Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and...Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and metastasis.However,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration.Fortunately,the further development of microfluidic technology has provided solutions for the study of such questions.We utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,respectively.By combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells.The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences observed.Furthermore,we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration side.However,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak.These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished.The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening.展开更多
Heat stress can restrict plant growth,development,and crop yield.As essential plant antioxidants,carotenoids play significant roles in plant stress resistance.b-carotene hydroxylase(BHY)and b-carotene ketolase(BKT),wh...Heat stress can restrict plant growth,development,and crop yield.As essential plant antioxidants,carotenoids play significant roles in plant stress resistance.b-carotene hydroxylase(BHY)and b-carotene ketolase(BKT),which catalyze the conversions of b-carotene to zeaxanthin and b-carotene to canthaxanthin,respectively,are key enzymes in the carotenoid biosynthetic pathway,but little is known about their potential functions in stress resistance.Here,we investigated the roles of b-carotene hydroxylase and b-carotene ketolase during heat stress in Physcomitrella patens through expressing a b-carotene ketolase gene from Chlamydomonas reinhardtii(Cr BKT)and a b-carotene hydroxylase gene from Haematococcus pluvialis(Hp BHY)in the moss P.patens.In transgenic moss expressing these genes,carotenoids content increased(especially lutein content),and heat stress tolerance increased,with reduced leafy tissue necrosis.To investigate the mechanism of this heat stress resistance,we measured various physiological indicators and found a lower malondialdehyde level,higher peroxidase and superoxide dismutase activities,and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress.These results demonstrate that Cr BKT and Hp BHY increase plant heat stress resistance through the antioxidant and damage repair metabolism,which is related to abscisic acid and salicylate signaling.展开更多
Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the abil...Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy metals.However,little is known about the genetic mechanisms of Mn tolerance in A.paniculata.In this study,we found that Mn tolerance and ability to accumulate Mn were higher in A.paniculata than in Arabidopsis thaliana.The mechanisms underlying the response and recovery of A.paniculata to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),respectively.In response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and lineage.Genes that were differentially expressed during Mn response and recovery belong to several dominant KEGG pathways.An early response to Mn toxicity in A.paniculata includes the upregulation of genes involved in glutathione metabolism.ATP-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn response.Genes in the phenylpropanoid pathway were differentially expressed in the repair process after Mn treatment.These findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.展开更多
BACKGROUND Pseudogout is a benign joint lesion caused by the deposition of calcium pyrophosphate dihydrate crystals,but it is invasive.Pseudogout of the temporomandibular joint(TMJ)is uncommon,and it rarely invades th...BACKGROUND Pseudogout is a benign joint lesion caused by the deposition of calcium pyrophosphate dihydrate crystals,but it is invasive.Pseudogout of the temporomandibular joint(TMJ)is uncommon,and it rarely invades the skull base or penetrates into the middle cranial fossa.The disease has no characteristic clinical manifestations and is easily misdiagnosed.CASE SUMMARY We present two cases of tophaceous pseudogout of the TMJ invading the middle cranial fossa.A 46-year-old woman with a history of diabetes for more than 10 years was admitted to the hospital due to swelling and pain in the right temporal region.Another patient,a 52-year-old man with a mass in the left TMJ for 6 years,was admitted to the hospital.Maxillofacial imaging showed a calcified mass and severe bone destruction of the skull base in the TMJ area.Both patients underwent excision of the lesion.The lesion was pathologically diagnosed as tophaceous pseudogout.The symptoms in these patients were relieved after surgery.CONCLUSION Tophaceous pseudogout should be considered when there is a calcified mass in the TMJ with or without bone destruction.A pathological examination is the gold standard for diagnosing this disease.Surgical treatment is currently the recommended treatment,and the prognosis is good after surgery.展开更多
