Background:Alzheimer’sdisease(AD)is a prevalent neurodegenerative disorder causing progressive dementia.Research suggests that microRNAs(miRNAs)could serve as biomarkers and therapeutic targets for AD.Reduced levels ...Background:Alzheimer’sdisease(AD)is a prevalent neurodegenerative disorder causing progressive dementia.Research suggests that microRNAs(miRNAs)could serve as biomarkers and therapeutic targets for AD.Reduced levels of miR-137 have been observed in the brains of AD patients,but its specific role and down stream mechanisms remain unclear.This study sought to examine the therapeutic potential of miR-137-5p agomir in alleviating cognitive dysfunction induced in AD models and explore its potential mechanisms.Methods:This study utilized bioinformatic analysis and a dual-l uciferase reporter assay to investigate the relationship between miR-137-5p and ubiquitin-specific peptidase 30(USP30).In vitro experiments were conducted using SH-SY5Y cells to assess the impact of miR-137-5p on Aβ1-42 neurotoxicity.In vivo experiments on AD mice evaluated the effects of miR-137-5p on cognition,Aβ1-42 deposition,Tau hyperphosphorylation,and neuronal apoptosis,as well as its influence on USP30 levels.Results:It was discovered that miR-137-5p mimics efficiently counteract Aβ1-42 neurotoxicity in SH-SY5Y cells,a protective effect that is negated by USP30 overexpression.In vivo experiments demonstrated that miR-137-5p enhances the cognition and mobility of AD mice,significantly reducing Aβ1-42 deposition,Tau hyperphosphorylation,and neuronal apoptosis within the hippocampus and cortex regions.Mechanistically,miR-137-5p significantly suppresses USP30 levels in mice,though USP30 overexpression partially buffers against miR-137-5p-i nduced AD symptom improvement.Conclusion:Our study proposes that miR-137-5p,by instigating the downregulation of USP30,has the potential to act as a novel and promising therapeutic target for AD.展开更多
Objectives:To translate the Child Intermittent Exotropia Questionnaire(Child-IXTQ)from English to Chinese and to evaluate its reliability and validity in Chinese childhood strabismus patients.Method:A consecutive samp...Objectives:To translate the Child Intermittent Exotropia Questionnaire(Child-IXTQ)from English to Chinese and to evaluate its reliability and validity in Chinese childhood strabismus patients.Method:A consecutive sample of 143 child strabismus patients was recruited from the Department of Ophthalmology at a medical center in Southwest China.In addition,100 visually normal adults and 100 patients with other eye diseases were recruited.The Brislin translation model was followed to develop the Chinese version of Child-IXTQ.Reliability was established using Cronbach's a and test-retest.Validity was established encompassing content validity,construct validity,convergent validity,criterion-related validity and discriminative validity.Results:The correlation coefficients between each item score with the total score ranged from 0.370 to 0.813.Two subscales were extracted by principal component analysis,with a content validity of 0.91.The correlation coefficients between two factor scores with the total score were 0.709 and 0.939.Criterion-related validity was estimated by the correlating the Child-IXTQ with the PedsQL 4.0,and the correlation coefficient was 0.522.In addition,the Child-IXTQ discriminated accurately between strabismus patients and normal children,or children with other eye diseases that possessed good discriminative validity.Cronbach's a coefficient for the internal consistency was 0.907 and the test-retest reliability was 0.962.Conclusions:Our study indicates that the Child-IXTQ is a reliable and valid instrument for assessing the health-related quality of life among children with strabismus.展开更多
Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with s...Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with solar energy to realize the large-scale and low-cost treatment of highly mineralized mine water in the western coalproducing region of China.In this study,highly mineralized mine water from the Ningdong area of China was subjected to vacuum MD(VMD)using polyvinylidene fluoride hollow-fiber membranes.The optimal operation parameters of VMD were determined by response surface optimization.Subsequently,the feasibility of VMD for treating highly mineralized mine water was explored.The fouling behavior observed during VMD was further investigated by scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDS).Under the optimal parameters(pressure=-0.08 MPa,temperature=70℃,and feed flow rate=1.5 L/min),the maximum membrane flux was 8.85 kg/(m^(2) h),and the desalination rate was 99.7%.Membrane fouling could be divided into three stages:membrane wetting,crystallization,and fouling layer formation.Physical cleaning restored the flux and salt rejection rate to 94%and 97%of the initial values,respectively;however,the cleaning interval and cleaning efficiency decreased as the VMD run time increased.SEM-EDS analysis revealed that the reduction in flux was caused by the precipitation of CaCO_(3).The findings also demonstrated that the membrane wetting could be attributed to the formation of NaCl on the cross section and outer surface of the membrane.Overall,the results confirm the feasibility of MD for treating mine water and provide meaningful guidance for the industrial application of MD.展开更多
