promoters and inhibiting the expression of regulatory factors.
基金Supported by the National Natural Science Foundation of China(No.81374039)
文摘Objective： To observe the alteration of QT dispersion（QTd） and QTc dispersion（QTcd） in hemodialysis patients after oral administration of Zhigancao Decoction（炙甘草汤,Roasted Licorice Decoction,RLD）.Methods： To investigate the alteration of QTd and QTcd in 68 routine hemodialysis patients before and after hemodialysis with 12-lead electrocardiogram（ECG） after orally administrated RLD for 4 weeks.Blood was also taken for measurement of plasma electrolytes,liver function,renal function,hemoglobin（Hgb） and hematocrit（HCT）.Results： After hemodialysis,QTd and QTcd were prolonged evidently; the difference was significant between before and after hemodialysis（P〈0.05）.After RLD orally administrated for 4 weeks,QTd and QTcd only slightly increased after dialysis compared with pre-dialysis（P〉0.05）.The QTd and QTcd of the post-therapy-post-dialysis decreased significantly compared with the pre-therapy-post-dialysis（P〈0.05）.There were no other significant changes in other variables（post-therapy-pre-dialysis vs.pre-therapy-pre-dialysis,or post-therapy-post-dialysis vs.pre-therapy-post-dialysis; P〉0.05）.After therapy,the number of patients with supraventricular arrhythmia,occasional ventricular premature beat and multiple ventricular premature beat were decreased from 15 to 4,10 to 2 and 7 to 1,respectively.Conclusion： RLD therapy not only lowered the increased QTd and QTcd after hemodialysis,but also displayed a safety profile.