Objective:To analyze and evaluate the clinical efficacy of different treatment options for patients with peri-implant infection.Methods:The study period was from June 2019 to June 2020.Seventy-six patients with peri-i...Objective:To analyze and evaluate the clinical efficacy of different treatment options for patients with peri-implant infection.Methods:The study period was from June 2019 to June 2020.Seventy-six patients with peri-implant infection that were treated in Stomatological Hospital of Hefei were selected as study samples.The patients were divided into a research group and a control group by random number table method in which the sample size of each group was n=38.Patients in the control group were treated with drugs combined with ultrasonic cleaning while patients in the research group were treated with guided bone regeneration combined with flap curettage.The indicators of the two groups were compared and analyzed.Results:The plaque index(PLI),probing depth(PD),and sulcus bleeding index(SBI)of the two groups after treatment were lower than those before treatment(P<0.05).There was no significant difference between the groups(P>0.05).However,the height of the implant marginal bone of the study group after treatment was higher than that of the control group(P<0.05)..Conclusion:The use of guided bone regeneration combined with flap curettage for patients with infection around dental implants promotes the fusion of bone and implants.The clinical effect was significant and this should be comprehensively promoted in medical institutions at all levels.展开更多
Spermatogenesis is a critical part of reproduction in insects;however,its molecular mechanism is still largely unknown.In this study,we identified a testis-specific gene CG3526 in Drosophila melanogaster.Bioinformatic...Spermatogenesis is a critical part of reproduction in insects;however,its molecular mechanism is still largely unknown.In this study,we identified a testis-specific gene CG3526 in Drosophila melanogaster.Bioinformatics analysis showed that CG3526 contains a zinc binding domain and 2 C2H2 type zinc fingers,and it is clustered to the vertebrate really interesting new gene(RING)family E3 ubiquitin-protein ligases.When CG3526 was knocked down by RNA interference(RNAi),the testis became much smaller in size,and the apical tip exhibited a sharp and thin end instead of the blunt and round shape in the control testis.More importantly,compared to the control flies,only a few mature sperm were present in the seminal vesicle of C587-Gal4>CG3526 RNAi flies.Immunofluorescence staining of the testis from CG3526 RNAi flies showed that the homeostasis of testis stem cell niche was disrupted,cell distribution in the apical tip was scattered,and the process of spermatogenesis was not completed.Furthermore,we found that the phenotype of CG3526 RNAi flies’testis was similar to that of testis of Stat92E RNAi flies,the expression level of CG3526 was significantly downregulated in the Stat92EF06346 mutant flies,and the promoter activity of CG3526 was upregulated by STAT92E.Taken together,our results indicated that CG3526 is a downstream effector gene in the JAK-STAT signaling pathway that plays a key role in the spermatogenesis of Drosophila.展开更多
The exploitation of a highly selective and sensitive probe to detect both cancer marker and metal ion is of great importance.In this work,the "one stone two bird" agent of 1,10-phenanthroline(phen) is design...The exploitation of a highly selective and sensitive probe to detect both cancer marker and metal ion is of great importance.In this work,the "one stone two bird" agent of 1,10-phenanthroline(phen) is designed to disrupt the polymeric lanthanide MOFs(LnMOFs,[Ln(CHO_(2))_(3)]n,Ln=Tb,la;Eu,1 b,CHO_(2)=formic acid) {[Ln(CHO_(2))_(4)·(C_(2) H_(8) N)]n,Ln=Y,2 a;Gd,2 b;Dy,2 c,C_(2)H_(8) N=dimethylamine}) into a soluble mononuclear species [Ln(phen)_(2)(NO_(3))_(3),Ln=Tb,3 a;Eu,3 b] as well as to provide an antenna for efficient photons absorption,resulting in an ultra-high luminescence quantum yield(QY,90%) europium complex.The luminescence QY is among the highest record of monomeric(zero-dimensional) lanthanide complexes.Furthermore,mononuclear Tb3+complex(3 a) functions as a multiplex sensor towards both Fe^(2+)and cancer marker of 5-hydroxyindole-3-acetic acid(5-HIAA).Importantly,the limit of detection(LOD)for sensing 5-HIAA is an ultra-sensitive value of 1 × 10 s mol/L,which is even lower than that necessary for the early diagnosis of carcinoid tumors.More interestingly,sensing results in simulated urine reveals that 3 a has potential application for early diagnosis in the clinic.展开更多
