Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collect...Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.展开更多
目的:比较富血小板血浆(platelet rich plasma,PRP)与体外冲击波治疗慢性跟腱止点腱病的临床疗效。方法:2019年2月至2021年8月,根据病例选择最终纳入42例跟腱止点腱病患者,分为PRP组(20例28足)与冲击波组(22例29足)。PRP组男12例,女8例...目的:比较富血小板血浆(platelet rich plasma,PRP)与体外冲击波治疗慢性跟腱止点腱病的临床疗效。方法:2019年2月至2021年8月,根据病例选择最终纳入42例跟腱止点腱病患者,分为PRP组(20例28足)与冲击波组(22例29足)。PRP组男12例,女8例;年龄47.00(28.00,50.75)岁;病程7.00(6.00,7.00)个月;予患侧跟腱止点区域进行富血小板血浆注射治疗。冲击波组男16例,女6例;年龄42.00(35.75,47.25)岁;病程7.00(6.00,8.00)个月;予患侧跟腱止点区及小腿三头肌区域进行冲击波治疗。分别于治疗前及治疗后1、3、6个月采用疼痛视觉模拟评分(visual analogue scale,VAS)及维多利亚体育研究中心跟腱问卷表(Victorian Institute of Sport Assessment-Achilles,VISAA)评分进行疗效评价,并对所有患者进行满意度调查。结果:两组治疗1、3、6个月后VAS及VISA-A评分均较治疗前改善(P<0.05);PRP组治疗6个月后VAS及VISA-A评分均优于治疗后1、3个月(P<0.05);冲击波组治疗6个月后VAS及VISA-A评分均差于治疗后1、3个月(P<0.05);两组治疗前、治疗后1、3个月VAS及VISA-A评分比较差异无统计学意义(P>0.05);PRP组治疗后6个月VAS及VISA-A评分优于冲击波组(P<0.05),PRP组满意度调查优于冲击波组(P<0.05)。结论:PRP注射对于慢性跟腱止点腱病具有良好的临床疗效,患者满意度高,且PRP注射治疗慢性跟腱止点腱病的中长期疗效更优于体外发散式冲击波。展开更多
文摘Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.
文摘目的:比较富血小板血浆(platelet rich plasma,PRP)与体外冲击波治疗慢性跟腱止点腱病的临床疗效。方法:2019年2月至2021年8月,根据病例选择最终纳入42例跟腱止点腱病患者,分为PRP组(20例28足)与冲击波组(22例29足)。PRP组男12例,女8例;年龄47.00(28.00,50.75)岁;病程7.00(6.00,7.00)个月;予患侧跟腱止点区域进行富血小板血浆注射治疗。冲击波组男16例,女6例;年龄42.00(35.75,47.25)岁;病程7.00(6.00,8.00)个月;予患侧跟腱止点区及小腿三头肌区域进行冲击波治疗。分别于治疗前及治疗后1、3、6个月采用疼痛视觉模拟评分(visual analogue scale,VAS)及维多利亚体育研究中心跟腱问卷表(Victorian Institute of Sport Assessment-Achilles,VISAA)评分进行疗效评价,并对所有患者进行满意度调查。结果:两组治疗1、3、6个月后VAS及VISA-A评分均较治疗前改善(P<0.05);PRP组治疗6个月后VAS及VISA-A评分均优于治疗后1、3个月(P<0.05);冲击波组治疗6个月后VAS及VISA-A评分均差于治疗后1、3个月(P<0.05);两组治疗前、治疗后1、3个月VAS及VISA-A评分比较差异无统计学意义(P>0.05);PRP组治疗后6个月VAS及VISA-A评分优于冲击波组(P<0.05),PRP组满意度调查优于冲击波组(P<0.05)。结论:PRP注射对于慢性跟腱止点腱病具有良好的临床疗效,患者满意度高,且PRP注射治疗慢性跟腱止点腱病的中长期疗效更优于体外发散式冲击波。