Before spring ploughing in 2019,the representative fields of the 8^(th) Division were selected,and residual film at different depths of soil in three areas of the 8^(th) Division was collected. Through the analysis on...Before spring ploughing in 2019,the representative fields of the 8^(th) Division were selected,and residual film at different depths of soil in three areas of the 8^(th) Division was collected. Through the analysis on the weight and amount of residual film at different depths of soil,it was found that the average content of residual film in the 8^(th) Division was 104 kg/hm^2. From high to low,the content sequence of residual film in the three areas was Anjihai area,Mosuowan area and Xiayedi area. The average amount of residual film collected from the cotton field in the three areas was greater than that from the corn field. In the three areas,the content of residual film in the cotton field at the depth of 0-10 and 11-30 cm was higher than that in the corn field,while the content of residual film at the depth of 31-50 cm in the corn field was higher than that in the cotton field.展开更多
Numerical simulation methods for unsteady vortex field of helicopter rotor with high resolution and low dissipation TENO8-AA primitive variables reconstruction schemes are established based on moving-embedded grid and...Numerical simulation methods for unsteady vortex field of helicopter rotor with high resolution and low dissipation TENO8-AA primitive variables reconstruction schemes are established based on moving-embedded grid and Navier-Stokes equations.Firstly,the Targeted Essentially Non-Oscillatory(TENO)scheme are developed by employing ENO-like candidate stencil selection strategy,and the candidate stencil is adopted with optimal weight in smooth region while it is discarded completely in discontinuous region,which reduces the dissipation and dispersion errors and approaches better spectral properties.Then,the aerodynamic characteristics of Helishape-7A model rotor in Blade Vortex Interaction(BVI)state and the flowfield of Lynx rotor in hover are simulated,which validates that the blade tip vortex trajectory with larger wake age and more details of vortex can be captured by TENO8-AA scheme with only a quarter of grid points and half time comparing to WENO-JS scheme.Moreover,the simulation accuracy of thrust coefficient is improved by up to 36%.Finally,the analyses for BVI and aeroacoustic characteristics of Operational Loads Survey(OLS)rotor are conducted,and the different forms of interaction mechanism are explored,such as oblique and parallel interactions.The results indicate that TENO scheme not only ensures the resolution of simulation in discontinuous region,but also minimizes the numerical dissipation in smooth region dominated by blade tip vortex.Therefore,the acoustic pressure peak prediction error of rotor in BVI state is significantly reduced to 5.6%and 0.8%at two microphone locations,respectively.展开更多
Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug...Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug delivery.As the growing concern for human and environmental safety,selective detection of metal ions has been paid more and more attention.GQDs,as nanoparticles with superior optical properties,have been attracting growing attention in the field of metal ions detection.In this work,glutathione(GSH)functionalized boron doped graphene quantum dots(B-GQDs@GSH)were successfully synthesized with stable bright blue fluorescence and has been used for the detection of Fe^(3+).A good linear relationship between 1/(F_(0)-F)and 1/c with the concentration ranging from 0.70 to 53μmol/L was obtained with a detection limit of 5.5 nmol/L.The application of B-GQDs@GSH for Fe^(3+)detection in water samples was demonstrated and the quenching mechanism was further explored.Due to low cytotoxicity and favorable biocompatibility,B-GQDs@GSH were successfully applied for cell fluorescence imaging and intracellular determination of Fe^(3+).展开更多