L-kynurenine(L-kyn)is a marker of prostate cancer.At present,the expensive instruments are usually applied to detect L-kyn clinically,which limits its wide application for cancer diagnosis.Herein,three lanthanide meta...L-kynurenine(L-kyn)is a marker of prostate cancer.At present,the expensive instruments are usually applied to detect L-kyn clinically,which limits its wide application for cancer diagnosis.Herein,three lanthanide metal-organic frameworks([Ln(CHO_(2))_(3)]_(n),Ln=Eu,Gd,and Tb)were designed and obtained,and detailly characterized by single crystal X-ray diffraction(SCXRD),powder X-ray diffraction(PXRD),Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TGA),and luminescence spectroscopy.Further study reveals that[Tb(CHO_(2))_(3)]_(n)is a highly selective,ultra-sensitive,of strong anti-interference,highly stable,and non-expensive sensor for prostate cancer marker L-kyn.The limit of detection(LOD)for L-kyn sensing is a highly sensitive value of 1.0×10^(−9)mol/L.Furthermore,the sensing mechanism is discussed in detail.展开更多
Shortcut nitrification-denitrification,anaerobic ammonium oxidation(ANAMMOX),and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter(EGSB-BAF)integrated system.As...Shortcut nitrification-denitrification,anaerobic ammonium oxidation(ANAMMOX),and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter(EGSB-BAF)integrated system.As fed different synthetic wastewater with chemical oxygen demand(COD)of 300-1200 mg·L^(-1)and NH_(4)^(+)-N of 30-120 mg·L^(-1)at the outer recycle ratio of 200%,the influence of influent on ANAMMOX in the integrated system was investigated in this paper.The experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX;however,when an influent with the low concentration of COD was used,the opposite changes could be observed.Higher influent NH_(4)^(+)-N concentration favored ANAMMOX when the COD concentration of influent was fixed.Therefore,low COD=NH_(4)^(+)-N ratio would decrease competition for nitrite between ANAMMOX and denitrification,which was favorable for reducing the negative effect of organic COD on ANAMMOX.The good performance of the integrated system indicated that the bacterial community of denitrification,ANAMMOX,and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.展开更多
Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in plan...Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in plants.However,their roles in insects upon entomopathogen infection are unknown.In this study,54 bHLH genes in 41 families were identified in a polyphagous pest,Spodoptera litura,including a new bHLH gene in group B,which is specifically present in Lepidoptera and was thus named Lep.The conserved amino acids in the bHLH domain,structural architecture,and chromosomal distribution of bHLH genes in S.litura were analyzed.The bHLH genes in Plutella xylostella and Apis mellifera were also updated,and genome-wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were performed.The expression profiles of S.litura bHLH(SlbHLH)genes in 3 tissues at different developmental stages and their responses to S.litura nucleopolyhedrovirus(SpltNPV),Nomuraea rileyi(Nr),and Bacillus thuringiensis(Bt)infection were investigated.More SlbHLHs in group B were expressed and differentially expressed during pathogen infections,and SlbHLHs tended to be downregulated in the midgut of S.litura larvae after B.thuringiensis treatment.Our study provides an overview of bHLH family members in S.litura and their responses to different pathogens used for pest biocontrol.These findings on bHLH members may contribute to uncovering the mechanism of host-pathogen interaction.展开更多
Flow regime is a key driver of invasive aquatic organisms,and the invasiveness of mangrove species may be simultaneously attributed to plant traits and flowing hydrological conditions at the estuary scale.We focused o...Flow regime is a key driver of invasive aquatic organisms,and the invasiveness of mangrove species may be simultaneously attributed to plant traits and flowing hydrological conditions at the estuary scale.We focused on hydrological and topographic conditions for a non-native mangrove species,Sonneratia apetala,in Zhangjiang Estuary of Fujian,China.A hydrological model and a micro-topographic model were used to predict its dispersal and early establishment,and field surveys and simulated experiments were integrated to estimate its future dispersal patterns.The mesohaline mudflat with a salinity of 8~10 PSU at the mangrove seaward edge was the most likely colonization area for S.apetala under current conditions.The south-western region of the estuary with native mangroves was the most likely area for its colonization according to the unstructured-grid finite-volume community ocean model(FVCOM)in September,when the largest tidal currents within a year and the maximum fruit maturity period occur.Approximately 42%of the mudflats throughout the whole estuary may be available for seedling establishment under the future sea-level rise RCP 4.5 scenarios compared with 44%for current establishment;however,the RCP 8.5 scenarios would significantly decrease seedling establishment by 2100 due to serious tidal inundation according to the micro-topographical model.展开更多
