目的通过静息态功能磁共振成像(functional magnetic resonance imaging,fMRI)局部一致性(regional homogeneity,ReHo)来分析职业性噪声聋患者脑功能区神经重塑情况,进而探讨职业性噪声聋患者脑功能改变的异常病理生理学机制。方法前瞻...目的通过静息态功能磁共振成像(functional magnetic resonance imaging,fMRI)局部一致性(regional homogeneity,ReHo)来分析职业性噪声聋患者脑功能区神经重塑情况,进而探讨职业性噪声聋患者脑功能改变的异常病理生理学机制。方法前瞻性选取烟台市烟台山医院南院职业病科2017年1月至2019年12月收治的职业性噪声聋确诊患者55名(年龄范围35~60岁),同时段选取基本情况相匹配的健康对照组38名;收集两组研究对象的基本资料,对所有受试者行常规颅脑、内耳、全脑三维快速扰相梯度回波(3D-FSPGR)、静息态血氧水平依赖的功能磁共振成像(BOLD-fMRI)检查,利用DPARSF分析软件将两组受试者的ReHo值进行独立样本t检验,FDR校正后提取差异有统计学意义的脑区进行统计学分析,采用双变量Pearson相关检验来分析ReHo值与噪声暴露时间、汉密尔顿焦虑量表(HAMA)评分、双耳语频平均听阈的相关关系。结果两组受试者的年龄、受教育年限、MMSE评分差异均无统计学意义(均P>0.05);噪声聋组HAMA评分(4.76±1.72)分,对照组为(3.79±1.07)分,差异有统计学意义(P<0.05),职业噪声暴露时间与HAMA评分呈负相关。噪声聋组与对照组相比,ReHo值降低的脑区包括左侧额中回、右侧角回、左侧枕中回、左侧角回、右侧额中回,差异均有统计学意义(均P<0.05);没有发现ReHo值增高的脑区;ReHo值改变有差异的脑区与噪声暴露时间、HAMA评分、双耳语频平均听阈均无明显相关性。结论职业性噪声聋患者在静息态下fMRI显示非听觉皮层脑区存在多处脑区神经元活动和相邻脑区同步性降低的现象,提示脑部功能出现异常,有一定程度的功能重组。展开更多
阐述核心素养的发展和内涵,以及思维可视化的定义、途径和重要意义。以译林版高中《英语》必修一Unit 1 Back to School为例,将括号图、双气泡图等思维可视化工具应用于教学实践,分析思维可视化工具对英语学科核心素养培养的作用。研究...阐述核心素养的发展和内涵,以及思维可视化的定义、途径和重要意义。以译林版高中《英语》必修一Unit 1 Back to School为例,将括号图、双气泡图等思维可视化工具应用于教学实践,分析思维可视化工具对英语学科核心素养培养的作用。研究表明,在高中英语教学中,思维可视化理念的融合,思维可视化工具的应用,有利于促进学生英语学科核心素养的发展。展开更多
Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gaine...Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gained the preference since reliable facilitative and inexpensive. In order to promote the DGGE further development and make it become a general analysis technique earlier, the key procedures and the advances in microbial communities analysis of DGGE were introduced in this paper.展开更多
The denitrifying sulfide removal(DSR) process has recently been studied extensively from an engineering perspective. However, the importance of microbial communities of this process was generally underestimated. In th...The denitrifying sulfide removal(DSR) process has recently been studied extensively from an engineering perspective. However, the importance of microbial communities of this process was generally underestimated. In this study, the microbial community structure of a lab-scale DSR reactor was characterized in order to provide a comprehensive insight into the key microbial groups in DSR system. Results from high-throughput sequencing analysis revealed that the fraction of autotrophic denitrifiers increased from 2.34 % to 10.93% and 44.51% in the DSR system when the influent Na Cl increased from 0 g/L, to 4 g/L and 30 g/L, respectively. On the contrary, the fraction of heterotrophic denitrifiers decreased from 61.74% to 39.57%, and 24.12%, respectively. Azoarcus and Thiobacillus were the main autotrophic denitrifiers, and Thauera was the main hetetrophic denitrifier during the whole process. This study could be useful for better understanding the interaction between autotrophs and heterotrophs in DSR system.展开更多