The objective of this study was to investigate the nitritation performance in a biofilm reactor for treating domestic wastewater.The reactor was operated in continuous feed mode from phases 1 to 3.The dissolved oxygen...The objective of this study was to investigate the nitritation performance in a biofilm reactor for treating domestic wastewater.The reactor was operated in continuous feed mode from phases 1 to 3.The dissolved oxygen(DO)was controlled at 3.5–7 mg/L throughout the experiment.The biofilm reactor showed excellent nitritation performance after the inoculation of nitrifying sludge,with the hydraulic retention time being reduced from 24 to 7 hr.Above 90%nitrite accumulation ratio(NAR)was maintained in phase 1.Afterwards,nitratation occurred with the low NH_4^+–N concentration in the reactor.The improvement of NH_4^+–N concentration to 20–35 mg/L had a limited effect on the recovery of nitritation.However,nitritation recovered rapidly when sequencing batch feed mode was adopted in phase 4,with the effluent NH_4^+-N concentration above 7 mg/L.The improvement of ammonia oxidizing bacteria(AOB)activity and the combined inhibition effect of free ammonia(FA)and free nitrous acid(FNA)on the nitrite oxidizing bacteria(NOB)were two key factors for the rapid recovery of nitritation.Sludge activity was obtained in batch tests.The results of batch tests had a good relationship with the long term operation performance of the biofilm reactor.展开更多
The aim of the present work was to evaluate the anaerobic ammonium oxidation(anammox) activity of simultaneous partial nitrification, anammox and denitrification(SNAD) biofilm with different substrate concentrations a...The aim of the present work was to evaluate the anaerobic ammonium oxidation(anammox) activity of simultaneous partial nitrification, anammox and denitrification(SNAD) biofilm with different substrate concentrations and p H values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH_(4^-)^+N removal rate of anammox process remained 87.4% of the maximum rate with the NO_(2^-)^-N concentration of 100 mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia(FA) conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the p H value of 7.0, the r max, Ks and K_I of ammonium were 0.209 kg NO_(2^-)^-N/kg VSS/day, 9.5 mg/L and 422 mg/L, respectively. The suitable p H ranges for anammox process were 5.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions.展开更多
Thyroid carcinoma is one of the most common endocrine malignant diseases worldwide.With the rapid development of medical technology,early and effective diagnostic methods could be able to improve the survival rate and...Thyroid carcinoma is one of the most common endocrine malignant diseases worldwide.With the rapid development of medical technology,early and effective diagnostic methods could be able to improve the survival rate and quality of life of patients suffering from the disease.Considering the complexity of cancer,some specific detection method is desired for diagnosis and treatment.Mass spectrometry imaging(MSI)is an emerging technique for acquiring molecular information from biological tissues without staining and labeling,including qualitative,quantitative and spatial distribution information.Over the past several decades,MSI has been widely used for pharmacological monitoring,biomolecular imaging of cells and tissues.In this review,we introduce the tumor progression and histological characteristics of thyroid cancer,and focus mainly on the preparation of biological specimens for MSI and mass spectrometry(MS)analysis,as well as the recent progress in MS and MSI-based thyroid cancer research.This review thoroughly discusses the importance of MS and MSI for clinical diagnosis,identification and prognosis of thyroid cancer,and provides some new clues for molecular mechanisms research and tumor metastasis.展开更多
A hertz-linewidth ultra-stable laser(USL), which will be used to detect the clock transition line, in a strontium optical clock will be launched into the China Space Station(CSS) in late 2022. As the core of the USL, ...A hertz-linewidth ultra-stable laser(USL), which will be used to detect the clock transition line, in a strontium optical clock will be launched into the China Space Station(CSS) in late 2022. As the core of the USL, an interference-filter-based externalcavity diode laser(IF-ECDL) was developed. The IF-ECDL has a compact, stable, and environmentally insensitive design.Performances of the IF-ECDL are presented. The developed IF-ECDL can pass the aerospace environmental tests, indicating that the IF-ECDL can be suitable for space missions in the CSS.展开更多
A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized.The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations a...A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized.The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations and a large Stokes shift of 202 nm.Moreover,its practical cell imaging capability for intracellular Ca2+ in the resting- and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.展开更多
基金Liaoning Province Science and Technology Project(grant/award number:2019-BS-221)Shenyang Science and Technology Project(grant/award number:19-112-4-040)。
