Biosensors have been developed using various types of sensing elements like biomacromolecules (viz. enzymes, antibodies, receptors, nucleic acids, etc.) organelles, tissues, intact cells of both microorganisms and hig...Biosensors have been developed using various types of sensing elements like biomacromolecules (viz. enzymes, antibodies, receptors, nucleic acids, etc.) organelles, tissues, intact cells of both microorganisms and higher organisms. A recent trend is the emergence of aptamers as sensing elements that has the potential to replace all the above ligands. This is possible due to the unique features of aptamers (sensitivity, specificity, reusability, stability, non-immunogenic- ity), which can be easily exploited in biosensor technology. Aptasensors are thus basically biosensors based on aptamers as ligand molecules. Here we review the various applications of aptasensors in health (specifically in diagnostics), food industry and environmental monitoring.展开更多
We report here the application of a medicinally important plant Amaranthus spinosus for the synthesis of gold nanoparticles (AuNPs). Different concentrations of ethanolic leaf extract of the plant were reacted with aq...We report here the application of a medicinally important plant Amaranthus spinosus for the synthesis of gold nanoparticles (AuNPs). Different concentrations of ethanolic leaf extract of the plant were reacted with aqueous solution of HAuCl4·4H2O under mild reaction conditions. Synthesis of AuNPs was confirmed from the UV-Vis study of surface plasmon resonance property of the colloidal solution. Transmission electron microscopy (TEM) revealed particles as spherical and triangular in shape. X-ray diffraction (XRD) confirmed the crystalline nature of AuNPs with average size of 10.74 nm as determined by Debye-Scherrer’s Equation. Fourier transform infra-red (FT-IR) analysis of leaf extract and lyophilized AuNPs showed the presence of various functional groups present in diverse phytochemicals. Energy dispersive X-ray (EDX) of purified AuNPs confirmed the formation of AuNPs and surface adsorption of biomolecules. We further investigated the toxicity of the synthesized AuNPs and found non toxic to the cancer cell lines and could be used for biomedical applications.展开更多
This report describes the use of ethnolic extract of Fagopyrum esculentum leaves for the synthesis of gold nanoparticles. UV-visible spectroscopy analysis indicated the successful formation of gold nanoparticles. The ...This report describes the use of ethnolic extract of Fagopyrum esculentum leaves for the synthesis of gold nanoparticles. UV-visible spectroscopy analysis indicated the successful formation of gold nanoparticles. The synthesized nanoparticles were characterized by transmission electron microscopy （TEM）, high resolution TEM （HRTEM） and were found to be spherical, hexagonal and triangular in shape with an average size of 8.3 nm. The crystalline nature of the gold nanoparticles was confirmed from X.ray diffraction （XRD） and selected-area electron diffraction （SAED） patterns. Fourier transform infrared （FT-IR） and energy.dispersive X-ray analysis （EDX） suggested the presence of organic biomolecules on the surface of the gold nanoparticles. Cytotoxicity tests against human HeLa, MCF-7 and IMR-32 cancer cell lines revealed that the gold nanoparticles were non-toxic and thus have potential for use in various biomedical applications.展开更多
Gold nanoparticles （AuNPs） were synthesized by sonication using ethanolic leaf extract of Andrographis paniculata. We investigated the optimum parameters for AuNP synthesis and functionalization with polycaprolacton...Gold nanoparticles （AuNPs） were synthesized by sonication using ethanolic leaf extract of Andrographis paniculata. We investigated the optimum parameters for AuNP synthesis and functionalization with polycaprolactone-gelatin （PCL-GL） composites. The AuNPs were characterized with various biophysical techniques such as TEM, XRD, FT-IR and EDX spectroscopy. TEM images showed that nanoparticles were spherical in shape with a size range from 5 to 75 nm. EDX analysis revealed the presence of molecular oxygen and carbon on the surface of AuNPs. The synthesized AuNPs were tested for their effect on HeLa （human cervical cancer） and MCF- 7 （human breast cancer） cell lines and found to be nontoxic and biocompatible, which are potential carriers for hydrophobic drugs.展开更多
文摘Biosensors have been developed using various types of sensing elements like biomacromolecules (viz. enzymes, antibodies, receptors, nucleic acids, etc.) organelles, tissues, intact cells of both microorganisms and higher organisms. A recent trend is the emergence of aptamers as sensing elements that has the potential to replace all the above ligands. This is possible due to the unique features of aptamers (sensitivity, specificity, reusability, stability, non-immunogenic- ity), which can be easily exploited in biosensor technology. Aptasensors are thus basically biosensors based on aptamers as ligand molecules. Here we review the various applications of aptasensors in health (specifically in diagnostics), food industry and environmental monitoring.
文摘We report here the application of a medicinally important plant Amaranthus spinosus for the synthesis of gold nanoparticles (AuNPs). Different concentrations of ethanolic leaf extract of the plant were reacted with aqueous solution of HAuCl4·4H2O under mild reaction conditions. Synthesis of AuNPs was confirmed from the UV-Vis study of surface plasmon resonance property of the colloidal solution. Transmission electron microscopy (TEM) revealed particles as spherical and triangular in shape. X-ray diffraction (XRD) confirmed the crystalline nature of AuNPs with average size of 10.74 nm as determined by Debye-Scherrer’s Equation. Fourier transform infra-red (FT-IR) analysis of leaf extract and lyophilized AuNPs showed the presence of various functional groups present in diverse phytochemicals. Energy dispersive X-ray (EDX) of purified AuNPs confirmed the formation of AuNPs and surface adsorption of biomolecules. We further investigated the toxicity of the synthesized AuNPs and found non toxic to the cancer cell lines and could be used for biomedical applications.
文摘This report describes the use of ethnolic extract of Fagopyrum esculentum leaves for the synthesis of gold nanoparticles. UV-visible spectroscopy analysis indicated the successful formation of gold nanoparticles. The synthesized nanoparticles were characterized by transmission electron microscopy （TEM）, high resolution TEM （HRTEM） and were found to be spherical, hexagonal and triangular in shape with an average size of 8.3 nm. The crystalline nature of the gold nanoparticles was confirmed from X.ray diffraction （XRD） and selected-area electron diffraction （SAED） patterns. Fourier transform infrared （FT-IR） and energy.dispersive X-ray analysis （EDX） suggested the presence of organic biomolecules on the surface of the gold nanoparticles. Cytotoxicity tests against human HeLa, MCF-7 and IMR-32 cancer cell lines revealed that the gold nanoparticles were non-toxic and thus have potential for use in various biomedical applications.
文摘Gold nanoparticles （AuNPs） were synthesized by sonication using ethanolic leaf extract of Andrographis paniculata. We investigated the optimum parameters for AuNP synthesis and functionalization with polycaprolactone-gelatin （PCL-GL） composites. The AuNPs were characterized with various biophysical techniques such as TEM, XRD, FT-IR and EDX spectroscopy. TEM images showed that nanoparticles were spherical in shape with a size range from 5 to 75 nm. EDX analysis revealed the presence of molecular oxygen and carbon on the surface of AuNPs. The synthesized AuNPs were tested for their effect on HeLa （human cervical cancer） and MCF- 7 （human breast cancer） cell lines and found to be nontoxic and biocompatible, which are potential carriers for hydrophobic drugs.