Aim:Ear reconstruction is a challenge for plastic and reconstructive surgeons.The ear requires sufficient skin coverage and a three-dimensional(3D)cartilage framework.In this paper,the authors present their 10-year ex...Aim:Ear reconstruction is a challenge for plastic and reconstructive surgeons.The ear requires sufficient skin coverage and a three-dimensional(3D)cartilage framework.In this paper,the authors present their 10-year experience in microtia reconstruction using tissue expansion and an autogenous rib cartilage framework.Methods:Ear reconstruction was performed in 3 operative stages.During the first procedure,a 50-80 mL kidney or cylinder-shaped expander was implanted deep to the subcutaneous fascia of the retroauricular mastoid region.Over a period of 3-5 months,the expander was filled to a final volume of 80-110 mL.In the next operation,the retroauricular fascia was eliminated or reserved following expander removal,and the autogenous costal cartilage framework was placed below the expanded skin flap.At the third and final stage,the earlobe transposition,tragus construction and conchal deepening were performed.Results:A total of 165 patients(166 ears)were reconstructed using tissue expansion and an autogenous rib cartilage framework.Complications included hematomas in 3 cases,expander exposure in 8 cases,cartilage exposure in 6 cases,infection and cartilage resorption in 2 cases,exposure of steel wire in 4 cases,and aseptic seroma in 2 cases.Follow-up ranging from 3 months to 5 years showed that 159 patients were satisfied with the reconstructed ear including size,location,projection,convolution,skin-colour matching,symmetry with opposite ear.Conclusion:Expansion of the retroauricular skin and fascia can provide sufficient non-hair-bearing skin and tissue for coverage of a three-dimensional costal cartilage framework.Avoidance and prompt treatment of complications are advised in order to obtain a satisfactory reconstruction of the ear.展开更多
基金support from the National Natural Science Foundation of China(Grant Nos.11974066,12174041,12104134,T2350007,and 12347178)the Fundamental and Advanced Research Program of Chongqing(Grant No.cstc2019jcyj-msxm X0477)+3 种基金the Natural Science Foundation of Chongqing(Grant No.CSTB2022NSCQMSX1260)the Science and Technology Research Program of Chongqing Municipal Education Commission(Grant No.KJQN202301333)the Scientific Research Fund of Chongqing University of Arts and Sciences(Grant Nos.R2023HH03 and P2022HH05)College Students’Innovation and Entrepreneurship Training Program of Chongqing Municipal(Grant No.S202310642002)。
文摘Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and metastasis.However,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration.Fortunately,the further development of microfluidic technology has provided solutions for the study of such questions.We utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,respectively.By combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells.The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences observed.Furthermore,we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration side.However,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak.These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished.The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening.
基金supported by the CAS Pioneer Hundred Talents Programthe National Natural Science Foundation of China (31571262)Yunnan Natural Science Foundation (2017FB031)
文摘Heat stress can restrict plant growth,development,and crop yield.As essential plant antioxidants,carotenoids play significant roles in plant stress resistance.b-carotene hydroxylase(BHY)and b-carotene ketolase(BKT),which catalyze the conversions of b-carotene to zeaxanthin and b-carotene to canthaxanthin,respectively,are key enzymes in the carotenoid biosynthetic pathway,but little is known about their potential functions in stress resistance.Here,we investigated the roles of b-carotene hydroxylase and b-carotene ketolase during heat stress in Physcomitrella patens through expressing a b-carotene ketolase gene from Chlamydomonas reinhardtii(Cr BKT)and a b-carotene hydroxylase gene from Haematococcus pluvialis(Hp BHY)in the moss P.patens.In transgenic moss expressing these genes,carotenoids content increased(especially lutein content),and heat stress tolerance increased,with reduced leafy tissue necrosis.To investigate the mechanism of this heat stress resistance,we measured various physiological indicators and found a lower malondialdehyde level,higher peroxidase and superoxide dismutase activities,and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress.These results demonstrate that Cr BKT and Hp BHY increase plant heat stress resistance through the antioxidant and damage repair metabolism,which is related to abscisic acid and salicylate signaling.
基金This research was supported by Natural Science Foundation of Hunan Province,China(2020JJ4293)Scientific Research Project of the Hunan Education Department,China(18B215).