文摘Background:Alzheimer’sdisease(AD)is a prevalent neurodegenerative disorder causing progressive dementia.Research suggests that microRNAs(miRNAs)could serve as biomarkers and therapeutic targets for AD.Reduced levels of miR-137 have been observed in the brains of AD patients,but its specific role and down stream mechanisms remain unclear.This study sought to examine the therapeutic potential of miR-137-5p agomir in alleviating cognitive dysfunction induced in AD models and explore its potential mechanisms.Methods:This study utilized bioinformatic analysis and a dual-l uciferase reporter assay to investigate the relationship between miR-137-5p and ubiquitin-specific peptidase 30(USP30).In vitro experiments were conducted using SH-SY5Y cells to assess the impact of miR-137-5p on Aβ1-42 neurotoxicity.In vivo experiments on AD mice evaluated the effects of miR-137-5p on cognition,Aβ1-42 deposition,Tau hyperphosphorylation,and neuronal apoptosis,as well as its influence on USP30 levels.Results:It was discovered that miR-137-5p mimics efficiently counteract Aβ1-42 neurotoxicity in SH-SY5Y cells,a protective effect that is negated by USP30 overexpression.In vivo experiments demonstrated that miR-137-5p enhances the cognition and mobility of AD mice,significantly reducing Aβ1-42 deposition,Tau hyperphosphorylation,and neuronal apoptosis within the hippocampus and cortex regions.Mechanistically,miR-137-5p significantly suppresses USP30 levels in mice,though USP30 overexpression partially buffers against miR-137-5p-i nduced AD symptom improvement.Conclusion:Our study proposes that miR-137-5p,by instigating the downregulation of USP30,has the potential to act as a novel and promising therapeutic target for AD.
基金National Natural Science Foundation of China （#90208011, #30300174, #30070856 , #30421005） National Key Basic Research and Development Program of China （#2002CB713802 , #2005CB522700） Shanghai Key Project of Basic Science Research （#04DZ14005 , #04DZ05608）.
基金This study was funded by the Clinical Care Technology Innovation Fund(201202)for projects at the Southwest Hospital.
文摘Objectives:To translate the Child Intermittent Exotropia Questionnaire(Child-IXTQ)from English to Chinese and to evaluate its reliability and validity in Chinese childhood strabismus patients.Method:A consecutive sample of 143 child strabismus patients was recruited from the Department of Ophthalmology at a medical center in Southwest China.In addition,100 visually normal adults and 100 patients with other eye diseases were recruited.The Brislin translation model was followed to develop the Chinese version of Child-IXTQ.Reliability was established using Cronbach's a and test-retest.Validity was established encompassing content validity,construct validity,convergent validity,criterion-related validity and discriminative validity.Results:The correlation coefficients between each item score with the total score ranged from 0.370 to 0.813.Two subscales were extracted by principal component analysis,with a content validity of 0.91.The correlation coefficients between two factor scores with the total score were 0.709 and 0.939.Criterion-related validity was estimated by the correlating the Child-IXTQ with the PedsQL 4.0,and the correlation coefficient was 0.522.In addition,the Child-IXTQ discriminated accurately between strabismus patients and normal children,or children with other eye diseases that possessed good discriminative validity.Cronbach's a coefficient for the internal consistency was 0.907 and the test-retest reliability was 0.962.Conclusions:Our study indicates that the Child-IXTQ is a reliable and valid instrument for assessing the health-related quality of life among children with strabismus.
基金This research was supported by the Open Fund Project of the State Key Laboratory of Water Resources Protection and Utilization in Coal Mining(GJNY-18-73.13).
文摘Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with solar energy to realize the large-scale and low-cost treatment of highly mineralized mine water in the western coalproducing region of China.In this study,highly mineralized mine water from the Ningdong area of China was subjected to vacuum MD(VMD)using polyvinylidene fluoride hollow-fiber membranes.The optimal operation parameters of VMD were determined by response surface optimization.Subsequently,the feasibility of VMD for treating highly mineralized mine water was explored.The fouling behavior observed during VMD was further investigated by scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDS).Under the optimal parameters(pressure=-0.08 MPa,temperature=70℃,and feed flow rate=1.5 L/min),the maximum membrane flux was 8.85 kg/(m^(2) h),and the desalination rate was 99.7%.Membrane fouling could be divided into three stages:membrane wetting,crystallization,and fouling layer formation.Physical cleaning restored the flux and salt rejection rate to 94%and 97%of the initial values,respectively;however,the cleaning interval and cleaning efficiency decreased as the VMD run time increased.SEM-EDS analysis revealed that the reduction in flux was caused by the precipitation of CaCO_(3).The findings also demonstrated that the membrane wetting could be attributed to the formation of NaCl on the cross section and outer surface of the membrane.Overall,the results confirm the feasibility of MD for treating mine water and provide meaningful guidance for the industrial application of MD.
