Porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infecte...Porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infected ones. In present study, a recombinant plasmid pET28a/NS1 was constructed by cloning the coding sequence for NS1 of PPV into pET28a, a bacterial expression vector. The NS1 protein was expressed in E. coli BL21(DE3) after induced by IPTG and the recombinant fusion protein was purified with affinity chromatography. Expression amount of NS1 protein was improved by optimizing the inducing parameters. The recombinant NS1 protein is reactive to PPV positive sera in Western blot and ELISA test and therefore can be applicable in differential diagnosis of PPV infections.展开更多
2022年美国胸科医师学会(American College of Chest Physicians,ACCP)发布抗栓治疗的围手术期管理指南。该指南主要针对4种临床情况提出抗栓建议,分别是接受维生素K拮抗剂、肝素(heparin)桥接、直接口服抗凝药物及抗血小板药物这4类药...2022年美国胸科医师学会(American College of Chest Physicians,ACCP)发布抗栓治疗的围手术期管理指南。该指南主要针对4种临床情况提出抗栓建议,分别是接受维生素K拮抗剂、肝素(heparin)桥接、直接口服抗凝药物及抗血小板药物这4类药物需要手术或侵入性操作患者以及围手术期实验室检测提供指导建议。该文就ACCP围手术期抗栓治疗既往版本指南进行比较,同时结合国内发布的《抗栓治疗病人接受非心脏手术围手术期管理上海专家共识(2021版)》和《中国血栓性疾病防治指南》对该指南进行全面解读,以期为临床围手术期应用抗血栓药物提供参考。展开更多
文摘Porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infected ones. In present study, a recombinant plasmid pET28a/NS1 was constructed by cloning the coding sequence for NS1 of PPV into pET28a, a bacterial expression vector. The NS1 protein was expressed in E. coli BL21(DE3) after induced by IPTG and the recombinant fusion protein was purified with affinity chromatography. Expression amount of NS1 protein was improved by optimizing the inducing parameters. The recombinant NS1 protein is reactive to PPV positive sera in Western blot and ELISA test and therefore can be applicable in differential diagnosis of PPV infections.
文摘2022年美国胸科医师学会(American College of Chest Physicians,ACCP)发布抗栓治疗的围手术期管理指南。该指南主要针对4种临床情况提出抗栓建议,分别是接受维生素K拮抗剂、肝素(heparin)桥接、直接口服抗凝药物及抗血小板药物这4类药物需要手术或侵入性操作患者以及围手术期实验室检测提供指导建议。该文就ACCP围手术期抗栓治疗既往版本指南进行比较,同时结合国内发布的《抗栓治疗病人接受非心脏手术围手术期管理上海专家共识(2021版)》和《中国血栓性疾病防治指南》对该指南进行全面解读,以期为临床围手术期应用抗血栓药物提供参考。
