The tomato(Solanum lycopersicum L.)is one of the world’s most important vegetable crops.Still,phytopathogenic bacteria affect the yield and quality of tomato cultivation,like Agrobacterium tumefeciens(At),Clavibacter...The tomato(Solanum lycopersicum L.)is one of the world’s most important vegetable crops.Still,phytopathogenic bacteria affect the yield and quality of tomato cultivation,like Agrobacterium tumefeciens(At),Clavibacter michiganensis subsp.michiganensis(Cmm),Pseudomonas syringae pv.tomato(Pst),Ralstonia solanacearum(Rs),and Xanthomonas axonopodis(Xa).Synthetic chemical products are used mostly on disease plant control,but overuse generates resistance to bacterial control.This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam.leaves against At,Cmm,Pst,Rs,and Xa,as well as information about this plant species’chemical composition.Antibacterial activity against pathogens observed by microplate technique,phytochemical screening,and FTIR analysis revealed different bio-active compounds on ethanolic extracts with antibacterial activity.The growth inhibition rate ranged between 0.08%and 99.94%.The inhibitory concentration,IC50,required to inhibit 50%of At,Cmm,Pst,Rs,and Xa bacterial growth,was 276.67,350.48,277.85,351.49,and 283.22 mg/L,respectively.Inhibition of phytopathogen bacteria’s growth increased as the concentrations of the extract also increased.Moringa oleifera extract can be recommended as a potent bio-bactericide.展开更多
基金This work was supported by PRODEP throughthe support of the postdoctoral fellowship granted to the first author(Trade No.511-6/18-391)The authors thank the academic body of Ecología Aplicada(UAT-CA-156)for the support obtained from their researchers to conductthis investigation.
文摘The tomato(Solanum lycopersicum L.)is one of the world’s most important vegetable crops.Still,phytopathogenic bacteria affect the yield and quality of tomato cultivation,like Agrobacterium tumefeciens(At),Clavibacter michiganensis subsp.michiganensis(Cmm),Pseudomonas syringae pv.tomato(Pst),Ralstonia solanacearum(Rs),and Xanthomonas axonopodis(Xa).Synthetic chemical products are used mostly on disease plant control,but overuse generates resistance to bacterial control.This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam.leaves against At,Cmm,Pst,Rs,and Xa,as well as information about this plant species’chemical composition.Antibacterial activity against pathogens observed by microplate technique,phytochemical screening,and FTIR analysis revealed different bio-active compounds on ethanolic extracts with antibacterial activity.The growth inhibition rate ranged between 0.08%and 99.94%.The inhibitory concentration,IC50,required to inhibit 50%of At,Cmm,Pst,Rs,and Xa bacterial growth,was 276.67,350.48,277.85,351.49,and 283.22 mg/L,respectively.Inhibition of phytopathogen bacteria’s growth increased as the concentrations of the extract also increased.Moringa oleifera extract can be recommended as a potent bio-bactericide.