基金Supported by the Shihezi Science and Technology Plan of the Eighth Division(2018RK01)。
文摘Before spring ploughing in 2019,the representative fields of the 8^(th) Division were selected,and residual film at different depths of soil in three areas of the 8^(th) Division was collected. Through the analysis on the weight and amount of residual film at different depths of soil,it was found that the average content of residual film in the 8^(th) Division was 104 kg/hm^2. From high to low,the content sequence of residual film in the three areas was Anjihai area,Mosuowan area and Xiayedi area. The average amount of residual film collected from the cotton field in the three areas was greater than that from the corn field. In the three areas,the content of residual film in the cotton field at the depth of 0-10 and 11-30 cm was higher than that in the corn field,while the content of residual film at the depth of 31-50 cm in the corn field was higher than that in the cotton field.
基金the National Natural Science Foundation of China(Nos.12032012,12072156)the National Key Laboratory Foundation of China(No.61422202103)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD).
文摘Numerical simulation methods for unsteady vortex field of helicopter rotor with high resolution and low dissipation TENO8-AA primitive variables reconstruction schemes are established based on moving-embedded grid and Navier-Stokes equations.Firstly,the Targeted Essentially Non-Oscillatory(TENO)scheme are developed by employing ENO-like candidate stencil selection strategy,and the candidate stencil is adopted with optimal weight in smooth region while it is discarded completely in discontinuous region,which reduces the dissipation and dispersion errors and approaches better spectral properties.Then,the aerodynamic characteristics of Helishape-7A model rotor in Blade Vortex Interaction(BVI)state and the flowfield of Lynx rotor in hover are simulated,which validates that the blade tip vortex trajectory with larger wake age and more details of vortex can be captured by TENO8-AA scheme with only a quarter of grid points and half time comparing to WENO-JS scheme.Moreover,the simulation accuracy of thrust coefficient is improved by up to 36%.Finally,the analyses for BVI and aeroacoustic characteristics of Operational Loads Survey(OLS)rotor are conducted,and the different forms of interaction mechanism are explored,such as oblique and parallel interactions.The results indicate that TENO scheme not only ensures the resolution of simulation in discontinuous region,but also minimizes the numerical dissipation in smooth region dominated by blade tip vortex.Therefore,the acoustic pressure peak prediction error of rotor in BVI state is significantly reduced to 5.6%and 0.8%at two microphone locations,respectively.
基金financially supported by the Natural Science Foundation of Shanxi Province of China(201901D111210)Key Research Project of Science and Technology Plan in Jinzhong-Social Development Projects(Y213003)+1 种基金Special Project of Lvliang for Introduced High-level Science and Technology Talents(2021RC-2-1)Transverse Scientific Research Project of Shanxi Taiyuan Pharmaceutical Co.Ltd(2F022022006)
文摘Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug delivery.As the growing concern for human and environmental safety,selective detection of metal ions has been paid more and more attention.GQDs,as nanoparticles with superior optical properties,have been attracting growing attention in the field of metal ions detection.In this work,glutathione(GSH)functionalized boron doped graphene quantum dots(B-GQDs@GSH)were successfully synthesized with stable bright blue fluorescence and has been used for the detection of Fe^(3+).A good linear relationship between 1/(F_(0)-F)and 1/c with the concentration ranging from 0.70 to 53μmol/L was obtained with a detection limit of 5.5 nmol/L.The application of B-GQDs@GSH for Fe^(3+)detection in water samples was demonstrated and the quenching mechanism was further explored.Due to low cytotoxicity and favorable biocompatibility,B-GQDs@GSH were successfully applied for cell fluorescence imaging and intracellular determination of Fe^(3+).
