Despite the diverse roles of tripartite motif(Trim)-containing proteins in the regulation of autophagy,the innate immune response,and cell differentiation,their roles in skeletal diseases are largely unknown.We recent...Despite the diverse roles of tripartite motif(Trim)-containing proteins in the regulation of autophagy,the innate immune response,and cell differentiation,their roles in skeletal diseases are largely unknown.We recently demonstrated that Trim21 plays a crucial role in regulating osteoblast(OB)differentiation in osteosarcoma.However,how Trim21 contributes to skeletal degenerative disorders,including osteoporosis,remains unknown.First,human and mouse bone specimens were evaluated,and the results showed that Trim21 expression was significantly elevated in bone tissues obtained from osteoporosis patients.Next,we found that global knockout of the Trim21 gene(KO,Trim2^(1-/-))resulted in higher bone mass compared to that of the control littermates.We further demonstrated that loss of Trim21 promoted bone formation by enhancing the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and elevating the activity of OBs;moreover,Trim21 depletion suppressed osteoclast(OC)formation of RAW264.7 cells.In addition,the differentiation of OCs from bone marrow-derived macrophages(BMMs)isolated from Trim21^(-/-)and Ctsk-cre;Trim21^(f/f)mice was largely compromised compared to that of the littermate control mice.Mechanistically,YAP1/β-catenin signaling was identified and demonstrated to be required for the Trim21-mediated osteogenic differentiation of BMSCs.More importantly,the loss of Trim21 prevented ovariectomy(OVX)-and lipopolysaccharide(LPS)-induced bone loss in vivo by orchestrating the coupling of OBs and OCs through YAP1 signaling.Our current study demonstrated that Trim21 is crucial for regulating OB-mediated bone formation and OC-mediated bone resorption,thereby providing a basis for exploring Trim21 as a novel dual-targeting approach for treating osteoporosis and pathological bone loss.展开更多
Vibrio parahaemolyticus,the leading cause of seafood-borne gastroenteritis,has the ability to form biofilms on biotic and abiotic surfaces.Biofilm formation is a complicated process involving many specific structures ...Vibrio parahaemolyticus,the leading cause of seafood-borne gastroenteritis,has the ability to form biofilms on biotic and abiotic surfaces.Biofilm formation is a complicated process involving many specific structures and regulatory processes.The most significant of the structures and processes include polar and lateral flagella,mannose-sensitive hemagglutinin type Ⅳ pili,chitin-regulated pili, capsular polysaccharide (CPS),exopolysaccharide (EPS),cyclic-di-GMP,and quorum sensing[1].Some transcriptional regu[ators,such as OxyR[2], VPA0606[3],and CpsR[4],are involved in the regulation of V.parahaemolyticus biofilms.However, the full details of the regulation of V. parahaemolyticus biofilm formation are unknown.展开更多
目的通过对金山区2015-2017年流感监测结果进行分析,了解流感流行特征,为流感防控工作提供科学的依据。方法收集2015-2017年金山区流感样病例(influenza like illness,ILI)监测数据和实验室病原学检测数据进行整理分析。结果2015-2017...目的通过对金山区2015-2017年流感监测结果进行分析,了解流感流行特征,为流感防控工作提供科学的依据。方法收集2015-2017年金山区流感样病例(influenza like illness,ILI)监测数据和实验室病原学检测数据进行整理分析。结果2015-2017年共计监测流感样病例29013例,占门急诊就诊病例总数的百分比(ILI%)为2.23%,共采集流感样病例鼻咽拭标本3197份,流感病毒核酸检测总阳性率为23.52%(752/3197)。2015年春季高峰以乙型(Yamagata)为主,2015年夏季高峰以季节性A(H3N2)为主,2015-2016度的冬春季高峰以新型甲型H1N1和乙型共同流行。2016年无明显的夏季高峰,2016-2017年度的冬春季高峰以季节性A(H3N2)为主,散在伴有新型甲型H1N1和乙型流行。2017年夏季高峰以季节性A(H3N2)为主,而冬季高峰以乙型(Yamagata)为主。ILI%与流感病毒核酸检出阳性率呈正相关,Spearman秩相关分析结果显示两者高度相关(r=0.505,P<0.05)。男、女之间流感病毒核酸检出阳性率差异无统计学意义(χ^2=1.123,P>0.05),不同年龄组核酸检出阳性率差异有统计学意义(χ^2=52.254,P<0.05),60岁以上组阳性率最高30.23%,0~岁组阳性率最低13.50%。结论金山区流感流行一般分为夏季和冬春季两个流行高峰,发病人群以15岁以下儿童青少年为主。流感病毒优势毒株交替变化并呈现一定规律,ILI流行特征与流感病毒活动强度基本一致。展开更多
