Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information r...Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m^(6)A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m^(6)A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m^(6)A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m^(6)A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m^(6)A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m^(6)A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m^(6)A modification in E.tenella,which provides significant insights into developmental biology of E.tenella.展开更多
Purpose:The purpose of this study is to propose an improved credit allocation method that makes the leading author of the paper more distinguishable and makes the deification more robust under malicious manipulations....Purpose:The purpose of this study is to propose an improved credit allocation method that makes the leading author of the paper more distinguishable and makes the deification more robust under malicious manipulations.Design/methodology/approach:We utilize a modified Sigmoid function to handle the fat-tail distributed citation counts.We also remove the target paper in calculating the contribution of co-citations.Following previous studies,we use 30 Nobel Prize-winning papers and their citation networks based on the American Physical Society(APS)and the Microsoft Academic Graph(MAG)dataset to test the accuracy of our proposed method(NCCAS).In addition,we use 654,148 articles published in the field of computer science from 2000 to 2009 in the MAG dataset to validate the distinguishability and robustness of NCCAS.Finding:Compared with the state-of-the-art methods,NCCAS gives the most accurate prediction of Nobel laureates.Furthermore,the leading author of the paper identified by NCCAS is more distinguishable compared with other co-authors.The results by NCCAS are also more robust to malicious manipulation.Finally,we perform ablation studies to show the contribution of different components in our methods.Research limitations:Due to limited ground truth on the true leading author of a work,the accuracy of NCCAS and other related methods can only be tested in Nobel Physics Prize-winning papers.Practical implications:NCCAS is successfully applied to a large number of publications,demonstrating its potential in analyzing the relationship between the contribution and the recognition of authors with different by-line orders.Originality/value:Compared with existing methods,NCCAS not only identifies the leading author of a paper more accurately,but also makes the deification more distinguishable and more robust,providing a new tool for related studies.展开更多
As an important part of Chinese excellent traditional culture,Jingchu culture has profound historical and cultural value.The exploration and application of Jingchu culture elements is conducive to carrying forward and...As an important part of Chinese excellent traditional culture,Jingchu culture has profound historical and cultural value.The exploration and application of Jingchu culture elements is conducive to carrying forward and inheriting history and culture and enriching urban cultural deposits.Theme park is an important place to spread and promote Jingchu culture.It is of far-reaching significance to study and explore the integration of Jingchu culture elements into the construction of theme park.This paper analyzes the concept and connotation of Jingchu culture,combs and analyzes the extraction content of Jingchu culture elements,and summarizes the expression pathways of Jingchu culture elements in theme park and the practical application in Jingzhou Fantawild,in order to provide certain inspiration and reference for contemporary landscape designers to better inherit and develop Jingchu culture elements.展开更多
Sunshine Sports has played a significant role in promoting the teaching of Taekwondo in colleges and universities.By analyzing the connection between the Sunshine Sports concept and Taekwondo teaching,the characterist...Sunshine Sports has played a significant role in promoting the teaching of Taekwondo in colleges and universities.By analyzing the connection between the Sunshine Sports concept and Taekwondo teaching,the characteristics of the Sunshine Sports concept in Taekwondo teaching in colleges and universities are discussed.This article expounds on the influencing factors in implementing the Sunshine Sports concept and discusses how to carry out scientific and reasonable teaching methods.展开更多
基金supported by the National Natural Science Foundation of China(31902298)the Shanxi Provincial Key Research and Development Program,China(2022ZDYF126)+2 种基金the Fund for Shanxi“1331 Project”,China(20211331-13)the Science and Technology Innovation Program of Shanxi Agricultural University,China(2017YJ10)the Special Research Fund of Shanxi Agricultural University for High-level Talents,China(2021XG001)。
文摘Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m^(6)A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m^(6)A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m^(6)A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m^(6)A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m^(6)A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m^(6)A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m^(6)A modification in E.tenella,which provides significant insights into developmental biology of E.tenella.
基金This work was supported by University Innovation Research Group of Chongqing(No.CXQT21005).
文摘Purpose:The purpose of this study is to propose an improved credit allocation method that makes the leading author of the paper more distinguishable and makes the deification more robust under malicious manipulations.Design/methodology/approach:We utilize a modified Sigmoid function to handle the fat-tail distributed citation counts.We also remove the target paper in calculating the contribution of co-citations.Following previous studies,we use 30 Nobel Prize-winning papers and their citation networks based on the American Physical Society(APS)and the Microsoft Academic Graph(MAG)dataset to test the accuracy of our proposed method(NCCAS).In addition,we use 654,148 articles published in the field of computer science from 2000 to 2009 in the MAG dataset to validate the distinguishability and robustness of NCCAS.Finding:Compared with the state-of-the-art methods,NCCAS gives the most accurate prediction of Nobel laureates.Furthermore,the leading author of the paper identified by NCCAS is more distinguishable compared with other co-authors.The results by NCCAS are also more robust to malicious manipulation.Finally,we perform ablation studies to show the contribution of different components in our methods.Research limitations:Due to limited ground truth on the true leading author of a work,the accuracy of NCCAS and other related methods can only be tested in Nobel Physics Prize-winning papers.Practical implications:NCCAS is successfully applied to a large number of publications,demonstrating its potential in analyzing the relationship between the contribution and the recognition of authors with different by-line orders.Originality/value:Compared with existing methods,NCCAS not only identifies the leading author of a paper more accurately,but also makes the deification more distinguishable and more robust,providing a new tool for related studies.
文摘As an important part of Chinese excellent traditional culture,Jingchu culture has profound historical and cultural value.The exploration and application of Jingchu culture elements is conducive to carrying forward and inheriting history and culture and enriching urban cultural deposits.Theme park is an important place to spread and promote Jingchu culture.It is of far-reaching significance to study and explore the integration of Jingchu culture elements into the construction of theme park.This paper analyzes the concept and connotation of Jingchu culture,combs and analyzes the extraction content of Jingchu culture elements,and summarizes the expression pathways of Jingchu culture elements in theme park and the practical application in Jingzhou Fantawild,in order to provide certain inspiration and reference for contemporary landscape designers to better inherit and develop Jingchu culture elements.
基金Scientific Research Innovation Project of China University of Political Science and Law Item(No.:21ZFY88001)The Fundamental Research Funds for the Central Universities。
文摘Sunshine Sports has played a significant role in promoting the teaching of Taekwondo in colleges and universities.By analyzing the connection between the Sunshine Sports concept and Taekwondo teaching,the characteristics of the Sunshine Sports concept in Taekwondo teaching in colleges and universities are discussed.This article expounds on the influencing factors in implementing the Sunshine Sports concept and discusses how to carry out scientific and reasonable teaching methods.