文摘Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy metals.However,little is known about the genetic mechanisms of Mn tolerance in A.paniculata.In this study,we found that Mn tolerance and ability to accumulate Mn were higher in A.paniculata than in Arabidopsis thaliana.The mechanisms underlying the response and recovery of A.paniculata to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),respectively.In response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and lineage.Genes that were differentially expressed during Mn response and recovery belong to several dominant KEGG pathways.An early response to Mn toxicity in A.paniculata includes the upregulation of genes involved in glutathione metabolism.ATP-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn response.Genes in the phenylpropanoid pathway were differentially expressed in the repair process after Mn treatment.These findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.
文摘BACKGROUND Pseudogout is a benign joint lesion caused by the deposition of calcium pyrophosphate dihydrate crystals,but it is invasive.Pseudogout of the temporomandibular joint(TMJ)is uncommon,and it rarely invades the skull base or penetrates into the middle cranial fossa.The disease has no characteristic clinical manifestations and is easily misdiagnosed.CASE SUMMARY We present two cases of tophaceous pseudogout of the TMJ invading the middle cranial fossa.A 46-year-old woman with a history of diabetes for more than 10 years was admitted to the hospital due to swelling and pain in the right temporal region.Another patient,a 52-year-old man with a mass in the left TMJ for 6 years,was admitted to the hospital.Maxillofacial imaging showed a calcified mass and severe bone destruction of the skull base in the TMJ area.Both patients underwent excision of the lesion.The lesion was pathologically diagnosed as tophaceous pseudogout.The symptoms in these patients were relieved after surgery.CONCLUSION Tophaceous pseudogout should be considered when there is a calcified mass in the TMJ with or without bone destruction.A pathological examination is the gold standard for diagnosing this disease.Surgical treatment is currently the recommended treatment,and the prognosis is good after surgery.
基金a project titled "Effect of Systematic Health Education on Prevention of Peri-implant Diseases"。
文摘Objective:To analyze and evaluate the clinical efficacy of different treatment options for patients with peri-implant infection.Methods:The study period was from June 2019 to June 2020.Seventy-six patients with peri-implant infection that were treated in Stomatological Hospital of Hefei were selected as study samples.The patients were divided into a research group and a control group by random number table method in which the sample size of each group was n=38.Patients in the control group were treated with drugs combined with ultrasonic cleaning while patients in the research group were treated with guided bone regeneration combined with flap curettage.The indicators of the two groups were compared and analyzed.Results:The plaque index(PLI),probing depth(PD),and sulcus bleeding index(SBI)of the two groups after treatment were lower than those before treatment(P<0.05).There was no significant difference between the groups(P>0.05).However,the height of the implant marginal bone of the study group after treatment was higher than that of the control group(P<0.05)..Conclusion:The use of guided bone regeneration combined with flap curettage for patients with infection around dental implants promotes the fusion of bone and implants.The clinical effect was significant and this should be comprehensively promoted in medical institutions at all levels.
基金This work was supported by the National Natural Science Foundation of China(No.31970474 and No.32272606)China Postdoctoral Science Foundation(No.2022M721219).
文摘Spermatogenesis is a critical part of reproduction in insects;however,its molecular mechanism is still largely unknown.In this study,we identified a testis-specific gene CG3526 in Drosophila melanogaster.Bioinformatics analysis showed that CG3526 contains a zinc binding domain and 2 C2H2 type zinc fingers,and it is clustered to the vertebrate really interesting new gene(RING)family E3 ubiquitin-protein ligases.When CG3526 was knocked down by RNA interference(RNAi),the testis became much smaller in size,and the apical tip exhibited a sharp and thin end instead of the blunt and round shape in the control testis.More importantly,compared to the control flies,only a few mature sperm were present in the seminal vesicle of C587-Gal4>CG3526 RNAi flies.Immunofluorescence staining of the testis from CG3526 RNAi flies showed that the homeostasis of testis stem cell niche was disrupted,cell distribution in the apical tip was scattered,and the process of spermatogenesis was not completed.Furthermore,we found that the phenotype of CG3526 RNAi flies’testis was similar to that of testis of Stat92E RNAi flies,the expression level of CG3526 was significantly downregulated in the Stat92EF06346 mutant flies,and the promoter activity of CG3526 was upregulated by STAT92E.Taken together,our results indicated that CG3526 is a downstream effector gene in the JAK-STAT signaling pathway that plays a key role in the spermatogenesis of Drosophila.