基金supported by the Natural Science Foundation with grants from the National Key R&D Program of China(2018YFC2002500)National Natural Science Foundation of China(81602360,82072470,82350003,92049201)+6 种基金Key Laboratory Construction Project of Guangzhou Science and Technology Bureau(202102100007)supported by the Clinical Frontier Technology Program of the First Affiliated Hospital of Jinan University,China(No.JNU1AF-CFTP-2022-a01221)Natural Science Foundation of Guangdong Province(2021A1515012154,2019A1515011082,2017A030313665,2018A030313544,2020B1515120038)Science and Technology Projects in Guangzhou(201707010493,202102010069)Macao Foundation for Development of Science and Technology(0029/2019/A)Youth Talent Support Project of Guangzhou Association for Science&Technology(X20200301018)pilot project of clinical collaboration from National Administration of Traditional Chinese Medicine and National Health Commission of the People’s Republic of China and Logistics Support Department of the Central Military Commission。
文摘Despite the diverse roles of tripartite motif(Trim)-containing proteins in the regulation of autophagy,the innate immune response,and cell differentiation,their roles in skeletal diseases are largely unknown.We recently demonstrated that Trim21 plays a crucial role in regulating osteoblast(OB)differentiation in osteosarcoma.However,how Trim21 contributes to skeletal degenerative disorders,including osteoporosis,remains unknown.First,human and mouse bone specimens were evaluated,and the results showed that Trim21 expression was significantly elevated in bone tissues obtained from osteoporosis patients.Next,we found that global knockout of the Trim21 gene(KO,Trim2^(1-/-))resulted in higher bone mass compared to that of the control littermates.We further demonstrated that loss of Trim21 promoted bone formation by enhancing the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and elevating the activity of OBs;moreover,Trim21 depletion suppressed osteoclast(OC)formation of RAW264.7 cells.In addition,the differentiation of OCs from bone marrow-derived macrophages(BMMs)isolated from Trim21^(-/-)and Ctsk-cre;Trim21^(f/f)mice was largely compromised compared to that of the littermate control mice.Mechanistically,YAP1/β-catenin signaling was identified and demonstrated to be required for the Trim21-mediated osteogenic differentiation of BMSCs.More importantly,the loss of Trim21 prevented ovariectomy(OVX)-and lipopolysaccharide(LPS)-induced bone loss in vivo by orchestrating the coupling of OBs and OCs through YAP1 signaling.Our current study demonstrated that Trim21 is crucial for regulating OB-mediated bone formation and OC-mediated bone resorption,thereby providing a basis for exploring Trim21 as a novel dual-targeting approach for treating osteoporosis and pathological bone loss.
基金supported by the National Natural Science Foundation of China [81601809]the Natural Science Foundation of Jiangsu Province [BK20160505]
文摘Vibrio parahaemolyticus,the leading cause of seafood-borne gastroenteritis,has the ability to form biofilms on biotic and abiotic surfaces.Biofilm formation is a complicated process involving many specific structures and regulatory processes.The most significant of the structures and processes include polar and lateral flagella,mannose-sensitive hemagglutinin type Ⅳ pili,chitin-regulated pili, capsular polysaccharide (CPS),exopolysaccharide (EPS),cyclic-di-GMP,and quorum sensing[1].Some transcriptional regu[ators,such as OxyR[2], VPA0606[3],and CpsR[4],are involved in the regulation of V.parahaemolyticus biofilms.However, the full details of the regulation of V. parahaemolyticus biofilm formation are unknown.
文摘目的通过对金山区2015-2017年流感监测结果进行分析,了解流感流行特征,为流感防控工作提供科学的依据。方法收集2015-2017年金山区流感样病例(influenza like illness,ILI)监测数据和实验室病原学检测数据进行整理分析。结果2015-2017年共计监测流感样病例29013例,占门急诊就诊病例总数的百分比(ILI%)为2.23%,共采集流感样病例鼻咽拭标本3197份,流感病毒核酸检测总阳性率为23.52%(752/3197)。2015年春季高峰以乙型(Yamagata)为主,2015年夏季高峰以季节性A(H3N2)为主,2015-2016度的冬春季高峰以新型甲型H1N1和乙型共同流行。2016年无明显的夏季高峰,2016-2017年度的冬春季高峰以季节性A(H3N2)为主,散在伴有新型甲型H1N1和乙型流行。2017年夏季高峰以季节性A(H3N2)为主,而冬季高峰以乙型(Yamagata)为主。ILI%与流感病毒核酸检出阳性率呈正相关,Spearman秩相关分析结果显示两者高度相关(r=0.505,P<0.05)。男、女之间流感病毒核酸检出阳性率差异无统计学意义(χ^2=1.123,P>0.05),不同年龄组核酸检出阳性率差异有统计学意义(χ^2=52.254,P<0.05),60岁以上组阳性率最高30.23%,0~岁组阳性率最低13.50%。结论金山区流感流行一般分为夏季和冬春季两个流行高峰,发病人群以15岁以下儿童青少年为主。流感病毒优势毒株交替变化并呈现一定规律,ILI流行特征与流感病毒活动强度基本一致。