基金funded by the National Water Pollution Control and Management Technology Major Projects(No.2014ZX 07201-011)the Beijing Natural Science Foundation(No.8122005)of Chinathe Beijing Municipal EducationCommission General Program(No.KM2012-10005028)
文摘The objective of this study was to investigate the nitritation performance in a biofilm reactor for treating domestic wastewater.The reactor was operated in continuous feed mode from phases 1 to 3.The dissolved oxygen(DO)was controlled at 3.5–7 mg/L throughout the experiment.The biofilm reactor showed excellent nitritation performance after the inoculation of nitrifying sludge,with the hydraulic retention time being reduced from 24 to 7 hr.Above 90%nitrite accumulation ratio(NAR)was maintained in phase 1.Afterwards,nitratation occurred with the low NH_4^+–N concentration in the reactor.The improvement of NH_4^+–N concentration to 20–35 mg/L had a limited effect on the recovery of nitritation.However,nitritation recovered rapidly when sequencing batch feed mode was adopted in phase 4,with the effluent NH_4^+-N concentration above 7 mg/L.The improvement of ammonia oxidizing bacteria(AOB)activity and the combined inhibition effect of free ammonia(FA)and free nitrous acid(FNA)on the nitrite oxidizing bacteria(NOB)were two key factors for the rapid recovery of nitritation.Sludge activity was obtained in batch tests.The results of batch tests had a good relationship with the long term operation performance of the biofilm reactor.
基金supported by the National Water Pollution Control and Management Technology Major Projects(No.2014ZX 07201-011)and(No.2015ZX 07202-013)the National Natural Science Foundation of China(No.51308010)the Research Fund for the Doctoral Program of Higher Education(No.20131103120017)
文摘The aim of the present work was to evaluate the anaerobic ammonium oxidation(anammox) activity of simultaneous partial nitrification, anammox and denitrification(SNAD) biofilm with different substrate concentrations and p H values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH_(4^-)^+N removal rate of anammox process remained 87.4% of the maximum rate with the NO_(2^-)^-N concentration of 100 mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia(FA) conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the p H value of 7.0, the r max, Ks and K_I of ammonium were 0.209 kg NO_(2^-)^-N/kg VSS/day, 9.5 mg/L and 422 mg/L, respectively. The suitable p H ranges for anammox process were 5.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions.
基金The Natural Science Foundation of Guangdong Province,China(2021A1515010171)Natural Science Foundation of Shanxi Province of China(201901D111210)+2 种基金2019 Platform Base Special Project of Shanxi Province(201905D121002)Shanxi Medical University Innovation and Entrepreneurship Fund for College Students(2020181)Shenzhen Science and Technology Innovation Commission(KCXFZ202002011008124)
文摘Thyroid carcinoma is one of the most common endocrine malignant diseases worldwide.With the rapid development of medical technology,early and effective diagnostic methods could be able to improve the survival rate and quality of life of patients suffering from the disease.Considering the complexity of cancer,some specific detection method is desired for diagnosis and treatment.Mass spectrometry imaging(MSI)is an emerging technique for acquiring molecular information from biological tissues without staining and labeling,including qualitative,quantitative and spatial distribution information.Over the past several decades,MSI has been widely used for pharmacological monitoring,biomolecular imaging of cells and tissues.In this review,we introduce the tumor progression and histological characteristics of thyroid cancer,and focus mainly on the preparation of biological specimens for MSI and mass spectrometry(MS)analysis,as well as the recent progress in MS and MSI-based thyroid cancer research.This review thoroughly discusses the importance of MS and MSI for clinical diagnosis,identification and prognosis of thyroid cancer,and provides some new clues for molecular mechanisms research and tumor metastasis.
基金This work was supported by the National Key R&D Program of China(No.2020YFC2201300)the National Natural Science Foundation of China(No.11903041)。
文摘A hertz-linewidth ultra-stable laser(USL), which will be used to detect the clock transition line, in a strontium optical clock will be launched into the China Space Station(CSS) in late 2022. As the core of the USL, an interference-filter-based externalcavity diode laser(IF-ECDL) was developed. The IF-ECDL has a compact, stable, and environmentally insensitive design.Performances of the IF-ECDL are presented. The developed IF-ECDL can pass the aerospace environmental tests, indicating that the IF-ECDL can be suitable for space missions in the CSS.
基金supported by the National Natural Science Foundation of China(Nos.21175086 and 21175087)
文摘A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized.The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations and a large Stokes shift of 202 nm.Moreover,its practical cell imaging capability for intracellular Ca2+ in the resting- and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.