基金supported by the National Natural Science Foundation of China (51962008)。
文摘The exploitation of a highly selective and sensitive probe to detect both cancer marker and metal ion is of great importance.In this work,the "one stone two bird" agent of 1,10-phenanthroline(phen) is designed to disrupt the polymeric lanthanide MOFs(LnMOFs,[Ln(CHO_(2))_(3)]n,Ln=Tb,la;Eu,1 b,CHO_(2)=formic acid) {[Ln(CHO_(2))_(4)·(C_(2) H_(8) N)]n,Ln=Y,2 a;Gd,2 b;Dy,2 c,C_(2)H_(8) N=dimethylamine}) into a soluble mononuclear species [Ln(phen)_(2)(NO_(3))_(3),Ln=Tb,3 a;Eu,3 b] as well as to provide an antenna for efficient photons absorption,resulting in an ultra-high luminescence quantum yield(QY,90%) europium complex.The luminescence QY is among the highest record of monomeric(zero-dimensional) lanthanide complexes.Furthermore,mononuclear Tb3+complex(3 a) functions as a multiplex sensor towards both Fe^(2+)and cancer marker of 5-hydroxyindole-3-acetic acid(5-HIAA).Importantly,the limit of detection(LOD)for sensing 5-HIAA is an ultra-sensitive value of 1 × 10 s mol/L,which is even lower than that necessary for the early diagnosis of carcinoid tumors.More interestingly,sensing results in simulated urine reveals that 3 a has potential application for early diagnosis in the clinic.
基金Project supported by the National Natural Science Foundation of China(51962008)Jiangxi Provincial Natural Science Foundation(20202BABL203018)。
文摘L-kynurenine(L-kyn)is a marker of prostate cancer.At present,the expensive instruments are usually applied to detect L-kyn clinically,which limits its wide application for cancer diagnosis.Herein,three lanthanide metal-organic frameworks([Ln(CHO_(2))_(3)]_(n),Ln=Eu,Gd,and Tb)were designed and obtained,and detailly characterized by single crystal X-ray diffraction(SCXRD),powder X-ray diffraction(PXRD),Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TGA),and luminescence spectroscopy.Further study reveals that[Tb(CHO_(2))_(3)]_(n)is a highly selective,ultra-sensitive,of strong anti-interference,highly stable,and non-expensive sensor for prostate cancer marker L-kyn.The limit of detection(LOD)for L-kyn sensing is a highly sensitive value of 1.0×10^(−9)mol/L.Furthermore,the sensing mechanism is discussed in detail.
基金This research was supported by the Natural Science Foundation of China(Grant No.50378094).
文摘Shortcut nitrification-denitrification,anaerobic ammonium oxidation(ANAMMOX),and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter(EGSB-BAF)integrated system.As fed different synthetic wastewater with chemical oxygen demand(COD)of 300-1200 mg·L^(-1)and NH_(4)^(+)-N of 30-120 mg·L^(-1)at the outer recycle ratio of 200%,the influence of influent on ANAMMOX in the integrated system was investigated in this paper.The experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX;however,when an influent with the low concentration of COD was used,the opposite changes could be observed.Higher influent NH_(4)^(+)-N concentration favored ANAMMOX when the COD concentration of influent was fixed.Therefore,low COD=NH_(4)^(+)-N ratio would decrease competition for nitrite between ANAMMOX and denitrification,which was favorable for reducing the negative effect of organic COD on ANAMMOX.The good performance of the integrated system indicated that the bacterial community of denitrification,ANAMMOX,and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.
基金This research was funded by grants from the National Key R&D Program of China(No,2019YFD1002100)National Natural Science Foundation of China(No.31901941 and No.31970474)+1 种基金Natural Science Foun-dation of Guangdong Province(No.202102020966)China Postdoctoral Science Foundation(No.2020M682752).The authors are grateful for the generous gift of S.litura nucleopolyhedrovirus(SpItNPV)from Professor Kai Yang at Sun Yat-Sen University,Nomuruea rileyi(Nr06)from Professor Xusheng Liu at Central China Normal University,and Bacillus thuringiensis(WB9)from Professor Xiong Guan at Fujian Agriculture and Forestry University.