文摘目的通过静息态功能磁共振成像(functional magnetic resonance imaging,fMRI)局部一致性(regional homogeneity,ReHo)来分析职业性噪声聋患者脑功能区神经重塑情况,进而探讨职业性噪声聋患者脑功能改变的异常病理生理学机制。方法前瞻性选取烟台市烟台山医院南院职业病科2017年1月至2019年12月收治的职业性噪声聋确诊患者55名(年龄范围35~60岁),同时段选取基本情况相匹配的健康对照组38名;收集两组研究对象的基本资料,对所有受试者行常规颅脑、内耳、全脑三维快速扰相梯度回波(3D-FSPGR)、静息态血氧水平依赖的功能磁共振成像(BOLD-fMRI)检查,利用DPARSF分析软件将两组受试者的ReHo值进行独立样本t检验,FDR校正后提取差异有统计学意义的脑区进行统计学分析,采用双变量Pearson相关检验来分析ReHo值与噪声暴露时间、汉密尔顿焦虑量表(HAMA)评分、双耳语频平均听阈的相关关系。结果两组受试者的年龄、受教育年限、MMSE评分差异均无统计学意义(均P>0.05);噪声聋组HAMA评分(4.76±1.72)分,对照组为(3.79±1.07)分,差异有统计学意义(P<0.05),职业噪声暴露时间与HAMA评分呈负相关。噪声聋组与对照组相比,ReHo值降低的脑区包括左侧额中回、右侧角回、左侧枕中回、左侧角回、右侧额中回,差异均有统计学意义(均P<0.05);没有发现ReHo值增高的脑区;ReHo值改变有差异的脑区与噪声暴露时间、HAMA评分、双耳语频平均听阈均无明显相关性。结论职业性噪声聋患者在静息态下fMRI显示非听觉皮层脑区存在多处脑区神经元活动和相邻脑区同步性降低的现象,提示脑部功能出现异常,有一定程度的功能重组。
文摘阐述核心素养的发展和内涵,以及思维可视化的定义、途径和重要意义。以译林版高中《英语》必修一Unit 1 Back to School为例,将括号图、双气泡图等思维可视化工具应用于教学实践,分析思维可视化工具对英语学科核心素养培养的作用。研究表明,在高中英语教学中,思维可视化理念的融合,思维可视化工具的应用,有利于促进学生英语学科核心素养的发展。
基金Supported by Program for New Century Excellent Talents in Ministry of Education(2005800612)
文摘Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gained the preference since reliable facilitative and inexpensive. In order to promote the DGGE further development and make it become a general analysis technique earlier, the key procedures and the advances in microbial communities analysis of DGGE were introduced in this paper.
基金supported by the National Natural Science Foundation of China under Grant No.21307160the Natural Science Foundation of Shandong Province under Grant No.ZR2013EEQ030the Fundamental Research Funds for the Central Universities under Grant No.R1404005A
文摘The denitrifying sulfide removal(DSR) process has recently been studied extensively from an engineering perspective. However, the importance of microbial communities of this process was generally underestimated. In this study, the microbial community structure of a lab-scale DSR reactor was characterized in order to provide a comprehensive insight into the key microbial groups in DSR system. Results from high-throughput sequencing analysis revealed that the fraction of autotrophic denitrifiers increased from 2.34 % to 10.93% and 44.51% in the DSR system when the influent Na Cl increased from 0 g/L, to 4 g/L and 30 g/L, respectively. On the contrary, the fraction of heterotrophic denitrifiers decreased from 61.74% to 39.57%, and 24.12%, respectively. Azoarcus and Thiobacillus were the main autotrophic denitrifiers, and Thauera was the main hetetrophic denitrifier during the whole process. This study could be useful for better understanding the interaction between autotrophs and heterotrophs in DSR system.
