Earth temperature is one of the most important factors influencing the mechanical properties of frozen soil. Based on the field investigation of the characteristics of ground deformation and ground failure caused by t...Earth temperature is one of the most important factors influencing the mechanical properties of frozen soil. Based on the field investigation of the characteristics of ground deformation and ground failure caused by the M S8 1 earthquake in the west of the Kunlun Mountain Pass, China, the influence of temperature on the dynamic constitutive relationship, dynamic elastic modulus, damping ratio and dynamic strength of frozen soil was quantitatively studied by means of the dynamic triaxial test. Moreover, the characteristics of ground motion on a permafrost site under different temperatures were analyzed for the four profiles of permafrost along the Qinghai Xizang(Tibet) Railway using the time histories of ground motion acceleration with 3 exceedance probabilities of the Kunlun Mountains area. The influences of temperature on the seismic displacement, velocity, acceleration and response spectrum on permafrost ground were studied quantitatively. A scientific basis was presented for earthquake disaster mitigation for engineering foundations, highways and underground engineering in permafrost areas.展开更多
The recombinant adeno-associated viral vector with human coagulation Factor Ⅸ minigene which wasregulated by CMV promoter was constructed. Largequantity of recombinant adeno-associated viral particles (rAAV/ hFⅨ) wa...The recombinant adeno-associated viral vector with human coagulation Factor Ⅸ minigene which wasregulated by CMV promoter was constructed. Largequantity of recombinant adeno-associated viral particles (rAAV/ hFⅨ) was prepared by the HSV/AAV hybrid helper virus method. Southern dot blot assay and QC-PCR indicated that the titer of the virus was 3.6×1012 v.g./mL. It demonstrated that this method can effectively overcome the hurdles of mass production of AAV vector. Followed by anintramuscular injection of viral vectors (7.5×1011 v.g./mouse) in the quadriceps femoris, an elevation of human Factor Ⅸexpression in the plasma of hemophilia B mice was detected (387 ng/mL) and persisted more than 12 weeks. The level of anti-virus antibody in plasma aligned with the Factor Ⅸexpression curve. The QC-PCR method is easier and moreaccurate than traditional dot hybridization fordetermination of the titer of recombinant adeno-associated virus. Moreover, there are no HSV particles existing inproduced AAV assayed by RT-PCR. AAV is the only virus that has been amplified from AAV-injected muscle by PCR.展开更多
文摘Earth temperature is one of the most important factors influencing the mechanical properties of frozen soil. Based on the field investigation of the characteristics of ground deformation and ground failure caused by the M S8 1 earthquake in the west of the Kunlun Mountain Pass, China, the influence of temperature on the dynamic constitutive relationship, dynamic elastic modulus, damping ratio and dynamic strength of frozen soil was quantitatively studied by means of the dynamic triaxial test. Moreover, the characteristics of ground motion on a permafrost site under different temperatures were analyzed for the four profiles of permafrost along the Qinghai Xizang(Tibet) Railway using the time histories of ground motion acceleration with 3 exceedance probabilities of the Kunlun Mountains area. The influences of temperature on the seismic displacement, velocity, acceleration and response spectrum on permafrost ground were studied quantitatively. A scientific basis was presented for earthquake disaster mitigation for engineering foundations, highways and underground engineering in permafrost areas.
基金supported by the National Natural Science Foundation of China(Grant No.30100102).
文摘The recombinant adeno-associated viral vector with human coagulation Factor Ⅸ minigene which wasregulated by CMV promoter was constructed. Largequantity of recombinant adeno-associated viral particles (rAAV/ hFⅨ) was prepared by the HSV/AAV hybrid helper virus method. Southern dot blot assay and QC-PCR indicated that the titer of the virus was 3.6×1012 v.g./mL. It demonstrated that this method can effectively overcome the hurdles of mass production of AAV vector. Followed by anintramuscular injection of viral vectors (7.5×1011 v.g./mouse) in the quadriceps femoris, an elevation of human Factor Ⅸexpression in the plasma of hemophilia B mice was detected (387 ng/mL) and persisted more than 12 weeks. The level of anti-virus antibody in plasma aligned with the Factor Ⅸexpression curve. The QC-PCR method is easier and moreaccurate than traditional dot hybridization fordetermination of the titer of recombinant adeno-associated virus. Moreover, there are no HSV particles existing inproduced AAV assayed by RT-PCR. AAV is the only virus that has been amplified from AAV-injected muscle by PCR.