文摘Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in plants.However,their roles in insects upon entomopathogen infection are unknown.In this study,54 bHLH genes in 41 families were identified in a polyphagous pest,Spodoptera litura,including a new bHLH gene in group B,which is specifically present in Lepidoptera and was thus named Lep.The conserved amino acids in the bHLH domain,structural architecture,and chromosomal distribution of bHLH genes in S.litura were analyzed.The bHLH genes in Plutella xylostella and Apis mellifera were also updated,and genome-wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were performed.The expression profiles of S.litura bHLH(SlbHLH)genes in 3 tissues at different developmental stages and their responses to S.litura nucleopolyhedrovirus(SpltNPV),Nomuraea rileyi(Nr),and Bacillus thuringiensis(Bt)infection were investigated.More SlbHLHs in group B were expressed and differentially expressed during pathogen infections,and SlbHLHs tended to be downregulated in the midgut of S.litura larvae after B.thuringiensis treatment.Our study provides an overview of bHLH family members in S.litura and their responses to different pathogens used for pest biocontrol.These findings on bHLH members may contribute to uncovering the mechanism of host-pathogen interaction.
基金This work was supported by the National Key Research and Development Program of China[2017YFC0506103]National Natural Science Foundation of China[31770579]+2 种基金the Fundamental Research Funds for the Central Universities of China(20720180118)the Key Laboratory of the Coastal and Wetland Ecosystems(WELRI201601)the State Key Laboratory of Marine Environmental Science(MELRI1603).
文摘Flow regime is a key driver of invasive aquatic organisms,and the invasiveness of mangrove species may be simultaneously attributed to plant traits and flowing hydrological conditions at the estuary scale.We focused on hydrological and topographic conditions for a non-native mangrove species,Sonneratia apetala,in Zhangjiang Estuary of Fujian,China.A hydrological model and a micro-topographic model were used to predict its dispersal and early establishment,and field surveys and simulated experiments were integrated to estimate its future dispersal patterns.The mesohaline mudflat with a salinity of 8~10 PSU at the mangrove seaward edge was the most likely colonization area for S.apetala under current conditions.The south-western region of the estuary with native mangroves was the most likely area for its colonization according to the unstructured-grid finite-volume community ocean model(FVCOM)in September,when the largest tidal currents within a year and the maximum fruit maturity period occur.Approximately 42%of the mudflats throughout the whole estuary may be available for seedling establishment under the future sea-level rise RCP 4.5 scenarios compared with 44%for current establishment;however,the RCP 8.5 scenarios would significantly decrease seedling establishment by 2100 due to serious tidal inundation according to the micro-topographical model.
基金supported by the grants of National Natural Science Foundation of China(No.81660319)Health Science and Technology Plan Projects of Yunnan province(No.2016NS266).
文摘Aim:Ear reconstruction is a challenge for plastic and reconstructive surgeons.The ear requires sufficient skin coverage and a three-dimensional(3D)cartilage framework.In this paper,the authors present their 10-year experience in microtia reconstruction using tissue expansion and an autogenous rib cartilage framework.Methods:Ear reconstruction was performed in 3 operative stages.During the first procedure,a 50-80 mL kidney or cylinder-shaped expander was implanted deep to the subcutaneous fascia of the retroauricular mastoid region.Over a period of 3-5 months,the expander was filled to a final volume of 80-110 mL.In the next operation,the retroauricular fascia was eliminated or reserved following expander removal,and the autogenous costal cartilage framework was placed below the expanded skin flap.At the third and final stage,the earlobe transposition,tragus construction and conchal deepening were performed.Results:A total of 165 patients(166 ears)were reconstructed using tissue expansion and an autogenous rib cartilage framework.Complications included hematomas in 3 cases,expander exposure in 8 cases,cartilage exposure in 6 cases,infection and cartilage resorption in 2 cases,exposure of steel wire in 4 cases,and aseptic seroma in 2 cases.Follow-up ranging from 3 months to 5 years showed that 159 patients were satisfied with the reconstructed ear including size,location,projection,convolution,skin-colour matching,symmetry with opposite ear.Conclusion:Expansion of the retroauricular skin and fascia can provide sufficient non-hair-bearing skin and tissue for coverage of a three-dimensional costal cartilage framework.Avoidance and prompt treatment of complications are advised in order to obtain a satisfactory reconstruction of the ear.
