Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var.(RCC,♀)×Megalobrama amblycephala(BSB,♂),containing four sets of RCC chro...Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var.(RCC,♀)×Megalobrama amblycephala(BSB,♂),containing four sets of RCC chromosomes.However,the molecular mechanism underlying the determination of sex in this species remains largely unknown.Currently,there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species.In this study,25,801,677 SNPs(Singlenucleotide polymorphism)and 6,210,306 Indels(insertion-deletion)were obtained from whole-genome resequencing of 100 individuals(including 50 female and 50 male).Further identification confirmed the candidate chromosomes as Chr46B,with the sex-determining region located at Chr46B:22,500,000‒22,800,000 bp.Based on the male-specific insertion(26 bp)within the candidate sex-determining region,a pair of sex-specific molecular markers has been identified.In addition,based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis,ADAM10,AQP9 and tc1a were identified as candidate sex-determining genes.These findings provide a robust foundation for investigating sex determination mechanisms in fish,the evolution of sex chromosomes,and the development of monosex populations.展开更多
Based on the full vector complex coupled mode theory, a detailed analysis is made on the transmission spectrum characteristics of tilted long period fiber gratings. New transmission peaks are observed, which are locat...Based on the full vector complex coupled mode theory, a detailed analysis is made on the transmission spectrum characteristics of tilted long period fiber gratings. New transmission peaks are observed, which are located beside the long wavelength side of each transmission peak in the transmission spectrum of normal long period fiber gratings. The emerging transmission peaks are quite sensitive to both the grating tilted angle and the surrounding refractive index, and the corresponding relationship is discussed. Furthermore, a novel sensing characteristic is investigated about the tilted long period fiber gratings, which is related to the transmission resonant wavelength and peak amplitude.展开更多
基金financially supported by the National Natural Science Foundation of China(32172972)the Science and Technology Innovation Program of Hunan Province(2021RC4028)+1 种基金the Special Funds for Construction of Innovative Provinces in Hunan Province(2021NK1010)Special Science Found of Nansha-South China Agricultural University Fishery Research Institute,Guangzhou,the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province,the earmarked fund for HARS(HARS-07)。
文摘Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var.(RCC,♀)×Megalobrama amblycephala(BSB,♂),containing four sets of RCC chromosomes.However,the molecular mechanism underlying the determination of sex in this species remains largely unknown.Currently,there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species.In this study,25,801,677 SNPs(Singlenucleotide polymorphism)and 6,210,306 Indels(insertion-deletion)were obtained from whole-genome resequencing of 100 individuals(including 50 female and 50 male).Further identification confirmed the candidate chromosomes as Chr46B,with the sex-determining region located at Chr46B:22,500,000‒22,800,000 bp.Based on the male-specific insertion(26 bp)within the candidate sex-determining region,a pair of sex-specific molecular markers has been identified.In addition,based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis,ADAM10,AQP9 and tc1a were identified as candidate sex-determining genes.These findings provide a robust foundation for investigating sex determination mechanisms in fish,the evolution of sex chromosomes,and the development of monosex populations.
文摘Based on the full vector complex coupled mode theory, a detailed analysis is made on the transmission spectrum characteristics of tilted long period fiber gratings. New transmission peaks are observed, which are located beside the long wavelength side of each transmission peak in the transmission spectrum of normal long period fiber gratings. The emerging transmission peaks are quite sensitive to both the grating tilted angle and the surrounding refractive index, and the corresponding relationship is discussed. Furthermore, a novel sensing characteristic is investigated about the tilted long period fiber gratings, which is related to the transmission resonant wavelength and peak amplitude.
